JP2000510334A - 原核生物ｒｎａポリメラーゼ自己遺伝子を利用する細胞質遺伝子発現系 - Google Patents

原核生物ｒｎａポリメラーゼ自己遺伝子を利用する細胞質遺伝子発現系

Info

Publication number
JP2000510334A
JP2000510334A JP09539094A JP53909497A JP2000510334A JP 2000510334 A JP2000510334 A JP 2000510334A JP 09539094 A JP09539094 A JP 09539094A JP 53909497 A JP53909497 A JP 53909497A JP 2000510334 A JP2000510334 A JP 2000510334A
Authority
JP
Japan
Prior art keywords
sequence
cat
promoter
self
rna polymerase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP09539094A
Other languages
English (en)
Japanese (ja)
Inventor
ファン，リーフ
ロビンズ，ポウル
ジェフルス，ダニエル
ブリソン，マーニ
リー，ソン
ヤン，ジンピン
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Pittsburgh
Original Assignee
University of Pittsburgh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Pittsburgh filed Critical University of Pittsburgh
Publication of JP2000510334A publication Critical patent/JP2000510334A/ja
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/88—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microencapsulation, e.g. using amphiphile liposome vesicle
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C12N2800/108—Plasmid DNA episomal vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES
    • C12N2840/206—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES having multiple IRES

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP09539094A 1996-04-26 1997-04-25 原核生物ｒｎａポリメラーゼ自己遺伝子を利用する細胞質遺伝子発現系 Pending JP2000510334A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US1630496P 1996-04-26 1996-04-26
US60/016,304 1996-04-26
PCT/US1997/007030 WO1997041243A2 (fr) 1996-04-26 1997-04-25 Systeme d'expression cytoplasmique de gene mettant en application un autogene procaryote d'arn polymerase

Publications (1)

Publication Number Publication Date
JP2000510334A true JP2000510334A (ja) 2000-08-15

Family

ID=21776448

Family Applications (1)

Application Number Title Priority Date Filing Date
JP09539094A Pending JP2000510334A (ja) 1996-04-26 1997-04-25 原核生物ｒｎａポリメラーゼ自己遺伝子を利用する細胞質遺伝子発現系

Country Status (5)

Country Link
EP (1) EP0910653A2 (fr)
JP (1) JP2000510334A (fr)
AU (1) AU2744697A (fr)
CA (1) CA2250759A1 (fr)
WO (1) WO1997041243A2 (fr)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU763325B2 (en) 1998-01-15 2003-07-17 King's College London Ribozymal nucleic acids cleaving CCR5 or CXCR4
US6342596B1 (en) * 1998-05-05 2002-01-29 Hsf Pharmaceuticals S.A. Molecular regulatory circuits to achieve sustained activation of genes of interest by a single stress
US20040142892A1 (en) * 2001-04-30 2004-07-22 The University Of British Columbia Autogene nucleic acids encoding a secretable RNA polymerase
WO2002088370A2 (fr) * 2001-04-30 2002-11-07 Protiva Biotherapeutics Inc. Acides nucleiques autogenes codants pour une polymerase d'arn secretable
GB0406330D0 (en) * 2004-03-20 2004-04-21 Univ Sheffield Gene screen
AU2016256579B2 (en) 2015-04-30 2021-08-12 Engenes Biotech Gmbh Uncoupling growth and protein production

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69022165T2 (de) * 1989-10-31 1996-04-04 Sagami Chem Res Klonierungs-Plasmid-Vektor, Primer-Vektor davon und Herstellungsverfahren einer cDNA-Bank.
AU652566B2 (en) * 1990-06-18 1994-09-01 United States of America, as represented by the Secretary, U.S. Department of Commerce, The Eukaryotic expression vector system
US5591601A (en) * 1993-05-14 1997-01-07 Ohio University Edison Animal Biotechnology Institute DNA polymerase gene expression system utilizing an RNA polymerase co-delivered with the gene expression vector system
CA2140081C (fr) * 1994-01-13 2008-04-01 Dean L. Engelhardt Procede, construct et conjugue permettant de produire des copies multiples d'acides nucleiques

Also Published As

Publication number Publication date
CA2250759A1 (fr) 1997-11-06
WO1997041243A3 (fr) 1998-02-19
EP0910653A2 (fr) 1999-04-28
WO1997041243A2 (fr) 1997-11-06
AU2744697A (en) 1997-11-19

Similar Documents

Publication Publication Date Title
Guan et al. Nanotechnologies in delivery of mRNA therapeutics using nonviral vector-based delivery systems
CN110714015B (zh) 一种mRNA狂犬病疫苗
Garnett Gene-delivery systems using cationic polymers
Tanimoto et al. No enhancement of nuclear entry by direct conjugation of a nuclear localization signal peptide to linearized DNA
JP6608284B2 (ja) 癌の治療に使用されるｒｎａトランススプライシング分子（ｒｔｍ）
JP2005529959A (ja) 細胞へのポリヌクレオチドの伝達をするための新規な方法
JP2002502243A (ja) トランスフェクション活性を有するインテグリン−ターゲッティングベクター
WO2005035764A1 (fr) Acides nucleiques autogenes codant un arn polymerase pouvant etre secrete
Yamada et al. Validation of the use of an artificial mitochondrial reporter DNA vector containing a Cytomegalovirus promoter for mitochondrial transgene expression
JP2021519101A (ja) ドナーｄｎａを連結するための改変型核酸編集システム
CA2325744A1 (fr) Fusion de liposome et vehicule d'administration
JP2011504375A (ja) 細胞の中に核酸を導入する医薬組成物及びその方法
JP2000510334A (ja) 原核生物ｒｎａポリメラーゼ自己遺伝子を利用する細胞質遺伝子発現系
Li et al. Delivery of a PCR amplified DNA fragment into cells: a model for using synthetic genes for gene therapy
JP2002508956A (ja) 遺伝子送達のための方法および組成物
WO1997041243A9 (fr) Systeme d'expression cytoplasmique de gene mettant en application un autogene procaryote d'arn polymerase
JP2022173834A (ja) 人工ウイルスキャプシド
JP2025520111A (ja) Ｒｎａアジュバント、その方法及び使用
CN120051568A (zh) 具有改善的活性的经修饰转运rna
JP2003534804A (ja) トランスフェクションの改良方法
WO2005054486A1 (fr) Procede de preparation de reactif de transfert genetique
EP1210122A1 (fr) Expression de gene au moyen de polynucleotides modifies de maniere covalente
JP2003504320A (ja) 生物学的材料の送達系
US20250034548A1 (en) Improved modular prime editing with modified effectors and templates
KR102515464B1 (ko) 핵산 또는 유전자 형질감염 효율을 개선하는 세포독성 완화 막 투과 펩타이드 및 이의 용도