IT1255697B - Metodo per l'espressione nel lievito schizosaccharomyces pombe di recettori del sistema nervoso umano - Google Patents

Metodo per l'espressione nel lievito schizosaccharomyces pombe di recettori del sistema nervoso umano

Info

Publication number
IT1255697B
IT1255697B ITMI922243A ITMI922243A IT1255697B IT 1255697 B IT1255697 B IT 1255697B IT MI922243 A ITMI922243 A IT MI922243A IT MI922243 A ITMI922243 A IT MI922243A IT 1255697 B IT1255697 B IT 1255697B
Authority
IT
Italy
Prior art keywords
expression
receptors
nervous system
pumps
human nervous
Prior art date
Application number
ITMI922243A
Other languages
English (en)
Original Assignee
Enichem Spa
Donegani Guido Ist
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Enichem Spa, Donegani Guido Ist filed Critical Enichem Spa
Priority to ITMI922243A priority Critical patent/IT1255697B/it
Publication of ITMI922243A0 publication Critical patent/ITMI922243A0/it
Priority to DK93202756.8T priority patent/DK0590721T3/da
Priority to EP93202756A priority patent/EP0590721B1/en
Priority to DE69303685T priority patent/DE69303685T2/de
Priority to AT93202756T priority patent/ATE140484T1/de
Publication of ITMI922243A1 publication Critical patent/ITMI922243A1/it
Application granted granted Critical
Publication of IT1255697B publication Critical patent/IT1255697B/it

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70571—Receptors; Cell surface antigens; Cell surface determinants for neuromediators, e.g. serotonin receptor, dopamine receptor

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Mycology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Toxicology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Neurology (AREA)
  • Cell Biology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

Metodo per l'espressione di recettori del sistema nervoso umano nelle cellule del microorganismo eucariota Schizosaccharomyces pombe tramite l'introduzione di un vettore di clonaggio modificato nel microorganismo stesso comprendente:a) costruzione di vettori per il clonaggio e l'espressione dei geni umani per i recettori del sistema nervoso;b) inserimento di tali vettori in un ceppo di lievito appropriato.
ITMI922243A 1992-09-29 1992-09-29 Metodo per l'espressione nel lievito schizosaccharomyces pombe di recettori del sistema nervoso umano IT1255697B (it)

Priority Applications (5)

Application Number Priority Date Filing Date Title
ITMI922243A IT1255697B (it) 1992-09-29 1992-09-29 Metodo per l'espressione nel lievito schizosaccharomyces pombe di recettori del sistema nervoso umano
DK93202756.8T DK0590721T3 (da) 1992-09-29 1993-09-24 Fremgangsmåde til ekspression af receptorer fra det humane nervesystem i gæren Schirosaccharomyces pombe
EP93202756A EP0590721B1 (en) 1992-09-29 1993-09-24 Method for expressing receptors of the human nervous system in the yeast Schizosaccharomyces pombe
DE69303685T DE69303685T2 (de) 1992-09-29 1993-09-24 Verfahren zur Expression von Rezeptoren des menschlichen Nervensystems in der Hefe Schizosaccharomyces Pombe
AT93202756T ATE140484T1 (de) 1992-09-29 1993-09-24 Verfahren zur expression von rezeptoren des menschlichen nervensystems in der hefe schizosaccharomyces pombe

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ITMI922243A IT1255697B (it) 1992-09-29 1992-09-29 Metodo per l'espressione nel lievito schizosaccharomyces pombe di recettori del sistema nervoso umano

Publications (3)

Publication Number Publication Date
ITMI922243A0 ITMI922243A0 (it) 1992-09-29
ITMI922243A1 ITMI922243A1 (it) 1994-03-29
IT1255697B true IT1255697B (it) 1995-11-10

Family

ID=11364027

Family Applications (1)

Application Number Title Priority Date Filing Date
ITMI922243A IT1255697B (it) 1992-09-29 1992-09-29 Metodo per l'espressione nel lievito schizosaccharomyces pombe di recettori del sistema nervoso umano

Country Status (5)

Country Link
EP (1) EP0590721B1 (it)
AT (1) ATE140484T1 (it)
DE (1) DE69303685T2 (it)
DK (1) DK0590721T3 (it)
IT (1) IT1255697B (it)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AUPM516994A0 (en) * 1994-04-20 1994-05-12 Gene Shears Pty. Limited An in vivo gene expression system
WO1996039441A1 (en) * 1995-06-06 1996-12-12 Human Genome Sciences, Inc. Human g-protein receptor hibef51
FR2746814B1 (fr) * 1996-03-26 1998-06-12 Commissariat Energie Atomique Sequences nucleiques recombinees codant pour des proteines comprenant au moins un domaine hydrophobe et leurs applications
AU1009499A (en) * 1999-01-08 2000-11-16 Johnson & Johnson Research Pty. Limited Eukaryotic cell-based system for identifying gene modulators

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2091490C1 (ru) * 1985-10-25 1997-09-27 Зимодженетикс, Инк. Способ получения гетерологичного полипептида в эукариотических микроорганизмов
IT1252444B (it) * 1990-09-07 1995-06-16 Enichem Spa Metodo per l'identificazione e/o l'isolamento di geni che codificano per recettori di membrana.

Also Published As

Publication number Publication date
DE69303685T2 (de) 1997-01-02
EP0590721B1 (en) 1996-07-17
DE69303685D1 (de) 1996-08-22
DK0590721T3 (da) 1996-10-14
EP0590721A1 (en) 1994-04-06
ITMI922243A1 (it) 1994-03-29
ITMI922243A0 (it) 1992-09-29
ATE140484T1 (de) 1996-08-15

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Legal Events

Date Code Title Description
0001 Granted
TA Fee payment date (situation as of event date), data collected since 19931001

Effective date: 19970917