JP2005291849A - Whole blood platelet aggregation measuring method, its apparatus, and suction nozzle - Google Patents

Abstract

<P>PROBLEM TO BE SOLVED: To provide a whole blood platelet aggregation measuring method capable of eliminating complicated pretreatments in platelet aggregation tests and easily determining aggregation by automatically performing treatments to test results of platelet aggregation, and provide its apparatus and a suction nozzle to be used for the whole blood platelet aggregation measuring apparatus and of a form effective for automating tests. <P>SOLUTION: Whole blood is inserted in a plurality of cuvettes 19. Platelet-aggregating agents of different concentrations are added to the cuvettes 19 and heated at a prescribed temperature to bring about aggregation reaction for a prescribed time and produce samples. The whole blood with a lower concentration of the platelet-aggregating agent is sequentially drawn in the suction nozzle 19 through its micro-mesh filter by suction. A suction pressure when each sample is passed through the micro-mesh filter is measured to create an aggregation curve. A coefficient of platelet aggregation threshold values in the whole blood is computed on the basis of the aggregation curve and aggregation threshold values. It is judged on the basis of which region includes the coefficient of platelet aggregation threshold values whether platelet aggregation in the whole blood is normal or not in the whole blood platelet aggregation measuring method. <P>COPYRIGHT: (C)2006,JPO&NCIPI

Description

  The present invention relates to a whole blood platelet aggregating ability measuring method for measuring platelet aggregating ability using whole blood without using platelet-rich plasma separated from blood, its device, and aspiration used in a whole blood platelet aggregating ability measuring device. This relates to the nozzle.

  Conventionally, the light transmission method is mainly used for the measurement of platelet aggregation ability. FIG. 8 shows the configuration of the platelet aggregating ability measuring apparatus, and 1 shows a photoelectric aggregation degree measuring means. A cuvette 2 with a stirrer containing platelet-rich plasma (hereinafter referred to as PRP) separated from blood and a platelet aggregation inducer is placed in a heat-retaining tank 3 and stirred. An element 5 is provided so that light from the light source 4 is transmitted through the cuvette 2 and enters the light receiving element 5. In the light receiving element 5, a detection signal based on transmitted light that has passed through PRP and platelet-free plasma (hereinafter referred to as PPP) is input to the time-aggregation rate curve creating means 6 to create an aggregation rate curve with respect to time. . Subsequently, the aggregation rate curve-aggregation ability curve creation means 7 creates an aggregation rate curve from the aggregation rate curve over time, and the aggregation rate curve is compared with the standard aggregation rate curve stored in the memory 9. The means 8 performs collation, and the collation result is output to the printer 10. (For example, see Patent Document 1)

Japanese Patent Publication No. 8-10226 (page 3, right column, lines 38 to 49, FIG. 4)

  In the measurement of platelet aggregation ability by the light transmission method, it is necessary to prepare PRP and PPP from whole blood. First, sodium citrate is added to the collected whole blood to obtain anticoagulated blood, and the blood is centrifuged. The erythrocyte concentrate and PRP are separated, and the erythrocyte concentrate is further centrifuged to separate erythrocytes and PPP. However, PRP contains some white blood cells, red blood cells, etc., and has a problem of affecting the transmitted light, and it is necessary to separate the PRP and PPP from the whole blood sample collected during the platelet aggregation test. There was such a trouble. Moreover, this separation operation has been considered to have some adverse effects on the platelet aggregation ability test. Further, the conventional example has a drawback in that the aggregation rate curve is created after creating the relationship between the aggregation rate of PRP at each concentration of the aggregation-inducing agent and time, and the creation procedure is complicated.

  The present invention has been made in view of the problems as described above, and eliminates the complicated preprocessing in the platelet agglutination ability test, and automatically processes the platelet agglutination ability test result to determine the agglutination ability. The present invention provides a method and apparatus for measuring platelet aggregation ability of whole blood that can be easily performed, and an aspirating nozzle that is used in a whole blood platelet aggregation ability measurement apparatus and is effective in automating examinations. It is what.

  The present invention achieves the above-mentioned problems, and the invention according to claim 1 sets a suction nozzle equipped with a micromesh filter, and adds platelet aggregation-inducing agents having different concentrations to a plurality of cuvettes containing whole blood. After the addition and agglutination reaction at a predetermined temperature for a certain period of time, the suction pressure at the time of passing through the micromesh filter by sucking through the micromesh filter of the suction nozzle sequentially from the whole blood having a low concentration of the platelet aggregation inducer And measuring the platelet aggregation threshold coefficient in the whole blood from the aggregation curve and the aggregation threshold, and depending on which region the platelet aggregation threshold coefficient is included in A method for measuring platelet aggregation of whole blood, comprising determining whether platelet aggregation in whole blood is normal.

  The invention according to claim 2 is also characterized in that a plurality of cuvettes containing whole blood are heated at a predetermined temperature on an adjustment table on which the cuvettes, each of which has been added a platelet aggregation inducer having a different concentration, are added to the platelets. After the agglutination reaction with the inducer for a certain period of time, the adjustment table is transported to the agglutination ability measurement position, and a suction nozzle is attached to the aspiration cylinder at the agglutination ability measurement position. The blood is sequentially moved from the blood to the agglutination capacity measurement position and sucked through the micromesh filter of the suction nozzle, the suction pressure when the whole blood of each cuvette passes through the micromesh filter is measured, and an aggregation curve is created. Calculate the platelet aggregation threshold coefficient in the whole blood from the aggregation curve and the aggregation threshold, determine in which region the platelet aggregation threshold coefficient is included, and whether the platelet aggregation ability in the whole blood is normal or not Whole blood platelet aggregation measuring apparatus characterized by determination and output.

The invention of claim 3 is an adjustment table on which a plurality of cuvettes for containing whole blood and a suction nozzle equipped with a micromesh filter are placed;
A heating means for putting the whole blood into the cuvette and adding the platelet aggregation inducers having different concentrations to each other and placing the adjustment table at a predetermined temperature to cause a sample to undergo an agglutination reaction;
After moving the suction nozzle mounting position of the adjustment table to the agglutination ability measurement position, transport means for sequentially moving from the cuvette containing the low concentration sample of the platelet aggregation inducer to the agglutination ability measurement position;
A suction means for sucking a sample in the cuvette at the coagulation capacity measurement position by a suction cylinder equipped with the suction nozzle;
A suction pressure measuring means for measuring the suction cylinder internal pressure when the sample sucked by the suction means passes through the micromesh filter in the suction nozzle;
After the suction nozzle transported to the coagulation capacity measurement position is inserted into the tip of the suction cylinder, the suction cylinder is moved up and down to insert or withdraw the suction nozzle into the cuvette transported to the cohesion capacity measurement position Means and
The operation timing of the conveying means, the elevating means, and the suction means is adjusted, and each sample is sucked by the suction means by sequentially moving from the cuvette of the low-concentration sample of the platelet aggregation-inducing agent to the position for measuring the aggregation ability. Control means for measuring the suction cylinder internal pressure by the suction pressure measuring means;
Creating means for creating an aggregation curve from the suction pressure measured by the suction pressure measuring means;
Calculating means for calculating a platelet aggregation threshold coefficient from the aggregation threshold of the aggregation curve;
Whole blood platelet aggregation capacity measurement, comprising: agglutination capacity determination means for determining in which range the platelet aggregation threshold coefficient is included and determining whether or not the platelet aggregation capacity is normal Device. The control means has a function of controlling the heating means to a constant temperature.

  According to a fourth aspect of the present invention, the suction nozzle body comprises a substantially tapered suction tube having one suction end of a suction tube having a small diameter as a suction port and the other opening end having a large diameter as an attachment / detachment portion. A stepped portion having a widened diameter of the suction tube extending from the mouth is provided between the suction port and the attaching / detaching portion, and a micromesh filter that prevents passage of aggregates due to platelets is attached to the stepped portion, and the suction A suction nozzle for use in a whole blood platelet aggregating capacity measuring device, wherein a plurality of ribs are provided in the periphery of the suction tube from the mouth to the stepped portion in the direction of the suction tube axis.

  In the invention of claim 1, after setting a suction nozzle equipped with a micromesh filter, after adding platelet aggregation inducers having different concentrations to a plurality of cuvettes containing whole blood and agglutinating reaction for a certain time at a predetermined temperature, Sequentially from the whole blood with a low concentration of the platelet aggregation-inducing agent, the suction pressure when passing through the micromesh filter by sucking through the micromesh filter of the suction nozzle, and creating an aggregation curve of the whole blood, The platelet aggregation threshold coefficient of the whole blood is calculated from the aggregation curve and the aggregation threshold, and whether or not the platelet aggregation ability in the whole blood is normal depending on which region the platelet aggregation threshold coefficient is included in This is a method for measuring whole blood platelet agglutination ability, and is a method for measuring platelet agglutination ability using whole blood, and it is not necessary to perform complicated pretreatment, and an aggregation curve can be immediately created from the suction pressure. In addition, the platelet aggregation threshold coefficient can be easily calculated from the platelet aggregation threshold of the aggregation curve, and the platelet aggregation ability of blood can be easily determined based on this platelet aggregation threshold coefficient. There is an advantage that a measurement method can be provided.

  In the invention of claim 2, the adjustment table on which the cuvettes, each of which has been added with platelet aggregation-inducing agents having different concentrations, is heated at a predetermined temperature to a plurality of cuvettes containing whole blood, and the whole blood is treated with the platelet aggregation-inducing agent. After the agglutination reaction for a certain period of time, the adjustment table is transported to the agglutination capacity measurement position, and a suction nozzle is attached to the aspiration cylinder at the agglutination capacity measurement position. Sequentially moved to the aggregating capacity measurement position, sucked through the micromesh filter of the suction nozzle, measured the suction pressure when the whole blood of each cuvette passes through the micromesh filter, and created an agglutination curve. The platelet aggregation threshold coefficient in the whole blood is calculated from the aggregation threshold and the platelet aggregation threshold coefficient in the whole blood is determined by determining in which region the platelet aggregation threshold coefficient is included. The A whole blood platelet aggregating capacity measuring device characterized in that the whole blood is put into a plurality of cuvettes, and platelet aggregation inducing agents having different concentrations are added to the respective cuvettes and set on an adjustment table at a predetermined temperature. After the agglutination reaction, the aggregation ability is measured using the same suction nozzle, and the whole blood with a low concentration of the platelet aggregation inducer of each sample is passed through the micromesh filter, and suction is performed at that time. By measuring the pressure, an aggregation curve can be easily created, and the platelet aggregation threshold coefficient is calculated from the platelet aggregation threshold of the aggregation curve, and the platelet aggregation threshold coefficient is included in any range to facilitate blood platelet aggregation ability There is an advantage that can be judged.

  In the invention of claim 3, an adjustment table on which a plurality of cuvettes for containing whole blood and a suction nozzle equipped with a micromesh filter are placed, and a platelet agglutination-inducing agent having different concentrations are introduced into the cuvette. A heating means for heating the adjustment table added and placed at a predetermined temperature to obtain a sample subjected to agglutination reaction, and after moving the suction nozzle placement position of the adjustment table to the aggregation capacity measurement position, platelets Conveying means for sequentially moving from a cuvette containing a low concentration sample of the agglutination-inducing agent to the agglomeration capacity measurement position, and a suction means for aspirating the sample in the cuvette at the aggregation capacity measurement position by a suction cylinder equipped with the suction nozzle And suction pressure measuring means for measuring the suction cylinder internal pressure when the sample sucked by the suction means passes through the micromesh filter in the suction nozzle; After the suction nozzle transported to the coagulation capacity measurement position is inserted into the tip of the suction cylinder, the suction cylinder is moved up and down to insert or withdraw the suction nozzle into the cuvette transported to the cohesion capacity measurement position Means for adjusting the operation timing of the conveying means, the elevating means, and the suction means, and sequentially moving from the cuvette of the low-concentration sample of the platelet agglutination-inducing agent to the agglutination ability measurement position by the suction means. Control means for sucking each sample and measuring the suction cylinder internal pressure by the suction pressure measuring means, creation means for creating an aggregation curve from the suction pressure measured by the suction pressure measuring means, and an aggregation threshold for the aggregation curve Calculating means for calculating the platelet aggregation threshold coefficient from the above, and determining in which range the platelet aggregation threshold coefficient is included, and the platelet aggregation ability is normal Since the whole blood platelet aggregating capacity measuring device is provided with an aggregating capacity determining means for determining whether or not the adjustment table is agglomerated by the control means with the operation timing of the conveying means, the elevating means and the aspirating means. The suction nozzle is attached to the suction cylinder, the sample is moved from the low concentration sample of the platelet aggregation inducer to the aggregation ability measurement position, and each sample is sucked by the suction means and sucked by the suction pressure measurement means. A whole blood platelet aggregating capacity measuring device that can measure the internal pressure of the cylinder, can measure the platelet aggregating capacity at a constant aggregation reaction time and temperature until measurement, and can measure the aggregating capacity with high reliability. There are benefits that can be provided.

  In the invention of claim 4, the suction nozzle body comprises a substantially tapered suction tube having one opening end of a suction tube having a small diameter as a suction port and the other opening end having a large diameter as an attachment / detachment portion. A stepped portion having a widened diameter of the extending suction tube is provided between the detachable portion from the suction port, and a micromesh filter that prevents passage of aggregates due to platelets is attached to the stepped portion, and from the suction port. A suction nozzle used in a whole blood platelet aggregating capacity measuring device, wherein a plurality of ribs are provided around the suction pipe outer periphery to the stepped portion in the direction of the aspirating axis, and the whole blood platelet aggregating capacity measuring device When the suction nozzle is lowered in the vertical direction and inserted into the cuvette, if there is even a slight stepped portion in the suction nozzle, the stepped portion will come into contact with the cuvette and jump, making automation difficult. But the stage Part is to enable automation of a plurality of ribs so as not to contact the cuvette are circumferentially provided, aggregation measured suction nozzle so as to ensure correct insertion into a cuvette.

  DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Embodiments of a whole blood platelet aggregating ability measuring method, an apparatus thereof, and a suction nozzle according to the present invention will be described below with reference to the drawings. FIG. 1 is a perspective view of a whole blood platelet aggregating ability measuring device 11 of the present embodiment. In the figure, the apparatus main body 12 in which the coagulation capacity measuring mechanism and the transport mechanism are incorporated has a pre-reaction stage section provided with a heating means for placing and heating a sample on an adjustment table (hereinafter referred to as a measurement rack). 13, a display unit 14 such as a liquid crystal display on which operation contents and measurement results are displayed, and an operation unit 15 including a numeric keypad and operation buttons for operating the apparatus are provided. It has been. The printer 16 may be incorporated in the apparatus main body 12.

  In the preliminary reaction stage unit 13, the measurement rack 18 on which the four cuvettes 19 and the suction nozzle 20 are placed is heated to a constant temperature by the heater 17, and the whole blood in the cuvette 19 and the platelet aggregation inducer are Is stirred by a stirring means 25 such as a stirrer and the agglomeration reaction is performed for a certain time. The measurement rack 18 on which the cuvette 19 is placed is sent from the opening 12a to the coagulation capacity measurement unit in the apparatus main body 12. As shown in FIG. 5, the preliminary reaction stage unit 13 is provided with a temperature sensor 17a adjacent to the heater 17, and the measurement rack 18, the cuvette 19, the suction nozzle 20, and the sample in the cuvette 19 have a constant temperature. So that it is controlled. The positions of the measurement racks 18a and 18b are preliminary heating units, and the position of the measurement rack 18c is a measurement start position. Directly below the measurement rack 18, a stirrer (stirring device) that rotates the stirring rod (metal piece) in the cuvette 19 to stir the sample is provided for each cuvette 19.

  In the apparatus main body 12, as shown in FIG. 2, a transport mechanism 30 for transporting the measurement rack 18 from the measurement start position to the agglutination ability measurement position, and an elevating means and a suction means for measuring the agglutination ability of whole blood. And the power supply and control device 24 are incorporated. The control device 24 controls the temperature of the preliminary reaction stage 13 and measures the coagulation ability in the coagulation ability measurement. Timing control is performed such as conveyance to a position, elevating means and aspiration means for aspirating the sample.

  In the transport mechanism 30, a transport base 31 on which the measurement rack 18 is placed is coupled to a slider 33 via a connecting member 32. The slider 33 is screwed into a screw rod 35 pivotally supported by the bearings 34a and 34b, and the other end of the screw rod 35 is rotatably connected to the rotation shaft of the motor 36 by a coupling member. By driving the motor 36, the measurement rack 18 on which the sample is placed can be transported from the measurement start position to the coagulation ability measurement position, as indicated by the arrow X, and after the coagulation ability measurement is completed. The measurement rack 18 can be transported from the aggregation capacity measurement position to the measurement start position.

  As shown in FIGS. 3A and 3B, the aggregating capacity measuring mechanism 40 is provided with a suction cylinder 41 to which the suction nozzle 20 is attached, and in the suction cylinder 41, an air passage 42 a is provided at the shaft core. The suction piston rod 42 is internally provided. The suction piston rod 42 is provided with an air passage 42 a communicating with the inside of the suction cylinder 41, and the air passage 42 a is provided with a pressure sensor 43 for measuring the air pressure in the air passage 42 a and the suction cylinder 41. And a control valve 44 that shuts off or opens the internal pressure and the external pressure of the air passage 42a. The suction piston rod 42 is fixed to a slider 48 which is a drive mechanism for moving the suction piston rod 42 up and down, and a screw rod 47 screwed to the slider 48 is pivotally supported by bearings 49a and 49b and provided at one end of the screw rod 47. The pulley is interlocked by a drive belt 46 that is stretched over a pulley provided on the rotation shaft of the motor 45, and the slider 48 moves up and down in response to the drive of the motor 45. The suction piston rod 42 connected to the slider 48 moves up and down in the suction cylinder 41 as the slider 48 moves up and down. The aggregating capacity measuring mechanism 40 includes a piston mechanism using a screw rod 47, a slider 48, and the like, and a suction cylinder 41 in which a suction piston rod 42 is housed, and the aggregating capacity measuring mechanism 40 is driven by a lifting mechanism 50 driven by a motor. As shown by the arrow Y, it is configured to move up and down in the vertical direction.

  In the present embodiment, when the measurement rack 18 on which the cuvette 19 and the suction nozzle 20 are placed is transported to the coagulation capacity measurement position, the suction cylinder 41 is lowered by the lifting mechanism 50 to measure the measurement rack 18 at the coagulation capacity measurement position. The suction nozzle 20 is attached to the lower end tip 41a of the suction cylinder 41, and the suction cylinder 41 is raised. Subsequently, the cuvette 19 placed on the measurement rack 18 is moved to the coagulation capacity measurement position by the conveyance means by the conveyance mechanism 30, and the suction cylinder 41 equipped with the suction nozzle 20 is lowered by the lifting mechanism 50. The suction nozzle 20 is inserted into the cuvette 19 and inserted into the sample to stop the descent. In a state where the control valve 44 is closed, the suction piston rod 42 is lifted to make the suction cylinder 41 have a negative pressure and suck the sample through the micromesh filter. The pressure in the suction cylinder 41 is measured by the pressure sensor 43. After the measurement, the control valve 44 is opened, the pressure in the suction cylinder 41 becomes the same as the external atmospheric pressure, and the sucked sample is returned into the cuvette 19 through the micromesh filter. The same operation is repeated again, and the suction pressures of the samples in all the qubits 19 placed on the measurement rack 18 are measured. When the measurement is completed, the aggregating capacity measuring mechanism 40 is raised to a height higher than a predetermined level, the suction nozzle 20 is brought into contact with the contact portion 51, and the suction nozzle 20 is separated from the tip portion 41a of the suction cylinder 41. Return to position. These operations are performed at the coagulation ability measurement position. The measurement rack 18 on which the cuvette 19 and the suction nozzle 20 for which the measurement has been completed is returned to the preliminary reaction stage unit 13 through the opening 12a from the coagulation capacity measurement position.

  Next, the suction nozzle 20 will be described in detail with reference to FIG. The suction nozzle body 21 is a tapered tube including suction tubes 21c and 21d. The diameter of the flow path of the front end suction pipe 21c is smaller than the diameter of the flow path of the rear end suction pipe 21d, and the front end suction pipe 21c. And the rear end suction tube 21d is widened to form a stepped portion 21f. A micromesh filter 22 is mounted on the rear end suction pipe 21d side of the stepped portion 21f and fixed with a packing 23. The distal end suction tube 21c is a suction port 21a through which blood is sucked, and the open end of the rear end suction tube 21d is a detachable portion 21b to which the lower end distal end portion 41a of the suction cylinder 41 is attached. As shown in FIGS. 4A and 4B, ribs 21e formed in the axial direction are provided around the outer peripheral portion of the distal end tube 21c at equal intervals.

  The suction nozzle 20 is formed of a resin such as polypropylene, and the cut surface of the suction port 21a has an inclined surface so that the sample can be sucked easily. The impact can be absorbed even when the tip of the slab contacts the bottom of the cuvette 19. The micromesh filter 22 is a nickel filter having a thickness of 20 μm, and the filter has a square shape of 30 × 30 μm.

  Next, with reference to FIGS. 1-7, the platelet aggregation ability measurement by the whole blood of this embodiment is demonstrated. First, whole blood is placed in four cuvettes 19 and placed on the preliminary reaction stage 13 at the preliminary heating position A (see FIG. 5), and a platelet aggregation inducer such as ADP (Adenosine Di-Phsphat) 4 Adjust the concentration to the step concentration (0.5 μM (mol), 1.0 μM, 2.0 μM, 4.0 μM) and draw each concentration of ADP with a 4-channel micropipette. The thin ADP side is aligned with the cuvette 19 on the suction nozzle 20 side of the measurement rack 18, the ADP having the highest concentration is aligned with the cuvette 19 on the near side, and each concentration of ADP is put into the four cuvettes 19. Create a sample. Similar samples are prepared in the measurement rack 18 at the preliminary heating position C of the preliminary reaction stage unit 13, and the samples in the measurement racks 18a and 18b are stirred at the preliminary heating positions A and C with a stirrer at a constant temperature. The mixture is heated to (37 ° C.), preheated, and subjected to an agglomeration reaction for a predetermined time. The measurement rack 18a at the preheating position A is moved to the central measurement start position C, and an agglutination reaction is performed for 5 minutes.

  By operating the measurement start button of the operation unit 15, the measurement rack 18 a at the measurement start position C operates the transport unit 30, and the measurement rack 18 C is sent from the opening 12 a to the measurement position of the coagulation capacity measurement unit in the apparatus main body 12. It is. First, in the measurement rack 18C, the placement position of the suction nozzle 20 stops at the cohesive capacity measurement position.

  Subsequently, the suction cylinder 41 is lowered by the elevating mechanism 50, and the suction nozzle 20 is attached to the distal end portion 41a of the suction cylinder 41 to be raised and stopped. The cuvette 19 of the sample loaded with ADP 0.5 μM is moved by the operation of the transport means 30, and the suction cylinder 41 fitted with the suction nozzle 20 is lowered again by the lifting mechanism 50 into the cuvette 19. Plugged in and stopped. The control valve 44 is closed, the suction piston rod 42 of the suction means 40 is raised, and the sample in the cuvette 19 is sucked. The sample is sucked from the suction port 21a, passes through the micromesh filter 22 in the suction nozzle 20 via the tip suction pipe 21C, and is sent to the rear end suction pipe 21d of the suction nozzle 20. The suction pressure in the ventilation passage 42 a of the suction piston rod 42 at that time is measured by the pressure sensor 43, and the measured value is input to the control device 24. When the measurement of the suction pressure in the suction cylinder 41 is completed, the suction nozzle 20 is lowered, the control valve 44 is opened, the outside air flows into the suction cylinder 41 from the air passage 42a, and the air pressure in the suction cylinder 41 is the outside air pressure. And the sample in the suction nozzle 20 is returned to the cuvette 19.

  When the suction pressure of the sample in the first cuvette 19 is measured, the suction nozzle 20 rises and stops, the transport means 30 is driven, the measurement rack 18 moves rearward, and ADP 1.0 μM is inserted. The cuvette 19 is moved to the measurement position, a similar suction operation is performed, the sample in the cuvette 19 is sucked, and the suction pressure in the suction cylinder 41 is measured by the pressure sensor 43. With this procedure, the suction pressure of the sample to which ADP of 0.5 μM (mol), 1.0 μM, 2.0 μM, and 4.0 μM has been added is measured, and the suction pressure value is stored in the storage device of the control device 24. Remembered. When the measurement of all the samples in the measurement rack 18 is completed, the measurement rack 18 moves to the collection position (aggregation capacity measurement position) of the suction nozzle 20 and stops, the suction nozzle 41 is raised by a predetermined height or more, and the suction cylinder The suction nozzle 20 attached to the tip 41a of 41 is brought into contact with the contact portion 51, removed and dropped into the measurement rack 18, and returned to a predetermined position of the measurement rack 18. The measurement rack 18 moves forward, is returned to the measurement start position, and is collected. The sample of the next measurement rack 18b is placed at the measurement start position, and the aggregation measurement is started again in the same point order. In this way, the measurement of all the samples of the cuvette 19 placed on the measurement rack 18 can be completed in a short time, and all the samples are aggregated by the same suction nozzle 20 in a substantially constant agglutination reaction time. Performance measurement. The measurement result is stored in the storage unit of the control device 24 and output to the display unit 14 or the printer 16. The printer 16 outputs the inspection form shown in FIG.

  In this embodiment, in order to find the lowest concentration of ADP that is estimated that platelets caused by platelets cause secondary aggregation, whole blood to which ADP having a different concentration is added is prepared, and the whole blood is subjected to agglutination reaction. The agglutination curve is prepared by measuring the suction pressure according to the clogging state of the micromesh filter 22 due to the agglomeration caused by the next aggregation, and based on this aggregation curve, the platelet aggregation threshold (PATI: Platelet aggregatory threshold index) The aggregation threshold coefficient is obtained, and the platelet aggregation ability of the blood is examined according to which range the platelet aggregation threshold coefficient belongs.

  Next, the platelet agglutination ability test form will be described in detail with reference to FIG. This inspection form is provided with printing areas R1 to R5, and R1 is printed with measurement date, measurement time, measurement mode (ANALY 1), agglutination reaction time (minutes and seconds), and measurement number (specimen number). Then, the drug name, unit (ADP, μM), and reagent concentration (0.5, 1.0, 2.0, 4.0 (μM)) of the agglutination reagent (platelet aggregation inducer) are printed. A measurement result is shown in the print region R2, a platelet aggregation threshold coefficient (PATI coefficient) is shown, and a grade of aggregation ability is shown. Grade 1 (normal) is printed on this inspection form. PATI indicates the minimum concentration of a platelet aggregation inducer that induces secondary aggregation, and is the value of the reagent concentration when the suction pressure is 50%, and this value is used as the platelet aggregation threshold coefficient.

  A graph is shown in the printing region R3, the horizontal axis indicates 0 to 4 channels (reagent concentration), the vertical axis indicates the suction pressure (0 to 100%), and the range of the coagulation ability grade is indicated by diagonal lines. The agglomeration curve α is illustrated, and the measurement results are indicated by triangles in the grade type (GT) column. In the print region R4, III, II, I, -I, and -II are displayed as GT, and I is indicated by a triangle mark. In the printing region R5, the suction pressure when whole blood is not sucked is shown in 0ch, and the concentration of ADP (0.5 μM, 1.0 μM, 2.0 μM, 4.0 μM) is shown in 1-4 channels (ch). In the measurement result of this inspection form, -3.1%, 0.7%, 3.0%, 73.5%, and 100.0% are displayed. In the percentage of the printing area R5, 4ch is 100.0%, and the result of this suction pressure indicates that the micromesh filter 22 in the suction nozzle 20 is completely clogged, and the percentage value decreases. The degree of clogging is reduced. That is, the aggregation curve α is a graph of the clogged state. The grade of aggregating ability is evaluated based on the range (area divided by diagonal lines in the graph) where the value (PATI value coefficient) at which the aggregation curve α crosses the PATI value (clogging is 50%). Yes.

  The aggregation curve α is a straight line between the channels, and these straight lines are represented by linear functions. Therefore, a PATI value, that is, a value obtained by substituting a known 50% suction pressure value into the linear function of the straight line. Is a PATI value coefficient, and the PATI value coefficient can be calculated by a simple arithmetic expression. Which range the PATI value coefficient belongs to can be easily obtained from a comparison value indicating a grade range. Incidentally, the comparison values are, for example, grade III is −1 to 0 μM, grade II is 0 to 1 μM, grade I is 1 to 2 μM, grade 0 is 2 to 4 μM, and the PATI coefficient is 1.67 μM. I. The grade can be judged by a coagulation ability judging means by simple comparison. These calculation formulas and coagulation ability determination means can be executed by calculation means by the control device 24.

  Further, as shown in FIG. 7, the suction nozzle 20 of the present embodiment prevents the stepped portion of the suction nozzle 20 from coming into contact with the upper end portion of the cuvette 19 when the suction nozzle 20 is inserted into the cuvette 19. Therefore, even if the rib 21e is provided and the placement position of the cuvette 19 is biased, the suction nozzle 20 is inserted into the cuvette 19 without contacting the upper end of the cuvette 19 by the rib 21e. Can do. As a result, it was possible to automatically measure the aggregation ability in a short time. Further, a magnet 52 is arranged at the coagulation capacity measurement position so that the stirrer stirring rod 25a is attracted to one side, and the stirring rod 25a blocks the suction port 21a of the suction nozzle 20 to increase the suction pressure. It is preventing.

  In this embodiment, the agglutination ability measurement is performed based on four samples of whole blood. However, the present invention is not limited to this embodiment, and eight samples can be measured.

  The present invention is a whole blood platelet aggregating capacity measuring device capable of measuring the blood aggregating ability by the whole blood method, and the aggregating ability is automatically measured from the agglutination reaction to the aggregating capacity measurement, Compared with the simpler method, the result can be obtained immediately, and the agglutination test result can be immediately used for diagnosis in the medical field.

It is a perspective view which shows one Embodiment of the whole blood platelet aggregation ability measuring apparatus of this invention. It is the schematic which shows the control system of the whole blood platelet aggregation ability measuring apparatus of this embodiment, and shows an internal structure. (A) is a schematic side view including principal part sectional drawing of the attraction | suction mechanism of this embodiment, (b) is a schematic front view including principal part sectional drawing. (A) is sectional drawing of a suction nozzle, (b) is a front part, (c) is II cross-sectional part. It is a schematic top view which shows each part arrangement | positioning of this embodiment. It is a figure which shows the platelet aggregability test | inspection form of this embodiment. It is explanatory drawing at the time of the platelet aggregation ability test | inspection of the suction nozzle in this embodiment. It is the schematic of the conventional platelet aggregation ability measuring apparatus.

Explanation of symbols

DESCRIPTION OF SYMBOLS 11 Whole blood platelet aggregation measuring apparatus 12 Apparatus main body 12a Opening part 13 Preliminary reaction stage part 14 Display part 15 Operation part 16 Printer 17 Heater 17a Temperature sensor 18, 18a, 18b, 18c Measurement rack 19 Cuvette 20 Suction nozzle 21 Suction nozzle main body DESCRIPTION OF SYMBOLS 22 Micromesh filter 23 Packing 24 Control apparatus 25 Stirrer etc. Stirring means 30 Conveyance mechanism 31 Conveyance stand 32 Connecting member 33 Slider 34a, 34b Bearing 35 Screw rod 36 Motor 40 Aggregation capacity measurement mechanism 41 Suction cylinder 42 Suction piston rod 43 Pressure sensor 44 Control valve 50 Elevating mechanism

Claims (4)

  After setting a suction nozzle equipped with a micromesh filter and adding platelet aggregation inducers with different concentrations to a plurality of cuvettes containing whole blood and agglutinating reaction for a certain time at a predetermined temperature, the platelet aggregation inducer is low Sequentially from the whole blood, the suction pressure when sucking through the micromesh filter of the suction nozzle and passing through the micromesh filter is measured, an aggregation curve of the whole blood is created, the aggregation curve and the aggregation threshold The platelet aggregation threshold coefficient of the whole blood is calculated from, and it is determined whether or not the platelet aggregation ability in the whole blood is normal depending on which region the platelet aggregation threshold coefficient is included in Whole blood platelet aggregation measuring method.   After heating the adjusting table on which the cuvettes with different concentrations of platelet aggregation-inducing agents added to a plurality of cuvettes containing whole blood are heated at a predetermined temperature, the adjusting table is allowed to agglutinate for a certain period of time. After transporting to the measurement position and attaching the suction nozzle to the suction cylinder at the aggregation capacity measurement position, the blood is sequentially moved from the low concentration whole blood of the platelet aggregation inducer to the aggregation capacity measurement position, and the micromesh of the suction nozzle Aspirate through the filter, measure the aspiration pressure as whole blood in each cuvette passes through the micromesh filter, create an aggregation curve, and determine the platelet aggregation threshold coefficient in the whole blood from the aggregation curve and aggregation threshold Calculating and outputting a measurement result by determining whether or not the platelet aggregation ability in the whole blood is normal depending on which region the platelet aggregation threshold coefficient is included in Platelet aggregation ability measurement apparatus. An adjustment table for mounting a plurality of cuvettes for containing whole blood and a suction nozzle equipped with a micromesh filter;
A heating means for putting the whole blood into the cuvette and adding the platelet aggregation inducers having different concentrations to each other and placing the adjustment table at a predetermined temperature to cause a sample to undergo an agglutination reaction;
After moving the suction nozzle mounting position of the adjustment table to the agglutination ability measurement position, transport means for sequentially moving from the cuvette containing the low concentration sample of the platelet aggregation inducer to the agglutination ability measurement position;
A suction means for sucking a sample in the cuvette at the coagulation capacity measurement position by a suction cylinder equipped with the suction nozzle;
A suction pressure measuring means for measuring the suction cylinder internal pressure when the sample sucked by the suction means passes through the micromesh filter in the suction nozzle;
After the suction nozzle transported to the coagulation capacity measurement position is inserted into the tip of the suction cylinder, the suction cylinder is moved up and down to insert or withdraw the suction nozzle into the cuvette transported to the cohesion capacity measurement position Means and
The operation timing of the conveying means, the elevating means, and the suction means is adjusted, and each sample is sucked by the suction means by sequentially moving from the cuvette of the low-concentration sample of the platelet aggregation-inducing agent to the position for measuring the aggregation ability. Control means for measuring the suction cylinder internal pressure by the suction pressure measuring means;
Creating means for creating an aggregation curve from the suction pressure measured by the suction pressure measuring means;
Calculating means for calculating a platelet aggregation threshold coefficient from the aggregation threshold of the aggregation curve;
Aggregating ability determination means for determining in which range the platelet aggregation threshold coefficient is included and determining whether or not the platelet aggregating ability is normal;
An apparatus for measuring platelet aggregation ability of whole blood, comprising:   The suction nozzle body is composed of a substantially tapered suction tube having one opening end of a suction tube having a small diameter as a suction port and the other opening end having a large diameter as an attachment / detachment portion, and the suction tube extends from the suction port. A stepped portion having an enlarged diameter is provided between the suction port and the attaching / detaching portion, a micromesh filter that prevents passage of aggregates due to platelets is attached to the stepped portion, and a suction tube from the suction port to the stepped portion A suction nozzle for use in a whole blood platelet aggregating capacity measuring device, wherein a plurality of ribs are provided around the outer periphery in the direction of the suction tube axis.

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