JO2429B1 - Methods for The Recombinant Production of Antifusogenic Peptides - Google Patents
Methods for The Recombinant Production of Antifusogenic PeptidesInfo
- Publication number
- JO2429B1 JO2429B1 JO2003151A JOP20030151A JO2429B1 JO 2429 B1 JO2429 B1 JO 2429B1 JO 2003151 A JO2003151 A JO 2003151A JO P20030151 A JOP20030151 A JO P20030151A JO 2429 B1 JO2429 B1 JO 2429B1
- Authority
- JO
- Jordan
- Prior art keywords
- peptide
- recombinant production
- methods
- antifusogenic
- inclusion bodies
- Prior art date
Links
- 108090000765 processed proteins & peptides Proteins 0.000 title abstract 8
- 238000004519 manufacturing process Methods 0.000 title abstract 2
- 238000000034 method Methods 0.000 title abstract 2
- 102000004196 processed proteins & peptides Human genes 0.000 title 1
- 210000003000 inclusion body Anatomy 0.000 abstract 3
- 210000004027 cell Anatomy 0.000 abstract 1
- 239000003795 chemical substances by application Substances 0.000 abstract 1
- 238000003776 cleavage reaction Methods 0.000 abstract 1
- 238000002955 isolation Methods 0.000 abstract 1
- 230000000813 microbial effect Effects 0.000 abstract 1
- 108020004707 nucleic acids Proteins 0.000 abstract 1
- 150000007523 nucleic acids Chemical class 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
- 230000007017 scission Effects 0.000 abstract 1
- 230000003381 solubilizing effect Effects 0.000 abstract 1
- 238000005406 washing Methods 0.000 abstract 1
Landscapes
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
A process for the recombinant production of an antifusogenic peptide by expression of a nucleic acid enconding said antifusogenic peptide as repeat peptide in a microbial host cell , isolation of inclusion bodies containing said repeat peptide , after cleavage , characterized by washing the inclusion bodies with a denaturing agent at a PH value of or below PH 6.5 solubilizing said inclusion bodies containing the repeat peptide at a PH value of at least PH 9 and cleaving said repeat peptide to obtain said antifusogenic peptide.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP02025618 | 2002-11-19 | ||
| EP30009880 | 2003-01-17 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JO2429B1 true JO2429B1 (en) | 2008-04-17 |
Family
ID=48040982
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JO2003151A JO2429B1 (en) | 2002-11-19 | 2003-11-19 | Methods for The Recombinant Production of Antifusogenic Peptides |
Country Status (1)
| Country | Link |
|---|---|
| JO (1) | JO2429B1 (en) |
-
2003
- 2003-11-19 JO JO2003151A patent/JO2429B1/en active
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