IN2012DN03431A - - Google Patents

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Publication number
IN2012DN03431A
IN2012DN03431A IN3431DEN2012A IN2012DN03431A IN 2012DN03431 A IN2012DN03431 A IN 2012DN03431A IN 3431DEN2012 A IN3431DEN2012 A IN 3431DEN2012A IN 2012DN03431 A IN2012DN03431 A IN 2012DN03431A
Authority
IN
India
Prior art keywords
cells
container
magnetic stir
stir element
disclosed
Prior art date
Application number
Inventor
Phillip Belgrader
Benjamin Hindson
Original Assignee
Akonni Biosystems
Quantalife Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Akonni Biosystems, Quantalife Inc filed Critical Akonni Biosystems
Publication of IN2012DN03431A publication Critical patent/IN2012DN03431A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C12N1/066—Lysis of microorganisms by physical processes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Sustainable Development (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Mixers With Rotating Receptacles And Mixers With Vibration Mechanisms (AREA)

Abstract

A method for lysing cells is disclosed. The method includes stirring cells with a magnetic stir element in the presence of a plurality of cell lysis beads at a speed sufficient to lyse the cells. Also disclosed is a device for lysing cells. The device includes a container having a magnetic stir element and a plurality of cell lysis beads disposed therein. The container is dimensioned to allow rotation of the magnetic stir element inside the container.
IN3431DEN2012 2009-09-21 2010-09-20 IN2012DN03431A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US27239609P 2009-09-21 2009-09-21
PCT/US2010/002569 WO2011034621A2 (en) 2009-09-21 2010-09-20 Magnetic lysis method and device

Publications (1)

Publication Number Publication Date
IN2012DN03431A true IN2012DN03431A (en) 2015-10-23

Family

ID=43756937

Family Applications (1)

Application Number Title Priority Date Filing Date
IN3431DEN2012 IN2012DN03431A (en) 2009-09-21 2010-09-20

Country Status (8)

Country Link
US (4) US8399190B2 (en)
EP (2) EP3067112B1 (en)
JP (1) JP2013505018A (en)
CN (2) CN105969761B (en)
CA (2) CA3041540C (en)
ES (1) ES2709123T3 (en)
IN (1) IN2012DN03431A (en)
WO (1) WO2011034621A2 (en)

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Also Published As

Publication number Publication date
CN102686306B (en) 2015-11-25
CA2811872C (en) 2019-06-18
US8399190B2 (en) 2013-03-19
US20190024039A1 (en) 2019-01-24
CA3041540C (en) 2021-08-24
EP2480324A2 (en) 2012-08-01
CN102686306A (en) 2012-09-19
US20160215255A1 (en) 2016-07-28
CN105969761A (en) 2016-09-28
US10138458B2 (en) 2018-11-27
CN105969761B (en) 2020-04-07
JP2013505018A (en) 2013-02-14
US20120003654A2 (en) 2012-01-05
US20110070589A1 (en) 2011-03-24
CA2811872A1 (en) 2011-03-24
WO2011034621A3 (en) 2011-11-24
US20130157274A1 (en) 2013-06-20
WO2011034621A2 (en) 2011-03-24
US9217174B2 (en) 2015-12-22
US11118156B2 (en) 2021-09-14
ES2709123T3 (en) 2019-04-15
EP3067112A1 (en) 2016-09-14
EP2480324A4 (en) 2013-10-02
EP3067112B1 (en) 2018-12-26
CA3041540A1 (en) 2011-03-24

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