IT1262899B - Processo per l'isolamento di fattore ix, fattore x e fattore ii altamente purificati dal complesso protrombinico o dal plasma umano - Google Patents

Processo per l'isolamento di fattore ix, fattore x e fattore ii altamente purificati dal complesso protrombinico o dal plasma umano

Info

Publication number
IT1262899B
IT1262899B ITFI920078A ITFI920078A IT1262899B IT 1262899 B IT1262899 B IT 1262899B IT FI920078 A ITFI920078 A IT FI920078A IT FI920078 A ITFI920078 A IT FI920078A IT 1262899 B IT1262899 B IT 1262899B
Authority
IT
Italy
Prior art keywords
factor
human plasma
complex
protrombinic
insulation
Prior art date
Application number
ITFI920078A
Other languages
English (en)
Inventor
Silvana Arrighi
Enzo Bucci
Original Assignee
Sclavo Spa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sclavo Spa filed Critical Sclavo Spa
Priority to ITFI920078A priority Critical patent/IT1262899B/it
Publication of ITFI920078A0 publication Critical patent/ITFI920078A0/it
Priority to AT93104633T priority patent/ATE184018T1/de
Priority to DE69326227T priority patent/DE69326227T2/de
Priority to EP93104633A priority patent/EP0617049B1/en
Priority to ES93104633T priority patent/ES2138603T3/es
Priority to US08/038,942 priority patent/US5378365A/en
Publication of ITFI920078A1 publication Critical patent/ITFI920078A1/it
Application granted granted Critical
Publication of IT1262899B publication Critical patent/IT1262899B/it

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/647—Blood coagulation factors not provided for in a preceding group or according to more than one of the proceeding groups
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/745—Blood coagulation or fibrinolysis factors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6429—Thrombin (3.4.21.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21005—Thrombin (3.4.21.5)

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Hematology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Compounds Of Unknown Constitution (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

E' descritto un processo per la purificazione di Fattore IX, Fattore X e Fattore II a partire da plasma umano, o sue frazioni, in cui una soluzione contenente fattori costituenti il complesso protrombinico è purificata utilizzando cromatografie a scambio ionico ed una cromatografia ad assorbimento su ioni metallici.
ITFI920078A 1992-03-22 1992-03-27 Processo per l'isolamento di fattore ix, fattore x e fattore ii altamente purificati dal complesso protrombinico o dal plasma umano IT1262899B (it)

Priority Applications (6)

Application Number Priority Date Filing Date Title
ITFI920078A IT1262899B (it) 1992-03-27 1992-03-27 Processo per l'isolamento di fattore ix, fattore x e fattore ii altamente purificati dal complesso protrombinico o dal plasma umano
AT93104633T ATE184018T1 (de) 1992-03-27 1993-03-22 Verfahren zur isolation von hoch angereicherten faktoren ix, x und ii vom prothrombin komplex oder menschlichem plasma
DE69326227T DE69326227T2 (de) 1992-03-27 1993-03-22 Verfahren zur Isolation von hoch angereicherten Faktoren IX, X und II vom Prothrombin komplex oder menschlichem Plasma
EP93104633A EP0617049B1 (en) 1992-03-22 1993-03-22 Process for the isolation of highly purified factors IX, X and II from prothrombin complex or human plasma
ES93104633T ES2138603T3 (es) 1992-03-27 1993-03-22 Procedimiento para el aislamiento de los factores ix, x y ii altamente purificados a partir del complejo protrombinico o del plasma humano.
US08/038,942 US5378365A (en) 1992-03-27 1993-03-26 Process for the isolation of highly purified factors IX, X and II from prothrombin complex or human plasma

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ITFI920078A IT1262899B (it) 1992-03-27 1992-03-27 Processo per l'isolamento di fattore ix, fattore x e fattore ii altamente purificati dal complesso protrombinico o dal plasma umano

Publications (3)

Publication Number Publication Date
ITFI920078A0 ITFI920078A0 (it) 1992-03-27
ITFI920078A1 ITFI920078A1 (it) 1993-09-27
IT1262899B true IT1262899B (it) 1996-07-22

Family

ID=11350034

Family Applications (1)

Application Number Title Priority Date Filing Date
ITFI920078A IT1262899B (it) 1992-03-22 1992-03-27 Processo per l'isolamento di fattore ix, fattore x e fattore ii altamente purificati dal complesso protrombinico o dal plasma umano

Country Status (6)

Country Link
US (1) US5378365A (it)
EP (1) EP0617049B1 (it)
AT (1) ATE184018T1 (it)
DE (1) DE69326227T2 (it)
ES (1) ES2138603T3 (it)
IT (1) IT1262899B (it)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3336631A1 (de) * 1983-10-08 1985-04-18 Behringwerke Ag, 3550 Marburg Verfahren zur pasteurisierung von plasma oder von konzentraten der blutgerinnungsfaktoren ii, vii, ix und x
DE19504852C2 (de) * 1995-02-15 2003-01-23 Octapharma Ag Lachen Verfahren zur Reinigung Vitamin K-abhängiger Gerinnungsfaktoren durch Affinitäts-Chromatographie
US5714583A (en) * 1995-06-07 1998-02-03 Genetics Institute, Inc. Factor IX purification methods
SI0796623T1 (en) * 1996-03-20 2005-10-31 Baxter Aktiengesellschaft Pharmaceutical preparation for the treatment of blood coagulation disorders
DE19625642A1 (de) * 1996-06-26 1998-01-08 Max Planck Gesellschaft Verfahren zur Herstellung einer Antidotausgangssubstanz für natürliche und synthetische Thrombininhibitoren
AT409334B (de) * 1997-09-19 2002-07-25 Immuno Ag Pharmazeutisches präparat enthaltend vitamin k-abhängige einzelfaktoren
ITBO20010426A1 (it) * 2001-07-06 2003-01-06 Alfa Wassermann Spa Processo per la purificazione di proteine farmacologicamente attive mediante cromatografia in scambio cationico
SE0203551D0 (sv) * 2002-12-02 2002-12-02 Biovitrum Ab Prothrombin purification
GB0614315D0 (en) 2006-07-19 2006-08-30 Ge Healthcare Bio Sciences Ab Chromatography columns, systems and methods
US9597610B2 (en) * 2007-03-09 2017-03-21 Ge Healthcare Bioprocess R&D Ab Packing system and method for chromatography columns
GB0704603D0 (en) * 2007-03-09 2007-04-18 Ge Healthcare Bio Sciences Ab Packing system and method for chromatography columns
US9597611B2 (en) 2007-03-09 2017-03-21 Ge Healthcare Bioprocess R&D Ab Packing system and method for chromatography columns
GB0710321D0 (en) * 2007-05-30 2007-07-11 Nhs Blood & Transplant Method
US9956272B2 (en) 2007-05-30 2018-05-01 Bio Products Laboratory Limited Methods for preparing factor X, activated factor X, inactivated factor X and inactivated factor Xa, and pharmaceutical compositions comprising same
US7934704B2 (en) * 2007-10-16 2011-05-03 Environmental Dynamics, Inc. Retrievable diffuser module with internal ballast/buoyancy chamber
JP5788803B2 (ja) * 2008-12-02 2015-10-07 ノヴォ・ノルディスク・ヘルス・ケア・アーゲー ポリペプチド精製
US9896677B2 (en) 2010-01-18 2018-02-20 Novo Nordisk Health Care Ag Purification of blood coagulation factors
EP2652491B1 (en) 2010-12-15 2017-11-29 Baxalta GmbH Eluate collection using conductivity gradient
EP2691411B1 (en) 2011-03-29 2020-02-26 GlaxoSmithKline LLC Buffer system for protein purification
US9663553B2 (en) 2014-01-29 2017-05-30 Hemarus Therapeutics Limited Integrated process for the production of therapeutics (human albumin, immunoglobulins, clotting factor VIII and clotting factor IX) from human plasma

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AT368883B (de) * 1980-07-22 1982-11-25 Immuno Ag Verfahren zur herstellung einer neuen blutgerinnungsfoerdernden praeparation auf basis von humanproteinen
DE3043857A1 (de) * 1980-11-21 1982-07-08 Behringwerke Ag, 3550 Marburg Verfahren zur herstellung von blutgerinnungsfaktoren und danach hergestellte praeparation der faktoren ii und vii
US4470968A (en) * 1983-01-13 1984-09-11 Miles Laboratories, Inc. Pasteurized therapeutically active blood coagulation factor concentrates
US4720385A (en) * 1983-03-29 1988-01-19 Miles Laboratories, Inc. Protein compositions substantially free from infectious agents
AT399095B (de) * 1986-03-27 1995-03-27 Vukovich Thomas Dr Verfahren zur auftrennung von proteinen mittels gradientenelution und vorrichtung zur durchführung des verfahrens
GB8729822D0 (en) * 1987-12-22 1988-02-03 Central Blood Lab Authority Chemical process
US4981952A (en) * 1988-10-04 1991-01-01 Eli Lilly And Company Method for the purification of vitamin K-dependent proteins
DE3914869C1 (it) * 1989-05-05 1990-08-09 Biotest Pharma Gmbh, 6072 Dreieich, De
US5138034A (en) * 1989-07-12 1992-08-11 The Green Cross Corporation Method of fractionating plasma proteins
US5286849A (en) * 1992-07-14 1994-02-15 Alpha Therapeutic Corporation Purification of factor IX

Also Published As

Publication number Publication date
ITFI920078A0 (it) 1992-03-27
ITFI920078A1 (it) 1993-09-27
EP0617049B1 (en) 1999-09-01
DE69326227D1 (de) 1999-10-07
ATE184018T1 (de) 1999-09-15
EP0617049A1 (en) 1994-09-28
ES2138603T3 (es) 2000-01-16
US5378365A (en) 1995-01-03
DE69326227T2 (de) 2000-02-17

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Effective date: 19970427