IN2015DN01609A - - Google Patents

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Publication number
IN2015DN01609A
IN2015DN01609A IN1609DEN2015A IN2015DN01609A IN 2015DN01609 A IN2015DN01609 A IN 2015DN01609A IN 1609DEN2015 A IN1609DEN2015 A IN 1609DEN2015A IN 2015DN01609 A IN2015DN01609 A IN 2015DN01609A
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IN
India
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presented
methods
amplicons
approaches
quantifying
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English (en)
Inventor
Imran R Malik
Xiomara L Madero
Erika F Garcia
Sheel M Shah
Axel Scherer
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California Inst Of Techn
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Publication of IN2015DN01609A publication Critical patent/IN2015DN01609A/en

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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/6486Measuring fluorescence of biological material, e.g. DNA, RNA, cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6851Quantitative amplification
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00Heating or cooling apparatus; Heat insulating devices
    • B01L7/52Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Biomedical Technology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Clinical Laboratory Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Algebra (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Mathematical Analysis (AREA)
  • Mathematical Optimization (AREA)
  • Mathematical Physics (AREA)
  • Pure & Applied Mathematics (AREA)
  • Dispersion Chemistry (AREA)
  • Time-Division Multiplex Systems (AREA)
IN1609DEN2015 2012-08-03 2013-08-02 IN2015DN01609A (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261679547P 2012-08-03 2012-08-03
PCT/US2013/053512 WO2014022827A1 (en) 2012-08-03 2013-08-02 Multiplexing and quantification in pcr with reduced hardware and requirements

Publications (1)

Publication Number Publication Date
IN2015DN01609A true IN2015DN01609A (de) 2015-07-03

Family

ID=50025861

Family Applications (1)

Application Number Title Priority Date Filing Date
IN1609DEN2015 IN2015DN01609A (de) 2012-08-03 2013-08-02

Country Status (5)

Country Link
US (3) US9791372B2 (de)
EP (2) EP3901243A1 (de)
CN (2) CN104662172B (de)
IN (1) IN2015DN01609A (de)
WO (1) WO2014022827A1 (de)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114717296B (zh) 2012-02-03 2025-04-04 加州理工学院 多路生化测定中信号的编码和解码
EP3901243A1 (de) * 2012-08-03 2021-10-27 California Institute of Technology Multiplexing und quantifizierung in pcr mit reduzierter hardware und reduzierten anforderungen
WO2015147370A1 (en) * 2014-03-28 2015-10-01 Seegene, Inc. Detection of target nucleic acid sequences using different detection temperatures
DE102014105129B3 (de) * 2014-04-10 2015-07-02 Kist Europe-Korea Institute of Science and Technologie Europe Forschungsgesellschaft mbh Verfahren und Master-Mix für die quantitative Echtzeit-PCR für Multiplex-Ziel-Nukleinsäuremoleküle
CN103884679B (zh) * 2014-04-18 2016-01-20 山西大学 结合卡尔曼滤波器的腔衰荡光谱技术气体浓度监测方法
WO2016093620A1 (en) 2014-12-09 2016-06-16 Seegene, Inc. Differentiation of signals for target nucleic acid sequences
EP3130679B1 (de) * 2015-08-13 2018-02-28 Cladiac GmbH Verfahren und testsystem zum nachweis und/oder quantifizieren einer ziel-nukleinsäure in einer probe
US10066263B2 (en) 2016-06-17 2018-09-04 California Institute Of Technology Nucleic acid reactions and related methods and compositions
US11915795B2 (en) * 2016-12-23 2024-02-27 The Regents Of The University Of California Method and device for digital high resolution melt
US20190112636A1 (en) * 2017-10-16 2019-04-18 ChromaCode, Inc. Methods and compositions for nucleic acid detection
US12454720B2 (en) 2018-04-17 2025-10-28 ChromaCode, Inc. Methods and systems for multiplex analysis
CN108841939B (zh) * 2018-06-21 2020-09-22 北京致雨生物科技有限公司 多重数字pcr浓度测量方法及微滴式数字pcr系统
US12203129B2 (en) 2018-07-03 2025-01-21 ChromaCode, Inc. Formulations and signal encoding and decoding methods for massively multiplexed biochemical assays
JP7221491B2 (ja) * 2018-09-18 2023-02-14 株式会社ミズホメディー 複数の標的核酸を検出するキットを用いる検出方法
EP3999624B1 (de) 2019-07-16 2026-03-25 Meliolabs Inc. Verfahren und vorrichtungen für einzellenbasierte digitale hochauflösende schmelzanalyse
WO2025235889A1 (en) * 2024-05-10 2025-11-13 Guardant Health, Inc. Methods involving multiplexed pooled pcr

Family Cites Families (152)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE88761T1 (de) 1986-01-10 1993-05-15 Amoco Corp Kompetitiver homogener test.
US5618711A (en) 1986-08-22 1997-04-08 Hoffmann-La Roche Inc. Recombinant expression vectors and purification methods for Thermus thermophilus DNA polymerase
US6127155A (en) 1986-08-22 2000-10-03 Roche Molecular Systems, Inc. Stabilized thermostable nucleic acid polymerase compositions containing non-ionic polymeric detergents
US5079351A (en) 1986-11-26 1992-01-07 Cetus Corporation Oligonucleotides and kits for detection of htlvi and htlvii viruses by hybridization
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
DE69213112T2 (de) 1991-06-20 1997-04-03 Hoffmann La Roche Verbesserte Methoden zur Nukleinsäure-Amplifizierung
EP0675966B1 (de) 1992-02-19 2004-10-06 The Public Health Research Institute Of The City Of New York, Inc. Neue anordnungen von oligonukleotiden und ihr nutzen zum sortieren, isolieren, sequenzierung und manipulieren von nukleinsäuren
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US5948360A (en) 1994-07-11 1999-09-07 Tekmar Company Autosampler with robot arm
WO1996024044A1 (en) 1995-02-02 1996-08-08 Chugai Seiyaku Kabushiki Kaisha Method of assaying specimen substance by controlling dose of chemiluminescence
CA2216645A1 (en) 1995-04-25 1996-11-21 Irori Remotely programmable matrices with memories and uses thereof
AU693023B2 (en) 1995-05-05 1998-06-18 Applied Biosystems, Llc Methods and reagents for combined PCR amplification and hybridization probing assay
US5882856A (en) 1995-06-07 1999-03-16 Genzyme Corporation Universal primer sequence for multiplex DNA amplification
US5773258A (en) 1995-08-25 1998-06-30 Roche Molecular Systems, Inc. Nucleic acid amplification using a reversibly inactivated thermostable enzyme
US20050112637A1 (en) 1995-09-08 2005-05-26 Chatterjee Deb K. Mutant DNA polymerases and uses thereof
US5981180A (en) 1995-10-11 1999-11-09 Luminex Corporation Multiplexed analysis of clinical specimens apparatus and methods
US6007994A (en) 1995-12-22 1999-12-28 Yale University Multiparametric fluorescence in situ hybridization
AU2324997A (en) 1996-03-15 1997-10-01 Penn State Research Foundation, The Detection of extracellular tumor-associated nucleic acid in blood plasma or ser um using nucleic acid amplification assays
DE69739909D1 (de) 1996-03-26 2010-07-29 Michael S Kopreski Methoden aus plasma oder serum extrahierte extrazelluraere rna zur diagnoseüberwachung oder evaluation von krebs verwenden
JP4540754B2 (ja) 1996-06-04 2010-09-08 ユニバーシティ オブ ユタ リサーチ ファウンデーション Pcr中のハイブリダイゼーションのモニタリング
US5834203A (en) * 1997-08-25 1998-11-10 Applied Spectral Imaging Method for classification of pixels into groups according to their spectra using a plurality of wide band filters and hardwire therefore
CA2306501C (en) 1997-10-14 2011-03-29 Luminex Corporation Precision fluorescently dyed particles and methods of making and using same
US6235480B1 (en) 1998-03-13 2001-05-22 Promega Corporation Detection of nucleic acid hybrids
WO1999052708A1 (en) 1998-04-13 1999-10-21 Luminex Corporation Liquid labeling with fluorescent microparticles
EP1090293B2 (de) 1998-06-24 2019-01-23 Illumina, Inc. Dekodierung von matrixartig-angeordneten Sensoren durch Mikropartikel
AU762728B2 (en) 1998-07-02 2003-07-03 Gen-Probe Incorporated Molecular torches
US6642062B2 (en) 1998-09-03 2003-11-04 Trellis Bioinformatics, Inc. Multihued labels
US7079241B2 (en) 2000-04-06 2006-07-18 Invitrogen Corp. Spatial positioning of spectrally labeled beads
JP2002542793A (ja) 1999-04-22 2002-12-17 ザ アルバート アインシュタイン カレッジ オブ メディシン オブ イエシバ ユニバーシティ 多蛍光fishによる遺伝子発現パターンのアッセイ法
EP1196623B8 (de) 1999-07-23 2008-05-21 Forensic Science Service Ltd Verfahren zur Erkennung von Einzel-Nukleotid-Polymorphismen
DE60030145T2 (de) 1999-10-22 2008-01-10 PHRI Properties, Inc., Newark Nachweisverfahren für kurze sequenzvarianten
US6506568B2 (en) * 2000-02-10 2003-01-14 The Penn State Research Foundation Method of analyzing single nucleotide polymorphisms using melting curve and restriction endonuclease digestion
US20040053230A1 (en) * 2001-03-07 2004-03-18 Schaffer Michael Edward Real time quantitative pcr with intercalating dye for single and multiplex target dna
EP1278889B1 (de) 2000-03-29 2006-07-26 LGC Limited Hybridisierungsprobe und methode zum schnellen nachweis und zur schnellen unterscheidung von sequenzen
CA2314398A1 (en) 2000-08-10 2002-02-10 Edward Shipwash Microarrays and microsystems for amino acid analysis and protein sequencing
US20020142345A1 (en) 2000-12-22 2002-10-03 Nelsen Anita J. Methods for encoding and decoding complex mixtures in arrayed assays
AU2002324422A1 (en) 2001-02-21 2002-12-23 Amnis Corporation Method and apparatus for labeling and analyzing cellular components
CA2439402A1 (en) 2001-03-02 2002-09-12 University Of Pittsburgh Of The Commonwealth System Of Higher Education Pcr method
WO2003003015A2 (en) 2001-06-28 2003-01-09 Advanced Research And Technology Institute, Inc. Methods of preparing multicolor quantum dot tagged beads and conjugates thereof
WO2003002979A2 (en) 2001-06-28 2003-01-09 Illumina, Inc. Multiplex decoding of array sensors with microspheres
US7473767B2 (en) 2001-07-03 2009-01-06 The Institute For Systems Biology Methods for detection and quantification of analytes in complex mixtures
EP1288308A1 (de) 2001-08-28 2003-03-05 Roche Diagnostics GmbH Verfahren zur bestimmung von mehranalyten
US6852491B2 (en) 2001-09-04 2005-02-08 Abbott Laboratories Amplification and detection reagents for HIV-1
US20030134320A1 (en) 2002-01-15 2003-07-17 Myriad Genetics, Incorporated Method system and computer program product for quality assurance in detecting biochemical markers
EP1426448A1 (de) 2002-12-03 2004-06-09 bioMérieux BV Methode zur Reduktion des Effektes von Sequenzvariationen in einem diagnostischen Hybridisierungsverfahren, Sonde zur Verwendung in einem solchen Verfahren, sowie das Verfahren selbst
CA2525482C (en) 2003-04-04 2015-02-17 F. Hoffmann-La Roche Ag Improved system for multicolor real-time pcr comprising 3 to 6 pairs of forester resonance energy transfer (fret) probes
US7385043B1 (en) 2003-04-30 2008-06-10 The Public Health Research Institute Of The City Of New York, Inc. Homogeneous multiplex screening assays and kits
US20050053950A1 (en) * 2003-09-08 2005-03-10 Enrique Zudaire Ubani Protocol and software for multiplex real-time PCR quantification based on the different melting temperatures of amplicons
US20050064435A1 (en) 2003-09-24 2005-03-24 Xing Su Programmable molecular barcodes
TWI232934B (en) 2003-11-19 2005-05-21 Ind Tech Res Inst A biochip containing splitable reaction confinement and method for producing same and application thereof
EP1689764B1 (de) 2003-11-19 2013-01-02 AlleLogic Biosciences Corporation Mit mehreren fluorophoren markierte oligonukleotide
JP2007530051A (ja) 2004-03-24 2007-11-01 アプレラ コーポレイション 標的分子を決定するためのライゲーション反応および増幅反応
US7445898B2 (en) 2004-04-01 2008-11-04 Bio-Rad Laboratories, Inc. Quantitative amplification with a labeled probe and 3′ to 5′ exonuclease activity
WO2005111243A2 (en) 2004-05-07 2005-11-24 Cepheid Multiplexed detection of biological agents
US7575864B2 (en) 2004-05-27 2009-08-18 E.I. Du Pont De Nemours And Company Method for the direct detection of diagnostic RNA
DE102004043870B4 (de) 2004-09-10 2009-11-19 Eppendorf Ag Verfahren zur Erweiterung des dynamischen Erfassungsbereich bei Mikroarrays
EP1799853A2 (de) 2004-09-24 2007-06-27 Universität Bern Molekulare beacons
RU2460804C2 (ru) * 2004-10-18 2012-09-10 Брандейс Юнивесити Способ гомогенной детекции по меньшей мере одного продукта одноцепочечной амплификации
AU2005299590B2 (en) 2004-10-27 2010-09-16 Cepheid Closed-system multi-stage nucleic acid amplification reactions
EP1838880A2 (de) 2005-01-21 2007-10-03 Third Wave Technologies, Inc. Verfahren und zusammensetzungen zur erhöhten detektion dynamischer bereiche von nukleinsäuremolekülen
US7507575B2 (en) * 2005-04-01 2009-03-24 3M Innovative Properties Company Multiplex fluorescence detection device having removable optical modules
US7709249B2 (en) * 2005-04-01 2010-05-04 3M Innovative Properties Company Multiplex fluorescence detection device having fiber bundle coupling multiple optical modules to a common detector
EP1892302A1 (de) 2005-06-16 2008-02-27 BIOTOOLS BIOTECHNOLOGICAL & MEDICAL LABORATORIES, S.A. Nukleinsäurenachweisverfahren mit direkter erzeugung eines messbaren signals
US7919242B2 (en) 2005-06-30 2011-04-05 Roche Molecular Systems, Inc. Light emission modifiers and their uses in nucleic acid detection, amplification and analysis
US7553955B2 (en) 2005-09-01 2009-06-30 The Trustees Of The University Of Pennsylvania Molecular beacons, methods and kits for detecting DNA damage response
PL1937834T3 (pl) 2005-09-01 2014-11-28 Mtpcr Pty Ltd Sposoby wzmacniania, kwantyfikacji i identyfikacji kwasów nukleinowych
CN1936019B (zh) 2005-10-18 2013-01-30 厦门大学 用于多重实时核酸扩增检测的探针编码方法
JP5700911B2 (ja) 2005-12-23 2015-04-15 ナノストリング テクノロジーズ,インコーポレーテッド 配向され、固定化された巨大分子を含む組成物とその製造法
WO2007076128A2 (en) 2005-12-23 2007-07-05 Nanostring Technologies, Inc. Nanoreporters and methods of manufacturing and use thereof
US9133506B2 (en) 2006-01-10 2015-09-15 Applied Spectral Imaging Ltd. Methods and systems for analyzing biological samples
US8455184B2 (en) 2006-04-18 2013-06-04 The United States Of America As Represented By The Secretary Of The Air Force Differential multiplexing with pattern recognition
CN101495654A (zh) 2006-04-19 2009-07-29 阿普里拉股份有限公司 无凝胶珠基测序的试剂、方法和文库
CA2653095C (en) 2006-05-22 2013-07-16 Nanostring Technologies, Inc. Systems and methods for analyzing nanoreporters
EP2210955A3 (de) 2006-05-23 2010-09-15 Molecular Detection, Inc. Bei Umgebungstemperatur beständige Kits zur Molekulardiagnostik
WO2008002882A2 (en) 2006-06-26 2008-01-03 Blood Cell Storage, Inc. Device and method for extraction and analysis of nucleic acids from biological samples
US8048626B2 (en) 2006-07-28 2011-11-01 California Institute Of Technology Multiplex Q-PCR arrays
US8187541B2 (en) 2006-09-18 2012-05-29 California Institute Of Technology Apparatus for detecting target molecules and related methods
WO2008052774A2 (en) 2006-10-31 2008-05-08 Noxxon Pharma Ag Methods for detection of a single- or double-stranded nucleic acid molecule
US9201063B2 (en) 2006-11-16 2015-12-01 General Electric Company Sequential analysis of biological samples
JP2010511390A (ja) 2006-11-30 2010-04-15 ジェン−プロウブ インコーポレイテッド 予め規定されたマスター検量線の調整を使用する定量方法
CN102851369B (zh) 2006-12-13 2015-01-21 卢米耐克斯公司 用于实时pcr多重分析的系统和方法
CA2580589C (en) 2006-12-19 2016-08-09 Fio Corporation Microfluidic detection system
WO2008109636A1 (en) 2007-03-05 2008-09-12 University Of Utah Research Foundation Methods and compositions related to nucleic acid detection
WO2008118998A2 (en) 2007-03-27 2008-10-02 Primera Biosystems Inc. Method for multiplex detection and quantitation of nucleic acids
CA2687292C (en) 2007-04-10 2017-07-04 Nanostring Technologies, Inc. Methods and computer systems for identifying target-specific sequences for use in nanoreporters
EP3620465B1 (de) 2007-07-03 2025-02-19 Dako Denmark A/S Verbesserte verfahren zur herstellung, markierung und verwendung von mhc-multimeren
EP2193356B1 (de) 2007-08-29 2014-07-23 Eppendorf Ag Vorrichtung und verfahren für die radiometrische messung mehrerer proben
WO2009036514A2 (en) 2007-09-20 2009-03-26 Molecular Plant Breeding Nominees Ltd Method of amplifying nucleic acid
EP2224017A4 (de) 2007-12-26 2011-05-04 Arkray Inc Verfahren zur erhöhung der nukleinsäure-zielsequenz und dafür verwendete sonde
EP2116614A1 (de) 2008-05-06 2009-11-11 Qiagen GmbH Gleichzeitiger Nachweis mehrerer Nukleinsäuresequenzen in einer Reaktion
GB0812862D0 (en) 2008-07-14 2008-08-20 Lumora Ltd Nucleic acid amplification
WO2010013017A1 (en) 2008-07-31 2010-02-04 Oxitec Limited Multiplex amplification and detection
CN102112632A (zh) * 2008-08-08 2011-06-29 史密斯探测公司 用于pcr分析的检测算法
US8519115B2 (en) 2008-08-14 2013-08-27 Nanostring Technologies, Inc. Stable nanoreporters
US9921154B2 (en) 2011-03-18 2018-03-20 Bio-Rad Laboratories, Inc. Multiplexed digital assays
US8951939B2 (en) 2011-07-12 2015-02-10 Bio-Rad Laboratories, Inc. Digital assays with multiplexed detection of two or more targets in the same optical channel
RU2011125332A (ru) * 2008-11-21 2012-12-27 Конинклейке Филипс Электроникс Н.В. Определение множественной пцр в реальном времени на твердых поверхностях с использованием красителей со специфичностью к двухцепочечным нуклеиновым кислотам
US8614061B2 (en) 2008-12-01 2013-12-24 Qiagen, Gmbh Combination of fluorescent dyes for the detection of nucleic acids
EP2376659B1 (de) 2008-12-17 2015-12-02 Life Technologies Corporation Verfahren, zusammensetzungen und kits für den nachweis von allelen varianten
CN102439171B (zh) * 2008-12-22 2017-07-14 犹他大学研究基金会 单色多重定量pcr
US8361738B2 (en) 2009-03-06 2013-01-29 California Institute Of Technology Methods for quantitative target detection and related devices and systems
US8039215B2 (en) 2009-03-10 2011-10-18 Roche Molecular Systems, Inc. Multiplex quantitative nucleic acid amplification and melting assay
CN101570782B (zh) 2009-03-20 2012-01-11 曹际娟 乳制品中8种致病菌检测试剂盒及其检测方法
FI20095514A0 (fi) * 2009-05-07 2009-05-07 Expression Analytics Oy Menetelmä, laitteisto ja tietokoneohjelmatuote PCR-tuotteiden kvantifioimiseksi
WO2010130877A2 (en) 2009-05-12 2010-11-18 Abacus Diagnostica Oy Method for detecting nucleic acids
CN102449166B (zh) 2009-05-26 2013-12-04 厦门大学 一种单管检测多个单核苷酸变异或单核苷酸多态性的方法
EP2436777B1 (de) 2009-05-26 2015-08-19 Xiamen University Verfahren zur erkennung von variationen in nukleinsäuresequenzen
EP2280080A1 (de) 2009-07-31 2011-02-02 Qiagen GmbH Verfahren zur standardisierten Quantifizierung von Nukleinsäuren unter Verwendung von Anker-Oligonukleotiden und Adapter-Oligonukleotiden
CN102482720B (zh) 2009-09-10 2014-10-08 3M创新有限公司 对多重核酸扩增中的光谱串扰的补偿
US9447457B2 (en) 2009-09-24 2016-09-20 Seegene, Inc. Detection of target nucleic acid sequences by cyclic exonucleolytic reactions
US8962251B2 (en) 2009-10-08 2015-02-24 Medical Service Consultation International, Llc Methods and compositions for identifying sulfur and iron modifying bacteria
WO2011047087A2 (en) 2009-10-13 2011-04-21 Nanostring Technologies, Inc. Protein detection via nanoreporters
US9752187B2 (en) 2009-12-11 2017-09-05 Nucleix Categorization of DNA samples
EP2516681B1 (de) 2010-02-11 2017-10-18 Nanostring Technologies, Inc Zusammensetzungen und verfahren zur erkennung von vorzugsweise kleiner rnas mittels brücken hybridisierung und ligation
US9366632B2 (en) 2010-02-12 2016-06-14 Raindance Technologies, Inc. Digital analyte analysis
EP4484577A3 (de) 2010-02-12 2025-03-26 Bio-Rad Laboratories, Inc. Digitale analytanalyse
US9399797B2 (en) 2010-02-12 2016-07-26 Raindance Technologies, Inc. Digital analyte analysis
US20110229888A1 (en) 2010-03-16 2011-09-22 Hengen Paul N Compositions and Methods for the Detection of Genomic Features
EP2572001A2 (de) 2010-05-19 2013-03-27 QIAGEN Gaithersburg, Inc. Verfahren und zusammensetzungen für sequenzspezifische reinigung und multiplex-analyse von nukleinsäuren
CN102269759B (zh) * 2010-06-07 2014-05-07 常州楚天生物科技有限公司 一种基于免疫pcr和dna熔解曲线分析的多重检测方法
WO2012003289A1 (en) 2010-06-30 2012-01-05 Gen-Probe Incorporated Method and apparatus for identifying analyte-containing samples using single-read determination of analyte and process control signals
US8877464B2 (en) 2010-07-29 2014-11-04 Roche Molecular Systems, Inc. Qualitative and quantitative detection of microbial nucleic acids
GB201017978D0 (en) * 2010-10-25 2010-12-08 Oxitec Ltd Multiplex amplification and detection
WO2012058638A2 (en) 2010-10-29 2012-05-03 President And Fellows Of Harvard College Nucleic acid nanostructure barcode probes
EP2665995B1 (de) 2011-01-20 2018-08-29 University of Washington through its Center for Commercialization Verfahren und systeme zur durchführung digitaler messungen
WO2012106428A2 (en) 2011-02-03 2012-08-09 University Of Florida Research Foundation, Inc. Detection methods for target dna
CN103534360A (zh) 2011-03-18 2014-01-22 伯乐生命医学产品有限公司 借助对信号的组合使用进行的多重数字分析
WO2012129115A2 (en) 2011-03-18 2012-09-27 University Of South Florida Materials and methods for profiling micrornas
US9758834B2 (en) 2011-03-28 2017-09-12 Nanostring Technologies, Inc. Compositions and methods for diagnosing cancer
CA2839705A1 (en) 2011-06-24 2012-12-27 Nanostring Technologies, Inc. Multivariate diagnostic assays and methods for using same
US10196681B2 (en) 2011-10-06 2019-02-05 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
US8858623B2 (en) 2011-11-04 2014-10-14 Valtech Cardio, Ltd. Implant having multiple rotational assemblies
CN102559868B (zh) * 2011-11-28 2014-11-05 厦门大学 一种单管定性并定量检测多个目标核酸序列的方法
EP4249605B1 (de) 2011-12-22 2024-08-28 President And Fellows Of Harvard College Verfahren zum nachweis von analyten
CN114717296B (zh) 2012-02-03 2025-04-04 加州理工学院 多路生化测定中信号的编码和解码
JP6464081B2 (ja) 2012-06-27 2019-02-06 ラトガース,ザ ステート ユニバーシティ オブ ニュー ジャージー フローサイトメトリーを用いたrna測定によるt細胞活性化のための迅速アッセイ
EP3901243A1 (de) * 2012-08-03 2021-10-27 California Institute of Technology Multiplexing und quantifizierung in pcr mit reduzierter hardware und reduzierten anforderungen
GB201218909D0 (en) 2012-10-22 2012-12-05 Univ Singapore Assay for the parallel detection of biological material based on PCR
EP4570924A3 (de) 2013-01-24 2025-09-03 California Institute of Technology Chromophorbasierte charakterisierungs- und nachweisverfahren
EP2971095B1 (de) 2013-03-12 2019-11-20 Life Technologies Corporation Verfahren für universelle reporterbasierte genotypisierung, und reaktionsmischungen und kits zur deren durchführung
EP2873740A1 (de) 2013-11-19 2015-05-20 Oncodiag Verfahren zur Überwachung, Diagnose und Untersuchung von Blasenkrebs
US9670530B2 (en) 2014-01-30 2017-06-06 Illumina, Inc. Haplotype resolved genome sequencing
WO2015147370A1 (en) 2014-03-28 2015-10-01 Seegene, Inc. Detection of target nucleic acid sequences using different detection temperatures
JP6681841B2 (ja) 2014-05-09 2020-04-15 ライフコーデックス アーゲー 特別な細胞タイプに由来するdnaの検出とそれに関連する方法
EP4155397B1 (de) 2016-02-10 2024-06-26 The Regents of The University of Michigan Nachweis von nukleinsäuren
WO2018081178A1 (en) 2016-10-24 2018-05-03 Two Pore Guys, Inc. Fractional abundance of polynucleotide sequences in a sample
WO2017173035A1 (en) 2016-04-01 2017-10-05 Chromacode Inc. Competitive probes for engineering signal generation
US10066263B2 (en) 2016-06-17 2018-09-04 California Institute Of Technology Nucleic acid reactions and related methods and compositions
CN111406119A (zh) 2017-06-28 2020-07-10 克罗玛科德公司 使用微滴式pcr系统的多重荧光测量
US20190112636A1 (en) 2017-10-16 2019-04-18 ChromaCode, Inc. Methods and compositions for nucleic acid detection
US20200407794A1 (en) 2018-02-28 2020-12-31 ChromaCode, Inc. Molecular targets for fetal nucleic acid analysis

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CN108753932A (zh) 2018-11-06
EP2880184B1 (de) 2021-03-31
EP3901243A1 (de) 2021-10-27
US11959856B2 (en) 2024-04-16
CN104662172B (zh) 2018-07-06
US20240201090A1 (en) 2024-06-20
CN104662172A (zh) 2015-05-27
US9791372B2 (en) 2017-10-17
EP2880184A4 (de) 2016-03-30
EP2880184A1 (de) 2015-06-10
US20140038195A1 (en) 2014-02-06
CN108753932B (zh) 2022-12-02

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