JP2007143473A - Food and drink using buckwheat as raw material and method for producing the same - Google Patents
Food and drink using buckwheat as raw material and method for producing the same Download PDFInfo
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- Coloring Foods And Improving Nutritive Qualities (AREA)
- Cereal-Derived Products (AREA)
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Abstract
Description
本発明は、ソバを原料としたγ−アミノ酪酸(以下、「GABA」と略称する)の含量が増強された飲食品及びその製造方法に関し、より詳しくは、ソバの種子の殻を取り除いて得たソバの実、もしくはソバの種子を蒸熱してアルファー化し、殻を取り除いて得たソバの実を粉砕し、粉砕物の中で乾燥基準でタンパク質含量が20重量%以上の高タンパク質区分を分取し、これに麹菌を接種、培養して麹化させ、焙煎又は蒸熱する、ソバを原料とした飲食品及びその製造方法に関する。 The present invention relates to a food and drink having an increased content of γ-aminobutyric acid (hereinafter abbreviated as “GABA”) using buckwheat as a raw material and a method for producing the same, and more specifically, by removing the buckwheat seed shell. The buckwheat seeds or buckwheat seeds are steamed and alphalated, and the buckwheat seeds obtained by removing the shells are pulverized to classify the high protein fraction with a protein content of 20% by weight or more on a dry basis in the pulverized product. The present invention relates to foods and drinks made from buckwheat, which are inoculated, inoculated with koji mold, cultured, hatched, roasted or steamed, and a method for producing the same.
近年は、健康志向の高まりに伴い、年齢にこだわらず広い年代層の人々に、カフェインなどの刺激物質を含有しない低糖分の飲料、例えば穀物を原料とした茶飲料等が好まれ、この種の飲料がペットボトルに収納され広く市販されている。穀物飲料の原料の一つであるソバは、タンパク質、ビタミン、ミネラル等の栄養素を豊富に含み、特にダッタンソバは血圧降下に有効とされるルチンも多量に含んでいることから、従来、ソバを原料とした飲食品やその製造方法は数多く提案されている。 In recent years, with an increase in health consciousness, people of all ages, regardless of age, prefer low-sugar beverages that do not contain stimulating substances such as caffeine, such as tea beverages made from cereals. Beverages are stored in plastic bottles and are widely available on the market. Buckwheat, one of the ingredients of cereal drinks, is rich in nutrients such as protein, vitamins, and minerals. In particular, tartary buckwheat contains a large amount of rutin, which is effective in lowering blood pressure. Many foods and beverages and their manufacturing methods have been proposed.
例えば、本出願人による、ソバの種子を蒸煮してアルファー化した後、殻を取り除き、10〜60メッシュに粉砕し、さらに焙煎前に対して焙煎後の容積比が1.0〜2.0になるよう焙煎して、ソバを原料とする飲食品の製造法(例えば、特許文献1参照)や、苦ソバ(Fagopyrum tararicum ダッタンソバ)の穀粒から苦ソバ茶を製造する際に、該苦ソバの穀粒を水に浸漬して穀粒の中心部まで水を浸透させた後、蒸して前記穀粒をアルファ化し、次いで、蒸した穀粒を乾燥してから実と殻とを分離した後、前記実を焙煎する苦ソバ茶の製造方法(例えば、特許文献2参照)等が知られている。これらの飲食品は、普通の煎茶と同じように熱湯で煮出して飲むことができるが、原料によってその質が異なり、普通ソバ、ダッタンソバ等それぞれ特有の味及び香り、まろやかさを有する飲料として既に市販されている。 For example, after the buckwheat seeds are steamed and alphalated by the present applicant, the shell is removed, pulverized to 10 to 60 mesh, and the volume ratio after roasting is 1.0 to 2 before roasting. When roasting to 0.0 and producing bitter buckwheat tea from the grain of buckwheat (Fagopyrum tararicum tartary buckwheat) After immersing the bitter buckwheat in water and allowing water to penetrate into the center of the kernel, steam to alphalize the kernel, and then dry the steamed kernel before drying the berries and shells. A method for producing bitter buckwheat tea (for example, see Patent Document 2) in which the fruit is roasted after separation is known. These foods and drinks can be boiled in hot water in the same way as ordinary sencha, but their quality differs depending on the raw materials, and they are already on the market as drinks with a unique taste, aroma and mellowness such as ordinary buckwheat and tartary buckwheat. Has been.
また、ソバ原料をある種の微生物で発酵させる技術として、アレルゲンタンパク質を含む蕎麦粉へ水を加える工程と、水を加えた蕎麦粉を加熱処理して冷却する工程と、この冷却した蕎麦粉に麹菌を接種培養する工程と、その後水を加えて温度40℃〜60℃で加熱する工程と、さらにろ過および濃縮して蕎麦発酵エキスを抽出する工程とを有するアレルゲンタンパク質を除去した蕎麦発酵エキスの製造方法(例えば、特許文献3参照)や、蒸煮したソバ、例えばソバ焼酎粕に麹菌を作用させ発酵させた後、アルコールを主とする揮発成分を取り除いてなり、アミノ酸類を豊富に含有した食品素材(例えば、特許文献4参照)等が知られている。 In addition, as a technology for fermenting buckwheat raw materials with certain microorganisms, a step of adding water to oat flour containing allergen protein, a step of heat-treating and cooling the oat flour containing water, and the cooled oat flour An oat fermented extract from which allergen proteins have been removed, comprising a step of inoculating and culturing koji mold, a step of adding water and heating at a temperature of 40 ° C. to 60 ° C., and a step of further filtering and concentrating to extract the fermented oat extract. A food containing abundant amino acids after removing a volatile component mainly composed of alcohol after fermenting a koji mold with boiled buckwheat, for example, buckwheat shochu, for example, by a production method (see, for example, Patent Document 3) A material (for example, see Patent Document 4) is known.
さらに、蒸したソバの実に、麹、ラギなどのスターターの形態の糸状菌、または糸状菌を直接添加し、ソバの実中のタンパク質を発酵・分解させて大豆加工品であるテンペ様に凝集させ、固形、半固形、または半流動状のソバ食品を得る発酵ソバ食品(例えば、特許文献5参照)や、そばを原料とし、麹菌、酵母、乳酸菌、ビフィズス菌、枯草菌、酢酸菌の中から選ばれる少なくとも1種類の菌を用い、発酵工程を経て製造される血糖値低下作用を持つ健康食品(例えば、特許文献6参照)が知られている。 In addition, starter-shaped filamentous fungi such as persimmon and ragi, or filamentous fungi are added directly to steamed buckwheat, and the protein in the buckwheat is fermented and decomposed to agglomerate into a tempe that is a processed soybean product. , Fermented buckwheat food to obtain solid, semi-solid, or semi-fluid buckwheat food (see, for example, Patent Document 5), buckwheat, yeast, lactic acid bacteria, bifidobacteria, Bacillus subtilis, acetic acid bacteria A health food having a blood glucose lowering effect produced through a fermentation process using at least one selected bacterium is known (see, for example, Patent Document 6).
一方、神経伝達物質であるGABAは、動物ばかりでなく自然界に広く存在しており、血圧降下作用、精神安定作用、肥満防止作用等の健康維持意識の高い現代人にとって有効な生理作用を有している。その上、GABAは人が多量に摂取しても副作用が無いので、安全性の面でも有利であり、食事療法が効果的な生活習慣病、特に血圧症を予防する成分として食品に付加させる開発が多くなされている。 On the other hand, GABA, which is a neurotransmitter, is widely present not only in animals but also in the natural world and has an effective physiological action for modern people with high health maintenance awareness such as blood pressure lowering action, tranquilization action, obesity prevention action, etc. ing. In addition, GABA is advantageous in terms of safety because it has no side effects even if it is consumed in large quantities by humans, and it is a development that is added to food as an ingredient to prevent lifestyle-related diseases, especially blood pressure disorders, where diet therapy is effective. There have been many.
米糠等から高含有GABAの飲食品の製造として、米糠等に、グルタミン酸デカルボキシラーゼ生産能を有する乳酸菌を作用させることによりGABAを高含有した食品素材の製造方法(たとえば、特許文献7参照)や、ポリフェノールを含む穀類の外皮部分の水抽出液を有用微生物の存在下に低温で保持することを特徴とするGABAを含む飲食品組成物の製造法(例えば、特許文献8参照)が知られている。 As a method for producing a high-content GABA food or drink from rice bran or the like, a method for producing a food material highly containing GABA by causing a lactic acid bacterium having glutamic acid decarboxylase producing ability to act on rice bran or the like (see, for example, Patent Document 7), A method for producing a food / beverage composition containing GABA, characterized in that an aqueous extract of the husk portion of cereals containing polyphenols is maintained at a low temperature in the presence of useful microorganisms (for example, see Patent Document 8) is known. .
ソバの実を原料に用いた飲食品は、タンパク質、ビタミン、ミネラル等の栄養素を豊富に含み、特にダッタンソバでは血圧降下に有効とされるルチンも多量に含んでいるが、本発明は、従来のものよりもさらに生理活性作用を有する付加価値の高いソバを原料とした飲食品及びその製造方法を提供することにある。 Foods and drinks using buckwheat nuts as raw materials contain abundant nutrients such as proteins, vitamins, and minerals, and in particular, tartary buckwheat contains a large amount of rutin, which is effective in lowering blood pressure. An object of the present invention is to provide a food and drink using a high-value-added buckwheat having a physiologically active action as compared with that of food and a method for producing the same.
ソバの実を原料に用いた飲食品は、タンパク質、ビタミン、ミネラル等の栄養素を豊富に含み、タンパク質については原料の乾燥ソバ100g当たり10〜15重量%含んでいる。また、ダッタンソバでは血圧降下に有効とされるルチンを多量に含んでおり、ソバ原料を通常の処理をして焙煎して得られる従来のソバ茶等は、これらの作用や、その香り、風味の点等では満足できるものであるが、最近では、ソバの実を原料として微生物で発酵させ、ソバアレルゲンタンパク質を除去したり、アミノ酸等を増加させる技術等があるが、有効に利用できる生理活性物質の生成量は未だ十分とはいえなかった。本発明者は、研究を進め、ソバの実をアルファ化して粉砕、篩別して、普通にそば茶等に利用される区分とは異なる高タンパク質含有区分を分取し、これらに種々の微生物や酵素を組み合わせて発酵させ、生理活性物質の生起の有無、その量について比較し、鋭意研究を行った結果、高タンパク質含有区分に麹菌を作用させることにより前述の生理活性作用を有するGABAが顕著に増加することを見出し、本発明に到ったものである。 Foods and drinks using buckwheat berries as raw materials contain abundant nutrients such as protein, vitamins, and minerals, and protein contains 10 to 15% by weight per 100 g of dry buckwheat. Tartary buckwheat contains a large amount of rutin, which is effective in lowering blood pressure, and conventional buckwheat tea obtained by roasting buckwheat raw materials in a normal manner has these actions, its aroma, and flavor. However, recently, there are techniques to ferment with buckwheat berries using microorganisms to remove buckwheat allergen protein, increase amino acids, etc., but physiological activities that can be used effectively The amount of substance produced was not yet sufficient. The present inventor has proceeded with research, alphalyzes the buckwheat, pulverizes and sifts, and sorts a high protein content category different from the category normally used for buckwheat tea, etc. As a result of comparing the presence or absence of physiologically active substances and their amounts, and conducting intensive research, GABA with the above-mentioned physiologically active effects is markedly increased by causing koji molds to act on high protein content categories. The present invention has been found and achieved.
すなわち本発明は、(1)ソバを原料としたγ−アミノ酪酸の含量が増強された飲食品の製造方法であって、ソバの種子の殻を取り除いて得たソバの実、もしくはソバの種子を蒸熱してアルファー化し、殻を取り除いて得たソバの実を粉砕し、少なくとも20重量%(乾燥基準)以上のタンパク質を含有する高タンパク質区分を分取し、この高タンパク質区分の粉砕物に麹菌を接種、培養して麹化させ、焙煎又は蒸熱することを特徴とするソバを原料とした飲食品の製造方法に関する。 That is, the present invention is (1) a method for producing a food or drink with an increased content of γ-aminobutyric acid using buckwheat as a raw material, wherein buckwheat seeds obtained by removing buckwheat seed shells or buckwheat seeds Steamed and alphatized, crushed buckwheat berries obtained by removing the shell, fractionated high protein section containing at least 20% by weight (dry basis) of protein, and pulverized the high protein section The present invention relates to a method for producing a food or drink using buckwheat as a raw material, characterized by inoculating, culturing, and hatching koji mold, roasting or steaming.
また本発明は、(2)高タンパク質区分が、ソバ粉砕物の乾燥基準で少なくとも30重量%以上のタンパク質を含有することを特徴とする上記(1)記載のソバを原料とした飲食品の製造方法記載のソバを原料とした飲食品の製造方法や、(3)ソバが、可食性ソバ(普通ソバ、ダッタンソバ、宿根ソバ等)であることを特徴とする上記(1)又は(2)記載のソバを原料とした飲食品の製造方法や、(4)飲食品が、茶飲料であることを特徴とする上記(1)〜(3)のいずれか記載のソバを原料とした飲食品の製造方法に関する。 The present invention also provides (2) the production of food and drink using buckwheat as a raw material according to (1) above, wherein the high protein classification contains at least 30% by weight or more of protein based on the dryness of the buckwheat pulverized product. (1) or (2) described above, wherein the method for producing a food or drink using the buckwheat as a raw material, or (3) the buckwheat is edible buckwheat (ordinary buckwheat, tartary buckwheat, perennial buckwheat, etc.) A method for producing a food or drink using buckwheat as a raw material, or (4) a food or drink using the buckwheat as a raw material according to any one of (1) to (3) above, wherein the food or drink is a tea drink It relates to a manufacturing method.
さらに本発明は、(5)上記(1)〜(4)のいずれか記載の飲食品の製造方法により得られるソバを原料とする飲食品に関する。 Furthermore, this invention relates to the food-drinks which use the buckwheat obtained by the manufacturing method of the food-drinks in any one of (5) said (1)-(4) as a raw material.
本発明によれば、従来のソバ飲食品と同様に、タンパク質、ビタミン、ミネラル等の栄養素を豊富に含み、かつこれらの栄養素を殆ど損なうことなく、従来のソバ飲食品と比べ、GABAを4〜15倍という高含量のソバ飲食品を得ることができる。 According to the present invention, like conventional buckwheat foods and drinks, GABA is contained in 4 to 4 in comparison with conventional buckwheat foods and drinks, containing abundant nutrients such as proteins, vitamins, minerals and the like, and hardly damaging these nutrients. Buckwheat food and drink with a high content of 15 times can be obtained.
本発明のソバを原料としたGABAの含量が増強された飲食品の製造方法としては、ソバを原料としたγ−アミノ酪酸の含量が増強された飲食品の製造方法であって、ソバの種子の殻を取り除いて得たソバの実、もしくはソバの種子を蒸熱してアルファー化し、殻を取り除いて得たソバの実を粉砕し、少なくとも20重量%(乾燥基準)以上のタンパク質を含有する高タンパク質区分を分取し、この高タンパク質区分の粉砕物に麹菌を接種、培養して麹化させ、焙煎又は蒸熱する方法であれば、特に制限されるものではなく、ソバ原料としては、通常の日本ソバ(Fagopyrum esculemtum)や苦ソバ(Fagopyrum tararicum ダッタンソバ)、宿根ソバ等の可食性ソバを用いることができる。 A method for producing a food or drink with an increased GABA content using buckwheat as a raw material is a method for producing a food or drink with an increased content of γ-aminobutyric acid using buckwheat as a raw material. The buckwheat berries obtained by removing the shells of the buckwheat, or the buckwheat seeds of the buckwheat, are alphalated by steaming, and the buckwheat berries obtained by removing the husks are crushed and contain at least 20% by weight (dry basis) or more protein. It is not particularly limited as long as it is a method of separating the protein section, inoculating koji mold on the pulverized product of this high protein section, culturing and hatching, roasting or steaming, Edible buckwheat such as Japanese buckwheat (Fagopyrum esculemtum), bitter buckwheat (Fagopyrum tararicum tartary buckwheat) and perennial buckwheat can be used.
そして、これらソバの実の少なくとも20重量%(乾燥基準)以上のタンパク質を含有する高タンパク質区分は、例えば、精選して夾雑物を除去し、整粒をおこなったソバ種子の殻を取り除いて得たソバの実や、精選して夾雑物を除去し、整粒をおこなったソバ種子を水に浸漬して湿潤させ(加水量30〜60%)、水を切った後に放置してソバ種子の内部まで水分を浸透させた後、蒸煮してアルファー化させ、そのソバ種子を乾燥した後ソバ種子の殻を取り除いて得たソバの実を粉砕し、得られるソバの実の粉砕物のうち、篩別してソバの実外層の高タンパク質含有区分を分取することにより得ることができる。高タンパク質含有区分としては、乾燥基準でタンパク質含量が20重量%以上、好ましくは25重量%以上、特に好ましくは30重量%以上含有するものを用いることができ、これらは、ソバの実、好ましくはアルファー化したソバの実を粉砕し、20メッシュスルー画分、好ましくは40メッシュスルー画分、より好ましくは50メッシュスルー画分の穀粉を分離することにより得られる。 And the high protein classification containing at least 20% by weight (dry basis) of protein of these buckwheat berries is obtained by, for example, selecting and removing impurities and removing the sized buckwheat seed shells. The buckwheat seeds and fine impurities are carefully removed to adjust the size of the buckwheat seed soaked in water (wet water 30-60%), drained and left to stand. After infiltrating moisture to the inside, steamed to alphalize, dry the buckwheat seeds, pulverize the buckwheat seeds obtained by removing the buckwheat seed shell, among the pulverized buckwheat seeds obtained, It can be obtained by sieving and separating the high protein content section of the outer buckwheat layer. As the high protein content category, those containing a protein content of 20% by weight or more, preferably 25% by weight or more, particularly preferably 30% by weight or more on a dry basis can be used. It is obtained by pulverizing alfalized buckwheat and separating flour of a 20 mesh-through fraction, preferably a 40 mesh-through fraction, more preferably a 50 mesh-through fraction.
本発明で用いる麹菌としては特に制限されるものではないが、味噌用麹、醤油用麹、もろみ用麹、もと用麹など一般にこの種の製造に使われている麹菌である、アスペルギルス・オリゼ(Aspergillus oryzae)、アスペルギルス・ソーヤ(Aspergillus soya)、アスペルギルス・ニガー(Aspergillus niger)等を用いることができる。 Aspergillus oryzae used in the present invention is not particularly limited, but Aspergillus oryzae is a gonococcus commonly used in this type of production, such as miso, soy sauce, moromi, and mash. (Aspergillus oryzae), Aspergillus soya, Aspergillus niger and the like can be used.
本発明の原料のソバの実を用いて麹化する条件としては、原料重量に対して0.05〜5重量%の量、好ましくは0.5〜2重量%の麹菌を播種し、十分に攪拌する。温度20〜35℃、好ましくは、25〜30℃、湿度70〜100%、好ましくは湿度80〜98%で20〜60時間、好ましくは30〜50時間培養し、ソバの実を麹化させることが好ましい。なお、麹化するときには熱が発生する為、10〜15時間おきに攪拌を行うことが好ましい。麹化された原料のソバの実を80〜140℃、好ましくは100〜130℃で3〜12分、好ましくは5〜7分間焙煎をするか、もしくは5〜20分間、好ましくは8〜15分間蒸熱を行うことが望ましい。 As conditions for hatching using the raw buckwheat berries of the present invention, 0.05 to 5% by weight, preferably 0.5 to 2% by weight of koji molds are inoculated with respect to the weight of the raw material. Stir. Incubate at a temperature of 20 to 35 ° C., preferably 25 to 30 ° C., a humidity of 70 to 100%, preferably a humidity of 80 to 98% for 20 to 60 hours, preferably 30 to 50 hours to hatch the buckwheat berries. Is preferred. In addition, since heat generate | occur | produces when hatching, it is preferable to stir every 10 to 15 hours. The hatched raw buckwheat berries are roasted at 80-140 ° C., preferably 100-130 ° C. for 3-12 minutes, preferably 5-7 minutes, or 5-20 minutes, preferably 8-15 It is desirable to steam for a minute.
本発明の飲食品としては、ソバを原料として通常のお茶と同じように飲む茶飲料、或いはふりかけ食品、スープ、さらには菓子類、乳飲料の製造原料として飲食できる飲食品を含む。 The foods and drinks of the present invention include tea drinks made from buckwheat in the same manner as ordinary tea, or foods and drinks that can be eaten and consumed as production raw materials for sprinkled foods, soups, confectionery, and dairy drinks.
本発明のソバを原料とした飲食品の製造方法の好ましい態様について、具体的に以下に例示する。まず、精選して夾雑物を除去し、整粒をおこなったソバ種子を水に浸漬して湿潤させ、水を切った後に放置してソバ種子の内部まで水分を浸透させる。このソバ種子を蒸煮してアルファー化させ、そのソバ種子を乾燥した後ソバ種子の殻を取り除く。殻を取り除いたソバの実を10メッシュスルーの大きさに製粉機等で粉砕する。次いで、粉砕物を篩にかけ、例えば、50メッシュスルーの粉砕物を分取する。この粉砕物は、通常、乾燥基準でタンパク質含量が30重量%以上となっている。この粉砕物に所定量の水を加え、一定時間後、蒸熱処理を行う。その後蒸熱処理物の温度が低下したところで、原料重量に対して0.05〜5重量%の量の麹菌を播種し、十分に攪拌する。温度20〜35℃、湿度70〜100%の条件で20〜60時間培養し、ソバの実を麹化させる。以上のようにして得られる発酵した高タンパク含有区分のソバの実を原料に用いた飲食品は、従来の発酵しないソバの実を原料に用いた飲食品や、麹化させたソバの実の全量粉砕物(タンパク質約12重量%含有)を原料に用いた飲食品と比べ、GABA含有量が増強され、ソバ本来の風味に加えて、香ばしい香り、風味も付加されている。 The preferable aspect of the manufacturing method of the food-drinks which use the buckwheat of this invention as a raw material is specifically illustrated below. First, impurities are carefully selected to remove impurities, and the adjusted buckwheat seeds are dipped in water to be moistened. After draining, the water is allowed to penetrate into the buckwheat seeds. The buckwheat seed is steamed to make it alpha, the buckwheat seed is dried, and then the buckwheat seed shell is removed. The buckwheat nuts from which the shell has been removed are pulverized to a size of 10 mesh through with a mill. Next, the pulverized product is sieved and, for example, 50 mesh-through pulverized product is collected. This pulverized product usually has a protein content of 30% by weight or more on a dry basis. A predetermined amount of water is added to the pulverized product, and after a certain time, steaming is performed. Thereafter, when the temperature of the steamed product is lowered, 0.05 to 5% by weight of the koji mold is inoculated with respect to the weight of the raw material and sufficiently stirred. It is cultured for 20 to 60 hours under conditions of a temperature of 20 to 35 ° C. and a humidity of 70 to 100%, and the buckwheat berries are hatched. Food and drink using fermented buckwheat nuts of high protein content obtained as described above as raw materials, food and drink using conventional non-fermented buckwheat nuts as raw materials, and hatched buckwheat nuts Compared to foods and drinks using the entire amount of pulverized product (containing about 12% by weight of protein) as a raw material, the GABA content is enhanced, and in addition to the original flavor of buckwheat, a fragrant fragrance and flavor are also added.
以下、実施例により本発明をより具体的に説明するが、本発明の技術的範囲はこれらの例示に限定されるものではない。 EXAMPLES Hereinafter, although an Example demonstrates this invention more concretely, the technical scope of this invention is not limited to these illustrations.
本発明の実施例で製造される飲食物中のGABAの測定方法を、次の手順で行った。
1)試料5gに対して50mLの80%エタノールを加える。
2)80℃でインキュベートしながら、還流抽出20分間行う。
3)上清を100mLフラスコに移す。
4)上清を採取した後の沈殿に再び80%エタノールを20mL加える。
5)80℃でインキュベートしながら、還流抽出20分間行う。
6)上清を3)のフラスコに混入する。
7)上記4)〜6)の操作を再度繰り返し、計3回分の上清を採取する。
8)得られた上清をろ過した後、エバポレーターを用いて濃縮する。
9)0.02N−HCL 50mLに溶かす。この際、3〜4回に分けて溶かす。
10)0.45μmのフィルターをつけたシリンダを用いてろ過し、測定用試料とする。
11)得られた試料をアミノ酸分析装置(日立テクノロジーズ)にかけ、GABAを分析する。
The measuring method of GABA in the food and drink manufactured in the Example of this invention was performed in the following procedure.
1) Add 50 mL of 80% ethanol to 5 g of sample.
2) Perform reflux extraction for 20 minutes while incubating at 80 ° C.
3) Transfer the supernatant to a 100 mL flask.
4) Add 20 mL of 80% ethanol to the precipitate after collecting the supernatant.
5) Perform reflux extraction for 20 minutes while incubating at 80 ° C.
6) Mix the supernatant into the flask of 3).
7) Repeat the above operations 4) to 6) again, and collect a total of 3 supernatants.
8) The obtained supernatant is filtered and then concentrated using an evaporator.
9) Dissolve in 50 mL of 0.02N-HCL. At this time, dissolve in 3 to 4 times.
10) Filter using a cylinder with a 0.45 μm filter to make a sample for measurement.
11) The obtained sample is applied to an amino acid analyzer (Hitachi Technologies) and GABA is analyzed.
精選して夾雑物を除去し、整粒をおこなった普通ソバの種子を水に3時間以上浸漬して湿潤させ、水を切った後に16時間以上放置してソバ種子の内部まで水分を浸透させた。このソバ種子を40分間以上蒸煮してアルファー化させ(蒸煮後水分38〜42%)、ソバ種子を水分が13%になるまで乾燥した後ソバ種子の殻を取り除いた。次に殻を取り除いたソバの実を10メッシュスルーの大きさに製粉機(「DK−400脱皮機」東京製粉機製作所社製)で粉砕した。次に、この全量粉砕物(タンパク質約12重量%含有;比較区)から50メッシュスルーの高タンパク含有区分(タンパク質33重量%含有;試験区)を篩い分けにより分取した。これら全量粉砕物(比較区)と高タンパク含有区分(試験区)の各々に50重量%の水を加え、60分後に30分間蒸熱処理を行った。その後蒸熱処理物の温度が30〜40℃に低下したところで、1重量%のアスペルギルス・オリゼ(製品名「M−1」日本醸造工業社製)を播種し、十分に攪拌し、温度30℃、湿度95%の条件で48時間培養し、ソバの実を麹化させた。なお、麹化するときには熱が発生する為、12時間おきに攪拌を行った。2種類の麹化されたソバの実と、麹化していない全量粉砕物(対照区1)と高タンパク含有区分(対照区2)を120℃で7分間焙煎処理を行い、4種類の「ソバ茶」を得た。これら4種類の「ソバ茶」を各5g取り、それにお湯を500mL入れ1分間煮出して4種類の茶飲料を調製した。これら4種類の茶飲料中のGABA含量(mg/100g)の測定結果を表1に示す。また、官能検査の結果を表2に示す。茶飲料中のGABA含量は、麹化処理を施していない(未処理)の対照区1及び対照区2に比べて、麹化処理を施した比較区では5倍に、試験区では11倍に増加しており、その増加は高タンパク質含有区分を原料とした試験区で顕著であった。また、茶飲料を飲用をしたところ、比較区及び試験区は、麹化処理を施していない(未処理)の対照区1及び対照区2よりも甘みが強く、独特の風味をもつものであった。特に、試験区は香ばしい香りを有していた。 After carefully selecting and removing impurities, regular buckwheat seeds that have been sized are soaked in water for 3 hours or longer, drained, and allowed to stand for 16 hours or longer to allow moisture to penetrate into the buckwheat seeds. It was. This buckwheat seed was cooked for 40 minutes or longer to make it alpha (moisture content 38-42% after cooking), and the buckwheat seed was dried until the water content became 13%, and then the buckwheat seed shell was removed. Next, the buckwheat nuts from which the shell had been removed were pulverized to a size of 10 mesh through with a mill ("DK-400 molting machine" manufactured by Tokyo Flour Mill Co., Ltd.). Next, a 50-mesh-through high protein content section (containing 33 wt% protein; test section) was fractionated by sieving from this total pulverized product (containing about 12 weight% protein; comparative section). 50% by weight of water was added to each of these pulverized products (comparative section) and the high protein-containing section (test section), and after 60 minutes, steaming was performed for 30 minutes. After that, when the temperature of the steamed product was lowered to 30 to 40 ° C., 1% by weight of Aspergillus oryzae (product name “M-1” manufactured by Nippon Brewing Industry Co., Ltd.) was seeded and stirred sufficiently, and the temperature was 30 ° C. By culturing for 48 hours under the condition of 95% humidity, buckwheat seeds were hatched. In addition, since heat generate | occur | produces when hatching, it stirred every 12 hours. Two kinds of hatched buckwheat berries, whole unground hatched product (control group 1) and high protein content section (control group 2) were roasted at 120 ° C. for 7 minutes, and four kinds of “ Soba tea was obtained. 5 g of each of these 4 types of “soba tea” was taken, 500 mL of hot water was added thereto and boiled for 1 minute to prepare 4 types of tea beverages. Table 1 shows the measurement results of GABA content (mg / 100 g) in these four types of tea beverages. Table 2 shows the results of the sensory test. The GABA content in tea drinks is 5 times higher in the control group that has been hatched and 11 times higher in the test group than the control group 1 and control group 2 that have not been hatched (untreated). The increase was remarkable in the test section using the high protein content section as a raw material. In addition, when drinking tea beverages, the comparison group and the test group were sweeter and had a unique flavor than the control group 1 and control group 2 which were not hatched (untreated). It was. In particular, the test area had a fragrant scent.
普通ソバの実に代えてダッタンソバの実を用いる以外は実施例1と同様にして4種類の「ダッタンソバ茶」を得た。これら4種類の「ダッタンソバ茶」を各5g取り、それにお湯を500mL入れ1分間煮出して4種類の茶飲料を調製した。これら4種類の茶飲料中のGABA含量(mg/100g)の測定結果を表3に示す。また、官能検査の結果を表4に示す。茶飲料中のGABA含量は、麹化処理を施していない(未処理)の対照区1及び対照区2に比べて、麹化処理を施した比較区では4倍に、試験区では15倍に増加しており、その増加は高タンパク質含有区分を原料とした試験区で顕著であった。また、茶飲料を飲用をしたところ、比較区及び試験区は、麹化処理を施していない(未処理)の対照区1及び対照区2よりも甘みが強く、独特の風味をもつものであった。特に、試験区は香ばしい香りを有していた。 Four types of “tartary buckwheat tea” were obtained in the same manner as in Example 1 except that tartary buckwheat was used instead of ordinary buckwheat. 5 g of each of these 4 types of “Tartary buckwheat tea” was taken, 500 mL of hot water was added thereto and boiled for 1 minute to prepare 4 types of tea beverages. Table 3 shows the measurement results of GABA content (mg / 100 g) in these four types of tea beverages. Table 4 shows the results of the sensory test. The GABA content in tea drinks is 4 times higher in the control group that has been hatched and 15 times higher in the test group than in control group 1 and control group 2 that have not been hatched (untreated). The increase was remarkable in the test section using the high protein content section as a raw material. In addition, when drinking tea beverages, the comparison group and the test group were sweeter and had a unique flavor than the control group 1 and control group 2 which were not hatched (untreated). It was. In particular, the test area had a fragrant scent.
麹菌の種類によりGABAの増加が異なるかを調べるため、5種類の種麹[製品名「M−1」、「富士」、「MF−2」、「もと用」、(以上は、アスペルギルス・オリゼ)、製品名「ソーヤ」(アスペルギルス・ソーヤ)、いずれも日本醸造工業社製]を用いて、前記実施例1及び2と同様の方法により麹化させ、焙煎によりソバ茶及びダッタンソバ茶を得た。該「ソバ茶」及び「ダッタンソバ茶」を5g取り、それにお湯を500mL入れ1分間煮出して24種類の茶飲料を調製した。これら24種類の茶飲料中のGABA含量(mg/100g)の測定結果を表5に示す。茶飲料中のGABA含量は、麹化処理を施していない(未処理)の対照区1及び対照区2に比べて、普通ソバの実を用いて麹化処理を施した比較区では2.5〜5倍に、試験区では4〜11倍に増加しており、またダッタンソバの実を用いて麹化処理を施した比較区では3〜4倍に、試験区では5〜15倍に増加しており、その増加はいずれも高タンパク質含有区分を原料とした試験区で顕著であった。また、これら茶飲料を飲用をしたところ、特にM-1麹と富士麹を用いたソバ茶及びダッタンソバ茶が甘みと風味が優れていた。 In order to investigate whether the increase in GABA varies depending on the type of Aspergillus oryzae, five types of seed koji [product names “M-1”, “Fuji”, “MF-2”, “original use”, (the above is Aspergillus ・Orise), product name “Soya” (Aspergillus soya), both made by Nippon Brewing Industry Co., Ltd.], and hatched by the same method as in Examples 1 and 2, and buckwheat tea and tartary buckwheat tea were roasted. Obtained. Five grams of the “buckwheat tea” and “dattan buckwheat tea” were taken, 500 mL of hot water was added thereto and boiled for 1 minute to prepare 24 types of tea beverages. Table 5 shows the measurement results of GABA content (mg / 100 g) in these 24 types of tea beverages. The GABA content in the tea beverage was 2.5 in the comparative group that was hatched using normal buckwheat berries compared to the control group 1 and control group 2 that were not hatched (untreated). Increased to 5 to 5 times, increased to 4 to 11 times in the test area, increased to 3 to 4 times in the comparative area where hatching treatment was performed using tartary buckwheat, and increased to 5 to 15 times in the experimental area The increase was remarkable in the test plots using high protein content as the raw material. Moreover, when these tea drinks were drunk, especially the buckwheat tea and tartary buckwheat tea using M-1 rice cake and Fuji coffee were excellent in sweetness and flavor.
M-1麹を用いて麹化したソバの実あるいはダッタンソバの実のその後の処理方法によって茶飲料中のGABA含有量にどのような影響があるか、焙煎時間の長短、あるいは蒸熱処理の有無によるGABA含量の変化を測定した。焙煎処理を施すとGABAは低下し、その低下は焙煎時間が長くなるにつれ大きくなった。それに対し10分間の蒸熱処理は、焙煎処理よりもGABAの低下を抑えることが可能であった。焙煎時間によるGABA含量の変化を表6に、蒸熱処理によるGABA含量の変化を表7に記す。 How does the subsequent processing method of buckwheat or tartary buckwheat hatched with M-1 麹 affect the GABA content in tea beverages, the length of roasting time, or the presence or absence of steaming The change in GABA content due to was measured. When the roasting treatment was performed, GABA decreased, and the decrease became larger as the roasting time became longer. On the other hand, steam heat treatment for 10 minutes was able to suppress the decrease in GABA more than the roasting treatment. Table 6 shows changes in GABA content due to roasting time, and Table 7 shows changes in GABA content due to steaming.
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| CN104488537A (en) * | 2014-12-31 | 2015-04-08 | 吴中区金庭小美华家庭农场 | Planting method of tartary buckwheat rich in gamma-aminobutyric acid and dietary fibers |
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| CN111657471A (en) * | 2020-05-27 | 2020-09-15 | 安徽乐方食品有限公司 | Processing method of health nut meat rich in gamma-aminobutyric acid |
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