IN2015DN03341A - - Google Patents
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- Publication number
- IN2015DN03341A IN2015DN03341A IN3341DEN2015A IN2015DN03341A IN 2015DN03341 A IN2015DN03341 A IN 2015DN03341A IN 3341DEN2015 A IN3341DEN2015 A IN 3341DEN2015A IN 2015DN03341 A IN2015DN03341 A IN 2015DN03341A
- Authority
- IN
- India
- Prior art keywords
- monosaccharide
- microorganism
- acceptor substrate
- reaction
- free
- Prior art date
Links
- 150000002772 monosaccharides Chemical class 0.000 abstract 5
- 244000005700 microbiome Species 0.000 abstract 3
- 239000000758 substrate Substances 0.000 abstract 2
- 102000005744 Glycoside Hydrolases Human genes 0.000 abstract 1
- 108010031186 Glycoside Hydrolases Proteins 0.000 abstract 1
- 102000051366 Glycosyltransferases Human genes 0.000 abstract 1
- 108700023372 Glycosyltransferases Proteins 0.000 abstract 1
- 230000003197 catalytic effect Effects 0.000 abstract 1
- 238000006243 chemical reaction Methods 0.000 abstract 1
- 238000006460 hydrolysis reaction Methods 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 239000002773 nucleotide Substances 0.000 abstract 1
- 125000003729 nucleotide group Chemical group 0.000 abstract 1
- 239000006228 supernatant Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/02—Monosaccharides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
- C12N9/1051—Hexosyltransferases (2.4.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
The present invention relates to a process for producing a monosaccharide using a microorganism. The microorganism possesses a glycosyltransferase and a glycosidase which work together to synthesise a desired monosaccharide in free form by using an endogenous provided nucleotide activated monosaccharide , glycosylate a suitable acceptor substrate and release the desired monosaccharide by a hydrolysis reaction. The required acceptor substrate for the reaction is recycled and only needed in catalytic amounts. The monosaccharide is produced in free from and is retrieved from the supernatant of the cultivated microorganism.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP12190801.6A EP2728009B1 (en) | 2012-10-31 | 2012-10-31 | Process for producing monosaccharides |
| PCT/EP2013/068579 WO2014067696A1 (en) | 2012-10-31 | 2013-09-09 | Process for producing monosacchcarides |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| IN2015DN03341A true IN2015DN03341A (en) | 2015-10-23 |
Family
ID=47148622
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| IN3341DEN2015 IN2015DN03341A (en) | 2012-10-31 | 2013-09-09 |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US9938549B2 (en) |
| EP (1) | EP2728009B1 (en) |
| JP (1) | JP6165872B2 (en) |
| KR (1) | KR101812018B1 (en) |
| CN (1) | CN104822839B (en) |
| DK (1) | DK2728009T3 (en) |
| ES (1) | ES2640978T3 (en) |
| IN (1) | IN2015DN03341A (en) |
| WO (1) | WO2014067696A1 (en) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK3041946T4 (en) * | 2013-09-06 | 2025-11-03 | Glycom As | Fermentative production of oligosaccharides |
| PL2845905T3 (en) | 2013-09-10 | 2021-09-27 | Chr. Hansen HMO GmbH | Production of oligosaccharides |
| ES3002778T3 (en) | 2014-06-27 | 2025-03-07 | Glycom As | Oligosaccharide production |
| US11926858B2 (en) | 2014-06-27 | 2024-03-12 | Glycom A/S | Oligosaccharide production |
| EP3050973A1 (en) | 2015-01-30 | 2016-08-03 | Jennewein Biotechnologie GmbH | Fermentation process for producing monosaccharides in free form from nucleotide-activated sugars |
| ES2856749T3 (en) * | 2016-10-29 | 2021-09-28 | Chr Hansen Hmo Gmbh | Process for the production of fucosylated oligosaccharides |
| EP3486326A1 (en) * | 2017-11-21 | 2019-05-22 | Jennewein Biotechnologie GmbH | Method for the purification of n-acetylneuraminic acid from a fermentation broth |
| US12077792B2 (en) * | 2018-05-15 | 2024-09-03 | The Board Of Trustees Of The University Of Illinois | Engineered microorganisms for production of 2′fucosyllactose and l-fucose |
| EP3569713A1 (en) * | 2018-05-16 | 2019-11-20 | Jennewein Biotechnologie GmbH | Use of glycosidases in the production of oligosaccharides |
| WO2022013143A1 (en) | 2020-07-13 | 2022-01-20 | Glycom A/S | Oligosaccharide production |
| US20250320535A1 (en) | 2021-05-17 | 2025-10-16 | Dsm Ip Assets B.V. | Novel technology to enable sucrose utilization in strains for biosynthetic production |
| CN113699087B (en) * | 2021-08-23 | 2023-08-15 | 齐鲁工业大学 | Lactobacillus plantarum engineering strain for converting lactose to generate lactulose, construction method and application thereof |
| WO2026022036A1 (en) | 2024-07-22 | 2026-01-29 | Chr. Hansen A/S | Method for manufacturing l-fucose powder by spray-drying and spray-dried l-fucose powder |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2000351790A (en) | 1999-06-07 | 2000-12-19 | Yaizu Suisankagaku Industry Co Ltd | Method for producing fucose-containing oligosaccharide or composition thereof and fucose-containing oligosaccharide or composition thereof |
| FR2796082B1 (en) * | 1999-07-07 | 2003-06-27 | Centre Nat Rech Scient | PROCESS FOR PRODUCING OLIGOSACCHARIDES |
| WO2005055944A2 (en) * | 2003-12-05 | 2005-06-23 | Cincinnati Children's Hospital Medical Center | Oligosaccharide compositions and use thereof in the treatment of infection |
| KR101054362B1 (en) * | 2008-07-03 | 2011-08-05 | 재단법인 목암생명공학연구소 | How to reduce the fucose content of recombinant protein |
| RU2584599C2 (en) * | 2008-12-19 | 2016-05-20 | Дженневейн Биотехнологие Гмбх | Synthesis of fucosylated compounds |
| WO2010101158A1 (en) * | 2009-03-02 | 2010-09-10 | 住友商事株式会社 | Novel gene derived from clostridium cellulovorans and use thereof |
| IT1405680B1 (en) | 2010-09-13 | 2014-01-24 | Inalco Spa | PROCESS FOR THE PRODUCTION OF L-FUCOSIUM. |
| EP2479263B1 (en) * | 2011-01-20 | 2013-11-27 | Jennewein Biotechnologie GmbH | Novel Fucosyltransferases and their applications |
| DE12746649T1 (en) * | 2011-02-16 | 2016-09-29 | Glycosyn LLC | Biosynthesis of human milcholigosaccarides in engineered bacteria |
-
2012
- 2012-10-31 EP EP12190801.6A patent/EP2728009B1/en active Active
- 2012-10-31 ES ES12190801.6T patent/ES2640978T3/en active Active
- 2012-10-31 DK DK12190801.6T patent/DK2728009T3/en active
-
2013
- 2013-09-09 CN CN201380057191.9A patent/CN104822839B/en active Active
- 2013-09-09 IN IN3341DEN2015 patent/IN2015DN03341A/en unknown
- 2013-09-09 KR KR1020157011590A patent/KR101812018B1/en active Active
- 2013-09-09 WO PCT/EP2013/068579 patent/WO2014067696A1/en not_active Ceased
- 2013-09-09 JP JP2015538345A patent/JP6165872B2/en active Active
-
2015
- 2015-04-29 US US14/700,038 patent/US9938549B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| US9938549B2 (en) | 2018-04-10 |
| JP2015532832A (en) | 2015-11-16 |
| ES2640978T3 (en) | 2017-11-07 |
| CN104822839B (en) | 2019-04-12 |
| EP2728009B1 (en) | 2017-07-26 |
| CN104822839A (en) | 2015-08-05 |
| WO2014067696A1 (en) | 2014-05-08 |
| JP6165872B2 (en) | 2017-07-19 |
| EP2728009A1 (en) | 2014-05-07 |
| KR20150097466A (en) | 2015-08-26 |
| DK2728009T3 (en) | 2017-11-06 |
| US20150240277A1 (en) | 2015-08-27 |
| KR101812018B1 (en) | 2018-01-25 |
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