JP2017201906A - Nutrient composition and dietary supplement - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide 3HB in such a form that facilitates oral administration, reduces an adverse effect on human bodies, and is available as a good energy source with immediate effectivity.SOLUTION: A nutrient composition contains a basic amino acid salt of 3HB (3 hydroxybutyric acid monomer) as a main component.SELECTED DRAWING: None

Description

  The present invention relates to a nutrient composition and a dietary supplement.

  Since 3-hydroxybutyric acid and its salt (hereinafter referred to as 3HB, also referred to as 3HB, especially its monomer) is a substance that is originally present in the human body, it has high biocompatibility and is a sugar. Expected to be a groundbreaking energy source instead of quality. In addition, it has been found that 3HB not only serves as a mere energy source but also acts as a signal transmitter that affects the expression of various genes and the activity of proteins. 3HB is known to improve cognitive function and long-lasting memory by inhibiting histone deacetylase, for example, by regulating gene expression, and its effectiveness in preventing Alzheimer's has been confirmed. For example, 3HB produced by ingestion and metabolism in the body of medium chain fatty acids that are abundant in coconut oil has the effect of improving the symptoms of Alzheimer's disease and diabetic patients who cannot utilize carbohydrates in the brain and body. It has been known. In addition, 3HB is converted to energy more rapidly than carbohydrates in the body and has the effect of suppressing the absorption of fat and sugar into cells, so it can be applied to energy substances for athletes, diet and health foods. Can be expected.

  Such 3HB can be obtained by causing various microorganisms to produce poly-3-hydroxybutyric acid (hereinafter referred to as PHB) and then decomposing the obtained PHB with an enzyme or the like (Patent Document 1).

  As a form in which 3HB can be administered to humans, it is known that 3HB can be administered intravenously as an oligomer (Patent Document 2).

JP 2010-168595 A Japanese Patent Laid-Open No. 06-321778

However, since 3HB is acidic, it is highly resistant to human ingestion, and it is difficult to ingest large amounts as it is. In addition, in order to reduce acidity, it may be taken as a metal salt, but in such a case, there is concern about excessive intake of the metal component, and excessive intake of the sodium component in particular may cause hypertension or liver disease. Therefore, even if you can take it orally, you want to avoid taking a large amount.
In addition, when administered intravenously as an oligomer, it is more invasive than oral administration, and is much more resistant to human consumption. Further, although the oligomer is an amino acid salt, the oligomer is metabolized while being decomposed. The process is very slow and lacks the immediate effect on energy conversion of 3HB, so that there is a drawback that the application is greatly limited.

  Therefore, in view of the above-mentioned circumstances, the present invention makes it easy to orally administer 3HB, suppresses adverse effects on the human body, and can be provided in a form that can be used as an excellent and effective energy source. The point is to provide food.

  The characteristic composition of the nutrient composition of the present invention for achieving the above object is that it contains a basic amino acid salt of 3HB (3-hydroxybutyric acid monomer) as a main component.

  According to the above configuration, 3HB is in a form neutralized with a basic amino acid and has little acidity even when ingested directly, so that it is difficult for a person to take great resistance when ingested orally. Moreover, since it is not a metal salt, there is no problem of excessive intake of metal components (for example, sodium). In addition, since it is known that the basic amino acid produced by the decomposition of 3HB basic amino acid salt in the body exhibits a good effect on the human body, both 3HB and the basic amino acid have the effects on the human body. Therefore, it is considered preferable. The basic amino acid salt of 3HB has a low molecular weight because it is a salt of a monomer of 3-hydroxybutyric acid and a basic amino acid. Compared to the use of oligomers or polymers, the decomposition rate is comparative. It has been experimentally clarified that blood ketone levels can be increased in a short time even when taken orally.

  As a preferable effect of the basic amino acid, for example, when the nutrient composition contains a 3HB arginine derivative salt as a main component, an effect of improving blood flow can be expected. In addition, since such a basic amino acid itself has a high pH, it is highly resistant to oral ingestion, but there is an advantage that the intake resistance can be suppressed synergistically by using a salt of 3HB.

  Arginine derivatives as basic amino acids include arginine and citrulline, and other basic amino acids include histidine and lysine.

  Therefore, the nutrient composition of the present invention can be used as an excellent energy source with immediate effect and can be easily ingested in a sufficient amount. Therefore, 3HB can be used as a dietary supplement exemplified by health supplements such as powders, granules, tablets and tablets, and functional foods such as cookies, biscuits, gummi, chocolate and gum. The availability of 3HB can be greatly increased.

Graph showing results of oral intake test

  Below, the nutrient composition and dietary supplement concerning embodiment of this invention are demonstrated. Preferred embodiments are described below, but these embodiments are described in order to more specifically illustrate the present invention, and various modifications can be made without departing from the spirit of the present invention. The present invention is not limited to the following description.

[3HB]
3HB is obtained by performing a fermentation process to which 3HB-producing halomonas are added, separating and removing halomonas from the resulting fermentation broth, and purifying. The fermentation process is a process in which 3HB-producing halomonas bacteria are added as they are to a raw material liquid containing a saccharide nutrient source such as fruit juice, and aerobic fermentation and anaerobic fermentation are performed in this order (see JP2013-081403A). Can be implemented as As a result, the sugar is converted to 3HB and produced in the fermentation broth.

[3HB arginine salt]
3HB arginine salt (an example of 3HB arginine derivative salt) as a basic amino acid salt of 3HB is neutralized to neutralize a fermentation solution whose pH decreases with the formation of 3HB in the step of anaerobic fermentation in the 3HB production process. By using arginine as an agent, it can be produced as a fermentation broth dissolved in the form of 3HB arginine salt (the anaerobic fermentation process is completed in 4 to 8 hours in alkaline anaerobic fermentation). A supernatant containing about 10% of 3HB arginine salt is separated by removing the cells by membrane separation, and the fermentation liquid is purified as a powdered 3HB arginine salt by distilling off the solvent. Is done. Alternatively, calcium hydroxide is used as a neutralizing agent to neutralize the fermentation solution whose pH decreases with the formation of 3HB, and after removing the cells by membrane separation after anaerobic fermentation, sulfuric acid is added to the calcium sulfate salt. Is purified as a powdery 3HB arginine salt by adding arginine to the precipitate removed and distilling off the solvent.

[Nutrient composition]
The nutritional composition according to this embodiment can be obtained by directly tableting the obtained powdered 3HB arginine salt and providing it as a nutritional supplement or a dietary supplement containing the nutritional composition. Moreover, in tableting, the addition of other components such as a preparation aid is not prevented. Moreover, when providing as a dietary supplement, it can process with other food components, such as flour and sugar, and can be made into forms, such as a cookie, a biscuit, gummy, chocolate, gum.

[Ingestion test]
Converted to 15 g per 60 kg body weight, 3HB arginine salt was orally ingested before a meal to a person to be monitored, and the 3HB concentration in blood was measured over time. As a result, as shown in FIG. 1, it reached 0.6 mM after 30 minutes and reached 1 mM after 45 minutes, and the blood concentration of 3HB increased in a short time after oral intake. In Patent Document 2, it reached 700 μmol / L after 30 minutes in rat blood, but when converted to human, it corresponds to 120 minutes later, and it takes time to increase the blood concentration, but the blood concentration is very quickly. It was found that the effect increased immediately.

[Sensory test]
(Method)
For arginine (commercial product), 3HB (purified product), and 3HB arginine salt (above examples), the ranking method (1 sourness, bitterness, and umami was felt with little bitterness, bitterness, and umami, bitterness, sourness) Evaluation was made on the basis of a 4-level display (4). 3HB was used as a final 3HBNa salt recovered using NaOH for pH adjustment in the conventional manner, desalted by electrodialysis and ion exchange, purified and isolated.
Arginine, 3HB, and 3HB arginine salts were weighed 100 mg each and dissolved in 1 ml of water, and each panelist conducted a sensory test for bitterness, sourness and umami.

(result)
The sensory test result regarding Table 1 (bitterness), Table 2 (acidity), and Table 3 (umami) is shown. Based on the results of Table 1, Table 2, and Table 3, bitterness, sourness, and taste were evaluated. Arginine has no acidity but high bitterness, while 3HB has no bitterness but high acidity. However, it was found that 3HB arginine salt did not feel sour and bitter, but a result of feeling umami was obtained, a clear significant difference was observed, and it was easy to eat.

3HB has been recognized as an effective energy substance for Alzheimer's prevention, cancer prevention, and athletes, but now it must be ingested as a 3HB salt or ester. With 3HB metal salt, too much metal component is taken, and ester has a complicated manufacturing process and requires enzymatic degradation in the body, so that it is poor in immediate effect. In the present invention, since it can be ingested as an amino acid salt, it is easy to ingest without taking too much metal component (especially sodium). Moreover, it can be divided into 3HB and amino acids in the body, and the effects can be expected. For example, the basic amino acid arginine has a blood flow improving function.
Furthermore, arginine is known to have an action of expanding blood vessels by increasing nitric oxide when ingested. Nitric oxide is used to reduce active oxygen in the presence of active oxygen, and its effect disappears. Therefore, when ingesting arginine, simultaneous inoculation with an antioxidant (reducing agent) is preferable.
In addition, 3HB is converted into acetoacetic acid in the body, and at that time, NAD which is a coenzyme is required, and NADH can be produced. NADH has a reducing power, and when taken as 3HB arginine, it suppresses the oxidation of arginine and can enhance the vasodilatory effect as compared with arginine alone, and is considered to have a synergistic effect.

[Another embodiment]
Other embodiments described below can be applied to the above embodiments in combination, as long as no contradiction arises. The scope of the present invention is not limited to these embodiments.

(1)
In the above embodiment, arginine salt was produced by using arginine as a neutralizing agent in the production process by 3HB fermentation process, but not limited thereto, purified 3HB is reacted with arginine to form 3HB arginine. Alternatively, 3HB arginine can also be obtained by reacting with arginine sulfate after making 3HB calcium salt using calcium hydroxide as a neutralizing agent. In any case, 3HB arginine can be produced by a simple method, which is preferable.

  In the above example, 3HB arginine is exemplified, but other basic amino acid salts may be used. In such a case, instead of arginine in the above embodiment, citrulline as another basic amino acid, It is clear that 3HB citrulline, 3HB histidine, and 3HB lysine can be obtained by using histidine and lysine. Similar to 3HB arginine, the acidity of 3HB is reduced, resistance to oral ingestion is less likely, and the unique effects of 3HB and citrulline, histidine, and lysine can be expressed simultaneously. Expected to be effective.

  The nutritional composition of the present invention can be used as an excellent energy source with immediate effect and can be easily taken orally, so health supplements such as powders, granules, tablets, tablets, It can be used by being added to a dietary supplement exemplified by functional foods such as cookies, biscuits, gummi, chocolate, and gum, and the availability of 3HB can be greatly increased.

Claims (3)

  A nutritional composition containing a basic amino acid salt of 3HB (3-hydroxybutyric acid monomer) as a main component.   A nutrient composition containing an arginine derivative salt of 3HB (3-hydroxybutyric acid monomer) as a main component.   A dietary supplement containing the nutrient composition according to claim 1 or 2.

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