JPH01249198A - Method for inhibiting reduction of sulfate in anaerobic treatment and substance hindering growth of sulfate reducing bacteria - Google Patents
Method for inhibiting reduction of sulfate in anaerobic treatment and substance hindering growth of sulfate reducing bacteriaInfo
- Publication number
- JPH01249198A JPH01249198A JP63077774A JP7777488A JPH01249198A JP H01249198 A JPH01249198 A JP H01249198A JP 63077774 A JP63077774 A JP 63077774A JP 7777488 A JP7777488 A JP 7777488A JP H01249198 A JPH01249198 A JP H01249198A
- Authority
- JP
- Japan
- Prior art keywords
- sulfate
- methane
- reducing bacteria
- bacteria
- growth
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 241000894006 Bacteria Species 0.000 title claims abstract description 72
- 239000000126 substance Substances 0.000 title claims abstract description 33
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 title claims abstract description 25
- 238000000034 method Methods 0.000 title claims abstract description 14
- 238000011282 treatment Methods 0.000 title claims description 18
- 230000002401 inhibitory effect Effects 0.000 title claims description 16
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 claims abstract description 98
- 238000000855 fermentation Methods 0.000 claims abstract description 26
- 239000002351 wastewater Substances 0.000 claims abstract description 25
- -1 sulfide ions Chemical class 0.000 claims abstract description 11
- RLSSMJSEOOYNOY-UHFFFAOYSA-N m-cresol Chemical compound CC1=CC=CC(O)=C1 RLSSMJSEOOYNOY-UHFFFAOYSA-N 0.000 claims abstract description 6
- OXNIZHLAWKMVMX-UHFFFAOYSA-N picric acid Chemical compound OC1=C([N+]([O-])=O)C=C([N+]([O-])=O)C=C1[N+]([O-])=O OXNIZHLAWKMVMX-UHFFFAOYSA-N 0.000 claims abstract description 4
- 229950002929 trinitrophenol Drugs 0.000 claims abstract description 4
- QWVGKYWNOKOFNN-UHFFFAOYSA-N o-cresol Chemical compound CC1=CC=CC=C1O QWVGKYWNOKOFNN-UHFFFAOYSA-N 0.000 claims abstract 4
- DOQPXTMNIUCOSY-UHFFFAOYSA-N [4-cyano-4-(3,4-dimethoxyphenyl)-5-methylhexyl]-[2-(3,4-dimethoxyphenyl)ethyl]-methylazanium;chloride Chemical compound [H+].[Cl-].C1=C(OC)C(OC)=CC=C1CCN(C)CCCC(C#N)(C(C)C)C1=CC=C(OC)C(OC)=C1 DOQPXTMNIUCOSY-UHFFFAOYSA-N 0.000 claims description 13
- 229960000881 verapamil hydrochloride Drugs 0.000 claims description 13
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims description 12
- 238000006722 reduction reaction Methods 0.000 claims description 12
- AKUVRZKNLXYTJX-UHFFFAOYSA-N 3-benzylazetidine Chemical compound C=1C=CC=CC=1CC1CNC1 AKUVRZKNLXYTJX-UHFFFAOYSA-N 0.000 claims description 3
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 claims description 3
- 229960001657 chlorpromazine hydrochloride Drugs 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 3
- JOXIMZWYDAKGHI-UHFFFAOYSA-N toluene-4-sulfonic acid Chemical compound CC1=CC=C(S(O)(=O)=O)C=C1 JOXIMZWYDAKGHI-UHFFFAOYSA-N 0.000 claims 2
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims 1
- 229910052708 sodium Inorganic materials 0.000 claims 1
- 239000011734 sodium Substances 0.000 claims 1
- 230000004151 fermentation Effects 0.000 abstract description 18
- 239000001257 hydrogen Substances 0.000 abstract description 6
- 229910052739 hydrogen Inorganic materials 0.000 abstract description 6
- 239000002699 waste material Substances 0.000 abstract description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 abstract description 3
- KVCGISUBCHHTDD-UHFFFAOYSA-M sodium;4-methylbenzenesulfonate Chemical compound [Na+].CC1=CC=C(S([O-])(=O)=O)C=C1 KVCGISUBCHHTDD-UHFFFAOYSA-M 0.000 abstract description 3
- SGTNSNPWRIOYBX-UHFFFAOYSA-N 2-(3,4-dimethoxyphenyl)-5-{[2-(3,4-dimethoxyphenyl)ethyl](methyl)amino}-2-(propan-2-yl)pentanenitrile Chemical compound C1=C(OC)C(OC)=CC=C1CCN(C)CCCC(C#N)(C(C)C)C1=CC=C(OC)C(OC)=C1 SGTNSNPWRIOYBX-UHFFFAOYSA-N 0.000 abstract 1
- GHXZTYHSJHQHIJ-UHFFFAOYSA-N Chlorhexidine Chemical compound C=1C=C(Cl)C=CC=1NC(N)=NC(N)=NCCCCCCN=C(N)N=C(N)NC1=CC=C(Cl)C=C1 GHXZTYHSJHQHIJ-UHFFFAOYSA-N 0.000 abstract 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 abstract 1
- 230000015572 biosynthetic process Effects 0.000 abstract 1
- FBSMERQALIEGJT-UHFFFAOYSA-N chlorpromazine hydrochloride Chemical compound [H+].[Cl-].C1=C(Cl)C=C2N(CCCN(C)C)C3=CC=CC=C3SC2=C1 FBSMERQALIEGJT-UHFFFAOYSA-N 0.000 abstract 1
- 125000004435 hydrogen atom Chemical class [H]* 0.000 abstract 1
- 229960001722 verapamil Drugs 0.000 abstract 1
- 239000007789 gas Substances 0.000 description 15
- 238000012360 testing method Methods 0.000 description 15
- 239000002609 medium Substances 0.000 description 10
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 8
- 238000012216 screening Methods 0.000 description 8
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 6
- 229910000037 hydrogen sulfide Inorganic materials 0.000 description 6
- 230000031068 symbiosis, encompassing mutualism through parasitism Effects 0.000 description 6
- RWSOTUBLDIXVET-UHFFFAOYSA-N Dihydrogen sulfide Chemical compound S RWSOTUBLDIXVET-UHFFFAOYSA-N 0.000 description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 229910052742 iron Inorganic materials 0.000 description 4
- 239000001569 carbon dioxide Substances 0.000 description 3
- 229910002092 carbon dioxide Inorganic materials 0.000 description 3
- 230000006378 damage Effects 0.000 description 3
- 238000012544 monitoring process Methods 0.000 description 3
- 238000004065 wastewater treatment Methods 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 241001074903 Methanobacteria Species 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 239000010802 sludge Substances 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- QTWJRLJHJPIABL-UHFFFAOYSA-N 2-methylphenol;3-methylphenol;4-methylphenol Chemical compound CC1=CC=C(O)C=C1.CC1=CC=CC(O)=C1.CC1=CC=CC=C1O QTWJRLJHJPIABL-UHFFFAOYSA-N 0.000 description 1
- CYDQOEWLBCCFJZ-UHFFFAOYSA-N 4-(4-fluorophenyl)oxane-4-carboxylic acid Chemical compound C=1C=C(F)C=CC=1C1(C(=O)O)CCOCC1 CYDQOEWLBCCFJZ-UHFFFAOYSA-N 0.000 description 1
- 241000186538 Desulfotomaculum nigrificans Species 0.000 description 1
- MBMLMWLHJBBADN-UHFFFAOYSA-N Ferrous sulfide Chemical compound [Fe]=S MBMLMWLHJBBADN-UHFFFAOYSA-N 0.000 description 1
- 241000205286 Methanosarcina sp. Species 0.000 description 1
- 229910000831 Steel Inorganic materials 0.000 description 1
- 238000005273 aeration Methods 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- VNWKTOKETHGBQD-YPZZEJLDSA-N carbane Chemical compound [10CH4] VNWKTOKETHGBQD-YPZZEJLDSA-N 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003638 chemical reducing agent Substances 0.000 description 1
- 230000007797 corrosion Effects 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 229930003836 cresol Natural products 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000004821 distillation Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- IMBKASBLAKCLEM-UHFFFAOYSA-L ferrous ammonium sulfate (anhydrous) Chemical compound [NH4+].[NH4+].[Fe+2].[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O IMBKASBLAKCLEM-UHFFFAOYSA-L 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 150000002431 hydrogen Chemical class 0.000 description 1
- 238000011221 initial treatment Methods 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000001568 sexual effect Effects 0.000 description 1
- 229940005581 sodium lactate Drugs 0.000 description 1
- 239000001540 sodium lactate Substances 0.000 description 1
- 235000011088 sodium lactate Nutrition 0.000 description 1
- 239000010959 steel Substances 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel
Landscapes
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Treatment Of Sludge (AREA)
Abstract
Description
【発明の詳細な説明】
「産業上の利用分野」
この発明は、メタン発酵等の嫌気性発酵によって有機性
廃水を処理する場合に行なわれる嫌気性処理における硫
酸還元反応抑制方法および硫酸還元菌阻害性物質に関す
るものである。Detailed Description of the Invention "Field of Industrial Application" This invention relates to a method for inhibiting a sulfate reduction reaction in anaerobic treatment performed when organic wastewater is treated by anaerobic fermentation such as methane fermentation, and a method for inhibiting sulfate-reducing bacteria. It concerns sexual substances.
「従来の技術」
メタン発酵などの嫌気性発酵による有機性廃水の処理は
、活性゛汚泥法等の好気性処理に比べて、■曝気動力が
不用、■汚泥生成潰が少ない、■エネルギー源として利
用できるメタンガスが生成する、■高負荷の一次処理が
できる、等の利点を持つ水処理法であるが、この方法は
特に有機物を高濃度に含む廃水の処理に適している。"Conventional technology" Compared to aerobic treatments such as activated sludge methods, the treatment of organic wastewater through anaerobic fermentation such as methane fermentation: ■ requires no aeration power; ■ produces less sludge; and ■ can be used as an energy source. This water treatment method has the advantages of producing usable methane gas and being able to perform high-load primary treatment, but this method is particularly suitable for treating wastewater containing high concentrations of organic matter.
「発明が解決しようとする課題」
しかしながら、上記のような高濃度の有機性廃水のなか
には、アルコール恭留廃液、パルプ廃液等のように、し
ばしば多潰の硫酸イオンを含むものがある。このような
廃水をメタン発酵させると、メタン細菌と同様の生育条
件下で硫酸還元菌が繁殖し、硫酸イオンがメタン細菌の
生育およびメタン発酵を阻害する硫化物イオンに還元さ
れるため、メタン発酵が抑制されてメタン生成量、廃水
処理能力が低下し、著しい場合には、メタン発酵が停止
する。また、本来、メタン生成に使われる水素が硫酸イ
オンの還元に使われるため、生成ガス中のメタン濃度が
低下し、生成ガスのほとんどが炭酸ガスとなってエネル
ギー源としての価値が低下する。さらに、生成ガス中に
腐食性の硫化水素ガスの濃度が増加する。``Problems to be Solved by the Invention'' However, among the above-mentioned highly concentrated organic wastewaters, some, such as alcohol distillate wastewater and pulp wastewater, often contain large amounts of sulfate ions. When such wastewater is subjected to methane fermentation, sulfate-reducing bacteria proliferate under the same growth conditions as methane bacteria, and sulfate ions are reduced to sulfide ions that inhibit the growth of methane bacteria and methane fermentation, resulting in methane fermentation. is suppressed, resulting in a decrease in methane production and wastewater treatment capacity, and in severe cases, methane fermentation stops. Additionally, since the hydrogen normally used to generate methane is used to reduce sulfate ions, the methane concentration in the generated gas decreases, and most of the generated gas becomes carbon dioxide, reducing its value as an energy source. Additionally, the concentration of corrosive hydrogen sulfide gas in the product gas increases.
このため、嫌気性処理を行なう場１合には、廃水中の硫
酸還元菌の生育を阻害することによって硫酸イオンの還
元を抑制することが必要と考えられており、そのために
上記廃水中に硫酸還元菌を阻害する物質を添加すること
し考えられるが、従来は、そのような物質がメタン細菌
に及ぼず影響が知られていなかったため、嫌気性処理に
おいて廃水中に硫酸還元菌を阻害する物質を添加する試
みは未だ行なわれたことがなかった。Therefore, when performing anaerobic treatment, it is considered necessary to suppress the reduction of sulfate ions by inhibiting the growth of sulfate-reducing bacteria in the wastewater. One possibility is to add a substance that inhibits sulfate-reducing bacteria, but in the past, it was not known that such substances had any effect on methane bacteria, so adding substances that inhibit sulfate-reducing bacteria to wastewater during anaerobic treatment No attempt has been made to add .
この発明は、上記事情に鑑みてなされたもので、硫酸還
元菌の生育を阻害すると共にメタン細菌の生育を阻害し
ない物質を提供し、かつ、その物質により廃水中の硫酸
還元反応を抑制して嫌気性処理を良好な状態で行なうこ
とを目的としている。This invention was made in view of the above circumstances, and provides a substance that inhibits the growth of sulfate-reducing bacteria and does not inhibit the growth of methane bacteria, and also suppresses the sulfuric acid reduction reaction in wastewater with the substance. The purpose is to perform anaerobic treatment in good conditions.
「課題を解決するための手段」
この発明の嫌気性処理における硫酸還元反応抑制方法は
、硫酸イオンを含む有機性廃水を嫌気性発酵によって処
理する際に、上記有機性廃水にメタン細菌と共存゛する
硫酸還元菌の生育を選択的に阻害する物質を添加するし
のである。"Means for Solving the Problem" The method for suppressing sulfuric acid reduction reaction in anaerobic treatment of the present invention is such that when organic wastewater containing sulfate ions is treated by anaerobic fermentation, methane bacteria coexist with the organic wastewater. This involves adding a substance that selectively inhibits the growth of sulfate-reducing bacteria.
また、この発明の硫酸還元菌阻害性物質は、ベラパミル
塩酸塩、クロルプロマジン塩酸塩、吋クレゾール、ｍ−
クレゾール、ヒビテン、トリニトロフェノール、ｐ−ト
ルエンスルポン酸ナトリウム、グルタルアルデヒドから
選ばれたうちの一種類または二種類以上の混合物からな
るものである。In addition, the sulfate-reducing bacteria-inhibiting substances of this invention include verapamil hydrochloride, chlorpromazine hydrochloride, x-cresol, m-
It consists of one type or a mixture of two or more types selected from cresol, hibitene, trinitrophenol, sodium p-toluenesulfonate, and glutaraldehyde.
「作用」
この発明の嫌気性処理における硫酸還元反応抑制方法に
おいては、硫酸イオンを含む有機性廃水を嫌気性発酵に
よって処理する際に、上記有機性廃水にメタン細菌と共
存する硫酸還元菌の生育を選択的に阻害する物質を添加
することによって、上記硫酸還元菌による硫酸イオンの
還元を抑制してメタン発酵を阻害する硫化物イオンの発
生を防ぐと共に上記硫酸還元菌がメタン生成に必要な水
素を消費することも防止する。このため、この方法を用
いて有機性廃水の嫌気性処理を行なうと、メタン発酵が
良好な状態で行なわれて、廃水処理が効果的に行なわれ
ることとなる。"Operation" In the sulfate reduction reaction suppression method in anaerobic treatment of the present invention, when organic wastewater containing sulfate ions is treated by anaerobic fermentation, sulfate-reducing bacteria coexisting with methane bacteria grow in the organic wastewater. By adding a substance that selectively inhibits sulfate ions, the reduction of sulfate ions by the sulfate-reducing bacteria is suppressed, thereby preventing the generation of sulfide ions that inhibit methane fermentation, and also preventing the sulfate-reducing bacteria from producing the hydrogen necessary for methane production. It also prevents consumption of Therefore, when organic wastewater is treated anaerobically using this method, methane fermentation is carried out in good conditions, and wastewater treatment is effectively carried out.
また、この発明の硫酸還元菌阻害性物質を用いた場合に
は、有機性廃水の嫌気性処理を効率的に行なうことがで
きる上、その硫酸還元菌阻害性によって、鉄製のタンク
、配管等が硫酸還元菌がつくりだす硫化水素ガスで腐食
されることも防止することができ、硫酸還元菌による被
害に対して広く応用することが可能である。Furthermore, when the sulfate-reducing bacteria-inhibiting substance of the present invention is used, it is possible to efficiently perform anaerobic treatment of organic wastewater, and due to its sulfate-reducing bacteria-inhibiting property, iron tanks, piping, etc. It can also prevent corrosion caused by hydrogen sulfide gas produced by sulfate-reducing bacteria, and can be widely applied to damage caused by sulfate-reducing bacteria.
「実施例」 以下、この発明の一実施例を説明する。"Example" An embodiment of this invention will be described below.
この実施例では、例えばアルコール蒸留廃液あるいはバ
ルブ廃液等のように硫酸イオンを含む有機性廃水中に、
硫酸還元菌の生育を阻害すると共にメタン細菌の生育を
阻害しない硫酸還元菌阻害性物質を添加し、そのＨ機性
廃水をメタン発酵させて嫌気性処理を行なう。In this example, in organic wastewater containing sulfate ions, such as alcohol distillation waste liquid or valve waste liquid,
A sulfate-reducing bacteria-inhibiting substance that inhibits the growth of sulfate-reducing bacteria and does not inhibit the growth of methane bacteria is added, and the H organic wastewater is subjected to methane fermentation to perform anaerobic treatment.
このような硫酸還元菌阻害性物質としては、例えばベラ
パミル塩酸塩などがある。そして、このベラパミル塩酸
塩を例えば１０ｐｐｍ程度の適当な濃度になるようにし
て発酵槽（あるいはりアクタ−１消化槽等）に添加する
と、ベラパミル塩酸塩の作用により硫酸還元菌の活動か
抑制されるため、硫酸イオンの硫化物イオンへの還元が
起こらず、メタン発酵を良好な状態で行なうことができ
る。Examples of such sulfate-reducing bacteria-inhibiting substances include verapamil hydrochloride. When this verapamil hydrochloride is added to a fermenter (or an Actor-1 digester, etc.) at an appropriate concentration of, for example, 10 ppm, the activity of sulfate-reducing bacteria is suppressed by the action of verapamil hydrochloride. Therefore, reduction of sulfate ions to sulfide ions does not occur, and methane fermentation can be carried out in good conditions.
また、上記ベラパミル塩酸塩のような硫酸還元菌阻害性
物質は、硫酸還元菌による腐食性の硫化水素ガスの発生
も抑制できるため、反応処理における発酵槽、リアクタ
ー、または消化槽等ばかりでなく、土中等の嫌気下にお
ける鉄製の送油管、ガス管、および水道管等が硫化水素
ガスで腐食されることら防止することもできる。In addition, sulfate-reducing bacteria-inhibiting substances such as verapamil hydrochloride can also suppress the generation of corrosive hydrogen sulfide gas by sulfate-reducing bacteria, so they can be used not only in fermenters, reactors, or digesters in reaction treatments, but also in It is also possible to prevent steel oil pipes, gas pipes, water pipes, etc. from being corroded by hydrogen sulfide gas under anaerobic conditions such as soil.
なお、上記硫酸還元菌阻害性物質としては、上記ベラパ
ミル塩酸塩の他、クロルプロマジン塩酸塩、０−クレゾ
ール、ｍ−クレゾール、ヒビテン、トリニトロフェノー
ル、ｐ−トルエンスルホン酸ナトリウム、グルタルアル
デヒドおよびその他の物質などがある。そして、これら
の物質を用いた場合にも、ベラパミル塩酸塩を用いた場
合と同様な効果を奏することができる。In addition to the above-mentioned verapamil hydrochloride, the above-mentioned sulfate-reducing bacteria-inhibiting substances include chlorpromazine hydrochloride, 0-cresol, m-cresol, hibitene, trinitrophenol, sodium p-toluenesulfonate, glutaraldehyde, and other substances. and so on. Even when these substances are used, the same effects as when verapamil hydrochloride is used can be achieved.
「実験例」
（実験ｌ ）スクリーニングテスト
硫酸還元菌の生育を阻害し、メタン細菌の生育を１ｌｆ
ｌ害しない物質を捜ずため、表１に示すフローに基づい
てスクリーニングテストを実施した。"Experiment example" (Experiment 1) Screening test Inhibits the growth of sulfate-reducing bacteria and inhibits the growth of methane bacteria by 1lf
In order to search for substances that do not cause any harm, a screening test was conducted based on the flow shown in Table 1.
このスクリーニングテストにおいては、メタン細菌とし
て、メタノールを炭素源として利用可能で、しかもその
メタン発酵域の至適生育温度が５５℃のメタノザルシナ
（Ｍｅｔｈａｎｏｓａｒｃｉｎａ ｓｐ、（ＤＳＭ２９
０６））を用い、硫酸還元菌として、上記至適生育温度
とほぼ等しい至適生育温度を持つデスルホトマクルムニ
グリフイカンス（Ｄｅｓｕｌｕｆｏｔｏｍａｃｕｌｕｍ
ｎｉｇｒｉｆｉａａｎｓ （ＤＳＭ ５７４））を用い
た。そして、これら各画の菌株に対して、表２、表３に
示す培地を調整した。In this screening test, Methanosarcina sp, (DSM29
06)) was used as a sulfate-reducing bacterium, Desulfotomaculum nigrificans, which has an optimal growth temperature almost equal to the above optimal growth temperature.
nigrifiaans (DSM 574)). Then, the culture media shown in Tables 2 and 3 were prepared for each of these bacterial strains.
表１゜
（以下余白）
表２．硫酸還元菌生育確認用培地
但し、上記培地はオートクレーブにより １２０℃で１
５分間殺菌処理され、また、そのｐＨは７．２に調整さ
れている。そして、このスクリーニングテストは、窒素
８０％、二酸化炭素ｌＯ％、水素ＩＱ％の雰囲気中にお
いて行なった。Table 1゜ (margins below) Table 2. Medium for confirming the growth of sulfate-reducing bacteria. However, the above medium is heated to 120°C by autoclaving.
It was sterilized for 5 minutes and its pH was adjusted to 7.2. This screening test was conducted in an atmosphere of 80% nitrogen, 10% carbon dioxide, and IQ% hydrogen.
（以下余白）
表３゜メタン細菌生育確認用培地
但し、上記培地はオートクレーブにより １２０℃で１
５分間殺菌処理され、また、そのｐＨは７．２に調整さ
れている。そして、このスクリーニングテストは、窒素
８０％、二酸化炭素１０％、水素１０％の雰囲気中にお
いて行なった。(Left below) Table 3: Medium for confirming the growth of methane bacteria. However, the above medium was heated to 120°C by autoclaving
It was sterilized for 5 minutes and its pH was adjusted to 7.2. This screening test was conducted in an atmosphere of 80% nitrogen, 10% carbon dioxide, and 10% hydrogen.
なお、表３中におけるビタミン溶液および微量元素溶液
の配合をそれぞれ表４、表５に示す。The formulations of the vitamin solution and trace element solution in Table 3 are shown in Table 4 and Table 5, respectively.
表４゜
表５゜
このようなスクリーニングテストにおいて、培地に硫酸
還元菌が生存、生育する場合には、硫酸塩が乳酸ナトリ
ウムを還元剤として還元され、モール塩中の鉄と作用し
て硫化鉄を生成し、培地が黒変する。このため、このス
クリーニングテストでは、培地゛の黒変によって硫酸還
元菌の生育確認とした。また、メタン細菌は、生育する
と、培養液の入った試験管の下部に根粒状となって沈澱
するため、培養液は濁らない。さらに、メタン細菌の生
育に伴ってメタンガスが発生するので、これをもって生
育確認とする。Table 4゜Table 5゜In such a screening test, if sulfate-reducing bacteria survive and grow in the medium, sulfate is reduced using sodium lactate as a reducing agent, and interacts with iron in Mohr's salt to produce iron sulfide. , and the medium turns black. Therefore, in this screening test, the growth of sulfate-reducing bacteria was confirmed by the blackening of the medium. Furthermore, when methane bacteria grow, they precipitate in the form of nodules at the bottom of the test tube containing the culture solution, so the culture solution does not become cloudy. Furthermore, as methane bacteria grow, methane gas is generated, which is used to confirm growth.
そして、このようにして多数の物質についてスクリーニ
ングテストを行った結果、表６に示す８種の物質が有効
と認められた。As a result of conducting screening tests on a large number of substances in this manner, eight substances shown in Table 6 were recognized as effective.
なお、各物質名の右側に記載した有効濃度とは、メタン
発酵が最も良好な状態で行なわれる濃度であるが、これ
以外の濃度でも硫酸還元菌の生育を抑制することが可能
であり、その場合にもメタン発酵を効率的に行なうこと
ができる。The effective concentration listed to the right of each substance name is the concentration at which methane fermentation occurs in the best conditions, but it is also possible to suppress the growth of sulfate-reducing bacteria at other concentrations. In some cases, methane fermentation can be carried out efficiently.
表６゜
（実験２ ）共生テスト
実験ｌで有効と認められた表６の物質のうちからベラパ
ミル塩酸塩を選び、表７の培地を用いて共生テストを行
った。Table 6 (Experiment 2) Symbiosis Test Verapamil hydrochloride was selected from among the substances in Table 6 that were found to be effective in Experiment 1, and a symbiosis test was conducted using the medium in Table 7.
この共生テストでは、実験ｌと同様な実験方法で行なわ
れ、メタン細菌の単独培養、メタン細菌と硫酸還元菌と
の混合培養、および上記ベラパミル塩酸塩をｔｏｐβｍ
添加したメタン細菌と硫酸還元菌との混合培養を行なっ
た。そして、メタン細菌の増殖は、メタンガス発生量を
モニターすることにより検知し、硫酸還元菌の増殖は、
硫酸イオン濃度の変化をモニターすることにより検知し
た。This symbiosis test was carried out using the same experimental methods as Experiment 1, including a single culture of methanobacteria, a mixed culture of methanobacteria and sulfate-reducing bacteria, and the above verapamil hydrochloride was added to topβm.
A mixed culture of the added methane bacteria and sulfate-reducing bacteria was performed. The growth of methane bacteria is detected by monitoring the amount of methane gas generated, and the growth of sulfate-reducing bacteria is detected by monitoring the amount of methane gas generated.
Detection was made by monitoring changes in sulfate ion concentration.
（以下余白） 表７゜ 但し、上記培地は、ｐＨｈ（７，０に調整されている。(Margin below) Table 7゜ However, the above medium was adjusted to pHh (7.0).
この共生テストの結果、第１図、第２図に示すように、
メタン細菌と硫酸還元菌とを混合した系にベラパミル塩
酸塩を添加した場合には、メタン細菌単独の系と同様に
良好なメタン発酵が行なわれ、かつ硫酸還元菌の増殖も
抑制された。As a result of this symbiosis test, as shown in Figures 1 and 2,
When verapamil hydrochloride was added to a mixed system of methane bacteria and sulfate-reducing bacteria, methane fermentation was performed as well as in the system with methane bacteria alone, and the growth of sulfate-reducing bacteria was also suppressed.
また、上記共生テストと同様に表７の培地を用いて、上
記表６中に記載した上記ベラパミル塩酸塩以外の各物質
゛を添加したメタン細菌と硫酸還元菌との混合培養を行
なったところ、これら各物質を添加した場合にも、上記
ベラパミル塩酸塩を添加した場合と同様に良好なメタン
発酵が行なわれ、かつ硫酸還元菌の増殖も抑制された。In addition, as in the symbiosis test above, a mixed culture of methane bacteria and sulfate-reducing bacteria was carried out using the medium shown in Table 7 to which each substance other than verapamil hydrochloride listed in Table 6 was added. When each of these substances was added, methane fermentation was carried out as well as in the case where verapamil hydrochloride was added, and the growth of sulfate-reducing bacteria was also suppressed.
「発明の効果」
この発明の嫌気性処理における硫酸還元反応抑制方法に
よれば、硫酸イオンを含む有機性廃水を嫌気性発酵によ
って処理する際に、上記有機性廃水にメタン細菌と共存
する硫酸還元菌の生育を選択的に阻害する物質を添加す
るので、上記硫酸還元菌による硫酸イオンの還元を抑制
してメタン発酵を阻害する硫化物イオンの発生を防ぐこ
とができると共に、メタン生成に必要な水素が上記硫酸
還元菌に消費されることを防止することができる。"Effects of the Invention" According to the method for suppressing sulfuric acid reduction reaction in anaerobic treatment of the present invention, when organic wastewater containing sulfate ions is treated by anaerobic fermentation, sulfuric acid reduction coexists with methane bacteria in the organic wastewater. Since a substance that selectively inhibits the growth of bacteria is added, it is possible to suppress the reduction of sulfate ions by the sulfate-reducing bacteria and prevent the generation of sulfide ions that inhibit methane fermentation. It is possible to prevent hydrogen from being consumed by the sulfate-reducing bacteria.
このため、この方法を用いて有機性廃水の嫌気性処理を
行なった場合には、メタン発酵を良好な状態で行なうこ
とができ、廃水処理を効果的に行なうことができる。Therefore, when organic wastewater is anaerobically treated using this method, methane fermentation can be carried out in good conditions, and wastewater treatment can be carried out effectively.
ま、た、この発明の硫酸還元菌阻害性物質によれば、硫
酸還元菌の生育を阻害すると共にメタン細菌の生育を阻
害しないために嫌気性処理を効率的に行なうことができ
るだけでなく、その硫酸還元菌阻害性によって、その嫌
気性処理で用いられる鉄製のタンク、配管などが硫酸還
元菌がっくりだす硫化水素ガスで腐食されることも防止
することができる。そして、この硫酸還元菌阻害性物質
によれば、土中等の嫌気したにおける鉄製の送油管、ガ
ス管、水道管などが硫酸還元菌がっくりだす硫化水素イ
オンで腐食されることも防止することができ、その他の
硫酸還元菌による被害に対しても広く応用して幅広い産
業分野に利用することが可能である。Moreover, according to the sulfate-reducing bacteria-inhibiting substance of the present invention, not only can anaerobic treatment be carried out efficiently since it inhibits the growth of sulfate-reducing bacteria and does not inhibit the growth of methane bacteria. The sulfate-reducing bacteria inhibiting property also prevents iron tanks, piping, etc. used in the anaerobic treatment from being corroded by hydrogen sulfide gas released by sulfate-reducing bacteria. In addition, this sulfate-reducing bacteria-inhibiting substance can also prevent iron oil pipes, gas pipes, water pipes, etc. from being corroded by hydrogen sulfide ions released by sulfate-reducing bacteria in anaerobic environments such as soil. It can be widely applied to damage caused by other sulfate-reducing bacteria, and can be used in a wide range of industrial fields.
第１図、第２図は、共生テストの結果を示す図であって
、第１図は培養時間と硫酸イオン濃度との関係を表すグ
ラフ、第２図は培養時間とメタンガス発生ｍとの関係を
表すグラフである。
出廓°大 清水建設株式会社Figures 1 and 2 are diagrams showing the results of the symbiosis test, with Figure 1 being a graph showing the relationship between culture time and sulfate ion concentration, and Figure 2 being a graph showing the relationship between culture time and methane gas generation m. This is a graph representing Shimizu Construction Co., Ltd.
Claims (2)
て処理する際に、上記有機性廃水にメタン細菌と共存す
る硫酸還元菌の生育を選択的に阻害する物質を添加する
ことを特徴とする嫌気性処理における硫酸還元反応抑制
方法。(1) When organic wastewater containing sulfate ions is treated by anaerobic fermentation, a substance that selectively inhibits the growth of sulfate-reducing bacteria that coexists with methane bacteria is added to the organic wastewater. Method for suppressing sulfuric acid reduction reaction in anaerobic treatment.
制方法で添加する物質において、ベラパミル塩酸塩、ク
ロルプロマジン塩酸塩、ｏ−クレゾール、ｍ−クレゾー
ル、ヒビテン、トリニトロフェノール、ｐ−トルエンス
ルホン酸ナトリウム、グルタルアルデヒドから選ばれた
うちの一種類または二種類以上の混合物からなることを
特徴とする硫酸還元菌阻害性物質。(2) Among the substances added in the method for inhibiting sulfuric acid reduction reaction in anaerobic treatment described in paragraph 1, verapamil hydrochloride, chlorpromazine hydrochloride, o-cresol, m-cresol, hibitene, trinitrophenol, p-toluenesulfonic acid A sulfate-reducing bacterium inhibitory substance comprising one or a mixture of two or more selected from sodium and glutaraldehyde.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP63077774A JPH01249198A (en) | 1988-03-30 | 1988-03-30 | Method for inhibiting reduction of sulfate in anaerobic treatment and substance hindering growth of sulfate reducing bacteria |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP63077774A JPH01249198A (en) | 1988-03-30 | 1988-03-30 | Method for inhibiting reduction of sulfate in anaerobic treatment and substance hindering growth of sulfate reducing bacteria |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH01249198A true JPH01249198A (en) | 1989-10-04 |
| JPH0310393B2 JPH0310393B2 (en) | 1991-02-13 |
Family
ID=13643302
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP63077774A Granted JPH01249198A (en) | 1988-03-30 | 1988-03-30 | Method for inhibiting reduction of sulfate in anaerobic treatment and substance hindering growth of sulfate reducing bacteria |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH01249198A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH01268603A (en) * | 1988-04-21 | 1989-10-26 | Shimizu Corp | Method for inhibiting growth of sulfate-reducing bacteria |
| JPH01270997A (en) * | 1988-04-21 | 1989-10-30 | Shimizu Corp | Anaerobic fermentation method and anaerobic fermentation equipment |
| JPH01272502A (en) * | 1988-04-21 | 1989-10-31 | Shimizu Corp | Prevention against growing of sulfuric acid reduction fungi |
| WO2013137322A1 (en) * | 2012-03-13 | 2013-09-19 | 株式会社ダイセル | Waste water treatment method |
| CN106854026A (en) * | 2016-10-28 | 2017-06-16 | 江苏迈达新材料股份有限公司 | It is a kind of to process the method that 2,6 di-tert-butyl methyl phenols produce waste liquid |
-
1988
- 1988-03-30 JP JP63077774A patent/JPH01249198A/en active Granted
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH01268603A (en) * | 1988-04-21 | 1989-10-26 | Shimizu Corp | Method for inhibiting growth of sulfate-reducing bacteria |
| JPH01270997A (en) * | 1988-04-21 | 1989-10-30 | Shimizu Corp | Anaerobic fermentation method and anaerobic fermentation equipment |
| JPH01272502A (en) * | 1988-04-21 | 1989-10-31 | Shimizu Corp | Prevention against growing of sulfuric acid reduction fungi |
| WO2013137322A1 (en) * | 2012-03-13 | 2013-09-19 | 株式会社ダイセル | Waste water treatment method |
| JPWO2013137322A1 (en) * | 2012-03-13 | 2015-08-03 | 株式会社ダイセル | Wastewater treatment method |
| CN106854026A (en) * | 2016-10-28 | 2017-06-16 | 江苏迈达新材料股份有限公司 | It is a kind of to process the method that 2,6 di-tert-butyl methyl phenols produce waste liquid |
| CN106854026B (en) * | 2016-10-28 | 2020-11-20 | 江苏迈达新材料股份有限公司 | Method for treating 2, 6-di-tert-butyl-p-methylphenol production waste liquid |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0310393B2 (en) | 1991-02-13 |
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