ITRM20050252A1 - Proteine chimeriche per la misurazione delle concentrazioni di atp nello spazio pericellulare e metodo di screening relativo. - Google Patents

Proteine chimeriche per la misurazione delle concentrazioni di atp nello spazio pericellulare e metodo di screening relativo.

Info

Publication number
ITRM20050252A1
ITRM20050252A1 IT000252A ITRM20050252A ITRM20050252A1 IT RM20050252 A1 ITRM20050252 A1 IT RM20050252A1 IT 000252 A IT000252 A IT 000252A IT RM20050252 A ITRM20050252 A IT RM20050252A IT RM20050252 A1 ITRM20050252 A1 IT RM20050252A1
Authority
IT
Italy
Prior art keywords
measurement
screening method
atp concentrations
pericellular space
chemical proteins
Prior art date
Application number
IT000252A
Other languages
English (en)
Inventor
Virgilio Francesco Di
Simonetta Falzoni
Patrizia Pellegatti
Paolo Pinton
Rosario Rizzuto
Original Assignee
Aequotech S R L
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Aequotech S R L filed Critical Aequotech S R L
Priority to IT000252A priority Critical patent/ITRM20050252A1/it
Priority to EP06745361A priority patent/EP1888623A1/en
Priority to US11/920,913 priority patent/US20100015603A1/en
Priority to CA002608852A priority patent/CA2608852A1/en
Priority to PCT/IT2006/000383 priority patent/WO2006126231A1/en
Publication of ITRM20050252A1 publication Critical patent/ITRM20050252A1/it

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/008Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions for determining co-enzymes or co-factors, e.g. NAD, ATP
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0069Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/01Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/035Fusion polypeptide containing a localisation/targetting motif containing a signal for targeting to the external surface of a cell, e.g. to the outer membrane of Gram negative bacteria, GPI- anchored eukaryote proteins
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/61Fusion polypeptide containing an enzyme fusion for detection (lacZ, luciferase)
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00Screening for compounds of potential therapeutic value

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
IT000252A 2005-05-23 2005-05-23 Proteine chimeriche per la misurazione delle concentrazioni di atp nello spazio pericellulare e metodo di screening relativo. ITRM20050252A1 (it)

Priority Applications (5)

Application Number Priority Date Filing Date Title
IT000252A ITRM20050252A1 (it) 2005-05-23 2005-05-23 Proteine chimeriche per la misurazione delle concentrazioni di atp nello spazio pericellulare e metodo di screening relativo.
EP06745361A EP1888623A1 (en) 2005-05-23 2006-05-22 Chimeric proteins for measuring atp concentrations in pericellular space and related screening method
US11/920,913 US20100015603A1 (en) 2005-05-23 2006-05-22 Chimeric proteins for measuring atp concentrations in pericellular space and related screening method
CA002608852A CA2608852A1 (en) 2005-05-23 2006-05-22 Chimeric proteins for measuring atp concentrations in pericellular space and related screening method
PCT/IT2006/000383 WO2006126231A1 (en) 2005-05-23 2006-05-22 Chimeric proteins for measuring atp concentrations in pericellular space and related screening method

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
IT000252A ITRM20050252A1 (it) 2005-05-23 2005-05-23 Proteine chimeriche per la misurazione delle concentrazioni di atp nello spazio pericellulare e metodo di screening relativo.

Publications (1)

Publication Number Publication Date
ITRM20050252A1 true ITRM20050252A1 (it) 2006-11-24

Family

ID=36954875

Family Applications (1)

Application Number Title Priority Date Filing Date
IT000252A ITRM20050252A1 (it) 2005-05-23 2005-05-23 Proteine chimeriche per la misurazione delle concentrazioni di atp nello spazio pericellulare e metodo di screening relativo.

Country Status (5)

Country Link
US (1) US20100015603A1 (it)
EP (1) EP1888623A1 (it)
CA (1) CA2608852A1 (it)
IT (1) ITRM20050252A1 (it)
WO (1) WO2006126231A1 (it)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN120683228A (zh) 2019-12-13 2025-09-23 中外制药株式会社 检测细胞外嘌呤受体配体的系统和导入该系统的非人动物
WO2025088503A1 (en) * 2023-10-26 2025-05-01 Università degli Studi di Ferrara Ratiometric probe for the measurement of extracellular atp

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SE428379B (sv) * 1978-05-31 1983-06-27 Lkb Produkter Ab Bioluminiscens bestemning av atp och reagens herfor
GB9903767D0 (en) * 1999-02-18 1999-04-14 Univ Glasgow Receptor assay

Also Published As

Publication number Publication date
US20100015603A1 (en) 2010-01-21
WO2006126231A1 (en) 2006-11-30
CA2608852A1 (en) 2006-11-30
EP1888623A1 (en) 2008-02-20

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