JPH01185443A - Instrument for measuring antibacterial activity - Google Patents

Abstract

PURPOSE:To treat large amount of objects to be tested by one person, by respectively inserting the lower protruding parts of filters in the small chambers having a culture solution provided in a container and subsequently reversing a support plate to turn the regions not immersed in the culture solution of said filter downwardly and placing the filters on the surface of an agar medium inoculated with test bacteria. CONSTITUTION:A handle 3 is grasped to allow a support plate 1 to face the upper surface of a container 6 and rod-shaped filters 2 are inserted in respective small chambers 5 standing opposite to each other and the lower parts of the filters 2 are continuously immersed in the culture solutions 4 received in the small chambers 5. An agar medium is spread in other container 7 and the upper surface thereof is inoculated with test bacteria. After the culture solutions 4 reach the upper ends of the filters 2, the support 1 of the filters 2 is reversed. Subsequently, the upper and lower positions of the filters are reversed to place the end parts of the filters 2 on the upper surface of the test bacteria allowed to inoculate the upper surface of the agar medium 8. As a result, it becomes unnecessary to successively prepare the sample of separated bacteria at every one and antibacterial activity can be measured extremely rapidly and easily.

Description

[Detailed Description of the Invention] [Industrial Application Field] This invention relates to an antibacterial activity measuring device, and in particular, an antibacterial activity measuring device for carrying out an antibacterial activity test of antibiotic-producing bacteria or antibiotics. It is related to.

[Conventional technology] In the conventional antibacterial activity test, isolated bacterial strains are inoculated on the surface of an agar medium in a straight line parallel to each other, and when they grow in a strip shape, several types of test bacteria are inoculated in a straight line parallel in a direction perpendicular to this. Inoculate and culture. In this case, since test bacteria do not grow near the colony of the isolated bacterial strain that secretes antibacterial substances, the length of this growth inhibition zone is measured to determine the antibacterial activity against each test bacteria. The isolated bacterial strain is cultured in liquid and grown to a diameter of 5 m.
It is soaked into a circular filter paper disk of size m, and the disk is placed on the surface of an agar medium inoculated with the test bacteria and cultured at an appropriate temperature. If the isolated strain produces antibiotics, the growth of the test bacteria is inhibited and a transparent circle called an inhibition circle is formed around the disk. The antibacterial potency was determined by measuring the diameter of this circle.

[Problem to be solved by the invention] As mentioned above, the conventional method has to be carried out individually by hand, so when processing a large number of test objects, it requires a large number of manpower and a large amount of time. There was a problem with this.

SUMMARY OF THE INVENTION In order to solve the above-mentioned problems, the present invention aims to provide an antibacterial activity measuring instrument that allows one person to process a large number of test subjects.

[Means for Solving the Problems] The present invention was proposed to achieve the above object, and includes forming a large number of small holes in a support plate in a predetermined arrangement, and inserting a filter into each of the small holes. The filter is inserted into the support plate so as to protrude from both sides of the support plate, while a container in which a small chamber for accommodating the culture solution of the isolated bacteria is provided at a location opposite to the small hole, and the filter is inserted into the support plate. One end of each filter inserted into each small hole is immersed in the corresponding small chamber's 1iII bacterial culture solution, and after the culture solution reaches the other end of the filter due to capillary action, the support The plate is inverted and the other end of the filter is placed on the surface of the agar medium inoculated with the test bacteria and cultured, thereby making it possible to measure the antibacterial activity of the isolated bacteria or antibiotic against the test bacteria. The purpose is to provide an antibacterial power measurement device that can

[Operation] In the present invention, the filter is fitted into small holes regularly arranged in a support plate, and both sides of the filter are provided to protrude from both sides of the support plate. Then, grasp the support plate and insert the lower protrusion of the filter into each corresponding compartment of the container. Since the culture fluids of various types of isolated bacteria are stored in these small chambers, the culture fluids rise within each filter due to capillary action and reach one end on the opposite side. Therefore, the support plate supporting the filter was inverted, and the part not immersed in the culture solution was placed on the agar medium surface inoculated with the test bacteria, with the part not immersed in the culture solution facing downward. If the container containing the microorganisms is covered and cultured, it is possible to assay as many samples as there are small holes in the support plate at the same time.

[Example 1] Hereinafter, an example of the present invention will be described in detail with reference to the accompanying drawings. In FIG. 1, 24 small holes (IaHla) are orderly drilled in a rectangular support plate (1).

Then, a rod-shaped filter (
2) (2)... is pushed through, and the rod-shaped filter (2)... is pushed through.
) are provided so as to protrude from both sides of the support plate (1). In the illustrated example, the vicinity of one end of the rod-shaped filter (2) is clamped, but the present invention is not limited to this. Furthermore, the number of said apertures (la) (la) should not be specified either. Further, the rod-shaped filter (2) has an outer shell (21) to maintain its rod shape, and the filter (2h) is fitted inside the outer shell (21).

U-shaped handles (3) are rotatably pivoted on both sides of the central portion of the support plate (1) in the longitudinal direction. Thus, the handle (3) can be pivoted to a slightly tightened degree so as to stop at any position. Therefore, the handle (3)
Of course, it is suitable for gripping, but it also has a size that allows the front and front screens of the support plate ( ) to be reversed.

On the other hand, a container (6) having small chambers (5), (5), etc. containing culture solutions (4), (4), etc. of isolated bacteria is prepared.

The small chambers (5) (5)... are the support plate (1).
The small holes (la) (la)... are provided facing each other.

Therefore, if there are 24 small holes (la), (la), . . ., the number of small chambers (5), (5), . . . can also be 24.

Therefore, for example, culture solutions of 24 types of isolated bacteria can be stored separately in each of the small chambers (5), (5), . . . .

Therefore, as shown in Figure 3, each of the small rooms (5), (5),
One end of the rod-shaped filter (2) (2) ... fitted into each small hole (IaHla) ... of the support plate in each culture solution (4) (4) ... housed in ... Soak the parts. That is, as shown in the figure, hold the handle (3) and press the support plate (1).
facing the upper surface of the container (6), and each small chamber (5) (5) facing each rod-shaped filter (2) (2), respectively.
...Insert inside. Then, the rod-shaped filter (2) (
2) The lower portions of the cells (5), (5), and so on are immersed in the respective culture solutions (4), (4), and so on contained in the small chambers (5), (5), and so on. In such a case, use a rod-shaped filter (2X2)...
The culture solution (4) (4)... is infiltrated into each of the rod-shaped filters (2) (2)... by capillary action. It reaches the upper end of .

Also, as shown in Figure 4, an agar medium (
8) is spread, and test bacteria are inoculated onto the top surface of the agar medium (8). Therefore, as described above (, culture solution (
4) After (4)... reaches the upper end of the rod-shaped filter (2) (2)..., the rod-shaped filter (2) (2)...
Flip over the support plate (+). Then, the rod-shaped filter (
2) Fill the upper and lower positions of (2)... and place the ends of each rod-shaped filter (2) (2)... on the top surface of the test bacteria inoculated on the top surface of the agar medium (8).

The handle (3) is attached to the lid (9) of the container (7).
Place the side entrance in a position where it will not interfere with the installation of the lid.
The lid (9) is placed on the lid and cultured for a predetermined period of time.

In this way, if the isolated bacteria infiltrating the rod-shaped filter (2) produce antibiotics, a transparent inhibition circle (not shown) is formed around the rod-shaped filter (2) to prevent the growth of the test bacteria. ) can be done. Therefore, by measuring the diameter of this circle, the antibacterial titer can be determined.

Thus, in this example, various samples of 24 isolated bacteria can be collected at the same time into a rod-shaped filter (2M2), and the collection method is simple, and the remaining state is Then, the rod-shaped filters (2), (2), etc. are inverted and placed on the top surface of the agar medium (8) for culturing, so samples of isolated bacteria for each are prepared one after another as in the conventional method. There is no need for this, and antibacterial activity measurements can be carried out extremely easily and quickly.

[Effects of the Invention] As will be described in detail in one embodiment of the present invention (a filter is held between each small hole provided in a support plate, and this filter protrudes from both upper and lower sides of the small hole). Insert one protrusion into each chamber of the container containing the culture solution of the isolated bacteria, and immerse the lower part of the filter in the culture solution.In this case, the culture solution will be absorbed by capillary action. to reach the upper end of the filter.In this way, the objects to be inspected do not have to be created individually, but can be created at the same time.Furthermore, by reversing the support plate supporting the filter, the upper end of the filter can be moved downward. Invert. Then, pre-invert each end of the inverted filter.
Place it on top of the test bacteria inoculated on the agar medium, and
A container containing the agar medium is covered and cultured. If antibiotics are made, the growth of test bacteria will be inhibited around the filter, creating a transparent inhibition circle. Therefore,
By measuring the diameter of this circle, the antibacterial titer can be determined.

As described above, this invention makes it possible to test a large number of samples at the same time, but various modifications can be made without departing from the spirit of the invention, and this invention can be modified without departing from the spirit of the invention. It goes without saying that this extends to many things.

[Brief explanation of the drawing]

The figures show one embodiment of the present invention, in which Fig. 1 is a front view of the support plate holding the filter, Fig. 2 is a plan view of the same, and Fig. 3 is a container containing a culture solution of isolated bacteria. FIG. 4 is a vertical cross-sectional view showing the filter placed on an agar medium inoculated with test bacteria. Code explanation 1: Support plate la: Small hole 2: Rod-shaped filter 4: Culture solution 5: Small chamber 6, 7: Container 8: Agar medium

Claims (1)

[Claims] A large number of small holes are drilled in a predetermined arrangement in the support plate, and a filter is inserted into each of the small holes so that the filter protrudes from both sides of the support plate, while a portion facing the small holes is formed. A container is provided with a small chamber for accommodating a culture solution of the isolated bacteria, and one end of each filter inserted into each small hole of the support plate is immersed in the culture solution of the isolated bacteria in the corresponding small chamber. After the culture solution reaches the other end of the filter due to capillary action, the support plate is inverted and the other end of the filter is placed on the surface of the agar medium inoculated with the test bacteria and cultured. . An antibacterial power measuring instrument, characterized in that it is capable of measuring the antibacterial power of the isolated bacteria or antibiotic against the test bacteria.