IN2012DN02401A - - Google Patents

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Publication number
IN2012DN02401A
IN2012DN02401A IN2401DEN2012A IN2012DN02401A IN 2012DN02401 A IN2012DN02401 A IN 2012DN02401A IN 2401DEN2012 A IN2401DEN2012 A IN 2401DEN2012A IN 2012DN02401 A IN2012DN02401 A IN 2012DN02401A
Authority
IN
India
Prior art keywords
mutated
gene
tsp
protein
ptr
Prior art date
Application number
Inventor
Mark Ellis
David Paul Humphreys
Original Assignee
Ucb Pharma Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB0916822.0A external-priority patent/GB0916822D0/en
Priority claimed from GB0916821A external-priority patent/GB0916821D0/en
Application filed by Ucb Pharma Sa filed Critical Ucb Pharma Sa
Publication of IN2012DN02401A publication Critical patent/IN2012DN02401A/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/24—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
    • C07K16/241—Tumor Necrosis Factors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/24—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
    • C07K16/244—Interleukins [IL]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/55—Fab or Fab'
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/185—Escherichia
    • C12R2001/19—Escherichia coli
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00—Technologies relating to chemical industry
    • Y02P20/50—Improvements relating to the production of bulk chemicals
    • Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

A recombinant gram-negative bacterial cell comprising one or more of the following mutated protease genes: a. a mutated Tsp gene, wherein the mutated Tsp gene encodes a Tsp protein having reduced protease activity or is a knockout mutated Tsp gene; b. a mutated ptr gene, wherein the mutated ptr gene encodes a Protease III protein having reduced protease activity or is a knockout mutated ptr gene; and c. a mutated DegP gene encoding a DegP protein having chaperone activity and reduced protease activity ; wherein the cell is isogenic to a wild-type bacterial cell except for the mutated Tsp gene and/or mutated ptr gene and/or mutated Deg P gene and optionally a polynucleotide sequence encoding a protein of interest.
IN2401DEN2012 2009-09-24 2010-09-23 IN2012DN02401A (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB0916822.0A GB0916822D0 (en) 2009-09-24 2009-09-24 Bacterial host strain
GB0916821A GB0916821D0 (en) 2009-09-24 2009-09-24 Bacterial host strain
PCT/GB2010/001790 WO2011036454A1 (en) 2009-09-24 2010-09-23 Bacterial host strain

Publications (1)

Publication Number Publication Date
IN2012DN02401A true IN2012DN02401A (en) 2015-08-21

Family

ID=43003459

Family Applications (1)

Application Number Title Priority Date Filing Date
IN2401DEN2012 IN2012DN02401A (en) 2009-09-24 2010-09-23

Country Status (21)

Country Link
US (3) US9109216B2 (en)
EP (2) EP2480661A1 (en)
JP (2) JP6132551B2 (en)
KR (1) KR101758942B1 (en)
CN (1) CN102575241B (en)
AU (2) AU2010299640B2 (en)
BR (1) BR112012006670B8 (en)
CA (1) CA2774692C (en)
CY (1) CY1120947T1 (en)
DK (1) DK2993231T3 (en)
ES (1) ES2692811T3 (en)
HR (1) HRP20181482T1 (en)
HU (1) HUE040306T2 (en)
IN (1) IN2012DN02401A (en)
LT (1) LT2993231T (en)
PL (1) PL2993231T3 (en)
PT (1) PT2993231T (en)
SG (1) SG10201606462WA (en)
SI (1) SI2993231T1 (en)
SM (1) SMT201800549T1 (en)
WO (1) WO2011036454A1 (en)

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EP2480661A1 (en) 2009-09-24 2012-08-01 UCB Pharma, S.A. Bacterial host strain
GB201000590D0 (en) 2010-01-14 2010-03-03 Ucb Pharma Sa Bacterial host strain
GB201000587D0 (en) 2010-01-14 2010-03-03 Ucb Pharma Sa Bacterial hoist strain
GB201000591D0 (en) 2010-01-14 2010-03-03 Ucb Pharma Sa Bacterial hoist strain
GB201012599D0 (en) 2010-07-27 2010-09-08 Ucb Pharma Sa Process for purifying proteins
HRP20180226T1 (en) * 2011-07-13 2018-03-09 Ucb Biopharma Sprl HOUSEHOLD BACTERIAL BATTERY EXPRESSING RECOMBINANT DSBC
GB201208367D0 (en) 2012-05-14 2012-06-27 Ucb Pharma Sa Biological product
WO2018119142A1 (en) 2016-12-21 2018-06-28 Amgen Inc. Anti-tnf alpha antibody formulations
WO2019193418A1 (en) 2018-04-05 2019-10-10 Bio-Rad Abd Serotec Gmbh Display systems for proteins of interest
US11674164B2 (en) 2019-03-18 2023-06-13 Bio-Rad Abd Serotec Gmbh Periplasmic fusion proteins
CN110616181A (en) * 2019-09-27 2019-12-27 北京理工大学 Engineering strain based on escherichia coli transformation
CN117965503B (en) * 2024-02-02 2024-08-09 南京迪诺薇华生物科技有限公司 A protease mutant and its application in skin care products and cosmetics

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Also Published As

Publication number Publication date
US9315770B2 (en) 2016-04-19
EP2993231A2 (en) 2016-03-09
US20120258492A1 (en) 2012-10-11
AU2010299640B2 (en) 2016-02-25
HK1172650A1 (en) 2013-04-26
BR112012006670B8 (en) 2022-03-03
SMT201800549T1 (en) 2018-11-09
JP2016116517A (en) 2016-06-30
JP6132551B2 (en) 2017-05-24
US9109216B2 (en) 2015-08-18
PL2993231T3 (en) 2019-01-31
EP2993231A3 (en) 2016-05-11
US20160200810A1 (en) 2016-07-14
BR112012006670B1 (en) 2021-08-03
CA2774692C (en) 2017-12-19
US9550973B2 (en) 2017-01-24
HUE040306T2 (en) 2019-03-28
WO2011036454A1 (en) 2011-03-31
AU2010299640A1 (en) 2012-04-12
CN102575241B (en) 2016-02-24
EP2993231B1 (en) 2018-08-01
HRP20181482T1 (en) 2018-11-02
CY1120947T1 (en) 2019-12-11
AU2016201536A1 (en) 2016-03-24
US20150344840A1 (en) 2015-12-03
DK2993231T3 (en) 2018-10-22
KR20120083435A (en) 2012-07-25
LT2993231T (en) 2018-11-12
CA2774692A1 (en) 2011-03-31
CN102575241A (en) 2012-07-11
BR112012006670A2 (en) 2015-09-08
ES2692811T3 (en) 2018-12-05
KR101758942B1 (en) 2017-07-17
SI2993231T1 (en) 2018-10-30
SG10201606462WA (en) 2016-09-29
EP2480661A1 (en) 2012-08-01
JP2013505714A (en) 2013-02-21
PT2993231T (en) 2018-11-12

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