JP5113965B2 - Inhibitors or inhibitors of the growth of heat- and acidophilic alicyclobacillus - Google Patents

Description

[0001]
BACKGROUND OF THE INVENTION
The present invention relates to a method for inhibiting or inhibiting the growth of 1,5-D-anhydrofructose heat-treated product of Alicyclobacillus spp. And an agent containing the same.
[0002]
[Prior art]
1,5-D-anhydrofructose can be produced from starch or starch degradation products by the action of α-1,4-glucan lyase, an enzyme present in plant tissues such as basidiomycetes or red alga. . 1,5-D-anhydrofructose has an interesting and unique structure in which glucose is dehydrated, and its functionality has been reported to have an inhibitory or inhibitory effect on bacterial growth.
[0003]
The sterilization standards for the production of soft drinks are stipulated by the Food Sanitation Law. For highly acidic beverages with a pH of less than 4.0, heat at 65 ° C for 10 minutes or more, and for acidic beverages with a pH of 4.0 to less than 4.6 Heated at 85 ° C. for 30 minutes or equivalent or higher. Conventionally, it was thought that sufficient sterilization effect was obtained under these sterilization conditions. However, in recent years, heat-resistant and acidophilic bacteria that survive and proliferate under these conditions have been discovered, and it is now a major problem in microbial management of soft drinks. The main bacterium to be managed is alicyclobacillus.
[0004]
[Problems to be solved by the invention]
An object of the present invention, soft drinks, in particular, highly acidic beverage containing fruit juice, against a strain of belonging to the genus Alicyclobacillus, which is one of the main causative bacteria of spoilage by microorganisms in acidic beverages, 1, 5-D --out based on the fact novel investigation of expressing a heat-treated product has excellent growth inhibitory or blocking effect of anhydrofructose, 1, 5-D- anhydro heat treatment product of the fructose belonging to the genus Alicyclobacillus strains of It is to provide a method for inhibiting or inhibiting proliferation.
[0005]
Another object of the present invention is to provide a 1, 5-D-anhydrofructose heat treatment was belonging to the genus Alicyclobacillus antiproliferative or inhibitor strains to the active ingredients.
Another object of the present invention is to provide use of a heat-treated product of 1,5-D-anhydrofructose for inhibiting or inhibiting the growth of a strain of the genus Alicyclobacillus.
Still other objects and advantages of the present invention will become apparent from the following description.
[0006]
[Means for Solving the Problems]
According to the present invention, the above objects and advantages of the present invention are as follows. First, 50 of 1,5-D-anhydrofructose, which exhibits higher antibacterial activity than that of 1,5-D-anhydrofructose. It is achieved by an agent for inhibiting or inhibiting the growth of an alicyclobacillus genus strain characterized by containing a heat-treated product at ˜150 ° C.
According to the present invention, the above-mentioned objects and advantages of the present invention are secondly achieved by a method for inhibiting or inhibiting the growth of an alicyclobacillus strain, characterized in that the above-mentioned agent of the present invention is contained in a soft drink. Is done.
In addition, according to the present invention, the above-mentioned objects and advantages of the present invention are, thirdly, 1,5-D-anhydrofructose exhibiting an antibacterial activity higher than that of 1,5-D-anhydrofructose. This is achieved by the use of the heat-treated product at 50 to 150 ° C. for inhibiting or inhibiting the growth of the strain of the genus Alicyclobacillus.
[0007]
In the present invention, 1,5-D-anhydrofructose is a heat-treated one. 1,5-D-anhydrofructose is a highly safe antibacterial substance, but its antibacterial activity is further enhanced by heat treatment. The heat treatment temperature is 50 to 150 ° C., and the heat treatment time is preferably 1 second to 100 hours. For example, treatment at 50 ° C. under normal pressure for 10 minutes to 100 hours, treatment at 95 ° C. for 1 minute to 10 hours, high pressure at 120 ° C. for 10 seconds to 2 hours, 130 to 150 ° C. for 1 second to 30 minutes, etc. It is possible to select the heat treatment conditions.
[0008]
When the present invention is applied to a soft drink, 1,5-D-anhydrofructose, which is higher in antibacterial activity than 1,5-D-anhydrofructose , has been heated at 50 to 150 ° C. The content is not particularly limited, but it is preferably 0.01 to 10 parts by weight per 100 parts by weight of the soft drink from the viewpoint of the expression of antibacterial activity against the strain of the genus Alicyclobacillus and the influence on the taste of the soft drink. More preferably, it is 0.1 to 5 parts by weight.
[0009]
When added to a soft drink, subsequent heating of the drink for sterilization or the like will not have any adverse effect.
Although heating conditions change with the soft drinks, it can heat, adding the heat-processed material of 1 , 5-D-anhydrofructose , and can also heat after adding. Appropriate heating conditions are 10 to 30 minutes at 60 ° C. or about 2 minutes at 90 ° C. for beverages having a pH of 4.0 or less, and 85 to 30 minutes for pH 4.0 to 4.6 beverages, pH 4 For beverages of 6 or more, 125 ° C for 5 to 30 minutes, or 130 to 150 ° C for about 1 to 2 seconds.
[0010]
In addition to 1,5-D-anhydrofructose, the agent of the present invention can contain other inert carriers and adjuvants. Examples of inert carriers include starch, maltodextrin, cyclodextrin, roasted dextrin, sucrose, glucose, maltose, lactose and other sugars, carboxymethylcellulose, agar, agar degradation products, carrageenan, glucomannan, locust bean gum, Thickening polysaccharides such as xanthan gum, grain flours such as wheat flour, rice flour and corn flour, proteins such as defatted soybeans, defatted milk powder, and corn protein, and in the case of liquid or gel form, in addition to the above substances, water, alcohol Examples thereof include substances that are liquid at room temperature and normal pressure.
[0011]
Examples of the adjuvant include adipic acid, propionic acid, sodium propionate, calcium propionate, lactic acid, sodium lactate, calcium lactate, citric acid, trisodium citrate, sorbic acid, potassium sorbate, succinic acid, and succinic acid. Sodium, disodium succinate, fumaric acid, monosodium fumarate, gluconic acid, sodium gluconate, calcium gluconate, DL-tartaric acid, L-tartaric acid, DL-sodium tartrate, DL-malic acid, DL-sodium malate, Benzoic acid, sodium benzoate, glucono delta lactone, carbonates, carbon dioxide, nitrite, phosphoric acid, phosphates, polymerized phosphates (sodium pyrophosphate, sodium tripolyphosphate, hexametaphosphate, etc.), itaconic acid, Various acids such as phytic acid and Mention may be made of a kind.
The agent of the present invention can be various dosage forms such as solutions, granules, powders, tablets, suspensions, gels and the like.
[0012]
Moreover, the agent of this invention can contain other various antioxidant substances other than the heat-processed material of 1 , 5-D-anhydrofructose. For example, ascorbic acid, its sodium salt, potassium salt, calcium salt, fatty acid ester, erythorbic acid, its sodium salt, potassium salt, calcium salt, fatty acid ester, α-tocopherol, β-tocopherol, γ-tocopherol, δ-tocopherol, β-carotene, carotenoids, catechins, tannins, flavonoids, anthocyanins, polyphenols, BHT, BHA, uric acid, DHA, IPA, EPA, EDTA, guaiac fat, isopropyl citrate, nordihydroguaiaretic acid, propyl gallate, etc. Can be mentioned.
[0013]
Moreover, the agent of this invention can also contain another antibacterial substance other than the heat-processed material of 1 , 5-D-anhydrofructose. Antibacterial substances include, for example, acetic acid, sodium acetate, glycerin, fatty acid ester, polyglycerin fatty acid ester, sugar ester, thiamine lauryl sulfate, sodium dehydroacetate, glycine, protamine, polylysine, egg white lysozyme, chitosan, ethanol, wasabi extract , Mustard extract, globe extract, cinnamon extract, sage extract, pimenta extract, pepper extract, rosemary extract, oregano extract, garlic extract, fig leaf extract, citrus seed extract, mulberry extract , Succinic acid, perilla extract, ginger extract, tade extract, hop extract, raw soybean extract, grape skin extract, hokkoshi extract, moso bamboo extract, pear extract, propolis extract, licorice oil extract , Olive extract, yucca foam extract, red yeast rice Hydrolyzate, pectin decomposition products, tea tannin, hinokitiol, caffeic acid, cinnamic acid, p- coumaric acid, ferulic acid, and cinnamic acid homologues such as chlorogenic acid.
[0014]
【Example】
Hereinafter, the present invention will be described in more detail by way of examples. The present invention is not limited to the embodiment. Incidentally, 1, 5-D- anhydroerythromycin heat treatment product of fructose in the examples, 1, 5-D-anhydrofructose (purity 40.5%, Bx71.2) was 15 minutes heat treatment at 120 ° C. Is.
[0015]
Example 1 and Comparative Example 1
Concentrated reduced apple juice (Bx12) to which 1,5-D-anhydrofructose heat-treated product was added at 0.5 wt%, 1 wt% or 3 wt% was added with 10 aliquots of previously cultured Alicyclobacillus acidoterrestris. It added so that it might become ⁵ pieces / ml. In addition, as comparative examples, juices without addition of 1,5-D-anhydrofructose were prepared in the same manner, and these juices were stored at 40 ° C. and evaluated by measuring the number of bacteria over time. The results are shown in FIG.
[0016]
Example 2 and Comparative Example 2
Concentrated reduced apple juice (Bx12) to which 3% by weight of 1,5-D-anhydrofructose heat-treated product was added, and previously cultivated Alicyclobacillus acidoterrestris was added to 10 ⁶ / ml did. As comparative examples, juices without addition of 1,5-D-anhydrofructose were prepared in the same manner, and these juices were heated at 95 ° C. and the number of bacteria was measured over time. The results are shown in FIG.
【Effect of the invention】
According to the present invention, by using a heat-treated product of 1,5-D-anhydrofructose , it is possible to suppress or prevent the growth of an alicyclobacillus strain in foods and drinks.
[Brief description of the drawings]
[1] be adversely the growth of Alicyclobacillus Bacillus subtilis in the concentrate apple juice, 1, the amount of the influence of 5-D-anhydrofructose heat treatment product of the fructose.
[Figure 2] to adversely the growth of Alicyclobacillus Bacillus strain in the concentrate apple juice, 1, of 5-D-anhydrofructose heating time after addition of the heat treatment product of the impact.
[Explanation of symbols]
(A) No heat-treated product of 1,5-D-anhydrofructose added.
(B) 1,5-D-anhydrofructose heat-treated product 0.5% by weight added.
(C) Addition of 1% by weight of a heat-treated product of 1,5-D-anhydrofructose.
(D) Addition of 3% by weight of a heat-treated product of 1,5-D-anhydrofructose.

Claims (5)

Aricyclo is characterized by containing a heat-treated product of 1,5-D-anhydrofructose at 50 to 150 ° C., which exhibits antibacterial activity higher than that of 1,5-D-anhydrofructose. Growth inhibitor or inhibitor for Bacillus strains. The agent of Claim 1 whose time of heat processing is 1 second-100 hours.   A method for inhibiting or inhibiting the growth of a strain of the genus Alicyclobacillus, which comprises adding the agent according to claim 1 or 2 to a soft drink.   A method for inhibiting or inhibiting the growth of an alicyclobacillus strain, wherein the agent according to claim 1 or 2 is heated while being added to a soft drink or is added to a soft drink and then heated. Inhibition of growth of alicyclobacillus strains by heat treatment of 1,5-D-anhydrofructose at 50 to 150 ° C., which exhibits higher antibacterial activity than that of 1,5-D-anhydrofructose Or use for prevention.

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