JP3473228B2 - Orchid cultivation promoter - Google Patents
Orchid cultivation promoterInfo
- Publication number
- JP3473228B2 JP3473228B2 JP31610795A JP31610795A JP3473228B2 JP 3473228 B2 JP3473228 B2 JP 3473228B2 JP 31610795 A JP31610795 A JP 31610795A JP 31610795 A JP31610795 A JP 31610795A JP 3473228 B2 JP3473228 B2 JP 3473228B2
- Authority
- JP
- Japan
- Prior art keywords
- orchid
- cultivation
- orchid cultivation
- pseudomonas
- medium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 241000233855 Orchidaceae Species 0.000 title claims description 16
- 230000001737 promoting effect Effects 0.000 claims description 15
- 244000005700 microbiome Species 0.000 claims description 12
- 241000589516 Pseudomonas Species 0.000 claims description 7
- 238000012258 culturing Methods 0.000 claims description 5
- 241000722910 Burkholderia mallei Species 0.000 claims description 4
- 241001523681 Dendrobium Species 0.000 claims description 4
- 239000004480 active ingredient Substances 0.000 claims description 4
- 241001517197 Cattleya Species 0.000 claims description 2
- 241000519406 Paphiopedilum Species 0.000 claims description 2
- 241001505935 Phalaenopsis Species 0.000 claims description 2
- 241000732800 Cymbidium Species 0.000 claims 1
- 239000002609 medium Substances 0.000 description 8
- 230000012010 growth Effects 0.000 description 7
- 241000196324 Embryophyta Species 0.000 description 4
- 229920001817 Agar Polymers 0.000 description 3
- 239000000654 additive Substances 0.000 description 3
- 230000000996 additive effect Effects 0.000 description 3
- 239000008272 agar Substances 0.000 description 3
- 239000002207 metabolite Substances 0.000 description 3
- 239000006870 ms-medium Substances 0.000 description 3
- 239000006228 supernatant Substances 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- SIKJAQJRHWYJAI-UHFFFAOYSA-N Indole Chemical compound C1=CC=C2NC=CC2=C1 SIKJAQJRHWYJAI-UHFFFAOYSA-N 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000001965 increasing effect Effects 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 239000004475 Arginine Substances 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 241000588914 Enterobacter Species 0.000 description 1
- 241000588697 Enterobacter cloacae Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 239000004472 Lysine Substances 0.000 description 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
- 229910002651 NO3 Inorganic materials 0.000 description 1
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 102000004316 Oxidoreductases Human genes 0.000 description 1
- 108090000854 Oxidoreductases Proteins 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000000721 bacterilogical effect Effects 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Substances OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 1
- 238000012364 cultivation method Methods 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000001747 exhibiting effect Effects 0.000 description 1
- 229910000037 hydrogen sulfide Inorganic materials 0.000 description 1
- PZOUSPYUWWUPPK-UHFFFAOYSA-N indole Natural products CC1=CC=CC2=C1C=CN2 PZOUSPYUWWUPPK-UHFFFAOYSA-N 0.000 description 1
- RKJUIXBNRJVNHR-UHFFFAOYSA-N indolenine Natural products C1=CC=C2CC=NC2=C1 RKJUIXBNRJVNHR-UHFFFAOYSA-N 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 229960003104 ornithine Drugs 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 230000007226 seed germination Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 239000012137 tryptone Substances 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Description
【０００１】[0001]
【発明の属する技術分野】本発明は、ランの栽培促進剤
に関する。更に詳しくは、ランに適用されたとき成長促
進作用、苗条増加作用および発根促進などを示す栽培促
進剤に関する。TECHNICAL FIELD The present invention relates to an orchid cultivation promoter. More specifically, it relates to a cultivation promoting agent which exhibits a growth promoting action, a shoot increasing action and a rooting promoting action when applied to orchids .
【０００２】[0002]
【従来の技術】アルカリジェニス属に属する微生物の代
謝産物あるいはエンターバクター属に属する微生物の代
謝産物の凍結乾燥物を有効成分とする植物栽培促進剤
が、先に本出願人によって提案されている(特開平2-29
5,908号公報および同2-240,008号公報)。2. Description of the Related Art A plant cultivation promoter containing, as an active ingredient, a lyophilized product of a metabolite of a microorganism belonging to the genus Alcaligenis or a metabolite of a microorganism belonging to the genus Enterobacter has been previously proposed by the present applicant ( JP-A-2-29
5,908 and 2-240,008).
【０００３】これらの微生物の代謝産物による植物栽培
促進効果は、主として野菜や稲に向けられている。ま
た、エンターバクター・クロアカが植物の成長促進作用
を有することも知られているが、その対象は主として農
作物である(特開昭63-102,668号公報)。The plant cultivation promoting effect of the metabolites of these microorganisms is mainly directed to vegetables and rice. It is also known that Enterobacter cloacae has a plant growth promoting action, but its target is mainly agricultural products (Japanese Patent Laid-Open No. 63-102,668).
【０００４】[0004]
【発明が解決しようとする課題】本発明の目的は、微生
物を培養して得られた培養液を有効成分とし、ランの成
長促進作用、苗条増加作用および発根促進などを示す栽
培促進剤を提供することにある。An object of the present invention is to provide a culture promoting agent having a culture solution obtained by culturing a microorganism as an active ingredient and exhibiting orchid growth promoting action, shoot increasing action and rooting promoting action. To provide.
【０００５】[0005]
【課題を解決するための手段】かかる本発明の目的は、
シュードモナス属に属し、ランの栽培促進作用を有する
微生物Pseudomonas mallei No.206-1(FERM P-11898)を
培養して得られた培養液を有効成分とするランの栽培促
進剤によって達成される。The object of the present invention is as follows.
This is achieved by an orchid cultivation promoter containing a culture solution obtained by culturing a microorganism Pseudomonas mallei No. 206-1 (FERM P-11898) belonging to the genus Pseudomonas and having an orchid cultivation promoting action as an active ingredient.
【０００６】[0006]
【発明の実施の形態】ランの栽培促進作用を有するシュ
ードモナス属に属する微生物Pseudomonas mallei No.20
6-1(FERM P-11898)は、それを含む土壌、温泉水等の1g
(または1ml)をL-ブイヨン培地(トリプトン1%、酵母エキ
ス0.5%、NaCl 0.5%；殺菌前pH7.2)に添加し、37〜50℃
で24時間振とう培養することにより分離され、これがシ
ュードモナス属に属する微生物であることは、それの示
す後記の如き菌学的性質により、Bergey's Mannual of
Determinative Bacteriology 第9版から検索された。BEST MODE FOR CARRYING OUT THE INVENTION Pseudomonas mallei No. 20 , a microorganism belonging to the genus Pseudomonas having an orchid cultivation promoting action
6-1 (FERM P-11898) is 1g of soil, hot spring water, etc. containing it.
(Or 1 ml) was added to L-broth medium (tryptone 1%, yeast extract 0.5%, NaCl 0.5%; pH 7.2 before sterilization), and 37-50 ° C.
In separated by 24 hours shaking culture, which it is a microorganism belonging to the genus Pseudomonas, the later such mycological characteristics of showing of it, Bergey's Mannual of
Retrieved from Determinative Bacteriology 9th Edition.
【０００７】このシュードモナス属に属する微生物の培
養は、任意の培地10mlを用い、37〜40℃で約72〜90時間
程度振とう条件下で行われる。この培養液に、1N塩酸50
0μlを加えて菌を死滅させ、遠心分離(10000rpm、4℃、
10分間)して集菌し、その上澄液を採取して、これが添
加剤(栽培促進剤)として、植物培地に約4〜10ml/Ｌ、一
般には約4ml/Ｌの割合で添加されて用いられる。このよ
うな添加剤を添加した培地あるいはこれに寒天を添加
し、121℃のオートクレーブ中で約15〜20分間処理して
固化させた培地などには、ランの茎頂部が植え付けら
れ、明条件(12時間、4000ルクス)下で約2〜3ヶ月間培養
することが行われる。また、種子発芽による栽培やわき
芽分割による栽培などの栽培方法をとることもできる。[0007] Culture of microorganisms belonging to the genus Pseudomonas, using any medium 10 ml, is carried out under conditions shaking about 72-90 hours at 37 to 40 ° C.. 1N hydrochloric acid 50
Add 0 μl to kill the bacteria and centrifuge (10000 rpm, 4 ° C,
(10 minutes) to collect the bacteria and collect the supernatant, which is added as an additive (cultivation promoter) to the plant medium at a rate of about 4-10 ml / L, generally about 4 ml / L. Used. A medium added with such an additive or agar added thereto and treated in an autoclave at 121 ° C. for about 15 to 20 minutes to be solidified, or the like, has the shoot apex of the orchid planted, and the light condition ( Culturing is performed under 12 hours and 4000 lux) for about 2 to 3 months. Further, it is possible to adopt a cultivation method such as cultivation by seed germination or splitting of side buds.
【０００８】Pseudomonas mallei No.206-1は、下記の
如き菌学的性質を有する。
[形態]
細胞の形 桿菌
細胞の大きさ(μm) 0.5×1
運動性 あり
胞子 なし
グラム染色 陰性
[培地における成育状態]
肉汁寒天平板培養 黄褐色
滑らか
[生理的性質]
硝酸塩の還元 ＋
VPテスト ＋
インドールの生成 −
硫化水素の生成 −
クエン酸の利用 −
色素の生産 −
ウレアーゼ ＋
オキシダーゼ ＋
リジンの分解 −
オルニチンの分解 −
アルギニンの分解 ＋
生育範囲
pH 6〜10
温度(℃) 30〜50
酸素に対する態度 好気
糖類からの酸生成
D-グルコース ＋
D-マンニット ＋
アドニット −
L-アラビノース ＋
イノシット −
ソルビット −
麦芽糖 ＋
白糖 − Pseudomonas mallei No.206-1 is
It has the following bacteriological properties. [Morphology] Cell morphology Bacillus Cell size (μm) 0.5 × 1 Motile, no spores Gram stain Negative [Growth in medium] Meat agar plate culture Yellow brown Smooth [Physical properties] Nitrate reduction ＋ VP test ＋ Indole production-Hydrogen sulfide production-Citric acid utilization-Dye production-Urease + oxidase + Lysine decomposition-Ornithine decomposition-Arginine decomposition + Growth range pH 6-10 Temperature (° C) 30-50 Attitude toward oxygen Acid production from aerobic sugars D-glucose + D-mannite + adonite-L-arabinose + inosit-sorbit-maltose + sucrose-
【０００９】[0009]
【発明の効果】ランの栽培促進作用を有する微生物Pseu
domonas mallei No.206-1(FERM P-11898)を培養して得
られた培養液は、カトレア、パフィオペディウム、シン
ビジウム、ファレノプシス、デンドロビウム等のランの
栽培促進剤として成長促進効果、苗条増加および発根促
進効果を示している。[Effect of the Invention] Pseu , a microorganism having an orchid cultivation promoting action
The culture solution obtained by culturing domonas mallei No. 206-1 (FERM P-11898) was cattleya, paphiopedium, syn
As a cultivation promoter for orchids such as bidium, phalaenopsis, and dendrobium, they have a growth promoting effect, an increase in shoots and a root promoting effect.
【００１０】[0010]
【実施例】次に、実施例について本発明を説明する。EXAMPLES The present invention will now be described with reference to examples.
【００１１】実施例１
前記表に示される菌学的性質を示す微生物Pseudomonas
mallei No.206-1(FERMP-11898)が、前記記載の分離方法
により、長野県小谷村の温泉水より分離された(培養温
度40℃)。 Example 1 Pseudomonas microorganisms having the mycological properties shown in the above table
mallei No. 206-1 (FERMP-11898) was separated from the hot spring water in Otari Village, Nagano Prefecture by the separation method described above (culture temperature 40 ° C).
【００１２】これの培養は、L-ブイヨン培地を用いて、
35℃で72時間振とう条件下(120rpm)で行われ、前記記載
の方法で培養液からその上澄液を採取した。この上澄液
添加剤をMS培地に4ml/Ｌの割合で添加し、これに寒天を
8g/Ｌの割合で添加した後、121℃でのオートクレーブ処
理を15分間行い、培地を固化させた。この植物培地に、
ラン（パフィオペデュム）の茎頂部が植え付けられ、28
℃、4000ルクス、12時間の明条件下での栽培が2ヶ月間
行われた。 The culture of this was carried out using L-broth medium.
Performed under shaking conditions (120 rpm) for 72 hours at 35 ° C, as described above
The supernatant was collected from the culture broth by the method. This supernatant
Add the additive to MS medium at a rate of 4 ml / L and add agar to it.
After adding 8g / L, autoclave at 121 ℃
The treatment was performed for 15 minutes to solidify the medium. In this plant medium,
Orchid (Paphiopedum) shoots planted, 28
Cultivation under light conditions of 4,000 lux for 12 hours at 4000 ℃ for 2 months
It was conducted.
【００１３】その結果、MS培地そのものにラン（パフィ
オペデュム）の茎頂部を植え付けたものと比較して、2.
3倍の成長促進効果(茎葉成長の生育度で比較)がみられ
た。 As a result, the MS medium itself was run (puffy
Opedum), compared to the planted stem apex 2.
3-fold growth promotion effect (compared with the degree of foliage growth)
It was
【００１４】実施例２
前記表に示される菌学的性質を示す微生物Pseudomonas
mallei No.206-1 (FERM P-11898) の培養およびその培
養液を用いてのラン（デンドロビウム）の栽培が、実施
例１と同様に行われた。その結果、MS培地そのものにラ
ン（デンドロビウム）の茎頂部を植え付けたものと比較
して、4.6倍の発根促進効果がみられた。 Example 2 Pseudomonas microorganisms having the mycological properties shown in the above table
Cultivation of mallei No. 206-1 (FERM P-11898) and cultivation of orchid (dendrobium) using the culture solution were carried out in the same manner as in Example 1. La on the results, MS medium itself
As compared to those planted the shoot apex part of emissions (Dendrobium), 4.6 times the root promoting effect was observed.
フロントページの続き (58)調査した分野(Int.Cl.7，ＤＢ名) A01N 63/02 A01G 1/00 A01G 7/00 Front page continuation (58) Fields surveyed (Int.Cl. 7 , DB name) A01N 63/02 A01G 1/00 A01G 7/00
Claims (2)
進作用を有する微生物Pseudomonas mallei No.206-1(FE
RM P-11898)を培養して得られた培養液を有効成分とす
るランの栽培促進剤。1. A microorganism belonging to the genus Pseudomonas and having an action of promoting orchid cultivation Pseudomonas mallei No. 206-1 (FE
RM P-11898) An orchid cultivation promoter containing a culture solution obtained by culturing the same as an active ingredient.
シンビジウム、ファレノプシスまたはデンドロビウムで
ある請求項１記載のランの栽培促進剤。 2. An orchid is Cattleya, Paphiopedium,
With Cymbidium, Phalaenopsis or Dendrobium
The orchid cultivation accelerator according to claim 1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP31610795A JP3473228B2 (en) | 1995-11-09 | 1995-11-09 | Orchid cultivation promoter |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP31610795A JP3473228B2 (en) | 1995-11-09 | 1995-11-09 | Orchid cultivation promoter |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2002371389A Division JP3744894B2 (en) | 2002-12-24 | 2002-12-24 | Flower bud cultivation promoter |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH09132509A JPH09132509A (en) | 1997-05-20 |
| JP3473228B2 true JP3473228B2 (en) | 2003-12-02 |
Family
ID=18073330
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP31610795A Expired - Fee Related JP3473228B2 (en) | 1995-11-09 | 1995-11-09 | Orchid cultivation promoter |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JP3473228B2 (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2001302427A (en) * | 2000-04-25 | 2001-10-31 | Nok Corp | Plant growth accelerator and method for accelerating growth of plant by using the accelerator |
| JP4295806B1 (en) * | 2008-04-02 | 2009-07-15 | 理 松平 | Plant growth promoter |
| WO2018047918A1 (en) * | 2016-09-08 | 2018-03-15 | イビデン株式会社 | Plant activator and process for producing plant activator |
| CN107188734A (en) * | 2017-07-25 | 2017-09-22 | 兴义市龙安园林绿化有限公司 | A kind of Bougainvillea spectabilis Nutrition Soil and preparation method thereof |
-
1995
- 1995-11-09 JP JP31610795A patent/JP3473228B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| JPH09132509A (en) | 1997-05-20 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| LAPS | Cancellation because of no payment of annual fees |