JOP20220134A1 - طريقة لتحديد محتوى هيالورونات الصوديوم في هيدروجيل - Google Patents

طريقة لتحديد محتوى هيالورونات الصوديوم في هيدروجيل

Info

Publication number
JOP20220134A1
JOP20220134A1 JOP/2022/0134A JOP20220134A JOP20220134A1 JO P20220134 A1 JOP20220134 A1 JO P20220134A1 JO P20220134 A JOP20220134 A JO P20220134A JO P20220134 A1 JOP20220134 A1 JO P20220134A1
Authority
JO
Jordan
Prior art keywords
solution
preparing
reagent
test
hydrogel
Prior art date
Application number
JOP/2022/0134A
Other languages
English (en)
Inventor
Roberto Mocchi
Original Assignee
Ub Care S R L
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ub Care S R L filed Critical Ub Care S R L
Publication of JOP20220134A1 publication Critical patent/JOP20220134A1/ar

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N31/00—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods
    • G01N31/22—Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods using chemical indicators
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/38—Diluting, dispersing or mixing samples
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4022—Concentrating samples by thermal techniques; Phase changes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4055—Concentrating samples by solubility techniques
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
    • G01N2001/4094—Concentrating samples by other techniques involving separation of suspended solids using ultrasound

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)

Abstract

أ) تحضير محلول رباعي بورات الصوديوم في حمض الكبريتيك، ككاشف أ؛ ب) تحضير الكاشف ب بإذابة الكاربازول في الإيثانول؛ ج) تحضير محاليل الاختبار بإذابة الهيدروجيل في محلول مائي؛ د) معالجة محلول الاختبار بالموجات فوق الصوتية لفترة زمنية كافية للحصول على محلول متجانس جهرياً؛ ه) تحضير محلول مخزون مرجعي بإذابة حمض الجلوكورونيك أو مادة تحتوي على حمض الجلوكورونيك في محلول مائي؛ و) تحضير 3 محاليل مرجعية على الأقل بتخفيف محلول المخزون المرجعي في محلول مائي، ويفضل عند تركيز يتراوح ما بين 0.0005? وزن/حجم و 0.0100? وزن / حجم ، ويفضل بين 0.0010? وزن / حجم و 0.0050? وزن / حجم؛ ز) تحضير أنابيب الاختبار بواسطة خلط الكاشف أ، والكاشف ب، وواحد مما يلي: محلول مرجعي، محلول اختبار، محلول مائي (خام)، واختياريا محلول للتداخل (عينة ربط تشابكي أو عينة مضافة)؛ وضع كل أنبوب اختبار في حمام مائي لمدة 5 دقائق على الأقل، ثم تبريده إلى درجة حرارة الغرفة؛ ح) قراءة الامتصاصية عند طول موجي يتراوح ما بين 500 و580 نانومتر، ويفضل عند حوالي 530 نانومتر، مقابل الخام واختيارياً عينة للتداخل.
JOP/2022/0134A 2019-12-02 2022-06-01 طريقة لتحديد محتوى هيالورونات الصوديوم في هيدروجيل JOP20220134A1 (ar)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IT102019000022626A IT201900022626A1 (it) 2019-12-02 2019-12-02 Metodo per la determinazione del contenuto di sodio ialuronato in un idrogel
PCT/EP2020/083926 WO2021110593A1 (en) 2019-12-02 2020-11-30 Method for the determination of sodium hyaluronate content in a hydrogel

Publications (1)

Publication Number Publication Date
JOP20220134A1 true JOP20220134A1 (ar) 2023-01-30

Family

ID=70154884

Family Applications (1)

Application Number Title Priority Date Filing Date
JOP/2022/0134A JOP20220134A1 (ar) 2019-12-02 2022-06-01 طريقة لتحديد محتوى هيالورونات الصوديوم في هيدروجيل

Country Status (14)

Country Link
US (1) US20230003701A1 (ar)
EP (1) EP4070090B1 (ar)
JP (1) JP7565356B2 (ar)
KR (1) KR20220108131A (ar)
CN (1) CN114829929B (ar)
AU (1) AU2020395484A1 (ar)
BR (1) BR112022010515A2 (ar)
CA (1) CA3160197A1 (ar)
ES (1) ES2956066T3 (ar)
HU (1) HUE063034T2 (ar)
IT (1) IT201900022626A1 (ar)
JO (1) JOP20220134A1 (ar)
PL (1) PL4070090T3 (ar)
WO (1) WO2021110593A1 (ar)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113917011B (zh) * 2021-09-29 2024-07-02 泉州海关综合技术服务中心 一种透明质酸钠含量的检测方法及其用途
CN115372295B (zh) * 2022-09-23 2023-03-14 北京渼颜空间生物医药有限公司 一种高效检测透明质酸组合物含量的方法
CN115932083B (zh) * 2022-11-24 2024-11-15 华熙生物科技股份有限公司 交联玻璃酸钠中甘油和1,4-丁二醇的检测方法

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2005793A1 (en) * 1988-12-19 1990-06-19 Prachya Kongtawelert Method for the detection and quantification of hyaluronan
US20020192205A1 (en) * 2001-05-11 2002-12-19 Francis Michon Immunogenic compositions of low molecular weight hyaluronic acid and methods to prevent, treat and diagnose infections and diseases caused by group A and group C streptococci
CN102648214B (zh) * 2009-12-08 2015-02-18 丘比株式会社 纯化透明质酸类的制造方法
CN105254779B (zh) * 2015-10-13 2017-11-21 青海大学 一种大分子量透明质酸的提取纯化方法
CN106053369A (zh) * 2016-07-13 2016-10-26 浙江景嘉医疗科技有限公司 一种医用交联透明质酸钠凝胶中游离透明质酸钠含量的检测方法
CN107653280B (zh) * 2016-07-25 2021-05-04 安琪酵母股份有限公司 透明质酸发酵用的组合物、培养基和方法及其应用
CN108801952A (zh) * 2018-06-13 2018-11-13 浙江景嘉医疗科技有限公司 一种医用交联透明质酸钠凝胶游离透明质酸钠含量的检测方法
CN109628363B (zh) * 2019-01-03 2022-12-02 南开大学 一株生产高分子量透明质酸的工程菌及其构建方法和应用
CN110327366A (zh) * 2019-08-13 2019-10-15 福建拓烯新材料科技有限公司 一种含有玻尿酸的美容用药物组合物

Also Published As

Publication number Publication date
PL4070090T3 (pl) 2023-11-27
CN114829929A (zh) 2022-07-29
CA3160197A1 (en) 2021-06-10
KR20220108131A (ko) 2022-08-02
EP4070090C0 (en) 2023-06-07
ES2956066T3 (es) 2023-12-12
EP4070090B1 (en) 2023-06-07
WO2021110593A1 (en) 2021-06-10
US20230003701A1 (en) 2023-01-05
JP7565356B2 (ja) 2024-10-10
JP2023504488A (ja) 2023-02-03
AU2020395484A1 (en) 2022-06-16
BR112022010515A2 (pt) 2022-08-16
CN114829929B (zh) 2025-03-07
EP4070090A1 (en) 2022-10-12
HUE063034T2 (hu) 2023-12-28
IT201900022626A1 (it) 2021-06-02

Similar Documents

Publication Publication Date Title
Hullin et al. The determination of lactic acid in microgram quantities
BR112022010515A2 (pt) Método para a determinação do teor de ácido hialurônico de um hidrogel
CN104535712A (zh) 一种快速检测亚硝酸盐的试纸及其标准比色卡与应用
CN105238848B (zh) 一种α-L-岩藻糖苷酶检测试剂盒及制备方法
Chamkouri et al. Development of a novel pH sensor based upon Janus Green B immobilized on triacetyl cellulose membrane: Experimental design and optimization
CN105463059A (zh) 一种猪肉中过氧化物酶的快速定量检测方法
KR101204542B1 (ko) 아질산성 질소 농도 검출 방법
KR102189037B1 (ko) 망간 농도 검출 방법
KR101346664B1 (ko) 질산성 질소 농도 검출방법
CN105044057B (zh) 一种利用石墨烯量子点与纳米金检测l‑半胱氨酸浓度的方法
CN110736655A (zh) 一种细胞核dna染色方法
JP2012088203A (ja) 次亜塩素酸塩および次亜臭素酸塩の個別定量法
KR20200068183A (ko) 망간 농도 검출 시약 및 검출 키트
FI20165270A7 (fi) Menetelmä ja järjestelmä tärkin määrittämiseksi näytteestä
Lhotka et al. Differential staining of tissue in the block with picric acid and the Feulgen reaction
CN101545896A (zh) pH试纸用于检测含氯消毒剂有效氯含量的用途
US3490874A (en) Colorimetric method for the quantitative determination of oxalacetic acid
BR112023026262A2 (pt) Processo para produzir cobre metálico granular
RU2014144009A (ru) Способ количественного определения произвольных имидазола (группы имидазолина)
Malins et al. Possible nitrosation of amines in smoked chub
CN101906463A (zh) 一种检测生物膜纤维素酶活性的分析方法
KR900016504A (ko) 한쪽면의 전기 도금 강판의 제조 방법
GEP20257758B (en) Process for direct sulfation of polysaccharides in an ecologically acceptable solvent
PL426928A1 (pl) Sposób adsorpcji barwnika anionowego Orange II z roztworu wodnego z użyciem modyfikowanego ditlenku tytanu
CN120064232A (zh) 一种水质游离氯浓度测定方法