JP5871412B2 - ヒト化マウス - Google Patents

ヒト化マウス Download PDF

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Publication number
JP5871412B2
JP5871412B2 JP2014507305A JP2014507305A JP5871412B2 JP 5871412 B2 JP5871412 B2 JP 5871412B2 JP 2014507305 A JP2014507305 A JP 2014507305A JP 2014507305 A JP2014507305 A JP 2014507305A JP 5871412 B2 JP5871412 B2 JP 5871412B2
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JP
Japan
Prior art keywords
mouse
gene
human
cells
derived
Prior art date
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Expired - Fee Related
Application number
JP2014507305A
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English (en)
Japanese (ja)
Other versions
JPWO2013145331A1 (ja
Inventor
研一 山村
研一 山村
喜美 荒木
喜美 荒木
誠治 岡田
誠治 岡田
明彦 下野
明彦 下野
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Transgenic Inc
Kumamoto University
Original Assignee
Transgenic Inc
Kumamoto University
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Priority to JP2014507305A priority Critical patent/JP5871412B2/ja
Publication of JPWO2013145331A1 publication Critical patent/JPWO2013145331A1/ja
Application granted granted Critical
Publication of JP5871412B2 publication Critical patent/JP5871412B2/ja
Expired - Fee Related legal-status Critical Current
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0271—Chimeric vertebrates, e.g. comprising exogenous cells
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A01K67/0278—Knock-in vertebrates, e.g. humanised vertebrates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0676—Pancreatic cells
    • C12N5/0678—Stem cells; Progenitor cells; Precursor cells
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2207/00—Modified animals
    • A01K2207/12—Animals modified by administration of exogenous cells
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2207/00—Modified animals
    • A01K2207/20—Animals treated with compounds which are neither proteins nor nucleic acids
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A01K2217/075—Animals genetically altered by homologous recombination inducing loss of function, i.e. knock out
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/105—Murine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/03—Animal model, e.g. for test or diseases
    • A01K2267/0331—Animal model for proliferative diseases
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/03—Animal model, e.g. for test or diseases
    • A01K2267/035—Animal model for multifactorial diseases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2517/00—Cells related to new breeds of animals
    • C12N2517/02—Cells from transgenic animals

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Environmental Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Developmental Biology & Embryology (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Reproductive Health (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Microbiology (AREA)
  • Veterinary Medicine (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2014507305A 2012-03-27 2012-03-27 ヒト化マウス Expired - Fee Related JP5871412B2 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2014507305A JP5871412B2 (ja) 2012-03-27 2012-03-27 ヒト化マウス

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
PCT/JP2012/058790 WO2013145331A1 (fr) 2012-03-27 2012-03-27 Souris humanisée
JP2014507305A JP5871412B2 (ja) 2012-03-27 2012-03-27 ヒト化マウス

Publications (2)

Publication Number Publication Date
JPWO2013145331A1 JPWO2013145331A1 (ja) 2015-12-10
JP5871412B2 true JP5871412B2 (ja) 2016-03-01

Family

ID=49258692

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2014507305A Expired - Fee Related JP5871412B2 (ja) 2012-03-27 2012-03-27 ヒト化マウス

Country Status (9)

Country Link
US (1) US20150052625A1 (fr)
EP (1) EP2832221A4 (fr)
JP (1) JP5871412B2 (fr)
KR (1) KR101708541B1 (fr)
CN (1) CN104378975A (fr)
AU (1) AU2012374750B2 (fr)
CA (1) CA2868292A1 (fr)
HK (1) HK1202773A1 (fr)
WO (1) WO2013145331A1 (fr)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107709550A (zh) * 2015-06-18 2018-02-16 转基因股份有限公司 脏器人源化小鼠
CN105145486A (zh) * 2015-07-22 2015-12-16 中国医学科学院医学实验动物研究所 Rag2基因敲除大鼠在建立个性化肿瘤治疗模型中的应用
WO2018161417A1 (fr) * 2017-03-07 2018-09-13 浙江大学 Procédé de construction d'un modèle de souris humanisé pour l'hépatite b chronique par le biais de cellules souches
CN109735485B (zh) * 2019-01-29 2021-04-16 深圳市拓普生物科技有限公司 人源化肝脏动物模型及其构建方法和应用
EP3978607B1 (fr) 2019-05-27 2025-08-20 Transgenic Inc. Souris à exons humanisés
JP7039045B2 (ja) 2019-08-26 2022-03-22 株式会社フェニックスバイオ ヒト脂肪肝モデル細胞
CA3211975A1 (fr) 2021-02-26 2022-09-01 Phoenixbio Co., Ltd. Cellules de modele de foie gras humain a utiliser dans un procede de criblage
CN116530470B (zh) * 2022-12-26 2025-09-23 华中科技大学同济医学院附属同济医院 一种自身免疫性肝炎小鼠模型的创建方法及应用
CN117882676B (zh) * 2024-02-09 2025-09-26 中国人民解放军总医院第八医学中心 一种谱系示踪小鼠模型及其构建方法和应用

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE220421T1 (de) * 1990-02-26 2002-07-15 Univ Leland Stanford Junior Identifizierung und expression von insekten- steroidrezeptor-dns-sequenzen
EP0910652B1 (fr) * 1996-04-05 2014-10-08 The Salk Institute For Biological Studies Techniques liees a une utilisation d'hormones visant a moduler l'expression de genes exogenes chez des mammiferes et produits connexes
JP4664554B2 (ja) 1999-07-14 2011-04-06 株式会社トランスジェニック トラップベクター及びこれを用いた遺伝子トラップ法
US6509514B1 (en) * 2000-03-17 2003-01-21 Kmt Hepatech, Inc. Chimeric animal model susceptible to human hepatitis C virus infection
JP4836503B2 (ja) 2005-06-27 2011-12-14 株式会社トランスジェニック ノックアウト非ヒト動物
WO2007113505A2 (fr) * 2006-03-30 2007-10-11 The University Court Of The University Of Edinburgh Milieu de culture contenant des inhibiteurs de kinase et utilisation de celui-ci
CN102281758B (zh) * 2009-01-16 2014-12-10 公益财团法人实验动物中央研究所 移植了人肝细胞的小鼠
WO2011068103A1 (fr) 2009-12-01 2011-06-09 独立行政法人国立がん研究センター Procédé de construction d'un rat chimérique à l'aide de cellules souches embryonnaires de rat
JPWO2011149102A1 (ja) * 2010-05-24 2013-07-25 国立大学法人 熊本大学 生体イメージングに最適化された高度免疫不全マウス

Also Published As

Publication number Publication date
EP2832221A4 (fr) 2015-10-14
AU2012374750B2 (en) 2017-02-02
JPWO2013145331A1 (ja) 2015-12-10
CN104378975A (zh) 2015-02-25
HK1202773A1 (en) 2015-10-09
AU2012374750A1 (en) 2014-10-16
EP2832221A1 (fr) 2015-02-04
US20150052625A1 (en) 2015-02-19
KR101708541B1 (ko) 2017-02-20
WO2013145331A1 (fr) 2013-10-03
CA2868292A1 (fr) 2013-10-03
KR20140144698A (ko) 2014-12-19

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