JP2000500874A - 時間分解蛍光測定を使用する蛍光分子群識別のためのシステム - Google Patents

時間分解蛍光測定を使用する蛍光分子群識別のためのシステム

Info

Publication number
JP2000500874A
JP2000500874A JP10511284A JP51128498A JP2000500874A JP 2000500874 A JP2000500874 A JP 2000500874A JP 10511284 A JP10511284 A JP 10511284A JP 51128498 A JP51128498 A JP 51128498A JP 2000500874 A JP2000500874 A JP 2000500874A
Authority
JP
Japan
Prior art keywords
molecules
sample
time interval
fluorescence
time
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP10511284A
Other languages
English (en)
Japanese (ja)
Other versions
JP2000500874A5 (2
Inventor
ミュラー、ラルフ
ザウアー、マルクス
ツァンダー、クリストフ
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Boehringer Mannheim GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Mannheim GmbH filed Critical Boehringer Mannheim GmbH
Publication of JP2000500874A publication Critical patent/JP2000500874A/ja
Publication of JP2000500874A5 publication Critical patent/JP2000500874A5/ja
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6408—Fluorescence; Phosphorescence with measurement of decay time, time resolved fluorescence

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
JP10511284A 1996-08-29 1997-08-27 時間分解蛍光測定を使用する蛍光分子群識別のためのシステム Pending JP2000500874A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE19634873A DE19634873A1 (de) 1996-08-29 1996-08-29 System zur Unterscheidung fluoreszierender Molekülgruppen durch zeitaufgelöste Fluoreszenzmessung
DE19634873.0 1996-08-29
PCT/EP1997/004665 WO1998009154A1 (de) 1996-08-29 1997-08-27 System zur unterscheidung fluoreszierender molekülgruppen durch zeitaufgelöste fluoreszenzmessung

Publications (2)

Publication Number Publication Date
JP2000500874A true JP2000500874A (ja) 2000-01-25
JP2000500874A5 JP2000500874A5 (2) 2005-04-07

Family

ID=7803983

Family Applications (1)

Application Number Title Priority Date Filing Date
JP10511284A Pending JP2000500874A (ja) 1996-08-29 1997-08-27 時間分解蛍光測定を使用する蛍光分子群識別のためのシステム

Country Status (5)

Country Link
US (1) US6140048A (2)
EP (1) EP0857300A1 (2)
JP (1) JP2000500874A (2)
DE (1) DE19634873A1 (2)
WO (1) WO1998009154A1 (2)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2012042467A (ja) * 2010-08-13 2012-03-01 Leica Microsystems Cms Gmbh 光学装置の光路において検知信号を分離する方法

Families Citing this family (60)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7498164B2 (en) 1998-05-16 2009-03-03 Applied Biosystems, Llc Instrument for monitoring nucleic acid sequence amplification reaction
ATE425448T1 (de) 1998-05-16 2009-03-15 Applera Corp Optisches gerät insbesondere zur überwachung von dns-polymerasekettenreaktionen
US6818437B1 (en) 1998-05-16 2004-11-16 Applera Corporation Instrument for monitoring polymerase chain reaction of DNA
US6608671B2 (en) 1998-07-17 2003-08-19 Vertex Pharmaceuticals (San Diego) Llc Detector and screening device for ion channels
US6349160B2 (en) 1998-07-24 2002-02-19 Aurora Biosciences Corporation Detector and screening device for ion channels
WO2000009753A1 (en) * 1998-08-11 2000-02-24 Caliper Technologies Corp. Methods and systems for sequencing dna by distinguishing the decay times of fluorescent probes
US6716394B2 (en) 1998-08-11 2004-04-06 Caliper Technologies Corp. DNA sequencing using multiple fluorescent labels being distinguishable by their decay times
GB9827912D0 (en) * 1998-12-19 1999-02-10 Univ Manchester Detecting nucleic acids
DE19915137C2 (de) * 1999-03-26 2001-10-18 Michael Schaefer Verfahren zur Quantifizierung mehrerer Fluorochrome in einer mehrfach gefärbten Probe bei der Fluoreszenzmikroskopie und Verwendungen des Verfahrens
US6342701B1 (en) * 1999-07-08 2002-01-29 International Business Machines Corporation Time correlated photon counting
DE69940524D1 (de) * 1999-11-09 2009-04-16 Raytheon Co Abbildungssystem zum nachweis von fluoreszierenden organischen substanzen in einer probe
DE19956620A1 (de) 1999-11-25 2001-05-31 Zeiss Carl Jena Gmbh Verfahren zur Erfassung von Fluoreszenzerscheinungen in einem Mikroskop
DE10005844A1 (de) * 2000-02-10 2004-07-22 Andresen, Karin Anordnung zur Charakterisierung biochemischer Reaktionen und zur Analyse biologischer Proben
JP4459390B2 (ja) 2000-06-08 2010-04-28 浜松ホトニクス株式会社 蛍光測定方法、蛍光測定装置及びそれを用いた試料評価装置
DE10040988A1 (de) 2000-08-22 2002-03-21 Evotec Biosystems Ag Verfahren und Vorrichtung zum Messen chemischer und/oder biologischer Proben
DK1311829T3 (da) * 2000-08-22 2008-10-27 Evotec Ag Fremgangsmåde og anordning til måling af kemiske og/eller biologiske pröver
US20090241217A9 (en) * 2001-05-22 2009-09-24 Jingrui Wu Yield-improved transgenic plants
US7280207B2 (en) 2001-07-25 2007-10-09 Applera Corporation Time-delay integration in a flow cytometry system
US7265833B2 (en) * 2001-07-25 2007-09-04 Applera Corporation Electrophoretic system with multi-notch filter and laser excitation source
CA2453390A1 (en) 2001-07-25 2003-02-06 Applera Corporation Time-delay integration in electrophoretic detection systems
GB0118995D0 (en) * 2001-08-03 2001-09-26 Univ Wales Medicine Detection of mutations in nucleic acids
CA2463420A1 (en) * 2001-10-24 2003-05-01 Singulex, Inc. Methods for detecting genetic haplotypes by interaction with probes
US6633043B2 (en) 2002-01-30 2003-10-14 Ezzat M. Hegazi Method for characterization of petroleum oils using normalized time-resolved fluorescence spectra
US7456954B2 (en) * 2003-06-20 2008-11-25 The Regents Of The University Of California Modulated excitation fluorescence analysis
WO2005019419A2 (en) * 2003-07-31 2005-03-03 Singulex, Inc. Co-detection of single polypeptide and polynucleotide molecules
US20080021674A1 (en) * 2003-09-30 2008-01-24 Robert Puskas Methods for Enhancing the Analysis of Particle Detection
US20050136448A1 (en) * 2003-10-02 2005-06-23 Dakota Technologies, Inc. Apparatus and methods for fluorescent detection of nucleic acids
AU2005290314A1 (en) * 2004-09-28 2006-04-06 Singulex, Inc. System and method for spectroscopic analysis of single particles
US8685711B2 (en) 2004-09-28 2014-04-01 Singulex, Inc. Methods and compositions for highly sensitive detection of molecules
US7572640B2 (en) * 2004-09-28 2009-08-11 Singulex, Inc. Method for highly sensitive detection of single protein molecules labeled with fluorescent moieties
US9040305B2 (en) * 2004-09-28 2015-05-26 Singulex, Inc. Method of analysis for determining a specific protein in blood samples using fluorescence spectrometry
US20100003715A1 (en) * 2004-12-21 2010-01-07 Francesco Pellegrino Biological agent detection and identification system
JP2008541139A (ja) * 2005-05-04 2008-11-20 スリーエム イノベイティブ プロパティズ カンパニー 蛍光検出で使用されるパルス光源用のシステムおよび方法
DE102005022880B4 (de) * 2005-05-18 2010-12-30 Olympus Soft Imaging Solutions Gmbh Trennung spektral oder farblich überlagerter Bildbeiträge in einem Mehrfarbbild, insbesondere in transmissionsmikroskopischen Mehrfarbbildern
WO2008018905A2 (en) * 2006-01-17 2008-02-14 Cellumen, Inc. Method for predicting biological systems responses
CA2648385C (en) 2006-04-04 2020-09-01 Singulex, Inc. Highly sensitive system and methods for analysis of troponin
US7838250B1 (en) 2006-04-04 2010-11-23 Singulex, Inc. Highly sensitive system and methods for analysis of troponin
CN101484806A (zh) 2006-05-17 2009-07-15 协乐民公司 一种对组织进行自动分析的方法
WO2008060483A2 (en) * 2006-11-10 2008-05-22 Cellumen, Inc. Protein-protein interaction biosensors and methods of use thereof
GB0720202D0 (en) 2007-10-16 2007-11-28 Cambridge Entpr Ltd Microfluidic systems
US9267918B2 (en) 2007-10-16 2016-02-23 Cambridge Enterprise Limited Microfluidic systems
CA2709217C (en) 2007-12-19 2021-01-05 Singulex, Inc. Scanning analyzer for single molecule detection and methods of use
WO2009126380A2 (en) * 2008-03-05 2009-10-15 Singulex, Inc. Methods and compositions for highly sensitive detection of molecules
GB2464183A (en) * 2008-09-19 2010-04-14 Singulex Inc Sandwich assay
DE102009010563A1 (de) 2009-02-16 2010-08-26 Matthias W. Engel Vorrichtung zum Nachweis von Analyten in Körperflüssigkeiten
JP5678045B2 (ja) * 2009-06-08 2015-02-25 シンギュレックス・インコーポレイテッド 高感度バイオマーカーパネル
CN102985803A (zh) 2010-02-19 2013-03-20 加利福尼亚太平洋生物科学股份有限公司 集成的分析系统和方法
EP2566971B1 (en) 2010-05-06 2019-03-27 Singulex, Inc. Methods for diagnosing, staging, predicting risk for developing and identifying treatment responders for rheumatoid arthritis
US8486717B2 (en) 2011-01-18 2013-07-16 Symbolics, Llc Lateral flow assays using two dimensional features
ES2739623T3 (es) 2011-03-17 2020-02-03 Cernostics Inc Sistemas y composiciones para diagnosticar el esófago de Barrett y métodos para usarlos
DE102011055272B4 (de) 2011-11-11 2021-08-12 Presens Precision Sensing Gmbh Verfahren zur Bestimmung eines relaxationszeitabhängigen Parameters zu einem System
US9874556B2 (en) 2012-07-18 2018-01-23 Symbolics, Llc Lateral flow assays using two dimensional features
US20140072959A1 (en) 2012-09-12 2014-03-13 Force Diagnostics, Inc. Rapid tests for insurance underwriting
EP2962104B1 (en) 2013-02-26 2018-04-04 Astute Medical, Inc. Lateral flow assay device with test strip retainer
DE102013109010B4 (de) 2013-08-21 2019-03-14 Presens - Precision Sensing Gmbh Verfahren zur Bestimmung einer Veränderlichen einer Probe
EP3044592B1 (en) 2013-09-13 2019-07-17 Symbolics, LLC Lateral flow assays using two dimensional test and control signal readout patterns
WO2016164365A1 (en) 2015-04-06 2016-10-13 Bludiagnostics, Inc. A test device for detecting an analyte in a saliva sample and method of use
US20170016051A1 (en) * 2015-07-15 2017-01-19 Orizhan Bioscience Limited DNA Methylation Detection
US20210278403A1 (en) 2016-08-23 2021-09-09 Qoolabs, Inc. Lateral flow assay for assessing recombinant protein expression or reporter gene expression
CN117015700A (zh) * 2023-05-24 2023-11-07 深圳湾实验室 一种同时检测多种时间分辨荧光物质的方法和系统

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6435268A (en) * 1987-06-16 1989-02-06 Wallac Oy Assay of biologically specific multiple object to be analyzed
JPH01227948A (ja) * 1988-03-08 1989-09-12 Japan Spectroscopic Co マルチチャンネル蛍光減衰波形測定装置
JPH02234050A (ja) * 1989-03-08 1990-09-17 Hamamatsu Photonics Kk 光波形測定装置
JPH05126739A (ja) * 1991-10-31 1993-05-21 Hamamatsu Photonics Kk 核酸塩基識別法
JPH06148076A (ja) * 1992-11-10 1994-05-27 Hamamatsu Photonics Kk 核酸の塩基配列決定方法、単一分子検出方法、その装置及び試料の作成方法
JPH07505467A (ja) * 1992-03-23 1995-06-15 ダイアトロン・コーポレイション フルオロメーター検出システム

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4877965A (en) * 1985-07-01 1989-10-31 Diatron Corporation Fluorometer
US4745285A (en) * 1986-08-21 1988-05-17 Becton Dickinson And Company Multi-color fluorescence analysis with single wavelength excitation
US4923819A (en) * 1987-03-27 1990-05-08 Chimerix Corporation Time-resolved fluorescence immunoassay
US5039219A (en) * 1989-05-26 1991-08-13 Photon Technology Luminescence system and method for determining the nature of substances by measuring fluorescence and phosphorescence properties
US5252834A (en) * 1990-11-13 1993-10-12 Union Oil Company Of California Pulsed and gated multi-mode microspectrophotometry device and method
US5871906A (en) * 1991-11-08 1999-02-16 The Research Foundation Of State University Of New York Method for detection of amplified nucleic acid products and related diagnostic assays
DE4210970C2 (de) * 1992-04-02 1996-10-17 Markus Dipl Chem Sauer Verfahren zur simultanen optischen qualitativen und quantitativen Erfassung von verschiedenen mit Fluorochromen oder Fluorogenen markierten Molekülen eines Gemisches mittels Laserspektroskopie
US5418371A (en) * 1993-02-01 1995-05-23 Aslund; Nils R. D. Apparatus for quantitative imaging of multiple fluorophores using dual detectors
AU673245B2 (en) * 1993-02-01 1996-10-31 Seq, Ltd. Methods and apparatus for DNA sequencing
US5324401A (en) * 1993-02-05 1994-06-28 Iowa State University Research Foundation, Inc. Multiplexed fluorescence detector system for capillary electrophoresis

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6435268A (en) * 1987-06-16 1989-02-06 Wallac Oy Assay of biologically specific multiple object to be analyzed
JPH01227948A (ja) * 1988-03-08 1989-09-12 Japan Spectroscopic Co マルチチャンネル蛍光減衰波形測定装置
JPH02234050A (ja) * 1989-03-08 1990-09-17 Hamamatsu Photonics Kk 光波形測定装置
JPH05126739A (ja) * 1991-10-31 1993-05-21 Hamamatsu Photonics Kk 核酸塩基識別法
JPH07505467A (ja) * 1992-03-23 1995-06-15 ダイアトロン・コーポレイション フルオロメーター検出システム
JPH06148076A (ja) * 1992-11-10 1994-05-27 Hamamatsu Photonics Kk 核酸の塩基配列決定方法、単一分子検出方法、その装置及び試料の作成方法

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2012042467A (ja) * 2010-08-13 2012-03-01 Leica Microsystems Cms Gmbh 光学装置の光路において検知信号を分離する方法
US9651765B2 (en) 2010-08-13 2017-05-16 Leica Microsystems Cms Gmbh Method for separating detection signals in the beam path of an optical device

Also Published As

Publication number Publication date
WO1998009154A1 (de) 1998-03-05
DE19634873A1 (de) 1998-03-12
EP0857300A1 (de) 1998-08-12
US6140048A (en) 2000-10-31

Similar Documents

Publication Publication Date Title
JP2000500874A (ja) 時間分解蛍光測定を使用する蛍光分子群識別のためのシステム
US20160252456A1 (en) Single particle detection device, single particle detection method, and computer program for single particle detection, using optical analysis
JP3448090B2 (ja) エネルギー移動検出法およびその装置
JP5802653B2 (ja) 光分析装置、光分析方法並びに光分析用コンピュータプログラム
JP3830167B2 (ja) 試料媒体にある標的粒子の所定の特性を決定するための方法および装置
US7906768B2 (en) Imaging of biological samples
JP5893564B2 (ja) 複数の波長帯域の光計測を用いた光分析方法
JP4776854B2 (ja) 蛍光粒子を有する試料を特徴付ける方法
US6384914B1 (en) Method for optical detection of analyte molecules in a natural biological medium
WO1996027798A1 (en) A biospecific assay method
EP0804732A1 (en) A biospecific multiparameter assay method
CN104115001B (zh) 利用单个粒子检测的光分析装置、光分析方法
US20040023229A1 (en) Direct detection of individual molecules
EP2743684B1 (en) Method for detecting fluorescent particles
WO2003038413A1 (en) Apparatus for analyzing florescent image of biochip
EP1102990B1 (en) Sample analysis
WO2005080945A9 (en) Method for avoiding bleaching when using fluorescence correlation spectroscopy
FI96641B (fi) Biospesifinen määritysmenetelmä
JP2009150665A (ja) 一分子蛍光・りん光分析方法及びそのための装置
Keller et al. Analytical Applications
JP2011013143A (ja) 濃度測定方法及び濃度測定装置

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20040629

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20040629

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20050816

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20051116

A602 Written permission of extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A602

Effective date: 20060106

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20060516

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20081224