JP2008523828A5 - - Google Patents

Download PDF

Info

Publication number: JP2008523828A5
Authority: JP; Japan
Prior art keywords: library; probe; oligonucleotide; target; anthraquinone
Prior art date: 2005-12-21
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Withdrawn

Application number

JP2007547182A

Other languages

English (en)

Japanese (ja)

Other versions

JP2008523828A (ja

Filing date

2005-12-21

Publication date

2009-02-12

2005-12-21 Application filed filed Critical

2005-12-21 Priority claimed from PCT/DK2005/000815 external-priority patent/WO2006066592A2/fr

2008-07-10 Publication of JP2008523828A publication Critical patent/JP2008523828A/ja

2009-02-12 Publication of JP2008523828A5 publication Critical patent/JP2008523828A5/ja

Status Withdrawn legal-status Critical Current

Links

108020005187 Oligonucleotide Probes Proteins 0.000 claims description 46
239000002751 oligonucleotide probe Substances 0.000 claims description 46
239000000523 sample Substances 0.000 claims description 43
150000007523 nucleic acids Chemical class 0.000 claims description 27
108020004707 nucleic acids Proteins 0.000 claims description 20
102000039446 nucleic acids Human genes 0.000 claims description 20
238000000034 method Methods 0.000 claims description 13
108091034117 Oligonucleotide Proteins 0.000 claims description 11
238000001514 detection method Methods 0.000 claims description 10
230000000295 complement effect Effects 0.000 claims description 9
230000027455 binding Effects 0.000 claims description 6
241000282414 Homo sapiens Species 0.000 claims description 5
238000003556 assay Methods 0.000 claims description 5
238000002844 melting Methods 0.000 claims description 4
230000008018 melting Effects 0.000 claims description 4
239000000178 monomer Substances 0.000 claims description 4
108091028043 Nucleic acid sequence Proteins 0.000 claims description 3
230000009870 specific binding Effects 0.000 claims description 2
125000003729 nucleotide group Chemical group 0.000 claims 13
239000002773 nucleotide Substances 0.000 claims 12
-1 hydroxy, amino Chemical group 0.000 claims 7
KDCGOANMDULRCW-UHFFFAOYSA-N 7H-purine Chemical compound N1=CNC2=NC=NC2=C1 KDCGOANMDULRCW-UHFFFAOYSA-N 0.000 claims 6
125000002490 anilino group Chemical group [H]N(*)C1=C([H])C([H])=C([H])C([H])=C1[H] 0.000 claims 6
239000011230 binding agent Substances 0.000 claims 4
OZFPSOBLQZPIAV-UHFFFAOYSA-N 5-nitro-1h-indole Chemical compound [O-][N+](=O)C1=CC=C2NC=CC2=C1 OZFPSOBLQZPIAV-UHFFFAOYSA-N 0.000 claims 3
108020004414 DNA Proteins 0.000 claims 3
239000002299 complementary DNA Substances 0.000 claims 3
150000004985 diamines Chemical class 0.000 claims 3
239000012634 fragment Substances 0.000 claims 3
108020004999 messenger RNA Proteins 0.000 claims 3
239000007787 solid Substances 0.000 claims 3
238000006467 substitution reaction Methods 0.000 claims 3
ZEMGGZBWXRYJHK-UHFFFAOYSA-N thiouracil Chemical compound O=C1C=CNC(=S)N1 ZEMGGZBWXRYJHK-UHFFFAOYSA-N 0.000 claims 3
OQSYYTSWSXEJHW-UHFFFAOYSA-N 1,8-bis(3-hydroxypropylamino)anthracene-9,10-dione Chemical compound O=C1C2=CC=CC(NCCCO)=C2C(=O)C2=C1C=CC=C2NCCCO OQSYYTSWSXEJHW-UHFFFAOYSA-N 0.000 claims 2
101710163270 Nuclease Proteins 0.000 claims 2
JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 claims 2
238000009830 intercalation Methods 0.000 claims 2
239000000203 mixture Substances 0.000 claims 2
125000006308 propyl amino group Chemical group 0.000 claims 2
239000000126 substance Substances 0.000 claims 2
LQCXSTKAMNHLCH-UHFFFAOYSA-N 1,4-bis(3-hydroxypropylamino)-6-methylanthracene-9,10-dione Chemical compound C1=CC(NCCCO)=C2C(=O)C3=CC(C)=CC=C3C(=O)C2=C1NCCCO LQCXSTKAMNHLCH-UHFFFAOYSA-N 0.000 claims 1
LUYYUZYWBTWOFO-UHFFFAOYSA-N 1,4-bis(3-hydroxypropylamino)anthracene-9,10-dione Chemical compound O=C1C2=CC=CC=C2C(=O)C2=C1C(NCCCO)=CC=C2NCCCO LUYYUZYWBTWOFO-UHFFFAOYSA-N 0.000 claims 1
GMJKWGLQXRMWPW-UHFFFAOYSA-N 1,4-bis[4-(2-hydroxyethyl)anilino]anthracene-9,10-dione Chemical compound C1=CC(CCO)=CC=C1NC(C=1C(=O)C2=CC=CC=C2C(=O)C=11)=CC=C1NC1=CC=C(CCO)C=C1 GMJKWGLQXRMWPW-UHFFFAOYSA-N 0.000 claims 1
CFQZCRRDAULXRH-UHFFFAOYSA-N 1,5-bis(3-hydroxypropylamino)anthracene-9,10-dione Chemical compound O=C1C2=C(NCCCO)C=CC=C2C(=O)C2=C1C=CC=C2NCCCO CFQZCRRDAULXRH-UHFFFAOYSA-N 0.000 claims 1
JJZHVDFHKFWLIH-UHFFFAOYSA-N 1-[3-[bis(4-methoxyphenyl)-phenylmethoxy]propylamino]-4-(3-hydroxypropylamino)anthracene-9,10-dione Chemical compound C1=CC(OC)=CC=C1C(C=1C=CC(OC)=CC=1)(C=1C=CC=CC=1)OCCCNC1=CC=C(NCCCO)C2=C1C(=O)C1=CC=CC=C1C2=O JJZHVDFHKFWLIH-UHFFFAOYSA-N 0.000 claims 1
RBUHNGWCCHXPOS-UHFFFAOYSA-N 1-[3-[bis(4-methoxyphenyl)-phenylmethoxy]propylamino]-5-(3-hydroxypropylamino)anthracene-9,10-dione Chemical compound C1=CC(OC)=CC=C1C(C=1C=CC(OC)=CC=1)(C=1C=CC=CC=1)OCCCNC1=CC=CC2=C1C(=O)C1=CC=CC(NCCCO)=C1C2=O RBUHNGWCCHXPOS-UHFFFAOYSA-N 0.000 claims 1
QUNZQVRSTLNGHH-UHFFFAOYSA-N 1-[3-[bis(4-methoxyphenyl)-phenylmethoxy]propylamino]-8-(3-hydroxypropylamino)anthracene-9,10-dione Chemical compound C1=CC(OC)=CC=C1C(C=1C=CC(OC)=CC=1)(C=1C=CC=CC=1)OCCCNC1=CC=CC2=C1C(=O)C1=C(NCCCO)C=CC=C1C2=O QUNZQVRSTLNGHH-UHFFFAOYSA-N 0.000 claims 1
PRDFBSVERLRRMY-UHFFFAOYSA-N 2'-(4-ethoxyphenyl)-5-(4-methylpiperazin-1-yl)-2,5'-bibenzimidazole Chemical compound C1=CC(OCC)=CC=C1C1=NC2=CC=C(C=3NC4=CC(=CC=C4N=3)N3CCN(C)CC3)C=C2N1 PRDFBSVERLRRMY-UHFFFAOYSA-N 0.000 claims 1
ASJSAQIRZKANQN-CRCLSJGQSA-N 2-deoxy-D-ribose Chemical compound OC[C@@H](O)[C@@H](O)CC=O ASJSAQIRZKANQN-CRCLSJGQSA-N 0.000 claims 1
WQHGVTJIKDXENN-UHFFFAOYSA-N 3-(pyren-1-ylmethoxy)propane-1,2-diol Chemical compound C1=C2C(COCC(O)CO)=CC=C(C=C3)C2=C2C3=CC=CC2=C1 WQHGVTJIKDXENN-UHFFFAOYSA-N 0.000 claims 1
FWBHETKCLVMNFS-UHFFFAOYSA-N 4',6-Diamino-2-phenylindol Chemical compound C1=CC(C(=N)N)=CC=C1C1=CC2=CC=C(C(N)=N)C=C2N1 FWBHETKCLVMNFS-UHFFFAOYSA-N 0.000 claims 1
IOCHVQUKLRTZFT-UHFFFAOYSA-N 5,8-bis(4-methylanilino)-9,10-dioxoanthracene-2-carboxylic acid Chemical compound C1=CC(C)=CC=C1NC(C=1C(=O)C2=CC=C(C=C2C(=O)C=11)C(O)=O)=CC=C1NC1=CC=C(C)C=C1 IOCHVQUKLRTZFT-UHFFFAOYSA-N 0.000 claims 1
VXJVWRHUIAISNC-UHFFFAOYSA-N 9,10-dioxo-5,8-bis(propylamino)anthracene-2-carboxylic acid Chemical compound O=C1C2=CC(C(O)=O)=CC=C2C(=O)C2=C1C(NCCC)=CC=C2NCCC VXJVWRHUIAISNC-UHFFFAOYSA-N 0.000 claims 1
241000219194 Arabidopsis Species 0.000 claims 1
239000003155 DNA primer Substances 0.000 claims 1
241000255581 Drosophila <fruit fly, genus> Species 0.000 claims 1
241000699666 Mus <mouse, genus> Species 0.000 claims 1
241000244206 Nematoda Species 0.000 claims 1
229910019142 PO4 Inorganic materials 0.000 claims 1
241000282577 Pan troglodytes Species 0.000 claims 1
ZYFVNVRFVHJEIU-UHFFFAOYSA-N PicoGreen Chemical compound CN(C)CCCN(CCCN(C)C)C1=CC(=CC2=[N+](C3=CC=CC=C3S2)C)C2=CC=CC=C2N1C1=CC=CC=C1 ZYFVNVRFVHJEIU-UHFFFAOYSA-N 0.000 claims 1
241000700159 Rattus Species 0.000 claims 1
CGNLCCVKSWNSDG-UHFFFAOYSA-N SYBR Green I Chemical compound CN(C)CCCN(CCC)C1=CC(C=C2N(C3=CC=CC=C3S2)C)=C2C=CC=CC2=[N+]1C1=CC=CC=C1 CGNLCCVKSWNSDG-UHFFFAOYSA-N 0.000 claims 1
125000003545 alkoxy group Chemical group 0.000 claims 1
125000000217 alkyl group Chemical group 0.000 claims 1
125000005600 alkyl phosphonate group Chemical group 0.000 claims 1
125000003277 amino group Chemical group 0.000 claims 1
230000003321 amplification Effects 0.000 claims 1
125000003710 aryl alkyl group Chemical group 0.000 claims 1
125000003118 aryl group Chemical group 0.000 claims 1
NAGJZTKCGNOGPW-UHFFFAOYSA-K dioxido-sulfanylidene-sulfido-$l^{5}-phosphane Chemical compound [O-]P([O-])([S-])=S NAGJZTKCGNOGPW-UHFFFAOYSA-K 0.000 claims 1
201000010099 disease Diseases 0.000 claims 1
208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims 1
230000009977 dual effect Effects 0.000 claims 1
239000000975 dye Substances 0.000 claims 1
ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 claims 1
229960005542 ethidium bromide Drugs 0.000 claims 1
PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims 1
239000010931 gold Substances 0.000 claims 1
229910052737 gold Inorganic materials 0.000 claims 1
229910052739 hydrogen Inorganic materials 0.000 claims 1
239000001257 hydrogen Substances 0.000 claims 1
125000004435 hydrogen atom Chemical class [H]* 0.000 claims 1
238000012986 modification Methods 0.000 claims 1
230000004048 modification Effects 0.000 claims 1
238000003199 nucleic acid amplification method Methods 0.000 claims 1
NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims 1
239000010452 phosphate Substances 0.000 claims 1
150000004713 phosphodiesters Chemical class 0.000 claims 1
PTMHPRAIXMAOOB-UHFFFAOYSA-L phosphoramidate Chemical compound NP([O-])([O-])=O PTMHPRAIXMAOOB-UHFFFAOYSA-L 0.000 claims 1
INAAIJLSXJJHOZ-UHFFFAOYSA-N pibenzimol Chemical compound C1CN(C)CCN1C1=CC=C(N=C(N2)C=3C=C4NC(=NC4=CC=3)C=3C=CC(O)=CC=3)C2=C1 INAAIJLSXJJHOZ-UHFFFAOYSA-N 0.000 claims 1
125000000548 ribosyl group Chemical class C1([C@H](O)[C@H](O)[C@H](O1)CO)* 0.000 claims 1
ACOJCCLIDPZYJC-UHFFFAOYSA-M thiazole orange Chemical compound CC1=CC=C(S([O-])(=O)=O)C=C1.C1=CC=C2C(C=C3N(C4=CC=CC=C4S3)C)=CC=[N+](C)C2=C1 ACOJCCLIDPZYJC-UHFFFAOYSA-M 0.000 claims 1
ANRHNWWPFJCPAZ-UHFFFAOYSA-M thionine Chemical compound [Cl-].C1=CC(N)=CC2=[S+]C3=CC(N)=CC=C3N=C21 ANRHNWWPFJCPAZ-UHFFFAOYSA-M 0.000 claims 1
RYYWUUFWQRZTIU-UHFFFAOYSA-K thiophosphate Chemical compound [O-]P([O-])([O-])=S RYYWUUFWQRZTIU-UHFFFAOYSA-K 0.000 claims 1
150000005691 triesters Chemical class 0.000 claims 1
241000894006 Bacteria Species 0.000 description 2
238000003753 real-time PCR Methods 0.000 description 2
0 *c(c(*)c1C(c2c(*)c(*)c(*)c(*)c22)=O)c(*)c(*)c1C2=O Chemical compound *c(c(*)c1C(c2c(*)c(*)c(*)c(*)c22)=O)c(*)c(*)c1C2=O 0.000 description 1
108091027075 5S-rRNA precursor Proteins 0.000 description 1
GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 1
229940124276 oligodeoxyribonucleotide Drugs 0.000 description 1
108090000623 proteins and genes Proteins 0.000 description 1

JP2007547182A 2004-12-22 2005-12-21 核酸混合物を解析するためのプローブ、ライブラリーおよびキット、並びにその構築方法 Withdrawn JP2008523828A (ja)

Applications Claiming Priority (4)

Application Number	Priority Date	Filing Date	Title
US63785704P	2004-12-22	2004-12-22
DKPA200401987		2004-12-22
DKPA200402012		2004-12-28
PCT/DK2005/000815 WO2006066592A2 (fr)	2004-12-22	2005-12-21	Sondes, bibliotheques et trousses pour l'analyse de melanges d'acides nucleiques et leurs procedes de construction

Publications (2)

Publication Number	Publication Date
JP2008523828A JP2008523828A (ja)	2008-07-10
JP2008523828A5 true JP2008523828A5 (fr)	2009-02-12

Family

ID=36292652

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
JP2007547182A Withdrawn JP2008523828A (ja)	2004-12-22	2005-12-21	核酸混合物を解析するためのプローブ、ライブラリーおよびキット、並びにその構築方法

Country Status (4)

Country	Link
EP (1)	EP1831394A2 (fr)
JP (1)	JP2008523828A (fr)
CA (1)	CA2593916A1 (fr)
WO (1)	WO2006066592A2 (fr)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
AU2010240092A1 (en) *	2009-04-22	2011-12-08	Vertex Pharmaceuticals Incorporated	Probe set for identification of nucleotide mutation, and method for identification of nucleotide mutation

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
AU2003260292A1 (en) *	2002-09-11	2004-04-30	Exiqon A/S	A population of nucleic acids including a subpopulation of lna oligomers
CA2498320A1 (fr) *	2002-09-20	2004-04-01	Integrated Dna Technologies, Inc.	Colorants extincteurs de fluorescence a base d'anthraquinone et procedes de fabrication et d'utilisation
EP1639130B1 (fr) *	2003-06-20	2012-04-11	Exiqon A/S	Sondes, bibliotheques et trousses d'analyse pour melanges d'acides nucleiques et methodes de realisation

2005
- 2005-12-21 WO PCT/DK2005/000815 patent/WO2006066592A2/fr not_active Ceased
- 2005-12-21 CA CA002593916A patent/CA2593916A1/fr not_active Abandoned
- 2005-12-21 JP JP2007547182A patent/JP2008523828A/ja not_active Withdrawn
- 2005-12-21 EP EP05804988A patent/EP1831394A2/fr not_active Withdrawn

Publication	Publication Date	Title
US11111535B2 (en)	2021-09-07	Probes, libraries and kits for analysis of mixtures of nucleic acids and methods for constructing the same
EP2765202B1 (fr)	2017-02-01	Procédés de capture de cible non spécifique d'acides nucléiques
CA2572472C (fr)	2013-08-13	Methodes et compositions de detection d'acides nucleiques dans un echantillon biologique
KR20220044486A (ko)	2022-04-08	샘플 내 분석 물질의 신호 인코딩 방법
CN1961081B (zh)	2012-09-05	用于定量微rna和小干扰rna的新方法
CZ20023749A3 (cs)	2003-04-16	Způsob detekce a pár sond tvořených nukleovou kyselinou
CA2744004A1 (fr)	2010-06-17	Compositions et procedes pour detecter des petits arn et utilisations de ceux-ci
US20180127803A1 (en)	2018-05-10	Ultra sensitive probes for detection of nucleic acid
KR20090071641A (ko)	2009-07-01	약 ８ 내지 ５０개 뉴클레오티드 길이의 짧은 핵산 서열의 정성적 및 정량적 검출을 위한 신규 방법
CN101573454A (zh)	2009-11-04	用于荧光原位杂交和芯片技术的核酸信标
WO2004074480A1 (fr)	2004-09-02	Procede d'amplification de signal pour detecter un gene mutant
RU2005100511A (ru)	2005-08-27	Идентификация олигонуклеотидов для захвата, выявления и количественного определения нуклеиновой кислоты вируса гепатита а
KR100763906B1 (ko)	2007-10-05	9 종의 박테리아의 표적 서열을 특이적으로 증폭할 수 있는 프라이머 세트 및 상기 9 종의 박테리아 중 각 표적 서열에 특이적으로 혼성화하는 프로브 올리고뉴클레오티드
JP2008523828A5 (fr)	2009-02-12
US20200087726A1 (en)	2020-03-19	Ultra sensitive probes for detection of nucleic acid
CN101090979A (zh)	2007-12-19	用于分析核酸混合物的探针、文库和试剂盒及其构建方法
JPWO2004072302A1 (ja)	2006-06-01	発現遺伝子検出のためのシグナル増幅方法
JP4573833B2 (ja)	2010-11-04	核酸混合物を解析するためのプローブ、ライブラリーおよびキット、並びにその構築方法
WO2015071552A1 (fr)	2015-05-21	Sondes à plusieurs motifs présentant une spécificité élevée et procédé de conception de ces dernières
US20060166238A1 (en)	2006-07-27	Probes, libraries and kits for analysis of mixtures of nucleic acids and methods for constructing the same
KR100663992B1 (ko)	2007-01-02	인유두종 바이러스의 유전형을 분석하기 위한 특이도 높은 프로브 선택방법 및 그 선택된 프로브
US20060014183A1 (en)	2006-01-19	Extendable probes
JP2021532787A5 (fr)	2024-01-16
JP2008534011A (ja)	2008-08-28	ユニーク配列ハイブリダイゼーションプローブ（ｕｓｐ）
JP2010110235A (ja)	2010-05-20	核酸プローブを用いた核酸の変異検出方法