JP7728242B2 - 抗体分析のための親和性クロマトグラフィー結合ネイティブ質量分析 - Google Patents

抗体分析のための親和性クロマトグラフィー結合ネイティブ質量分析

Info

Publication number
JP7728242B2
JP7728242B2 JP2022504073A JP2022504073A JP7728242B2 JP 7728242 B2 JP7728242 B2 JP 7728242B2 JP 2022504073 A JP2022504073 A JP 2022504073A JP 2022504073 A JP2022504073 A JP 2022504073A JP 7728242 B2 JP7728242 B2 JP 7728242B2
Authority
JP
Japan
Prior art keywords
protein
antibody
peptide
mass spectrometer
fcγriiia
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2022504073A
Other languages
English (en)
Japanese (ja)
Other versions
JP2022541920A (ja
JP2022541920A5 (fr
Inventor
シュンハイ ワン
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Regeneron Pharmaceuticals Inc
Original Assignee
Regeneron Pharmaceuticals Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Regeneron Pharmaceuticals Inc filed Critical Regeneron Pharmaceuticals Inc
Publication of JP2022541920A publication Critical patent/JP2022541920A/ja
Publication of JP2022541920A5 publication Critical patent/JP2022541920A5/ja
Priority to JP2025120146A priority Critical patent/JP2025157406A/ja
Application granted granted Critical
Publication of JP7728242B2 publication Critical patent/JP7728242B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/62—Detectors specially adapted therefor
    • G01N30/72—Mass spectrometers
    • G01N30/7233—Mass spectrometers interfaced to liquid or supercritical fluid chromatograph
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854—Immunoglobulins
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J49/00—Particle spectrometers or separator tubes
    • H01J49/02—Details
    • H01J49/10—Ion sources; Ion guns
    • H01J49/16—Ion sources; Ion guns using surface ionisation, e.g. field-, thermionic- or photo-emission
    • H01J49/165—Electrospray ionisation
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J49/00—Particle spectrometers or separator tubes
    • H01J49/26—Mass spectrometers or separator tubes
    • H01J49/34—Dynamic spectrometers
    • H01J49/42—Stability-of-path spectrometers, e.g. monopole, quadrupole, multipole, farvitrons
    • H01J49/4205—Device types
    • H01J49/422—Two-dimensional RF ion traps
    • H01J49/4225—Multipole linear ion traps, e.g. quadrupoles, hexapoles
    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01J—ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
    • H01J49/00—Particle spectrometers or separator tubes
    • H01J49/26—Mass spectrometers or separator tubes
    • H01J49/34—Dynamic spectrometers
    • H01J49/42—Stability-of-path spectrometers, e.g. monopole, quadrupole, multipole, farvitrons
    • H01J49/4205—Device types
    • H01J49/4245—Electrostatic ion traps
    • H01J49/425—Electrostatic ion traps with a logarithmic radial electric potential, e.g. orbitraps
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2440/00—Post-translational modifications [PTMs] in chemical analysis of biological material
    • G01N2440/38—Post-translational modifications [PTMs] in chemical analysis of biological material addition of carbohydrates, e.g. glycosylation, glycation

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Biophysics (AREA)
  • Plasma & Fusion (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Peptides Or Proteins (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2022504073A 2019-07-22 2020-07-22 抗体分析のための親和性クロマトグラフィー結合ネイティブ質量分析 Active JP7728242B2 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2025120146A JP2025157406A (ja) 2019-07-22 2025-07-17 抗体分析のための親和性クロマトグラフィー結合ネイティブ質量分析

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201962877037P 2019-07-22 2019-07-22
US62/877,037 2019-07-22
US201962907485P 2019-09-27 2019-09-27
US62/907,485 2019-09-27
PCT/US2020/043098 WO2021016366A1 (fr) 2019-07-22 2020-07-22 Spectrométrie de masse native couplée à une chromatographie d'affinité pour analyse d'anticorps

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2025120146A Division JP2025157406A (ja) 2019-07-22 2025-07-17 抗体分析のための親和性クロマトグラフィー結合ネイティブ質量分析

Publications (3)

Publication Number Publication Date
JP2022541920A JP2022541920A (ja) 2022-09-28
JP2022541920A5 JP2022541920A5 (fr) 2023-06-07
JP7728242B2 true JP7728242B2 (ja) 2025-08-22

Family

ID=72050922

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2022504073A Active JP7728242B2 (ja) 2019-07-22 2020-07-22 抗体分析のための親和性クロマトグラフィー結合ネイティブ質量分析
JP2025120146A Pending JP2025157406A (ja) 2019-07-22 2025-07-17 抗体分析のための親和性クロマトグラフィー結合ネイティブ質量分析

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2025120146A Pending JP2025157406A (ja) 2019-07-22 2025-07-17 抗体分析のための親和性クロマトグラフィー結合ネイティブ質量分析

Country Status (11)

Country Link
US (1) US12535490B2 (fr)
EP (1) EP4004557A1 (fr)
JP (2) JP7728242B2 (fr)
KR (1) KR102889153B1 (fr)
CN (1) CN114430804A (fr)
AU (1) AU2020315834B2 (fr)
BR (1) BR112022000780A2 (fr)
CA (1) CA3147426A1 (fr)
IL (1) IL289804A (fr)
MX (1) MX2022000836A (fr)
WO (1) WO2021016366A1 (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115656350B (zh) * 2022-09-05 2024-11-12 华熙生物科技股份有限公司 一种确定交联透明质酸的修饰位点和交联方式的方法
CN115684390B (zh) * 2022-09-30 2025-04-22 辽宁键凯科技有限公司 一种抗体药物偶联物的偶联效果的检测方法

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2014531596A (ja) 2011-09-29 2014-11-27 シアトル ジェネティクス，インコーポレイティド タンパク質が結合した作用物質化合物のインタクト質量の測定
JP2016502085A (ja) 2013-01-15 2016-01-21 リブゾン マブファーム インコーポレイティド タンパク質の精製プロセスにおける試料のグリコシル化および末端修飾状況を測定する方法
JP2018501211A (ja) 2014-11-27 2018-01-18 ザイムワークス，インコーポレイテッド Ｈｅｒ２を標的とする二重特異性抗原結合性構築物の使用方法
US20190194298A1 (en) 2017-12-22 2019-06-27 Regeneron Pharmaceuticals, Inc. System and method for characterizing drug product impurities

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112014024023A2 (pt) 2012-03-28 2017-07-18 Genentech Inc anticorpos idiotípicos anti-hcmv e usos dos mesmos
EA202192077A1 (ru) 2019-01-25 2021-10-05 Ридженерон Фармасьютикалз, Инк. Хроматография на основе белка а - масс-спектрометр с ионизацией электрораспылением

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2014531596A (ja) 2011-09-29 2014-11-27 シアトル ジェネティクス，インコーポレイティド タンパク質が結合した作用物質化合物のインタクト質量の測定
JP2016502085A (ja) 2013-01-15 2016-01-21 リブゾン マブファーム インコーポレイティド タンパク質の精製プロセスにおける試料のグリコシル化および末端修飾状況を測定する方法
JP2018501211A (ja) 2014-11-27 2018-01-18 ザイムワークス，インコーポレイテッド Ｈｅｒ２を標的とする二重特異性抗原結合性構築物の使用方法
US20190194298A1 (en) 2017-12-22 2019-06-27 Regeneron Pharmaceuticals, Inc. System and method for characterizing drug product impurities

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
Rabah Gahoual et al，，Detailed Characterization of Monoclonal Antibody Receptor Interaction Using Affinity Liquid Chromatography Hyphenated to Native Mass Spectrometry，Analytical Chemistry，2017年04月11日，89(10)，5404-5412
S Canarelli et al，On-line microdialysis of proteins with high-salt buffers for direct coupling of electrospray ionization mass spectrometry and liquid chromatography，Journal of Chromatography A，2002年，948，139-149
Thomas MOHRIN，オービトラップ型イオントラップを有する質量分析計の原理と応用研究，ぶんせき / 日本分析化学会 編 2018 (12)，2018年12月，526-531

Also Published As

Publication number Publication date
AU2020315834B2 (en) 2025-12-18
JP2022541920A (ja) 2022-09-28
US12535490B2 (en) 2026-01-27
CN114430804A (zh) 2022-05-03
WO2021016366A1 (fr) 2021-01-28
KR102889153B1 (ko) 2025-11-24
MX2022000836A (es) 2022-08-04
JP2025157406A (ja) 2025-10-15
CA3147426A1 (fr) 2021-01-28
KR20220035443A (ko) 2022-03-22
BR112022000780A2 (pt) 2022-07-19
EP4004557A1 (fr) 2022-06-01
AU2020315834A1 (en) 2022-02-03
US20210025899A1 (en) 2021-01-28
IL289804A (en) 2022-03-01

Similar Documents

Publication Publication Date Title
JP2025157406A (ja) 抗体分析のための親和性クロマトグラフィー結合ネイティブ質量分析
US11932684B2 (en) Online chromatography and electrospray ionization mass spectrometer
US11385239B2 (en) Method and system of identifying and quantifying a protein
JP2023052110A (ja) タンパク質を同定及び定量化する方法及びシステム
AU2020212040A1 (en) Protein a chromatography - electrospray ionization mass spectrometer
US12169204B2 (en) Coupled native mass spectrometry for antibody analysis
EA048152B1 (ru) Хроматография гидрофобного взаимодействия, сопряженная с нативной масс-спектрометрией, для анализа антител
HK40048586B (en) Method and system of identifying and quantifying a protein
HK40048586A (en) Method and system of identifying and quantifying a protein
EA042991B1 (ru) Способ и система идентификации и количественного определения белка

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20230530

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20230530

RD04 Notification of resignation of power of attorney

Free format text: JAPANESE INTERMEDIATE CODE: A7424

Effective date: 20230725

A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20240221

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20240304

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20240912

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20241212

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20241226

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20250318

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20250717

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20250804

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20250812

R150 Certificate of patent or registration of utility model

Ref document number: 7728242

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150