IT1271437B - Saggio per la determinazione di un antigene/anticorpo in un campione di prova e corredo per l'attuazione del saggio - Google Patents

Saggio per la determinazione di un antigene/anticorpo in un campione di prova e corredo per l'attuazione del saggio

Info

Publication number
IT1271437B
IT1271437B ITMI930477A ITMI930477A IT1271437B IT 1271437 B IT1271437 B IT 1271437B IT MI930477 A ITMI930477 A IT MI930477A IT MI930477 A ITMI930477 A IT MI930477A IT 1271437 B IT1271437 B IT 1271437B
Authority
IT
Italy
Prior art keywords
antigen
antibody
test
determination
test sample
Prior art date
Application number
ITMI930477A
Other languages
English (en)
Inventor
Paolo Falagiani
Giovanni Mistrello
Original Assignee
Lofarma Farma Lab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lofarma Farma Lab filed Critical Lofarma Farma Lab
Publication of ITMI930477A0 publication Critical patent/ITMI930477A0/it
Priority to ITMI930477A priority Critical patent/IT1271437B/it
Priority to ES93203717T priority patent/ES2063732T3/es
Priority to AT93203717T priority patent/ATE249627T1/de
Priority to EP93203717A priority patent/EP0615128B1/en
Priority to DE69333201T priority patent/DE69333201T2/de
Priority to DE0615128T priority patent/DE615128T1/de
Priority to JP6005696A priority patent/JPH06273420A/ja
Priority to CN94101155.0A priority patent/CN1125985C/zh
Priority to BR9400447A priority patent/BR9400447A/pt
Publication of ITMI930477A1 publication Critical patent/ITMI930477A1/it
Priority to GR940300091T priority patent/GR940300091T1/el
Application granted granted Critical
Publication of IT1271437B publication Critical patent/IT1271437B/it
Priority to HK98114067.2A priority patent/HK1012710B/en

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56905—Protozoa
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/551—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being inorganic
    • G01N33/553—Metal or metal coated
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56961—Plant cells or fungi

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Inorganic Chemistry (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Il metodo di saggio di tipo sandwich, per la determinazione di un antigene o anticorpo in un campione di prova, in cui il campione di prova, sospettato di contenere detto antigene o anticorpo, è posto a contatto con un supporto solido avente legato alla sua superficie un anticorpo, rispettivamente antigene, suscettibile di formare un immunocomplesso con detto antigene o rispettivamente anticorpo e in cui l'avvenuta formazione di detto complesso è rivelata con addizione di un antigene o rispettivamente anticorpo marcato, si caratterizza per il fatto che detto supporto solido è posto a contatto simultaneamente con detto campione di prova e con detto anticorpo o rispettivamente antigene marcato.Il metodo è particolarmente vantaggioso per la determinazione di allergeni ambientali e anticorpi generati da malattie infettive e parassitarie.
ITMI930477A 1993-03-12 1993-03-12 Saggio per la determinazione di un antigene/anticorpo in un campione di prova e corredo per l'attuazione del saggio IT1271437B (it)

Priority Applications (11)

Application Number Priority Date Filing Date Title
ITMI930477A IT1271437B (it) 1993-03-12 1993-03-12 Saggio per la determinazione di un antigene/anticorpo in un campione di prova e corredo per l'attuazione del saggio
ES93203717T ES2063732T3 (es) 1993-03-12 1993-12-29 Prueba para la determinacion de un antigeno/anticuerpo en una muestra de prueba y equipo para realizar la prueba.
AT93203717T ATE249627T1 (de) 1993-03-12 1993-12-29 Vorrichtung und test zur bestimmung eines antigens oder eines antikörpers in einer testprobe
EP93203717A EP0615128B1 (en) 1993-03-12 1993-12-29 Equipment and test for determining an antigen/antibody in a test sample
DE69333201T DE69333201T2 (de) 1993-03-12 1993-12-29 Vorrichtung und Test zur Bestimmung eines Antigens oder eines Antikörpers in einer Testprobe
DE0615128T DE615128T1 (de) 1993-03-12 1993-12-29 Vorrichtung und Test zur Bestimmung eines Antigens oder eines Antikörpers in einer Testprobe.
JP6005696A JPH06273420A (ja) 1993-03-12 1994-01-24 試験サンプルにおける抗原／抗体の測定のための試験及び該試験を実施するための装置
CN94101155.0A CN1125985C (zh) 1993-03-12 1994-01-29 一种用于测定试验样品中的螨虫抗原的夹层式检测方法
BR9400447A BR9400447A (pt) 1993-03-12 1994-02-07 Processo de teste tipo sanfuíche para determinar um antígeno ou anticorpo em uma amostra de teste e equipamento diagnóstico para realizar o teste
GR940300091T GR940300091T1 (en) 1993-03-12 1995-01-31 Equipment and test for determining an antigen/antibody in a test sample.
HK98114067.2A HK1012710B (en) 1993-03-12 1998-12-18 Equipment and test for determining an antigen/antibody in a test sample

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ITMI930477A IT1271437B (it) 1993-03-12 1993-03-12 Saggio per la determinazione di un antigene/anticorpo in un campione di prova e corredo per l'attuazione del saggio

Publications (3)

Publication Number Publication Date
ITMI930477A0 ITMI930477A0 (it) 1993-03-12
ITMI930477A1 ITMI930477A1 (it) 1994-09-12
IT1271437B true IT1271437B (it) 1997-05-28

Family

ID=11365339

Family Applications (1)

Application Number Title Priority Date Filing Date
ITMI930477A IT1271437B (it) 1993-03-12 1993-03-12 Saggio per la determinazione di un antigene/anticorpo in un campione di prova e corredo per l'attuazione del saggio

Country Status (9)

Country Link
EP (1) EP0615128B1 (it)
JP (1) JPH06273420A (it)
CN (1) CN1125985C (it)
AT (1) ATE249627T1 (it)
BR (1) BR9400447A (it)
DE (2) DE69333201T2 (it)
ES (1) ES2063732T3 (it)
GR (1) GR940300091T1 (it)
IT (1) IT1271437B (it)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9601404D0 (en) * 1996-01-24 1996-03-27 Indoor Biotechnologies Ltd Allergen assay
EP1920239B1 (en) * 2005-07-27 2010-07-07 Cytosignet, Inc. Detection and measurement of blood-feeding activity
CN101833000A (zh) * 2010-05-13 2010-09-15 北京海瑞祥天生物科技有限公司 高效级联放大的抗体检测法
CN103217524B (zh) * 2012-12-28 2015-07-01 新疆维吾尔自治区实验动物研究中心 适用于检测泡型包虫病抗体的囊型包虫病囊液脱酯抗原及其制备方法

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU602694B2 (en) * 1986-06-09 1990-10-25 Ortho Diagnostic Systems Inc. Improved colloidal gold membrane assay
US4962023A (en) * 1987-06-22 1990-10-09 Louisiana State University, Agricultural And Mechanical College Single incubation immuno sorbent assay method using particle labels to detect test antigen specific antibodies in presence of other antibodies
US4853335A (en) * 1987-09-28 1989-08-01 Olsen Duane A Colloidal gold particle concentration immunoassay
FR2664613B1 (fr) * 1990-07-12 1994-08-26 Pasteur Institut Sequence peptidique immunogene d'echinococcus granulosus, sequence d'adn codant pour cette sequence peptidique et applications diagnostiques et therapeutiques.
JP2516842B2 (ja) * 1991-02-06 1996-07-24 アサヒビール株式会社 ダニ抗原に対するモノクロ―ナル抗体とその利用
CA2125769C (en) * 1991-12-13 1999-01-26 Morgan Van Aken Agglutination assays and kits employing colloidal dyes

Also Published As

Publication number Publication date
EP0615128A3 (en) 1995-06-28
BR9400447A (pt) 1994-11-01
CN1098784A (zh) 1995-02-15
ES2063732T3 (es) 2004-05-16
GR940300091T1 (en) 1995-01-31
EP0615128A2 (en) 1994-09-14
DE69333201D1 (de) 2003-10-16
ES2063732T1 (es) 1995-01-16
HK1012710A1 (en) 1999-08-06
DE69333201T2 (de) 2004-04-08
ATE249627T1 (de) 2003-09-15
CN1125985C (zh) 2003-10-29
EP0615128B1 (en) 2003-09-10
ITMI930477A1 (it) 1994-09-12
ITMI930477A0 (it) 1993-03-12
JPH06273420A (ja) 1994-09-30
DE615128T1 (de) 1995-06-29

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