IN2012DN03281A - - Google Patents

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Publication number
IN2012DN03281A
IN2012DN03281A IN3281DEN2012A IN2012DN03281A IN 2012DN03281 A IN2012DN03281 A IN 2012DN03281A IN 3281DEN2012 A IN3281DEN2012 A IN 3281DEN2012A IN 2012DN03281 A IN2012DN03281 A IN 2012DN03281A
Authority
IN
India
Prior art keywords
pyrococcus
nucleic acid
acid sequence
sequence encoding
intein segment
Prior art date
Application number
Inventor
Gerald R Carson
Wendy R Gion
Yune Z Kunes
Iii Walter F Leise
Rachel A Davis-Taber
Emma Fung
Original Assignee
Abbott Lab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Abbott Lab filed Critical Abbott Lab
Publication of IN2012DN03281A publication Critical patent/IN2012DN03281A/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/395—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A61P37/04—Immunostimulants
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/24—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
    • C07K16/241—Tumor Necrosis Factors
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/24—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
    • C07K16/244—Interleukins [IL]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/21—Immunoglobulins specific features characterized by taxonomic origin from primates, e.g. man
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/70—Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen
    • C07K2317/76—Antagonist effect on antigen, e.g. neutralization or inhibition of binding
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/90—Fusion polypeptide containing a motif for post-translational modification
    • C07K2319/92—Fusion polypeptide containing a motif for post-translational modification containing an intein ("protein splicing")domain

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Microbiology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Epidemiology (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

Embodiments of the invention relate to vector constructs and methods for expression of polypeptides including multimeric products such as therapeutic antibodies. Particular constructs allow for the generation of expression products from a single open reading frame (sORF). An embodiment provides an isolated or purified expression vector for generating one or more recombinant protein products comprising a single open reading frame insert; said insert comprising a signal peptide nucleic acid sequence encoding a signal peptide; a first nucleic acid sequence encoding a first polypeptide; a first intervening nucleic acid sequence encoding a first protein cleavage site, wherein said first protein cleavage site is provided by an intein segment of a Ion protease gene of Pyrococcus or a klbA gene of Pyrococcus or Methanococcus, or a modified intein segment derived therefrom; and a second nucleic acid sequence encoding a second polypeptide. Certain embodiments of constructs and methods employ an intein segment of a Ion protease gene of Pyrococcus abyssi, Pyrococcus furiosus, or Pyrococcus horikoshii OT3; or an intein segment of a klbA gene of Pyrococcus abyssi, Pyrococcus furiosus, or Methanococcus jannaschir, or other intein segment.
IN3281DEN2012 2009-10-30 2010-10-28 IN2012DN03281A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US25654409P 2009-10-30 2009-10-30
PCT/US2010/054475 WO2011053699A1 (en) 2009-10-30 2010-10-28 Sorf constructs and multiple gene expression

Publications (1)

Publication Number Publication Date
IN2012DN03281A true IN2012DN03281A (en) 2015-10-23

Family

ID=43431207

Family Applications (1)

Application Number Title Priority Date Filing Date
IN3281DEN2012 IN2012DN03281A (en) 2009-10-30 2010-10-28

Country Status (13)

Country Link
US (1) US20110150861A1 (en)
EP (1) EP2493923A1 (en)
JP (1) JP2013509188A (en)
KR (1) KR20120091251A (en)
CN (1) CN102812040A (en)
AU (1) AU2010313395A1 (en)
CA (1) CA2776990A1 (en)
IL (1) IL219022A0 (en)
IN (1) IN2012DN03281A (en)
MX (1) MX2012005117A (en)
RU (1) RU2012122240A (en)
TW (1) TW201124535A (en)
WO (1) WO2011053699A1 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013078433A1 (en) * 2011-11-23 2013-05-30 University Of Hawaii Auto-processing domains for polypeptide expression
EP2906599B1 (en) * 2012-10-12 2019-07-17 Agency For Science, Technology And Research Optimised heavy chain and light chain signal peptides for the production of recombinant antibody therapeutics
US11103932B2 (en) 2018-09-17 2021-08-31 Korea Institute Of Machinery & Materials Cutting head operated by centrifugal force and cutting apparatus including the same
CA3150224A1 (en) 2019-09-10 2021-03-18 Obsidian Therapeutics, Inc. Ca2 compositions and methods for tunable regulation
JP7846626B2 (en) * 2020-02-07 2026-04-15 ザ チルドレンズ メディカル センター コーポレーション Large gene vectors and methods for their delivery and use
CN114107390B (en) * 2021-11-05 2023-10-24 中国科学院精密测量科学与技术创新研究院 rAAV vector for expressing antibody IgG1 and application thereof

Family Cites Families (95)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6455275B1 (en) * 1980-02-25 2002-09-24 The Trustees Of Columbia University In The City Of New York DNA construct for producing proteinaceous materials in eucaryotic cells
US4816567A (en) 1983-04-08 1989-03-28 Genentech, Inc. Recombinant immunoglobin preparations
US4937190A (en) * 1987-10-15 1990-06-26 Wisconsin Alumni Research Foundation Translation enhancer
DE3885431D1 (en) * 1987-12-23 1993-12-09 Boehringer Ingelheim Int Expression of the virally encoded protease P2A of HRV2.
US5648254A (en) * 1988-01-15 1997-07-15 Zymogenetics, Inc. Co-expression in eukaryotic cells
US5436146A (en) * 1989-09-07 1995-07-25 The Trustees Of Princeton University Helper-free stocks of recombinant adeno-associated virus vectors
US5162601A (en) * 1989-11-22 1992-11-10 The Upjohn Company Plant potyvirus expression vector with a gene for protease
US5519164A (en) * 1990-02-01 1996-05-21 Hoechst Aktiengesellschaft Expression of a multigene RNA having self-splicing activity
US5633282A (en) * 1990-05-25 1997-05-27 British Technology Group Limited Inhibition of viral infection
US5637481A (en) * 1993-02-01 1997-06-10 Bristol-Myers Squibb Company Expression vectors encoding bispecific fusion proteins and methods of producing biologically active bispecific fusion proteins in a mammalian cell
ES2213141T3 (en) * 1992-05-22 2004-08-16 Montana State University ANTIBODIES WITH SPECIFICITY TOWARDS MULTIPLE ADHESION MOLECULES.
DE4228457A1 (en) * 1992-08-27 1994-04-28 Beiersdorf Ag Production of heterodimeric PDGF-AB using a bicistronic vector system in mammalian cells
DE4228458A1 (en) * 1992-08-27 1994-06-01 Beiersdorf Ag Multicistronic expression units and their use
US5834247A (en) * 1992-12-09 1998-11-10 New England Biolabs, Inc. Modified proteins comprising controllable intervening protein sequences or their elements methods of producing same and methods for purification of a target protein comprised by a modified protein
US5496714A (en) * 1992-12-09 1996-03-05 New England Biolabs, Inc. Modification of protein by use of a controllable interveining protein sequence
DK0678122T3 (en) 1993-01-12 2000-03-06 Biogen Inc Recombinant anti-VLA4 antibody molecules
US5532142A (en) * 1993-02-12 1996-07-02 Board Of Regents, The University Of Texas System Method of isolation and purification of fusion polypeptides
US6133028A (en) * 1993-05-28 2000-10-17 Transgene S.A. Defective adenoviruses and corresponding complementation lines
US5834256A (en) * 1993-06-11 1998-11-10 Cell Genesys, Inc. Method for production of high titer virus and high efficiency retroviral mediated transduction of mammalian cells
GB9326271D0 (en) * 1993-12-23 1994-02-23 Zeneca Ltd Expression of self-processing polyprotein in transgenic plants
US5643745A (en) * 1994-02-03 1997-07-01 University Of Hawaii Heterologous dimeric proteins produced in heterokaryons
JP3816518B2 (en) * 1994-06-10 2006-08-30 ジェンベク、インコーポレイティッド Complementary adenoviral vector systems and cell lines
FR2722208B1 (en) * 1994-07-05 1996-10-04 Inst Nat Sante Rech Med NEW INTERNAL RIBOSOME ENTRY SITE, VECTOR CONTAINING SAME AND THERAPEUTIC USE
US5955072A (en) * 1994-07-13 1999-09-21 Sankyo Company, Limited Expression systems utilizing autolyzing fusion proteins and a reducing polypeptide
JP3659261B2 (en) * 1994-10-20 2005-06-15 モルフォシス・アクチェンゲゼルシャフト Targeted heterojunction of a recombinant protein to a multifunctional complex
US20020168342A1 (en) * 1994-11-03 2002-11-14 Cell Genesys, Inc. Novel adenoviral vectors, packaging cell lines, recombinant adenoviruses and methods
US5872005A (en) 1994-11-03 1999-02-16 Cell Genesys Inc. Packaging cell lines for adeno-associated viral vectors
AT403167B (en) * 1994-11-14 1997-11-25 Immuno Ag SELECTION AND EXPRESSION OF FOREIGN PROTEINS BY MEANS OF A SELECTION-AMPLIFICATION SYSTEM
US6638762B1 (en) * 1994-11-28 2003-10-28 Genetic Therapy, Inc. Tissue-vectors specific replication and gene expression
IL116816A (en) 1995-01-20 2003-05-29 Rhone Poulenc Rorer Sa Cell for the production of a defective recombinant adenovirus or an adeno-associated virus and the various uses thereof
US5641870A (en) 1995-04-20 1997-06-24 Genentech, Inc. Low pH hydrophobic interaction chromatography for antibody purification
US5912167A (en) * 1995-06-06 1999-06-15 Wisconsin Alumni Research Foundation Autocatalytic cleavage site and use thereof in a protein expression vector
US6040183A (en) * 1995-06-07 2000-03-21 University Of North Carloina At Chapel Hill Helper virus-free AAV production
US6093570A (en) 1995-06-07 2000-07-25 The University Of North Carolina At Chapel Hill Helper virus-free AAV production
US6090382A (en) 1996-02-09 2000-07-18 Basf Aktiengesellschaft Human antibodies that bind human TNFα
DK0929578T3 (en) * 1996-02-09 2003-08-25 Abbott Lab Bermuda Ltd Human antibodies that bind human TNFalfa
DE69737597D1 (en) * 1996-07-22 2007-05-24 Us Gov Health & Human Serv VECTORS FOR INHIBITING VIRAL AND TUMOR GROWTH
US6962708B1 (en) * 1997-02-28 2005-11-08 Acambis, Inc. Chimeric flavivirus vaccines
US6849428B1 (en) * 1997-03-05 2005-02-01 New England Biolabs, Inc. Intein-mediated protein ligation of expressed proteins
US5981182A (en) 1997-03-13 1999-11-09 Albert Einstein College Of Medicine Of Yeshiva University Vector constructs for the selection and identification of open reading frames
WO1998046728A1 (en) 1997-04-14 1998-10-22 Cell Genesys, Inc. Methods for increasing the efficiency of recombinant aav product
US6933378B2 (en) * 1997-05-30 2005-08-23 Joseph Atabekov Methods for coexpression of more than one gene in eukaryotic cells
US7007589B1 (en) 1997-09-15 2006-03-07 R. Sanderson Management, Inc. Piston assembly
FR2782325B1 (en) * 1998-08-12 2002-05-24 Proteus METHOD OF IDENTIFYING POLYNUCLEOTIDE SEQUENCES AND / OR CORRESPONDING PROTEINS FROM A SAMPLE OF NUCLEIC ACIDS
US20030099932A1 (en) * 1998-05-12 2003-05-29 Lorens James B. Retroviral vectors with separation sequences
US6537806B1 (en) * 1998-06-02 2003-03-25 University Of Washington Compositions and methods for treating diabetes
US7001745B1 (en) * 1998-09-30 2006-02-21 New England Biolabs, Inc. Intein mediated peptide ligation
JP2002532066A (en) * 1998-11-16 2002-10-02 ジェンウェイ バイオテック， インコーポレイテッド Antibody production using polynucleotide vaccines in birds
CA2362737A1 (en) * 1999-03-05 2000-09-08 Maxygen, Inc. Recombination of insertion modified nucleic acids
WO2000056901A2 (en) * 1999-03-24 2000-09-28 Board Of Regents, The University Of Texas System Linear and circular expression elements
US6914128B1 (en) 1999-03-25 2005-07-05 Abbott Gmbh & Co. Kg Human antibodies that bind human IL-12 and methods for producing
US6933362B1 (en) * 1999-08-17 2005-08-23 Rensselaer Polytechnic Institute Genetic system and self-cleaving inteins derived therefrom, bioseparations and protein purification employing same, and methods for determining critical, generalizable amino acid residues for varying intein activity
CA2386180C (en) * 1999-10-13 2010-03-09 Immunex Corporation Vectors and methods for recombinant protein expression
WO2001034816A1 (en) * 1999-10-29 2001-05-17 Kosan Biosciences, Inc. Rapamycin analogs
US6665567B2 (en) * 2000-01-14 2003-12-16 Rainer R. Iraschko Optical-ring integer linear program formulation
ES2336641T3 (en) 2000-02-11 2010-04-15 Metabolix, Inc. MULTIGENIC EXPRESSION CONSTRUCTS CONTAINING MODIFIED INTEINS.
AU2001247296A1 (en) 2000-03-06 2001-09-17 Rigel Pharmaceuticals, Inc. In vivo production of cyclic peptides
US7378248B2 (en) 2000-03-06 2008-05-27 Rigel Pharmaceuticals, Inc. In vivo production of cyclic peptides for inhibiting protein-protein interaction
WO2001071004A2 (en) * 2000-03-17 2001-09-27 Incyte Genomics, Inc. Proteases
US6692736B2 (en) * 2000-03-24 2004-02-17 Cell Genesys, Inc. Cell-specific adenovirus vectors comprising an internal ribosome entry site
US6908751B2 (en) * 2000-04-26 2005-06-21 Zymogenetics, Inc. Methods for enhancing the expression of a protein of interest by recombinant host cells
US6544780B1 (en) * 2000-06-02 2003-04-08 Genphar, Inc. Adenovirus vector with multiple expression cassettes
US6852510B2 (en) * 2000-07-03 2005-02-08 Gala Design Inc Host cells containing multiple integrating vectors
US20030036643A1 (en) 2000-09-14 2003-02-20 Jin Cheng He Methods and compositions for the construction and use of fusion libraries
US7419829B2 (en) * 2000-10-06 2008-09-02 Oxford Biomedica (Uk) Limited Vector system
US20040191786A1 (en) * 2001-02-16 2004-09-30 Yue David T. Three cube fret method (3-fret) for detecting fluorescence energy transfer
MXPA03008022A (en) * 2001-03-09 2003-12-04 Genentech Inc Process for production of polypeptides.
FI116851B (en) * 2001-05-03 2006-03-15 Fit Biotech Oyj Plc Expression vector, its uses and process for its preparation and products containing it
WO2002089840A1 (en) * 2001-05-10 2002-11-14 The Regents Of The University Of California Recombinant bicistronic flaviviruses and methods of use thereof
US20040180415A1 (en) * 2001-05-15 2004-09-16 Tchaga Grigoriy S. Methods and compositions for protein purification
US7052905B1 (en) * 2001-08-13 2006-05-30 University Of Kentucky Research Foundation Methods and composition for expressing multiple genes in plants by alternate splicing of a polycistronic message
EP1288891A1 (en) * 2001-08-27 2003-03-05 Hewlett-Packard Company Process and apparatus for displaying data in a specific area of the display in a computer or in an interactive terminal under control of the LAN card and independently on the operating system
WO2003040168A2 (en) * 2001-11-06 2003-05-15 Enanta Pharmaceuticals, Inc. Methods and compositions for identifying peptide aptamers capable of altering a cell phenotype
US20030157641A1 (en) * 2001-11-16 2003-08-21 Idec Pharmaceuticals Corporation Polycistronic expression of antibodies
US20040235011A1 (en) * 2002-06-26 2004-11-25 Cooper Richard K. Production of multimeric proteins
US20050112095A1 (en) * 2002-07-09 2005-05-26 Tsu-An Hsu Internal ribosome entry sites for recombinant protein expression
CN1263860C (en) * 2002-09-30 2006-07-12 华南农业大学 Construction method of multigene carrier and its application
US20050147962A1 (en) * 2002-11-19 2005-07-07 Wagstrom Christopher R. Display of dimeric proteins on phage
EP1573058B1 (en) * 2002-12-18 2011-09-14 ChromaGenics B.V. A method for improving protein production
AU2004238843B2 (en) * 2003-05-09 2009-04-23 Research Development Foundation Insertion of furin protease cleavage sites in membrane proteins and uses thereof
US20050136035A1 (en) * 2003-06-03 2005-06-23 Derek Ko Cell specific replication-competent viral vectors comprising a self processing peptide cleavage site
WO2005017149A1 (en) * 2003-06-03 2005-02-24 Cell Genesys, Inc. Compositions and methods for enhanced expression of recombinant polypeptides from a single vector using a peptide cleavage site
US7485291B2 (en) 2003-06-03 2009-02-03 Cell Genesys, Inc. Compositions and methods for generating multiple polypeptides from a single vector using a virus derived peptide cleavage site, and uses thereof
DE602004020776D1 (en) * 2003-08-05 2009-06-04 Almac Sciences Scotland Ltd LIGATION
DE602004026343D1 (en) 2003-10-24 2010-05-12 Amgen Inc PROCESS FOR PURIFYING PROTEINS IN A FLOW FRACTION FROM CHROMATOGRAPHY WITH HYDROPHOBIC INTERACTIONS
US7968684B2 (en) 2003-11-12 2011-06-28 Abbott Laboratories IL-18 binding proteins
US20050221429A1 (en) * 2004-01-16 2005-10-06 Cardinal Health Pts, Llc Host cells containing multiple integrating vectors comprising an amplifiable marker
US20090098611A1 (en) 2004-02-27 2009-04-16 Wood David W Self-cleaving affinity tags and methods of use
US7714119B2 (en) * 2004-07-13 2010-05-11 Biosante Pharmaceuticals, Inc. AAV vector compositions and methods for enhanced expression of immunoglobulins using the same
EP2468768A3 (en) * 2005-07-21 2012-10-31 Abbott Laboratories Multiple gene expression including sorf contructs and methods with polyproteins, pro-proteins, and proteolysis
US20070041905A1 (en) * 2005-08-19 2007-02-22 Hoffman Rebecca S Method of treating depression using a TNF-alpha antibody
BRPI0617688A2 (en) * 2005-10-21 2011-06-07 Hoffmann La Roche method for recombinant expression of a polypeptide
EP1783228A1 (en) * 2005-11-08 2007-05-09 ETH Zürich Recombinant expression of multiprotein complexes using polygenes
US20130273553A9 (en) * 2007-01-10 2013-10-17 Ronald C. Geyer Stabilization of cyclic peptide structures
KR20140001229A (en) * 2007-03-30 2014-01-06 아보트 러보러터리즈 Recombinant expression vector elements(reves) for enhancing expression of recombinant proteins in host cells

Also Published As

Publication number Publication date
IL219022A0 (en) 2012-06-28
WO2011053699A1 (en) 2011-05-05
RU2012122240A (en) 2013-12-10
EP2493923A1 (en) 2012-09-05
MX2012005117A (en) 2012-06-14
US20110150861A1 (en) 2011-06-23
AU2010313395A1 (en) 2012-05-10
JP2013509188A (en) 2013-03-14
CN102812040A (en) 2012-12-05
CA2776990A1 (en) 2011-05-05
TW201124535A (en) 2011-07-16
KR20120091251A (en) 2012-08-17

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