JPH0138762B2 - - Google Patents

Description

[Detailed description of the invention]

This invention relates to cosmetics suitable for the beauty and health of skin and hair, containing polysaccharides present in medicinal ginseng as active ingredients. Medicinal ginseng, especially Araliaceae
Panax ginseng (Panax ginseng, C.A. Meyer, Panax ginseng C.
A. MEYER), also known as Panax ginseng, is widely known to have been used as a tonic, tonic, anti-inflammatory, diuretic, blood pressure lowering, and anti-diabetic drug since ancient times. Recently, research has been underway to investigate whether these medicinal effects may be due to the saponin components in Panax ginseng. The inventors of this invention arrived at this invention by discovering that medicinal ginseng contains not a saponin component but a polysaccharide whose existence was previously unknown, and that this polysaccharide has a skin revitalizing effect. This is what I did. The properties of the obtained polysaccharide vary slightly depending on the type of medicinal ginseng used as the raw material, but all have the following properties. (i) Has a skin activating effect (ii) Soluble in water, sparingly soluble in methanol, ethanol,
Insoluble in ether, benzene, chloroform and acetone (iii) PH of 1% aqueous solution is approximately 5.5 (iv) Decomposition temperature approximately 218℃ (v) A mixture of 2% phenol aqueous solution and concentrated sulfuric acid exhibits a pale yellow-red color and a silver mirror reaction. Thus, the present invention provides a cosmetic material containing as an active ingredient a polysaccharide contained in medicinal ginseng and characterized by having the above-mentioned properties. Panax ginseng is the most preferred carrot containing polysaccharide in this invention. In addition, there are other green plants such as Panax ginseng (Panax japonicus CAMEYER), American ginseng (Panax quinquefolium LINNE), Panax ginseng (Panax quinquefolium LINNE), Panax notoginseng BURKILL or Panax pseudoginseng, Panax
pseudoginseng WALLICH). As raw materials for the polysaccharide of the present invention, raw, dried, and herbal medicines of the roots and rhizomes of the carrot, and cultured tissue obtained by tissue culturing the carrot can be used. The active ingredient polysaccharide can be obtained as follows. First, the raw material is extracted with water or an aqueous organic solvent (equivalent to 5 to 7 times the amount of the raw material), either undefatted or after defatted using a normal fat-soluble solvent. Although water is sufficient for extraction, an aqueous organic solvent may be used to prevent spoilage of the extract, or extraction may be performed with both. As the organic solvent for the aqueous organic solvent, lower alcohols such as methanol and ethanol, or acetone are used, and depending on the type of raw materials, 50%
Hereinafter, those containing an organic solvent of 30% or less are preferably used. Further, this extraction is promoted by heating, and the raw material is preferably crushed or pulverized. The obtained extract as it is, a concentrated liquid obtained by concentrating this under reduced pressure, or a lower alcohol is added to the extracted liquid or concentrated liquid to precipitate the target substance, which is filtered and washed with ethanol to obtain a precipitate. , using a solution dissolved in water as the inner solution and using a cellulose dialysis membrane (model 36/32 manufactured by Visking) that removes substances with a size of 24 angstroms or less for 6 days using water as the outer solution. and undergo dialysis. Alternatively, the extract obtained by drying the above extract under reduced pressure may be dissolved in water, and the solution obtained by removing insoluble matter may be subjected to dialysis. The dialysis solution obtained as described above is dried under reduced pressure to obtain a light brown-white powder of the polysaccharide of the present invention. Next, an example of producing the polysaccharide of this invention will be described. Example of producing medicinal carrot polysaccharide 1 Cut 10 kg of dried roots of Panax ginseng (4th year old) into small pieces, and extract by immersing them in a mixture of water and methanol (1:1) for 3 days with stirring at room temperature. After performing this extraction twice in total, approximately 60% of the residue
Extract by soaking in water for 3 days while stirring. All the extracts were combined and filtered, and the solvent was distilled off under reduced pressure to give a volume of approx.
Concentrate to 10% (if foaming occurs, add a small amount of n-butanol to defoam it). The obtained concentrate is filtered to remove insoluble matter. This filtrate is placed in a 36/32 type cellulose dialysis membrane manufactured by Visking, and dialysis is performed for 6 days using purified water as an external liquid. The obtained internal fluid
The solvent was distilled off under reduced pressure at 60° C. or lower, and the residue was dried overnight in a desiccator to obtain 152 g of a pale brown-white powder of polysaccharide.
This product is a polysaccharide with the following physical properties,
It has skin revitalizing effects as described below. (i) It is a pale brown to white powder, tasteless and odorless. (ii) Soluble in water, sparingly soluble in methanol, insoluble in ethanol, ether, benzene, chloroform and atone (iii) PH of 1% aqueous solution is approximately 5.5. (iv) Infrared absorption spectrum (KBr method) νmax: 3450, 1745, 1625, 1415, 1350, 1250
and 1020cm ^-1 (v) nuclear magnetic resonance spectrum (90MHz, D ₂ O) 5.21 (s), 3.63 (s), 3.56 (s), 3.49 (s),
3.43 (s) and 3.34 (s) (vi) Decomposition temperature: Approximately 218℃ (vii) Specific rotation [α] ²⁵ _D + 116゜ (c5, water) (viii) Coloration, precipitation reaction a 2 in 1 ml of 1% aqueous solution % phenol aqueous solution 1
When 1 ml of concentrated sulfuric acid is added to the solution, it becomes pale yellow. b Adding 2-3 drops of a 1% aqueous solution to 5 ml of boiling Fehling's reagent produces a red precipitate. c 1% aqueous solution is positive for silver mirror reaction. (ix) Elemental analysis Contains nuclear atoms of the elements, hydrogen, oxygen and nitrogen. Production example of medicinal carrot polysaccharide 2 Cut 10 kg of dried roots of Panax ginseng (fourth year old) into small pieces, and extract by immersing them in about 60 ml of water at room temperature for 3 days with stirring. After performing this extraction three times in total, all extracts are combined and filtered. The filtrate is concentrated to about 10% by distilling off the solvent under reduced pressure (in case of foaming, n
Add a small amount of butanol to defoam). Approximately 100 ml of ethanol was added to the obtained concentrate to precipitate the crude polysaccharide, which was filtered and washed with ethanol from step 3. The solvent was removed under reduced pressure to obtain the crude polysaccharide as a light brown white powder.
Obtained 350g. This polysaccharide is dissolved in 10 purified water and filtered to remove insoluble matter. This filtrate is placed in a 36/32 type cellulose dialysis membrane manufactured by Visking and dialyzed for 6 days using purified water as an external liquid. The obtained internal solution was poured into about 100 ml of ethanol, the precipitate was collected by filtration, and the solvent was distilled off under reduced pressure at a temperature below 60°C.
The mixture was dried in a desiccator overnight to obtain 210 g of a pale brownish white powder polysaccharide. This product has the same physical properties and skin revitalizing effect as the polysaccharide of Production Example 1. The dosage forms of the cosmetics of this invention include skin cosmetics such as lotions, lotions, creams, and packs, hair cosmetics such as shampoos, hair tonics, treatments, and hair creams, oral beauty cosmetics such as syrups, teas, tablets, and soaps. It may be any of the following. As the cosmetically acceptable base used in the cosmetic composition of the present invention, those known in the art can be used. Furthermore, various additives such as fragrances, antioxidants, preservatives, and surfactants may be added to the cosmetics of the present invention as appropriate. Further, the cosmetic composition of the present invention can be manufactured by a preparation method known in the art. The cosmetics of this invention generally contain 0.001 to 3% by weight of the above-mentioned polysaccharides, and suitable ones include
It is added in an amount of 0.01 to 0.5% by weight, more preferably 0.01 to 0.1% by weight. When the cosmetic is especially soap, the polysaccharide is
It is preferable to add 0.01 to 0.5% by weight. Next, examples of various cosmetics containing the medicated ginseng polysaccharide of the present invention as an active ingredient will be shown. In each case, only the prescriptions are described, but cosmetics with these formulations can be manufactured by conventional methods. Example 1 (Lotion) Ethyl alcohol 15.00% Medicated carrot mucilage polysaccharide 0.10 Glycerin 8.00 Propylene glycol 5.00 Polyoxyethylene olein 2.00 Alcohol ether fragrance 0.50 Purified water 69.40 Example 2 (Cream) Medicinal carrot mucilage polysaccharide 0.10% Stearic acid 8.00 Propylene glycol 5.00 Olive oil 5.00 Polyoxyethylene sorbitan 3.00 Isopropyl myristate monostearate 2.00 Fragrance 0.30 Purified water 76.60 Example 3 (Heatnik) Medicinal carrot mucilage polysaccharide 0.05% Ethyl alcohol 55.00 Glycerin 5.00 Polyoxyethylene oleyl 2. 00 Alcohol ether scent Material 0.25 Purified water 37.70 Example 4 (Milk lotion) Medicinal carrot mucilage polysaccharide 0.05% Stearic acid 10.00 Cetyl alcohol 8.00 Glycerin monostearate 8.00 Lanolin 2.00 Polyoxyethylene sorbitan 0.20 Monostearate flavor 0.30 Purified water 71.45 Example 5 (Transparent soap) Medicinal carrot mucilage polysaccharide 0.20% Soap content 60.00 Sucrose 11.50 Glycerin 9.40 Ethyl alcohol 0.90 Fragrance 1.40 Dyes and others 1.10 Purified water 15.50 Next, the effects of using the cosmetic of this invention will be explained. (i) Test method Twenty women each between the ages of 30 and 40 with severe rough skin, those with dark spots, and those with severe itching were selected as test subjects. The cream with the formulation of Example 2 was applied continuously to the rough and blemished skin group twice in the morning and evening for two months, and to the itchy skin group twice in the morning and evening for one month, and then the transparent soap with the formulation of Example 5 was applied. was used once a day during bathing for two months on a rough skin group and a blemish group, and its effects were investigated using the following method. That is, for each symptom, the 5 shown in Table 1 below.
A graded evaluation standard was created, and the symptoms before and after the use test were graded as shown in Table 1.

【table】

[Table] Comparing the symptoms before the test and the symptoms after the test, those with an improvement effect of 3 or more levels are markedly effective, those with a 2 level improvement effect are effective, and those with a 1 level improvement effect. Slightly effective,
The results shown in Tables 2 to 6 below were obtained by counting those that remained unchanged or deteriorated as invalid.

【table】

【table】

【table】

【table】

[Table] (ii) Test effect As shown in the table above, when the cosmetics of this invention are divided into two groups: markedly effective and effective are combined into an effective group, and moderately effective and ineffective are combined into an ineffective group. The cream and transparent soap showed an excellent improvement effect on rough skin, with an effectiveness rate of 65%, including the cosmetically important effect of applying makeup, and even those in the ineffective group were slightly effective in many cases. From this, it is considered that the polysaccharide of the present invention has water-retaining properties that give vitality to the skin and keep it moisturized. Also, creams have a 55% effectiveness rate for itching.
This showed an excellent improvement effect. Furthermore, for stains that are conventionally considered difficult to improve, cream and soap each have 35%
% and showed an efficacy rate of 40%. In addition to the above-mentioned effects, the polysaccharide of the present invention was also found to impart vitality and moisture to the hair. As is clear from the above results, the cosmetic containing medicated ginseng polysaccharide of this invention has excellent beauty effects.

Claims (1)

[Claims] 1. Contained in medicinal ginseng and having the following properties: (i) Has a skin activating effect (ii) Soluble in water, sparingly soluble in methanol, ethanol,
Insoluble in ether, benzene, chloroform and acetone (iii) PH of 1% aqueous solution is approximately 5.5 (iv) Decomposition temperature approximately 218℃ (v) A mixture of 2% phenol aqueous solution and concentrated sulfuric acid exhibits a pale yellow-red color and a silver mirror reaction. 1. A cosmetic containing as an active ingredient a polysaccharide characterized by having a positive result in Fehling's test solution. 2. Medicinal ginseng is Panax ginseng (Panatucus ginseng, C.A. Mayer), Panax ginseng (Panatchus japonicus, C.A. Mayer), American ginseng (Panatux quinquefolium, Linnaeus), Panax ginseng (Panatchus notoginseng, Burkill or 2. The cosmetic composition according to claim 1, wherein the cosmetic composition is Panax pseudoginseng, Warritsch). 3. The cosmetic according to claim 2, wherein the medicinal ginseng is Panax ginseng. 4. The cosmetic according to claim 1, which contains 0.001 to 3% by weight of each saccharide of medicinal ginseng. 5. The cosmetic according to claim 4, which contains 0.01 to 0.5% by weight of medicated ginseng polysaccharide and is in the form of a soap.