JPH01238532A - Antiinfectant - Google Patents

Abstract

PURPOSE:To provide an antiinfectant having an antiinfective action, useful for viral diseases and causing no adverse reaction, by containing the alkaline water extract of pine tree seed shells. CONSTITUTION:The seed shells of a pine tree such as black pine tree, red pine tree or GOYOMATSU (a five leaves pine tree), preferably the shells of the edible seeds of the GOYOMATSU, are extracted with an alkaline water such as ammonia water at ordinary or high temperature, followed by performing the filtration, concentration and drying of the extract to provide a powder. The objective agent contains the powder as an active ingredient and is obtained by compounding the powder with a carrier for preparations and subsequently preparing the composition by a conventional method. The agent can be prepared in a shape of tables, granules, powders, injections, etc. The powder of the active ingredient has a slightly characteristic smell and is readily soluble in water and brown. The alkaline water for the extraction is preferably used under a pH of >=7 in an amount of approximately five times that of the seed shell of the pine tree.

Description

DETAILED DESCRIPTION OF THE INVENTION (Industrial Field of Application) The present invention relates to an anti-infective agent obtained from botanical substances.

(Prior Art) At present, various compounds have been proposed as anti-infective agents, and none have been developed as pharmaceuticals, and nothing definitive has been obtained in terms of effectiveness, side effects, etc. By the way, some of these anti-infective agents, such as Krestin, were developed from plant ingredients. Based on this, the present inventors have conducted intensive studies on the anti-infective effects of various plant components, and have found that the extract extracted from pine nut shells with alkaline water has an effective anti-infective effect. I found out.

(Structure of the Invention) The present invention uses Pinuspar as an active ingredient.
The present invention relates to an anti-infective agent containing an alkaline aqueous extract of pine nut shells such as Viflora 5ieb, et Zucc, etc.

There are various types of pine nuts, such as black pine, red pine, and five-leaf pine, and any pine nuts can be used in the present invention. However, it is particularly preferable to use Goyo-pine, which produces edible pine nuts.

The harvesting time of the pine nuts used in the present invention is not particularly limited, but it is preferable to pick them around mid-November as they are considered to be rich in components.

Pine nut shell separates pine nuts from pine cones.

Collect the shell that covers the outside of the fruit. The shells may be dried appropriately if necessary and ground to a particle size suitable for extraction.

As alkaline water for extracting components, organic bases,
Alkaline water with any inorganic base may be used. However, from the viewpoint of subsequent processing and preparation of the extract and the extract, it is preferable to use a solution such as aqueous ammonia whose alkali content can be easily removed by heating or the like.

The alkalinity (ALL) of the alkaline water may vary depending on the type and amount of pine nuts, or may be 9117 or higher, preferably about 7.5 to IO.

Extraction is carried out by soaking pine nut shells crushed into appropriate sizes in alkaline water.

The amount of alkaline water used for extraction is not particularly limited, or may be about 5 times the amount of pine nut shells.

The temperature of the alkaline water may be at room temperature or may be heated.

The extract is separated from the pine nut shells by means such as filtration, concentrated to an appropriate concentration by means such as vacuum concentration, adjusted to neutral or slightly acidic with an appropriate acid such as hydrochloric acid, and then frozen. Dry by drying or air drying to obtain the desired product as a powder.

The resulting powder is a brown powder, easily soluble in water, with a slightly characteristic odor.

The active ingredient of the present invention can be formulated into a solid preparation such as a tablet, granule, or powder, or a liquid preparation such as an injection solution, together with a suitable solid or liquid carrier acceptable for drug preparation.

(Example and Test Example) An example of the present invention will be shown below, but the present invention is not limited thereto.

A method previously proposed by one of the inventors (
(Japanese Patent Application Laid-Open No. 171551/1983) to obtain pine nut shells.

0.8% ammonia water 6 to 1 kg of obtained pine nut shells
The mixture was extracted by stirring at 45°C for 3 hours. After separating the extract, the residue was extracted with aqueous ammonia in the same manner once again, and the extracts were combined and filtered through a filter cloth. The filtrate (9.5 sentences) was concentrated under reduced pressure at 40°C to a concentration of 1501.
The pi was adjusted to 5.4 with hydrochloric acid and freeze-dried to obtain 45 g of brown powder.

This powder obtained has a slightly characteristic odor,
Sono 1% (W/V) aqueous solution pH is 4.5-6.0
There was. The content of arsenic and heavy metals in 1 g of powder was 2 pp (less than 30 pp), respectively.

Coliform bacteria was negative, and the general bacterial count was less than t000/g.

An ampoule for injection was prepared as follows.

That is, add 10 g of the obtained powder to about 851 g of water, and add 50 g of the powder.
Stir for 30 minutes at a temperature of ~60°C to dissolve. Add 0.7 g of common salt and water to the solution.

The total amount was set to 1001. Rinse this liquid at 10,000 rp■, 2
Insoluble matter was removed by centrifugation for 0 minutes, and after high-pressure sterilization in an autoclave, the mixture was aseptically filled into ampoules to obtain an injection solution.

1) of the ball.

(1) Big I'! Effect test on SVi infection 0.25
After administering 1 g of powder or saline (control) once into the peritoneal cavity of each group of 10 to 12 ddy mice (5 degenerates, male), on the 20th day, approximately 4xlO6 E. coli bacteria were transplanted into the peritoneal cavity.
The number of surviving mice at 4 hours is calculated.

(2) Efficacy test against viral infections The above injection solution was administered intramuscularly to the thigh of cats suffering from various viral diseases, or between the shoulder bones on the back, as many times as instructed.
0 g/kg was administered, and the general symptoms and the number of white blood cells in the blood were measured over time.

(Action) The anti-infective activity of the aqueous ammonia extract of pine nut shells (powder and injection solution) obtained by the above method was shown to be effective in inhibiting E. coli infection in mice and in cats with viral diseases. The study was conducted using symptom relief as an indicator.

(1) Effect on E. coli infection Table - As shown in Figure 4, pre-treatment of mouse abdominal cavity with aqueous ammonia extract (powder dissolved in physiological saline) prevents E. coli infection in mice. significantly suppressed.

Table-I Effect of aqueous ammonia extract of pine nut shell (powder) on preventing Escherichia coli infection Administrative agent Number of surviving mice 5 days after administration Pine nut shell alkaline water extract II A 7/10 (70X)
B S/10 (5(H) Physiological saline (control) 1/12 (8%) (2
) Effective against viral infections - As shown in H, an aqueous ammonia extract of pine nut shells can be injected subcutaneously into the back near the shoulder bone or intramuscularly into the nub. Viral diseases of cats and dogs (caliciviral glossitis, stomatitis, gingivitis,
It was highly effective against parvovirus-induced feline panleukopenia and herpesvirus rhinobronchitis), with appetite improved, general symptoms improved, and complete recovery in some cases. On the other hand, it was ineffective against diseases that were not caused by viruses (breast cancer, fibrosarcoma).

(Effects of the Invention) The anti-infective agent of the present invention exhibits an effective anti-infective effect and not only suppresses E. coli infection in mice, but is also particularly effective against viral diseases in animals (cats, dogs). It turned out that. This has been demonstrated by the alkaline aqueous extract of pine cones and pine nut shells, or by in vitro
infection of human T-cells (Itagami et al., Abstracts of the 1st Annual Meeting of the AIDS Research Society, p. 6)
0. Kyoto, 1978) and influenza virus infection of MDCK cells (Mizuta et al., unpublished data) at a concentration of 10 uLg/■l. This confirms our knowledge that it is suppressed. In addition, the anti-infective agent of the present invention does not cause the strong side effects seen with other anti-viruses, so it can be used alone or in combination with other treatments or other anti-viral agents. More effective treatments for viral diseases can be provided.

Claims (1)

[Claims] As an active ingredient, Pinus parvifl
oraSieb. etZucc. An anti-infective agent characterized by containing an alkaline water extract of pine nut shells such as ).