JP2004242533A5 - - Google Patents
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- JP2004242533A5 JP2004242533A5 JP2003033932A JP2003033932A JP2004242533A5 JP 2004242533 A5 JP2004242533 A5 JP 2004242533A5 JP 2003033932 A JP2003033932 A JP 2003033932A JP 2003033932 A JP2003033932 A JP 2003033932A JP 2004242533 A5 JP2004242533 A5 JP 2004242533A5
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- JP
- Japan
- Prior art keywords
- ethylenediamine
- amino acid
- seq
- residue
- protein
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Description
【特許請求の範囲】
【特許請求の範囲】
【請求項１】 配列番号1記載のアミノ酸配列から成り、且つ、エチレンジアミン-N,N'-ジコハク酸：エチレンジアミンリアーゼ活性を有するタンパク質。
【請求項２】 配列番号１記載のアミノ酸配列において、120番目のリジン残基、166番目のイソロイシン残基及び365番目のアラニン残基のうち少なくとも1つのアミノ酸残基が他のアミノ酸残基に置換されたアミノ酸配列から成り、且つ、エチレンジアミン-N,N'-ジコハク酸：エチレンジアミンリアーゼ活性を有するタンパク質。
【請求項３】 請求項1に記載されたタンパク質をコードする遺伝子DNA。
【請求項４】 配列番号２記載の塩基配列から成る遺伝子DNA。
【請求項５】 請求項２に記載されたタンパク質をコードする遺伝子DNA。
【請求項６】 配列番号２記載の塩基配列において、358番目から360番目、496番目から498番目及び1093番目から1095番目の塩基のうち少なくとも1つの塩基が他の異なる塩基に置換された塩基配列から成る請求項５記載の遺伝子DNA。
【請求項７】 請求項３〜６いずれか１項に記載の遺伝子DNAをベクターDNAに挿入した組換えDNA。
【請求項８】 請求項７記載の組換えDNAを含む形質転換体又は形質導入体。
【請求項９】 請求項８記載の形質転換体又は形質導入体を培養し、得られる培養物からエチレンジアミン-N,N'-ジコハク酸：エチレンジアミンリアーゼを採取することを特徴とするエチレンジアミン-N,N'-ジコハク酸：エチレンジアミンリアーゼの製造方法。
【請求項１０】 フマル酸とジアミンとを、請求項８記載の形質転換体又は形質導入体の存在下で反応させ、得られる反応産物からジアミノアルキレン-N,N'-ジコハク酸を採取することを特徴とするジアミノアルキレン-N,N'-ジコハク酸の製造方法。
[Claims]
[Claims]
1. A protein comprising the amino acid sequence of SEQ ID NO: 1 and having ethylenediamine-N, N'-disuccinic acid: ethylenediamine lyase activity.
(2) In the amino acid sequence of SEQ ID NO: 1, an amino acid sequence in which at least one amino acid residue among the lysine residue at position 120, the isoleucine residue at position 166, and the alanine residue at position 365 has been replaced with another amino acid residue And a protein having ethylenediamine-N, N'-disuccinic acid: ethylenediamine lyase activity.
(3) A gene DNA encoding the protein according to claim 1.
(4) Consists of the nucleotide sequence of SEQ ID NO: 2Gene DNA.
(5) Claim 2A gene DNA encoding the protein described in (1).
6. In the nucleotide sequence of SEQ ID NO: 2, the nucleotide sequence is composed of a nucleotide sequence in which at least one of the 358th to 360th, 496th to 498th, and 1093th to 1095th bases has been substituted with another different base.Claim 5The described genetic DNA.
7. Claims 3-6A recombinant DNA wherein the gene DNA according to any one of the above is inserted into a vector DNA.
Claim 8. Claim 7A transformant or a transductant containing the recombinant DNA according to the above.
9. Claim 8Culturing the transformant or transductant as described above, and collecting ethylenediamine-N, N'-disuccinic acid: ethylenediamine lyase from the resulting culture, wherein ethylenediamine-N, N'-disuccinic acid: ethylenediamine lyase is collected. Manufacturing method.
10. Fumaric acid and diamine,Claim 8Reaction in the presence of the described transformant or transductant, diaminoalkylene-N, N'-disuccinic acid characterized by collecting the diaminoalkylene-N, N'- disuccinic acid from the resulting reaction product Production method.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2003033932A JP4253195B2 (en) | 2003-02-12 | 2003-02-12 | Modified ethylenediamine-N, N'-disuccinic acid: ethylenediamine lyase |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2003033932A JP4253195B2 (en) | 2003-02-12 | 2003-02-12 | Modified ethylenediamine-N, N'-disuccinic acid: ethylenediamine lyase |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2004242533A JP2004242533A (en) | 2004-09-02 |
| JP2004242533A5 true JP2004242533A5 (en) | 2006-03-30 |
| JP4253195B2 JP4253195B2 (en) | 2009-04-08 |
Family
ID=33019768
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2003033932A Expired - Fee Related JP4253195B2 (en) | 2003-02-12 | 2003-02-12 | Modified ethylenediamine-N, N'-disuccinic acid: ethylenediamine lyase |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP4253195B2 (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2007089513A (en) * | 2005-09-29 | 2007-04-12 | Nippon Shokubai Co Ltd | Novel acid-resistant modified S-hydroxynitrile lyase |
| EP2078748A4 (en) * | 2006-09-29 | 2010-01-06 | Nippon Catalytic Chem Ind | Novel highly active modified type s-hydroxynitrile lyase |
| JP7362999B2 (en) * | 2019-03-29 | 2023-10-18 | 三井化学株式会社 | Method for producing killed microorganisms, method for producing S,S-ethylenediamine-N,N'-disuccinic acid or its salt, and bacterial cell fluid |
| EP4556568A1 (en) | 2023-11-17 | 2025-05-21 | Basf Se | Biocatalytical methods for producing ethylenediamine-n,n'-disuccinic acid |
-
2003
- 2003-02-12 JP JP2003033932A patent/JP4253195B2/en not_active Expired - Fee Related
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