JP2005009065A - Antibacterial and bactericidal fiber products and methods for producing the same - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a textile product containing a material having excellent antibacterial and bactericidal properties against bacterial cells.
An antibacterial / bactericidal fiber product having the gist of including a fiber containing a photosensitizing dye as a constituent material, and in particular, the fiber containing a photosensitizing dye contains a cationizing agent and / or a sensitizing agent. Those treated with a sticking agent are preferred, and the photosensitizing dye is preferably rose bengal or phthalocyanine blue, and the cationizing agent is preferably a quaternary ammonium salt or tannic acid, and more preferably a dye. The fiber containing the sensitizing dye is preferably a fiber mainly composed of polyamide, and examples of the product form of the textile product include a mask or a cloth personal article, and a medical clothing or cloth personal article. It is also useful as a product.
[Selection figure] None

Description

  The present invention relates to a textile product having antibacterial and bactericidal properties. More specifically, the present invention relates to microorganisms such as viruses, bacteria, and fungi that cause infection such as droplet infection and air infection (hereinafter abbreviated as cells). It is an invention relating to a textile product including a material having antibacterial and bactericidal properties.

  Oral infection is known as one of the infection routes of bacterial cells such as viruses, bacteria, and fungi. As a means for preventing oral infection, a mask is generally used. By increasing the filter performance (particle collection rate) of the mask, it is possible to prevent the entry of bacterial cells from the outside. However, if the usage time is prolonged, the cells are concentrated on the filter.If the mask body is touched when the mask is detached, the cells adhere to the hand, and when the hand touches the mouth when eating or drinking, There arises a problem that the adherent cells are taken into the body.

  Such a problem is not limited to masks, but if the bacterial cells are attached to personal belongings such as clothes, towels, handkerchiefs, etc., the bacterial cells will adhere to the hand when touching the splashed part A similar problem arises.

  In view of these problems, for example, a technique for preventing the growth of bacterial cells attached to a mask has been proposed.

For example, Patent Document 1 proposes a mask provided with a filter carrying a biguanite antibacterial agent. Patent Document 2 proposes a mask provided with a filter in which polymer fine particles containing a fine powder of antibacterial agent and quartz andesite obtained by pearl polymerization are fixed with a binder. Further, Patent Document 3 proposes a mask having a filter on which silver is deposited.
JP 2002-65879 A JP 2001-245997 A JP-A-11-19238

  This invention is made | formed in view of the said problem, The objective is to provide the textiles containing the raw material which has the outstanding antibacterial and bactericidal property with respect to a microbial cell.

  The present invention that has solved the above-mentioned problems is an antibacterial and bactericidal fiber product characterized by containing a fiber containing a photosensitizing dye as a constituent material.

  In particular, the fiber containing a photosensitizing dye is preferably treated with a cationizing agent and / or a thickening agent.

  Preferred as the photosensitizing dye is rose bengal or phthalocyanine blue, and the cationizing agent is preferably a quaternary ammonium salt or tannic acid.

  The fiber containing the photosensitizing dye is preferably treated with a dyeing accelerator such as polyphenols during dyeing.

  The fiber containing the photosensitizing dye of the present invention is preferably a fiber mainly composed of polyamide, and examples of the product form of the fiber product include a mask or a cloth personal article, and a medical clothing It is also useful as a personal belongings made of cloth.

  According to the present invention, the microbial cells can be sterilized by exciting the photosensitizing dye present in the fiber, so that the microbial cells can be prevented from growing or contacting. Even when no excitation action occurs in a dark place or the like, the cells attached to the mask can be sterilized by applying light to the mask thereafter.

  In addition, when a dye enhancer is used during dyeing of the photosensitizing dye, the dyeing property of the photosensitizing dye can be improved. In particular, when polyphenols are used as dyeing accelerators, the antibacterial properties of polyphenols are also exhibited.

  Further, the bactericidal action by the photosensitizing dye is further improved by treating the fiber containing the photosensitizing dye with a cationizing agent.

  Further, when a pressure-sensitive adhesive is applied to a fiber containing a photosensitizing dye, preferably a fiber product containing the fiber as a constituent material, the bactericidal action is further enhanced by improving the capture and retention of cells.

  Further, as described above, since the photosensitizing dye can be imparted to the fiber by a simple method, it can be produced at a lower cost than other antibacterial fibers that require vapor deposition treatment, such as titanium oxide. Moreover, according to the immersion treatment, the photosensitizing dye can be uniformly applied to the fiber by a simpler method than the vapor deposition treatment.

  As a result of the present inventors' extensive research on antibacterial and bactericidal fiber products, fiber products containing photosensitizing dye-containing fibers as constituent materials are extremely superior against cells such as viruses, bacteria, and fungi. The present invention has been found to exhibit antibacterial and bactericidal properties.

  When light is applied to the photosensitizing dye, the photosensitizing dye is excited, and singlet oxygen is generated from oxygen (triplet oxygen) in the air by the excitation energy (hereinafter, sometimes referred to as “excitation action”). ). This singlet oxygen is active oxygen, and the oxidizing action of the singlet oxygen makes it possible to sterilize the cells, destroy the cells, and prevent the growth of the cells (antibacterial / bactericidal action).

  In the present invention, the photosensitizing dye refers to a dye that can generate singlet oxygen by an excitation action by light. The photosensitizing dye having such an action is not particularly limited. For example, rose bengal, methylene blue, phthalocyanine dye, eosin, tetraphenylporphyrin, rubrene, aromatic polycyclic hydrocarbon compound, 7,12-dimethylbenzo Anthracene etc. are mentioned. Among these, rose bengal and phthalocyanine blue that exhibit high excitation action even under low light (for example, about 15 to 45 lux) are recommended. Assuming that the textile product of the present invention is used in a part that comes into contact with the human body such as clothes or a mask, it is desirable to use a photosensitizing dye that does not affect the human body even if it is eluted from the fiber. . From such a viewpoint, a photosensitizing dye that is medically allowed to be ingested is preferable, and, for example, rose bengal is recommended. From the viewpoint of dissolution resistance, phthalocyanine blue is preferable because it is less likely to elute than rose bengal.

  The fiber containing the photosensitizing dye is not particularly limited, and various known fibers can be used. For example, natural fibers such as cotton, hemp, wool, silk, and polylactic acid fibers; chemical fibers such as acetate, acrylic, cupra, polyamide, vinylon, polypropylene, polyester, polyurethane, polyvinyl chloride, vinylidene, and rayon (synthetic fibers, semi-synthetic) And various composite fibers such as polyethylene-polyester composite fibers and polyethylene-polypropylene composite fibers. These fibers may be used alone or in combination of two or more. Of these, polyamide (especially nylon) excellent in dyeability and retention (elution resistance) with respect to the photosensitizing dye is particularly preferable. In addition, what is necessary is just to select suitably fiber forms, such as a cross section of a fiber, the shape of a side surface, length, and thickness, according to a use.

  In the present invention, the presence form of the photosensitizing dye is not particularly limited. For example, the photosensitizing dye may be attached to the fiber surface or may be included in the fiber. The amount of the photosensitizing dye contained in the fiber is not particularly limited as long as the fiber is colored with the photosensitizing dye. Note that the photosensitizing dye exhibits an excitation action regardless of the quantity.

  In order to excite the photosensitizing dye, light having a wavelength capable of exciting the photosensitizing dye may be irradiated. The specific wavelength varies slightly depending on the type of photosensitizing dye, but in the range of near ultraviolet light to visible light (about 240 nm to 700 nm), the photosensitizing dye can be excited to generate singlet oxygen. Light from sunlight or fluorescent light may be applied to the photosensitizing dye.

  The method for applying the photosensitizing dye to the fiber is not particularly limited, and various known dyeing methods (for example, immersion, coating, etc.) may be employed.

  For example, in the case of immersion treatment, the fibers may be immersed in a dye bath containing a photosensitizing dye and then dried. At this time, the temperature of the dyeing bath, the pH of the dyeing solution, the ratio of the dyeing solution to the fiber product, the dyeing time, the dyeing conditions such as the dyeing assistant may be appropriately selected according to the properties of the fiber and the required properties, preferably When the dye bath temperature is adjusted to about 100 to 130 ° C. and left standing in the dye bath for a certain time (for example, about 10 minutes to 2 hours), sufficient dyeability can be obtained. Especially in the case of chemical fibers such as polyamide, if the fiber surface is swollen at the time of dyeing, the photosensitizing dye can be taken into the fiber, so that elution of the photosensitizing dye during use is suppressed and the durability life is increased. Can do. Either immersion or post-dying can be used for the immersion treatment.

  If a photosensitizing dye is present, the bactericidal action is exhibited, and therefore, a printing process may be employed as a dyeing method to dye only an arbitrary portion. Even if the photosensitizing dye is present only in a part of the fiber, a sufficient bactericidal action is exhibited in the existing part of the photosensitizing dye.

  In the case of chemical fiber, the fiber may be produced using a fiber raw material containing a photosensitizing dye. For example, when producing a polyamide fiber containing a photosensitizing dye, the fiber may be dipped as described above, and the photosensitizing dye may be attached to the fiber or penetrated into the fiber. Spinning and drawing using fiber materials containing photosensitizing dyes in advance, such as fiber materials obtained by adding and kneading fiber materials and fiber materials obtained by immersing the chips in a dye-containing dye-containing bath You may manufacture the polyamide fiber containing a photosensitizing dye by giving a post-process.

  When dyeing, a dyeing accelerator may be used to enhance the dyeability of the photosensitizing dye to the fiber. In particular, when a polyphenol reagent is used as a dyeing accelerator, in addition to the antibacterial and bactericidal action of the photosensitizing dye, the antibacterial property of the polyphenol itself is imparted, so that the antibacterial property of the fiber is further improved, which is desirable. In addition, it is preferable to use polyphenols extracted from natural raw materials such as coffee beans because the elution of polyphenols can also prevent the human body from being affected. Moreover, it is desirable to use a dyeing accelerator having antibacterial properties, such as polyphenols, since the antibacterial properties are exhibited even in a dark place where the photosensitizing dye is difficult to be excited, and the growth of bacterial cells can be suppressed.

  Examples of polyphenols include phenol and cresol in addition to the coffee bean extract. Besides polyphenols, for example, inorganic acids such as sulfuric acid, hydrochloric acid, nitric acid, lime acid, citric acid, oxoglutaric acid, succinic acid, fumaric acid, malic acid, itaconic acid, gluconic acid, ketogluconic acid, ascorbic acid, acetic acid, Organic acids such as formic acid, lactic acid, carboxylic acid, cumic acid, salicylic acid, hydrogen peroxide, and the like can also be used as a dyeing accelerator.

  As described above, it may be processed into a knitted fabric, a woven fabric, a felt, a non-woven fabric, or the like by using a fiber preliminarily containing a photosensitizing dye by a known dyeing method, or the fiber may be processed into a knitted fabric, a woven fabric, a felt, a non-woven fabric, or the like. You may give a photosensitizing dye after processing. In the case of a chemical fiber, spinning may be performed using a fiber raw material containing a photosensitizing dye, and the processing may be performed using the obtained fiber.

  In the present invention, it is recommended to further treat the fiber containing the photosensitizing dye with a cationizing agent. This is because the presence of a cationizing agent together with the photosensitizing dye further enhances the bactericidal effect. This is thought to be because, generally, the surface layer of the microbial cell is anion-charged, and the microbial cell is attracted to the effective sterilization range of singlet oxygen by the cationizing agent. The kind of the cationizing agent is not particularly limited as long as it can cationize the fiber. Chitosan, tannic acid, tartaric acid, salicylic acid, quaternary reactive compound (particularly quaternary ammonium compound), polyethylene Examples include polyamine cationic agents or mixtures or reactants thereof. Of these, tannic acid, chitosan, salicylic acid, and quaternary reactive compounds themselves have antibacterial properties and exhibit antibacterial effects even in the dark as described above. Tannic acid, chitosan, and salicylic acid are preferable because they do not adversely affect the human body even if they are eluted.

  The quaternary reactive compound is preferably a quaternary ammonium compound (an ionic compound containing a positive ammonium group formed by binding four alkyl or aryl groups to a nitrogen atom), more preferably a quaternary ammonium compound. It is salt. Quaternary ammonium salts (for example, benzalkonium chloride and benzethonium chloride in particular) have excellent bactericidal properties as used as disinfectants in the medical field, and have a synergistic effect with the bactericidal action of singlet oxygen. Is desirable. Examples of quaternary ammonium salts include benzalkonium chloride, benzethonium chloride, stearyldimethylbenzylammonium chloride, stearyltrimethylammonium chloride, stearylpentaethoxyammonium chloride, methylbenzethonium chloride, cetylpyridinium chloride, alkylisoquinolinium bromide, odor Domifene, dimethyliminopolyethylene chloride, dimethyldimethylammonium chloride, 3-chloro-2-hydroxypropyltrimethylammonium chloride, 2,3-epoxypropyltrimethylammonium chloride, or quaternary as exemplified in [Chemical Formula 1] below An ammonium salt type water-soluble resin is exemplified.

  The addition of tannic acid is desirable because it exerts an effect of agglutinating the virus by the protein denaturing effect or adsorbing it onto the surface of tannic acid, and thus exhibits a superior antibacterial and bactericidal action.

  In the present invention, the method for applying the cationizing agent is not particularly limited, and any method such as immersing the fiber in the cationizing agent-containing solution or applying the solution to the fiber can be adopted. In particular, when the fiber is treated with a cationizing agent after the photosensitizing dye is applied to the fiber, the photosensitizing dye is coated with the cationizing agent. Will improve. In addition, if a cationizing agent is present outside the photosensitizing dye, the cationizing agent can easily attract and capture cells within the effective singlet oxygen reach, thereby enhancing the antibacterial and bactericidal effects due to excitation. This is desirable.

  In addition, since the said effect | action of a cationizing agent is acquired also in the dark place where an excitation effect | action does not arise, a microbial cell can be capture | acquired and hold | maintained by the effect | action of a cationizing agent also in a dark place.

  In the present invention, the processing form of the above-described fiber containing a photosensitizing dye (or a fiber to be provided with a photosensitizing dye) is not particularly limited. For example, it can be processed into a desired textile product such as a woven fabric, a knitted fabric, a nonwoven fabric, or a felt using a fiber containing a photosensitizing dye. Moreover, you may give various additives according to uses, such as a flame retardant, a ultraviolet absorber, and a weather resistance agent, to the textiles obtained in this way as needed. Moreover, when manufacturing the mask which contains the fiber containing this photosensitizing dye as a constituent material, it is also promising to coat the fiber containing this photosensitizing dye with thickeners, such as agar. Coating with a thickener is desirable because the cells can be adhered and retained, so that scattering of the cells can be suppressed.

  The method for applying the thickener is not particularly limited, and the fiber containing the photosensitizing dye may be immersed in the thickener-containing liquid. In addition, the adhesive force of a fiber can be adjusted by controlling the amount of thickeners in a thickener containing liquid. Usually, even if it has very weak adhesive force, it can fully exhibit microbial cell adhesive force, Therefore Thickener concentration in a thickener containing liquid may be about 0.01-3 weight%, for example.

  Thickeners include seaweed extracts such as agar, carrageenan, alginic acid, and far celeran; seed mucous substances such as locust bean gum, guar gum, syrup gum, tamarind gum; resin extracts such as gum arabic, tragacanth gum, karaya gum; pectin, arabino Synthetic thickeners such as natural polysaccharides (thickening polysaccharides), polyethylene oxide, carboxyvinyl polymer, etc., which are made from plant mucous substances such as galactan; starch; do it. In particular, natural polysaccharides are desirable because they do not adversely affect the human body even if they are eluted.

  In order to give such a thickener, the thickener may be attached to the fiber before being processed into a fiber product. Since it may cause problems such as the processing machine being hindered by the adhesive, coat the thickener by, for example, immersing the textile in a thickener-containing liquid after processing into the desired textile. Is desirable.

  What is necessary is just to perform the process by the said dyeing | staining promoter, a cationizing agent, and a thickener arbitrarily combining.

  The amount of the photosensitizing dye contained in the fiber is not particularly limited, but it is desirable to increase the amount of the photosensitizing dye in order to maintain the effect of the photosensitizing dye. In order to maximize the excitation effect, it is desirable that the entire fiber product is composed of a fiber containing a photosensitizing dye, but the fiber product contains the fiber containing the photosensitizing dye as a constituent material. The fiber product need not be composed of only the fibers containing the photosensitizing dye. That is, if a fiber containing a photosensitizing dye is contained at a desired position of the fiber product, the bactericidal action is exhibited by the fiber. Of course, the fiber product may be composed only of fibers containing a photosensitizing dye. For example, in the case of a mask, a gauze manufactured with a fiber containing a photosensitizing dye is included as a constituent of the mask in a mask manufactured using a normal fiber (a fiber not containing a photosensitizing dye) (sandwich method or the like). In some cases, the antibacterial and bactericidal properties are excellent at the gauze portion. Moreover, you may manufacture a mask using the fiber containing a photosensitizing dye.

  Examples of the textile products include masks, cloth accessories, medical clothing, and medical cloth accessories from the viewpoint of preventing infection by bacteria, but not limited to these, the photosensitization of the present invention is applied to all textile products. It is possible to provide a fiber product using a fiber containing a pigment as a constituent material.

  Examples of the mask include general commercial products and medical masks [DS1, DS2, DS3 (national test standards)].

Examples of cloth items include handkerchiefs, towels, ties, wipes of glasses, cloth products that touch directly with your hands, such as rags, cloths, dust birds, and handle handles of straw;
Examples of medical clothing include fabric products mainly used for medical purposes such as gowns, aprons, surgical clothes, lab coats, and shoe covers;
Examples of medical personal items include caps, sheets, pillow covers, bandages, mops, gauze, masks, filters, shoes, gloves, covering cloths, drapes, water absorbent cloths, and other cloth products used in the medical field;
Textile products other than the above include clothing, underwear, lining, shirts, blouses, training pants, work clothes, towels, sheets, scarves, socks, stockings, sweaters, footwear, supporters and other clothing products, curtains, futons, carpets, Examples include furniture covers, headrest covers, blankets, mattresses, and bedding products such as bedding. In addition, daily necessities such as mop are listed.

  Hereinafter, a mask will be described as a representative example of the textile product of the present invention with reference to the drawings. However, the following description is an embodiment and can be appropriately changed.

  FIG. 1 shows an example of a mask which is one product form of the textile product of the present invention. In the figure, reference numeral 1 denotes a mask main body to which an ear string 2 is attached. The mask main body 1 includes an outermost gauze 1a, an innermost gauze 1b (human body contact side), a particulate trapping filter 3, and a fiber containing a photosensitizing dye. It is comprised with the sterilization filter 4 comprised by these.

  The sterilizing filter 4 is obtained by dipping a gauze (cotton) manufactured in advance and including a photosensitizing dye. Of course, the filter may be made of the above-described chemical fibers such as polyester and polyamide in addition to natural fibers such as cotton.

  In the dipping treatment, the gauze is dipped in a dye bath containing a photosensitizing dye (rose bengal) and a dyeing accelerator (polyphenol extracted from coffee) to color the gauze, dried, and then cationized to the gauze. After dipping and drying in an agent (tannic acid) -containing solution, the product is immersed in a natural thickener (agar) -containing solution and an adhesive is attached to the fiber outer surface of the gauze.

  The mask body 1 has a structure mainly composed of a plurality of gauze (1a, 1b). However, the structure of the mask body 1 is not particularly limited, and may be formed of an arbitrary number of sheet-like cloths. Any of woven fabric, knitted fabric, non-woven fabric and the like can be used. Further, the mask main body 1 may be formed in an arbitrary shape in combination with a three-dimensional face cushion. Although the gauze 1a, 1b constituting the mask body is not dyed with a photosensitizing dye, these gauze may be dyed with a photosensitizing dye as in the case of the sterilizing filter 4.

  The fine particle capture filter 3 may be a filter having a desired dust collection efficiency as required. The use of the filter 3 is optional, but in medical applications, it is preferable to use a filter having a dust collection efficiency that satisfies DS1, more preferably DS2, and even more preferably DS3 (national test standard). The fiber constituting the particulate trapping filter 3 may contain a photosensitizing dye in the fiber in the same manner as the sterilization filter 4.

  In the present invention, the antibacterial and bactericidal action is exerted by the sterilizing filter 4 manufactured by the fiber containing the photosensitizing dye, but if the mask contains the fiber containing the photosensitizing dye as a constituent material, The use of the sterilizing filter 3 is optional. For example, instead of the sterilizing filter 4, only the fine particle capturing filter 3 containing a photosensitizing dye may be used.

  It is preferable to manufacture the sterilizing filter 3 using a fiber containing a photosensitizing dye and replace only the filter 3 because the manufacturing cost and filter replacement cost can be reduced and the running cost can be reduced.

  The material of the ear strap 2 is not particularly limited, and a known material can be used. However, the ear strap is produced from the above-mentioned fiber, and a photosensitizing dye is contained in the fiber, thereby attaching bacteria on the ear strap 3.・ Can also prevent breeding.

  The mounting position of the sterilizing filter 3 is not particularly limited, and may be sandwiched at any position on the outermost surface of the mask (outside the gauze 1a), the innermost surface (outside of the gauze 1b), or the mask intermediate layer. In order to excite the photosensitizing dye, it is not necessary to directly irradiate the photosensitizing dye with light, and indirect light may be used. Therefore, even when the sterilizing filter 4 is sandwiched between the mask intermediate layers or disposed on the innermost surface side, the photosensitizing dye is excited by the light incident from the outermost surface side of the mask, and exhibits an excellent antibacterial and sterilizing effect.

  In order to prevent the sterilization filter 4 from being displaced from the mask body during use, it is desirable to insert the sterilization filter 4 as a mask intermediate layer and hold it as shown in the figure. Further, the insertion position as the mask intermediate layer is not particularly limited, and may be on the surface side or the inner surface side of the fine particle capturing filter 3. Further, a plurality of sterilizing filters 4 may be used together and arranged at a desired position on the mask. In addition, since the excitation effect | action of a higher photosensitizing dye can be acquired, so that a lux is high, the installation position of the fiber containing a photosensitizing dye is so desirable that it is near the mask outermost surface.

  Moreover, when attaching the sterilization filter 4 to the place which cannot be clamped, such as the outer surface side of the gauze 1a, it is also preferable to use an adhesive member such as a hook-and-loop fastener.

  As mentioned above, masks that contain fibers containing photosensitizing dyes as constituent materials exhibit the antibacterial and bactericidal action of photosensitizing dyes, so that the bacteria attached to the mask adhere to the hand Can be solved.

  In particular, when a mask made of a fiber containing a photosensitizing dye is worn, the humidity of the mask body increases due to the moisture contained in exhaled breath during use, and the excitation action of the photosensitizing dye acts on the cells by the moisture. It becomes easier and exhibits better antibacterial and bactericidal action.

  As described above, the action of the present invention has been described using a mask as a representative example. However, other fiber products containing the photosensitizing dye of the present invention as a constituent material, and excellent antibacterial and bactericidal properties can be achieved with the same configuration. .

  As described above, since the fiber product containing the fiber containing the photosensitizing dye of the present invention as a constituent material has a bactericidal action, it is particularly a fiber product such as a mask, a cloth garment, a medical garment, and a medical cloth garment. Since it can sterilize the microbial cells adhering to the fibers, infection caused by the body touching the microbial cells adhering to the fibers can be suppressed as much as possible.

  Moreover, the textile product of the present invention exhibits a sufficient bactericidal action by singlet oxygen generated by light rays that are weaker than sunlight, such as indoor light such as fluorescent lamps or indirect light. Therefore, the fiber product of the present invention is useful because it provides an antibacterial and bactericidal action under normal use conditions.

Virus inactivation tests were performed on herpes virus Type 1 (hereinafter referred to as herpes virus) and swine infectious gastroenteritis virus (hereinafter referred to as coronavirus). Virus titer was determined using TCID ₅₀ .
Test Material and Method [Preparation of Fiber Containing Photosensitizing Dye]
A cotton cloth (gauze: 10 cm × 10 cm) was processed as follows to obtain a test cloth piece.
Test Cloth R: A cotton cloth is immersed in a dye bath (1% by weight Rose Bengal aqueous solution) containing a photosensitizing dye.
(105 ° C., 30 minutes), and the cloth was made to contain a photosensitizing dye.
Test piece R + T: Cotton cloth in a dye bath containing a photosensitizing dye (1% by weight Rose Bengal aqueous solution)
After dipping (105 ° C., 30 minutes) and adding a photosensitizing dye to the cloth,
After drying, the cloth is tannic acid-containing liquid (0.35 wt% tannic acid aqueous solution).
(105 ° C, 30 minutes) and coat the cloth with tannic acid.
I did.
Test Cloth N: Cotton Cloth [Control without photosensitizing dye and tannic acid]
[Virus growth and regulation]
Herpes virus was grown in Vero cells, and the virus titer was adjusted to 10 ⁶ units / ml by diluting with phosphate buffer.
-Coronavirus was grown in Vero cells, and the virus titer was diluted with phosphate buffer and adjusted to 10 ⁶ units / ml.
-Virus negative control sample: A phosphate buffer containing neither virus was prepared as a control.

[Virus inactivation test with singlet oxygen]
The following experiment was performed aseptically on a biohazard clean bench. Since the dyed test cloth piece may be attached not only to the virus but also airborne bacteria at the same time during use, the tool and dyed test piece used for the test were not sterilized. The supernatant (virus-containing portion) after the virus inactivation test using singlet oxygen was sterilized by filtration through a membrane filter (pore size: 0.2 μm), and the virus titer was measured.

Place herpes virus or coronavirus suspension (10 ⁶ pfu / ml, 10 mL) in an Erlenmeyer flask, and then put each test cloth piece into a 1 m distance under two 40 W fluorescent lamps (under visible light, light quantity is 600 ˜650 lux) and allowed to stand for 10 minutes and 30 minutes with stirring 20 times per minute. 3 mL of the virus suspension was collected every predetermined time, and sterilized by filtration with a membrane filter (pore size: 0.2 μm) to obtain a virus solution. This virus solution was diluted 10-fold with phosphate buffer, added 0.1 mL each to a Vero cell culture plate, and the virus was adsorbed to the cells in the presence of 5% CO ₂ in a room maintained at 37 ° C. for 1 hour. . After the adsorption reaction, the virus solution was removed, 1% FCS (fetal bovine serum) -containing DMEM medium (Dulbecco's modified Eagle medium) was added, and the room was maintained at 37 ° C. (in the presence of 5% CO ₂ ) for 5 days. Cultured. In the determination, virus growth was positive when the virus-infected cells were denatured, and virus growth was negative when no cell degeneration was observed.

[result]
[Results against herpes virus]
Test cloth R: 10 ⁶ herpesviruses were inactivated to 10 ¹ or less within 10 minutes, and the same level of inactivation was maintained after 30 minutes.
Test strip R + T: 10 ⁶ herpesviruses were inactivated to 10 ¹ or less within 10 minutes, and the same level of inactivation was maintained after 30 minutes.
Test specimen N: Herpes virus showed 10 ⁶ even 30 minutes after the start and further 2 hours later, and virus inactivation was not observed.
[Results against coronavirus]
Test strip R: 10 ³ coronaviruses were inactivated within 10 minutes and 10 ⁵ coronaviruses were inactivated within 30 minutes.
Specimen R + T: 10 ³ coronaviruses were inactivated within 10 minutes and 10 ⁵ coronaviruses were inactivated within 30 minutes.
-Specimen N: Coronavirus showed 10 ⁶ even 30 minutes after the start and further 2 hours later, and virus inactivation was not observed.

[Antimicrobial test]
The antibacterial property was determined according to the JIS L 2002 halo (bacterial growth inhibition circle) measurement method and the JIS L 2002 bacterial solution absorption method.

[Create test cloth]
A cotton cloth (gauze: 10 cm × 10 cm) was subjected to high-pressure steam sterilization and then subjected to the following treatment to obtain a test cloth piece.

  The test cloth piece (cotton gauze: 10 cm × 10 cm) is immersed (105 ° C., 30 minutes) in a dye bath (1 wt% rose bengal aqueous solution) containing a photosensitizing dye, and then washed once with excess dye. After being removed and dried, the cloth was dipped (105 ° C., 30 minutes) in a tannic acid-containing liquid (0.35 wt% tannic acid aqueous solution), and the cloth was coated with tannic acid. The antibacterial property was tested by the halo test method (diameter: about 20 mm) and the bacterial solution absorption test method (20 mm × 20 mm) using the obtained test cloth piece and the cloth piece (control cloth piece) that was not dyed.

[Hello test method]
Bacteria (Staphylococcus aureus, superficial staphylococci, Escherichia coli, Salmonella, Vibrio parahaemolyticus, Serratia, Pseudomonas aeruginosa, Enterococcus) and fungi (Candida albicans, Aspergillus niger) were used for the experiment. . Each strain was cultured overnight at 35 ° C. in an enrichment medium (heart infusion liquid medium) to prepare a bacterial solution, and then smeared on a normal agar plate medium so as to be 10 ⁵ to ⁶ cfu / ml. Since Vibrio parahaemolyticus is a halophilic bacterium, the salt concentration in the overnight culture medium and the agar plate medium was adjusted to 3% by weight. Prior to testing, the test and control fabrics were autoclaved. The test cloth piece (20 mm) and the control cloth piece (20 mm) were brought into direct contact with the center of the medium to which the bacterial solution was applied, respectively, and were kept for 48 hours in a room maintained at 35 ° C. under a fluorescent lamp (600 to 700 lux light was irradiated) Culture was performed. After the cultivation, the distance of the inhibition zone part of the bacteria formed on the agar plate medium was measured.

[result]
Test cloth: Although the diameter of the inhibition band slightly differs depending on the bacterial species, a inhibition band of 25 mm to 31 mm including the diameter of the test cloth was confirmed. Also, no colony formation was observed under the test cloth.

  Control cloth piece: No inhibition band was formed on the agar plate medium smeared with bacteria. In addition, a large number of colonies were observed immediately below the control cloth.

[Bacteria absorption test method]
Bacteria (Staphylococcus aureus, superficial staphylococci, Escherichia coli, Salmonella, Serratia, Pseudomonas aeruginosa, enterococci) and fungi (Candida albicans, Aspergillus niger) were used as strains. Each strain was cultured overnight at 35 ° C. in an enrichment medium (heart infusion liquid medium) to prepare a bacterial solution, and then smeared on a normal agar plate medium so as to be 10 ⁵ to ⁶ cfu / ml. Prior to testing, the test and control fabrics were autoclaved. 0.2 mL of the culture solution was inoculated into several parts of each of the test cloth and the control cloth, and the inoculated vial was maintained at 35 ° C. under a fluorescent lamp (irradiated with light of 600 to 700 lux). The stationary culture was performed indoors for 18 hours. After incubation, the test cloth pieces and control cloth pieces were washed out with 20 ml of physiological saline, and the number of viable bacteria in the physiological saline was measured. Table 1 shows the average of the measured values obtained.

  From the above test results, the test strip containing the photosensitizing dye and cationizing agent has excellent antibacterial properties against gram-positive and negative bacteria and fungi, and has a wide antibacterial spectrum. I understood.

  The fiber product containing the fiber containing the photosensitizing dye of the present invention as a constituent material exhibits an extremely excellent antibacterial and bactericidal action against bacterial cells (various microorganisms containing proteins as constituents) as exemplified below.

Protozoa: Amoeba, Amebic dysentery Fungus: Aspergillus, Cryptococcus, Candida albicans Bacteria: Nosocomial infection pathogens such as Pseudomonas aeruginosa, Staphylococcus aureus, MRSA, VRSA, VRSE, VRE, Serratia; O-157, Enterococcus Food poisoning bacteria such as Vibrio parahaemolyticus Rickettsia: Tsutsugamushirikuchia Chlamydia: Parrot disease Chlamydia virus: Coronavirus, SARS, herpes, AIDS virus, adenovirus, HBV, HCV

It is a general | schematic sketch which shows an example of the textiles of this invention.

Explanation of symbols

DESCRIPTION OF SYMBOLS 1 Mask main body 1a Mask outermost surface side gauze 1b Mask innermost surface side gauze 2 Ear string 3 Fine particle capture filter 4 Sterilization filter

Claims (10)

  An antibacterial and bactericidal fiber product characterized by containing a fiber containing a photosensitizing dye as a constituent material.   The antibacterial and bactericidal fiber product according to claim 1, wherein the fiber containing a photosensitizing dye is treated with a cationizing agent.   The antibacterial / bactericidal fiber product according to claim 1 or 2, wherein the fiber containing a photosensitizing dye is treated with a thickener.   The antibacterial and bactericidal fiber product according to any one of claims 1 to 3, wherein the photosensitizing dye is rose bengal or phthalocyanine blue.   The antibacterial and bactericidal fiber product according to any one of claims 2 to 4, wherein the cationizing agent is a quaternary ammonium salt or tannic acid.   The antibacterial / bactericidal fiber product according to any one of claims 1 to 5, wherein the fiber containing a photosensitizing dye is treated with a dyeing accelerator.   The antibacterial and bactericidal fiber product according to any one of claims 1 to 6, wherein the fiber containing a photosensitizing dye is a fiber mainly composed of polyamide.   The antibacterial and bactericidal fiber product according to any one of claims 1 to 7, wherein the fiber product is a mask or a cloth personal product.   The antibacterial and bactericidal fiber product according to any one of claims 1 to 8, wherein the textile product is a medical clothing or a medical cloth personal article.   A method for producing an antibacterial / bactericidal fiber product comprising a fiber containing a photosensitizing dye as a constituent material, comprising the step of dyeing the fiber at a dye bath temperature of 100 to 130 ° C. A method for producing antibacterial and bactericidal fiber products.

Images