IN2012DN03823A - - Google Patents

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Publication number
IN2012DN03823A
IN2012DN03823A IN3823DEN2012A IN2012DN03823A IN 2012DN03823 A IN2012DN03823 A IN 2012DN03823A IN 3823DEN2012 A IN3823DEN2012 A IN 3823DEN2012A IN 2012DN03823 A IN2012DN03823 A IN 2012DN03823A
Authority
IN
India
Prior art keywords
modified
vacuolar sorting
vps10
sorting activity
host cell
Prior art date
Application number
Other languages
English (en)
Inventor
Michael Meehl
Heping Lin
Byung-Kwon Choi
Original Assignee
Merck Sharp & Dohme
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merck Sharp & Dohme filed Critical Merck Sharp & Dohme
Publication of IN2012DN03823A publication Critical patent/IN2012DN03823A/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Botany (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
IN3823DEN2012 2009-10-30 2010-10-25 IN2012DN03823A (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US25637909P 2009-10-30 2009-10-30
US35066810P 2010-06-02 2010-06-02
PCT/US2010/053903 WO2011053541A1 (en) 2009-10-30 2010-10-25 Methods for the production of recombinant proteins with improved secretion efficiencies

Publications (1)

Publication Number Publication Date
IN2012DN03823A true IN2012DN03823A (de) 2015-08-28

Family

ID=43922476

Family Applications (1)

Application Number Title Priority Date Filing Date
IN3823DEN2012 IN2012DN03823A (de) 2009-10-30 2010-10-25

Country Status (12)

Country Link
US (1) US20130011875A1 (de)
EP (1) EP2494053A4 (de)
JP (1) JP2013509180A (de)
KR (1) KR20140015137A (de)
CN (1) CN102686731A (de)
AU (1) AU2010313608A1 (de)
BR (1) BR112012009886A2 (de)
CA (1) CA2777487A1 (de)
IN (1) IN2012DN03823A (de)
MX (1) MX2012004993A (de)
RU (1) RU2012122166A (de)
WO (1) WO2011053541A1 (de)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2494050A4 (de) * 2009-10-30 2013-10-30 Merck Sharp & Dohme In glycoengineerter pichia pastoris hergestellter granulozytenkolonienstimulierender faktor
US9102969B2 (en) * 2011-03-11 2015-08-11 Kaneka Corporation Method for producing heterologous protein using yeast with disruption of VPS gene
DK2800809T3 (en) 2012-01-05 2018-05-22 Glykos Finland Oy PROTEASE-DEFECTED FILAMENTARY FUNGAL CELLS AND PROCEDURES FOR USE THEREOF
CN102559740B (zh) * 2012-02-25 2013-03-27 山东大学 一种提高酿酒酵母分泌表达异源蛋白的方法及专用酿酒酵母菌株
WO2013174927A1 (en) 2012-05-23 2013-11-28 Novartis International Pharmaceutical Limited Production of fucosylated glycoproteins
US20160017020A1 (en) * 2013-03-06 2016-01-21 Protalix Ltd. Chimeric polypeptides, polynucleotides encoding same, cells expressing same and methods of producing same
US10435731B2 (en) 2013-07-10 2019-10-08 Glykos Finland Oy Multiple proteases deficient filamentous fungal cells and methods of use thereof
SG11201700446XA (en) 2014-07-21 2017-02-27 Glykos Finland Oy Production of glycoproteins with mammalian-like n-glycans in filamentous fungi
WO2016079739A2 (en) * 2014-11-20 2016-05-26 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Compositions and methods for producing polypeptides with a modified glycosylation pattern in plant cells
DK3332005T3 (da) * 2015-08-05 2022-01-03 Lonza Ag Promotorvarianter
EP3568408A4 (de) * 2017-01-13 2020-12-16 Bolt Threads, Inc. Elastomere proteine
US11795487B2 (en) * 2017-07-31 2023-10-24 Melt & Marble AB Fungal cell with improved protein production capacity
SE2051317A1 (en) * 2020-11-11 2022-05-12 Nielsen Dina Petranovic A genetically modified yeast cell
CN113604373A (zh) * 2021-02-08 2021-11-05 江南大学 一种提高人源溶菌酶产量及酶活的毕赤酵母缺陷型菌株
CN113736818A (zh) * 2021-10-08 2021-12-03 江南大学 一种毕赤酵母中提高人源溶菌酶分泌效率及酶活的方法
CN113862242B (zh) * 2021-10-15 2024-06-25 江南大学 一种减弱液泡分选提高里氏木霉纤维素酶产量的方法
EP4194560A1 (de) 2021-12-08 2023-06-14 European Molecular Biology Laboratory Verbesserte produktion von sekretierten proteinen in pilzzellen
CN115851468A (zh) * 2022-07-18 2023-03-28 江南大学 一种生产人酪蛋白巨肽的重组毕赤酵母及其应用
CN116478905B (zh) * 2023-02-15 2025-07-22 北京中医药大学 一种高尔基体体外培养方法及其在构建药物毒性筛选系统中的应用
WO2025000334A1 (zh) * 2023-06-29 2025-01-02 中国科学院深圳先进技术研究院 融合蛋白及其在制备代谢产物中的应用

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4818700A (en) 1985-10-25 1989-04-04 Phillips Petroleum Company Pichia pastoris argininosuccinate lyase gene and uses thereof
DK1283265T3 (da) 2000-05-17 2009-11-09 Mitsubishi Tanabe Pharma Corp Fremgangsmåde til fremstilling af protein med reduktion af mannosephosphat i sukkerkæden og glycoprotein fremstillet derved
US8697394B2 (en) 2000-06-28 2014-04-15 Glycofi, Inc. Production of modified glycoproteins having multiple antennary structures
US7598055B2 (en) 2000-06-28 2009-10-06 Glycofi, Inc. N-acetylglucosaminyltransferase III expression in lower eukaryotes
EP2322644A1 (de) 2000-06-28 2011-05-18 GlycoFi, Inc. Verfahren für die Herstellung modifizierter Glykoproteine
US7449308B2 (en) 2000-06-28 2008-11-11 Glycofi, Inc. Combinatorial DNA library for producing modified N-glycans in lower eukaryotes
US7795002B2 (en) 2000-06-28 2010-09-14 Glycofi, Inc. Production of galactosylated glycoproteins in lower eukaryotes
US7863020B2 (en) 2000-06-28 2011-01-04 Glycofi, Inc. Production of sialylated N-glycans in lower eukaryotes
US7625756B2 (en) 2000-06-28 2009-12-01 GycoFi, Inc. Expression of class 2 mannosidase and class III mannosidase in lower eukaryotic cells
GB0121088D0 (en) * 2001-08-31 2001-10-24 Cambridge Consultants Electric kettle
JP4820055B2 (ja) 2001-12-27 2011-11-24 グライコフィ， インコーポレイテッド 哺乳動物型糖質構造を操作するための方法
IL165717A0 (en) * 2002-06-26 2006-01-15 Flanders Interuniversity Inst A strain of methylotrophic yeast for producing proteins
AU2003291448A1 (en) 2002-11-12 2004-06-03 Purdue Research Foundation Benzoate inducible promoters
EP2341128A1 (de) 2003-12-24 2011-07-06 GlycoFi, Inc. Verfahren zur Elimination der Mannosylphosphorylierung von Glykanen bei der Herstellung von Glykoproteinen
US7479389B2 (en) 2004-03-02 2009-01-20 Glycofi, Inc. ARG1, ARG2, ARG3, HIS1, HIS2, HIS5, HIS6 genes and methods for stable genetic integration
EP1730293A2 (de) 2004-03-17 2006-12-13 Glycofi, Inc. Verfahren zur konstruktion eines cytidinmonophosphat-sialinsäure-synthesewegs in pilzen und hefen
US7465577B2 (en) 2004-04-29 2008-12-16 Glycofi, Inc. Methods for reducing or eliminating α-mannosidase resistant glycans for the production of glycoproteins
CA2606253A1 (en) 2005-04-21 2006-10-26 Uti Limited Partnership Pcr for mrsa sccmec typing
CA2651456A1 (en) 2006-05-19 2007-11-29 Glycofi, Inc. Recombinant vectors
MX2008014744A (es) * 2006-05-19 2009-02-10 Glycofi Inc Composiciones de eritrocpoyetina.

Also Published As

Publication number Publication date
BR112012009886A2 (pt) 2015-09-15
US20130011875A1 (en) 2013-01-10
CA2777487A1 (en) 2011-05-05
CN102686731A (zh) 2012-09-19
EP2494053A1 (de) 2012-09-05
AU2010313608A1 (en) 2012-06-07
KR20140015137A (ko) 2014-02-06
WO2011053541A1 (en) 2011-05-05
JP2013509180A (ja) 2013-03-14
MX2012004993A (es) 2012-06-12
RU2012122166A (ru) 2013-12-10
EP2494053A4 (de) 2013-10-30

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