JPH0141222B2 - - Google Patents

Info

Publication number: JPH0141222B2
Authority: JP; Japan
Prior art keywords: polypeptide; gel; staining; silver; polypeptides
Prior art date: 1982-01-18
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired

Application number

JP58006552A

Other languages

English (en)

Japanese (ja)

Other versions

JPS58216958A (ja

Inventor

Aaru Meeriru Kaaru

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

US Department of Energy

Original Assignee

US Department of Energy

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1982-01-18

Filing date

1983-01-18

Publication date

1989-09-04

1982-01-18 Priority claimed from US06/339,886 external-priority patent/US4405720A/en

1983-01-18 Application filed by US Department of Energy filed Critical US Department of Energy

1983-12-16 Publication of JPS58216958A publication Critical patent/JPS58216958A/ja

1989-09-04 Publication of JPH0141222B2 publication Critical patent/JPH0141222B2/ja

Status Granted legal-status Critical Current

Links

229920001184 polypeptide Polymers 0.000 claims description 185
102000004196 processed proteins & peptides Human genes 0.000 claims description 185
108090000765 processed proteins & peptides Proteins 0.000 claims description 185
238000000034 method Methods 0.000 claims description 69
QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 56
WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 claims description 52
238000010186 staining Methods 0.000 claims description 48
239000000243 solution Substances 0.000 claims description 43
SQGYOTSLMSWVJD-UHFFFAOYSA-N silver(1+) nitrate Chemical group [Ag+].[O-]N(=O)=O SQGYOTSLMSWVJD-UHFFFAOYSA-N 0.000 claims description 38
208000002109 Argyria Diseases 0.000 claims description 29
LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 26
OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 24
BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 claims description 23
229910052709 silver Inorganic materials 0.000 claims description 23
239000004332 silver Substances 0.000 claims description 23
KMUONIBRACKNSN-UHFFFAOYSA-N potassium dichromate Chemical compound [K+].[K+].[O-][Cr](=O)(=O)O[Cr]([O-])(=O)=O KMUONIBRACKNSN-UHFFFAOYSA-N 0.000 claims description 22
238000007447 staining method Methods 0.000 claims description 22
229910001961 silver nitrate Inorganic materials 0.000 claims description 19
239000000203 mixture Substances 0.000 claims description 15
150000003839 salts Chemical class 0.000 claims description 15
229930040373 Paraformaldehyde Natural products 0.000 claims description 12
239000007800 oxidant agent Substances 0.000 claims description 12
229920002866 paraformaldehyde Polymers 0.000 claims description 12
239000003638 chemical reducing agent Substances 0.000 claims description 11
239000003795 chemical substances by application Substances 0.000 claims description 10
229920002401 polyacrylamide Polymers 0.000 claims description 10
238000002791 soaking Methods 0.000 claims description 9
239000000834 fixative Substances 0.000 claims description 8
WFKWXMTUELFFGS-UHFFFAOYSA-N tungsten Chemical compound [W] WFKWXMTUELFFGS-UHFFFAOYSA-N 0.000 claims description 7
229910052721 tungsten Inorganic materials 0.000 claims description 7
239000010937 tungsten Substances 0.000 claims description 7
GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 claims description 6
239000002253 acid Substances 0.000 claims description 6
239000007864 aqueous solution Substances 0.000 claims description 6
WSFSSNUMVMOOMR-NJFSPNSNSA-N methanone Chemical compound O=[14CH2] WSFSSNUMVMOOMR-NJFSPNSNSA-N 0.000 claims description 6
229910017604 nitric acid Inorganic materials 0.000 claims description 6
230000008569 process Effects 0.000 claims description 6
WQGWDDDVZFFDIG-UHFFFAOYSA-N pyrogallol Chemical compound OC1=CC=CC(O)=C1O WQGWDDDVZFFDIG-UHFFFAOYSA-N 0.000 claims description 6
QIGBRXMKCJKVMJ-UHFFFAOYSA-N Hydroquinone Chemical compound OC1=CC=C(O)C=C1 QIGBRXMKCJKVMJ-UHFFFAOYSA-N 0.000 claims description 5
XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims description 5
SOCTUWSJJQCPFX-UHFFFAOYSA-N dichromate(2-) Chemical compound [O-][Cr](=O)(=O)O[Cr]([O-])(=O)=O SOCTUWSJJQCPFX-UHFFFAOYSA-N 0.000 claims description 5
-1 potassium ferricyanide Chemical compound 0.000 claims description 5
YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 claims description 5
PLIKAWJENQZMHA-UHFFFAOYSA-N 4-aminophenol Chemical compound NC1=CC=C(O)C=C1 PLIKAWJENQZMHA-UHFFFAOYSA-N 0.000 claims description 4
MYMOFIZGZYHOMD-UHFFFAOYSA-N Dioxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 claims description 4
SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 claims description 4
229910001882 dioxygen Inorganic materials 0.000 claims description 4
230000005855 radiation Effects 0.000 claims description 4
CBCKQZAAMUWICA-UHFFFAOYSA-N 1,4-phenylenediamine Chemical compound NC1=CC=C(N)C=C1 CBCKQZAAMUWICA-UHFFFAOYSA-N 0.000 claims description 3
229920000936 Agarose Polymers 0.000 claims description 3
229920002301 cellulose acetate Polymers 0.000 claims description 3
239000003153 chemical reaction reagent Substances 0.000 claims description 3
238000005286 illumination Methods 0.000 claims description 3
229940079877 pyrogallol Drugs 0.000 claims description 3
ZOKXTWBITQBERF-UHFFFAOYSA-N Molybdenum Chemical compound [Mo] ZOKXTWBITQBERF-UHFFFAOYSA-N 0.000 claims description 2
229910052742 iron Inorganic materials 0.000 claims description 2
150000002736 metal compounds Chemical group 0.000 claims description 2
238000002156 mixing Methods 0.000 claims description 2
229910052750 molybdenum Inorganic materials 0.000 claims description 2
239000011733 molybdenum Substances 0.000 claims description 2
239000012286 potassium permanganate Substances 0.000 claims description 2
229910052720 vanadium Inorganic materials 0.000 claims description 2
LEONUFNNVUYDNQ-UHFFFAOYSA-N vanadium atom Chemical compound [V] LEONUFNNVUYDNQ-UHFFFAOYSA-N 0.000 claims description 2
239000002244 precipitate Substances 0.000 claims 6
238000007598 dipping method Methods 0.000 claims 3
AZQWKYJCGOJGHM-UHFFFAOYSA-N 1,4-benzoquinone Chemical compound O=C1C=CC(=O)C=C1 AZQWKYJCGOJGHM-UHFFFAOYSA-N 0.000 claims 2
KDLHZDBZIXYQEI-UHFFFAOYSA-N Palladium Chemical compound [Pd] KDLHZDBZIXYQEI-UHFFFAOYSA-N 0.000 claims 2
BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 claims 2
125000000218 acetic acid group Chemical group C(C)(=O)* 0.000 claims 1
PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims 1
229910052737 gold Inorganic materials 0.000 claims 1
239000010931 gold Substances 0.000 claims 1
125000000687 hydroquinonyl group Chemical group C1(O)=C(C=C(O)C=C1)* 0.000 claims 1
PNDPGZBMCMUPRI-UHFFFAOYSA-N iodine Chemical compound II PNDPGZBMCMUPRI-UHFFFAOYSA-N 0.000 claims 1
229910052741 iridium Inorganic materials 0.000 claims 1
GKOZUEZYRPOHIO-UHFFFAOYSA-N iridium atom Chemical compound [Ir] GKOZUEZYRPOHIO-UHFFFAOYSA-N 0.000 claims 1
229910000464 lead oxide Inorganic materials 0.000 claims 1
HTUMBQDCCIXGCV-UHFFFAOYSA-N lead oxide Chemical compound [O-2].[Pb+2] HTUMBQDCCIXGCV-UHFFFAOYSA-N 0.000 claims 1
229910000474 mercury oxide Inorganic materials 0.000 claims 1
UKWHYYKOEPRTIC-UHFFFAOYSA-N mercury(ii) oxide Chemical compound [Hg]=O UKWHYYKOEPRTIC-UHFFFAOYSA-N 0.000 claims 1
229910000487 osmium oxide Inorganic materials 0.000 claims 1
JIWAALDUIFCBLV-UHFFFAOYSA-N oxoosmium Chemical compound [Os]=O JIWAALDUIFCBLV-UHFFFAOYSA-N 0.000 claims 1
229910052763 palladium Inorganic materials 0.000 claims 1
229910052697 platinum Inorganic materials 0.000 claims 1
GGCZERPQGJTIQP-UHFFFAOYSA-N sodium;9,10-dioxoanthracene-2-sulfonic acid Chemical group [Na+].C1=CC=C2C(=O)C3=CC(S(=O)(=O)O)=CC=C3C(=O)C2=C1 GGCZERPQGJTIQP-UHFFFAOYSA-N 0.000 claims 1
239000000499 gel Substances 0.000 description 127
102000004169 proteins and genes Human genes 0.000 description 33
108090000623 proteins and genes Proteins 0.000 description 33
238000011161 development Methods 0.000 description 31
239000000975 dye Substances 0.000 description 28
238000001962 electrophoresis Methods 0.000 description 25
210000001175 cerebrospinal fluid Anatomy 0.000 description 23
CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 20
230000035945 sensitivity Effects 0.000 description 16
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 13
238000004458 analytical method Methods 0.000 description 10
229910000029 sodium carbonate Inorganic materials 0.000 description 10
238000000539 two dimensional gel electrophoresis Methods 0.000 description 10
PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 9
KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 9
238000001514 detection method Methods 0.000 description 8
239000006166 lysate Substances 0.000 description 8
QFVHZQCOUORWEI-UHFFFAOYSA-N 4-[(4-anilino-5-sulfonaphthalen-1-yl)diazenyl]-5-hydroxynaphthalene-2,7-disulfonic acid Chemical group C=12C(O)=CC(S(O)(=O)=O)=CC2=CC(S(O)(=O)=O)=CC=1N=NC(C1=CC=CC(=C11)S(O)(=O)=O)=CC=C1NC1=CC=CC=C1 QFVHZQCOUORWEI-UHFFFAOYSA-N 0.000 description 7
241000588724 Escherichia coli Species 0.000 description 7
210000001519 tissue Anatomy 0.000 description 7
230000002861 ventricular Effects 0.000 description 7
HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
210000004027 cell Anatomy 0.000 description 6
XTVVROIMIGLXTD-UHFFFAOYSA-N copper(II) nitrate Chemical compound [Cu+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O XTVVROIMIGLXTD-UHFFFAOYSA-N 0.000 description 6
239000012530 fluid Substances 0.000 description 6
238000005259 measurement Methods 0.000 description 6
230000005856 abnormality Effects 0.000 description 5
238000003556 assay Methods 0.000 description 5
230000015572 biosynthetic process Effects 0.000 description 5
238000009792 diffusion process Methods 0.000 description 5
238000002474 experimental method Methods 0.000 description 5
238000007654 immersion Methods 0.000 description 5
238000005406 washing Methods 0.000 description 5
210000004381 amniotic fluid Anatomy 0.000 description 4
238000000376 autoradiography Methods 0.000 description 4
210000001124 body fluid Anatomy 0.000 description 4
239000010839 body fluid Substances 0.000 description 4
230000008859 change Effects 0.000 description 4
238000003745 diagnosis Methods 0.000 description 4
238000004043 dyeing Methods 0.000 description 4
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238000001502 gel electrophoresis Methods 0.000 description 4
210000004698 lymphocyte Anatomy 0.000 description 4
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230000002285 radioactive effect Effects 0.000 description 4
239000012266 salt solution Substances 0.000 description 4
150000003378 silver Chemical class 0.000 description 4
PAWQVTBBRAZDMG-UHFFFAOYSA-N 2-(3-bromo-2-fluorophenyl)acetic acid Chemical compound OC(=O)CC1=CC=CC(Br)=C1F PAWQVTBBRAZDMG-UHFFFAOYSA-N 0.000 description 3
HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 3
229920001817 Agar Polymers 0.000 description 3
108010088751 Albumins Proteins 0.000 description 3
102000009027 Albumins Human genes 0.000 description 3
BPQQTUXANYXVAA-UHFFFAOYSA-N Orthosilicate Chemical compound [O-][Si]([O-])([O-])[O-] BPQQTUXANYXVAA-UHFFFAOYSA-N 0.000 description 3
238000001574 biopsy Methods 0.000 description 3
210000004369 blood Anatomy 0.000 description 3
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239000011575 calcium Substances 0.000 description 3
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239000008367 deionised water Substances 0.000 description 3
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239000012153 distilled water Substances 0.000 description 3
238000011156 evaluation Methods 0.000 description 3
230000001605 fetal effect Effects 0.000 description 3
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210000003140 lateral ventricle Anatomy 0.000 description 3
239000000276 potassium ferrocyanide Substances 0.000 description 3
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238000000926 separation method Methods 0.000 description 3
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XOGGUFAVLNCTRS-UHFFFAOYSA-N tetrapotassium;iron(2+);hexacyanide Chemical compound [K+].[K+].[K+].[K+].[Fe+2].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-] XOGGUFAVLNCTRS-UHFFFAOYSA-N 0.000 description 3
210000003462 vein Anatomy 0.000 description 3
HKJKONMZMPUGHJ-UHFFFAOYSA-N 4-amino-5-hydroxy-3-[(4-nitrophenyl)diazenyl]-6-phenyldiazenylnaphthalene-2,7-disulfonic acid Chemical compound OS(=O)(=O)C1=CC2=CC(S(O)(=O)=O)=C(N=NC=3C=CC=CC=3)C(O)=C2C(N)=C1N=NC1=CC=C([N+]([O-])=O)C=C1 HKJKONMZMPUGHJ-UHFFFAOYSA-N 0.000 description 2
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VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 2
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FJWGYAHXMCUOOM-QHOUIDNNSA-N [(2s,3r,4s,5r,6r)-2-[(2r,3r,4s,5r,6s)-4,5-dinitrooxy-2-(nitrooxymethyl)-6-[(2r,3r,4s,5r,6s)-4,5,6-trinitrooxy-2-(nitrooxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-3,5-dinitrooxy-6-(nitrooxymethyl)oxan-4-yl] nitrate Chemical compound O([C@@H]1O[C@@H]([C@H]([C@H](O[N+]([O-])=O)[C@H]1O[N+]([O-])=O)O[C@H]1[C@@H]([C@@H](O[N+]([O-])=O)[C@H](O[N+]([O-])=O)[C@@H](CO[N+]([O-])=O)O1)O[N+]([O-])=O)CO[N+](=O)[O-])[C@@H]1[C@@H](CO[N+]([O-])=O)O[C@@H](O[N+]([O-])=O)[C@H](O[N+]([O-])=O)[C@H]1O[N+]([O-])=O FJWGYAHXMCUOOM-QHOUIDNNSA-N 0.000 description 2
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AOIGFPGYIQYNPP-UHFFFAOYSA-N didodecyl sulfate;sodium Chemical compound [Na].CCCCCCCCCCCCOS(=O)(=O)OCCCCCCCCCCCC AOIGFPGYIQYNPP-UHFFFAOYSA-N 0.000 description 2
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Investigating Or Analysing Biological Materials (AREA)

JP58006552A 1982-01-18 1983-01-18 ゲル中のポリペプチドの銀染色法 Granted JPS58216958A (ja)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
US339886		1982-01-18
US06/339,886 US4405720A (en)	1981-03-04	1982-01-18	Silver stains for protein in gels

Publications (2)

Publication Number	Publication Date
JPS58216958A JPS58216958A (ja)	1983-12-16
JPH0141222B2 true JPH0141222B2 (fr)	1989-09-04

Family

ID=23331049

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
JP58006552A Granted JPS58216958A (ja)	1982-01-18	1983-01-18	ゲル中のポリペプチドの銀染色法

Country Status (1)

Country	Link
JP (1)	JPS58216958A (fr)

1983
- 1983-01-18 JP JP58006552A patent/JPS58216958A/ja active Granted

Also Published As

Publication number	Publication date
JPS58216958A (ja)	1983-12-16

Publication	Publication Date	Title
US4405720A (en)	1983-09-20	Silver stains for protein in gels
Merril et al.	1982	Simplified silver protein detection and image enhancement methods in polyacrylamide gels
Poehling et al.	1981	Visualization of proteins with a silver “stain”: A critical analysis
US4555490A (en)	1985-11-26	Rapid visualization system for gel electrophoresis
Sammons et al.	1981	Ultrasensitive silver‐based color staining of polypeptides in polyacrylamide gels
Syrový et al.	1991	Staining and quantification of proteins separated by polyacrylamide gel electrophoresis
Irie et al.	1982	A faithful double stain of proteins in the polyacrylamide gels with Coomassie blue and silver
Wijnen et al.	1996	Capillary electrophoresis of serum proteins. Reproducibility, comparison with agarose gel electrophoresis and a review of the literature
US4468466A (en)	1984-08-28	Silver stains for protein in gels--a modified procedure
Talent et al.	1998	A double stain for total and oxidized proteins from two-dimensional fingerprints
Harrington et al.	1984	Two-dimensional electrophoresis and" ultrasensitive" silver staining of cerebrospinal fluid proteins in neurological diseases.
Merril et al.	1984	" Ultrasensitive" silver stains: their use exemplified in the study of normal human cerebrospinal fluid proteins separated by two-dimensional electrophoresis.
Peats	1984	Quantitation of protein and DNA in silver-stained agarose gels
US4946794A (en)	1990-08-07	Visualization of proteins on electrophoresis gels using planar dyes
US4508820A (en)	1985-04-02	Method for simultaneously monitoring turnover rate in multiple proteins
JPH0141222B2 (fr)	1989-09-04
Grönwall	1952	On paper electrophoresis in the clinical laboratory
Hauser-Kronberger et al.	2024	Nonmicroscopical Colloidal Gold Autometallography (AMGAu): Use of Immunogold-Silver Staining in Blot Staining and Immunoassay
EP0126617B1 (fr)	1991-08-21	Détermination de macromolécules par développement physique
Rubin et al.	1983	[12] Two-dimensional polyacrylamide gel electrophoresis of membrane proteins
Vesterberg et al.	1973	Urinary proteins studied by use of isoelectric focusing. I. Tubular malfunction in association with exposure to cadmium
Kiyoko et al.	1984	An ultramicro-assay for proteins in biological fluids other than blood using a combination of agarose gel isoelectric focusing and silver staining
Patel et al.	1988	Detection of proteins in polyacrylamide gels using an ultrasensitive silver staining technique
Thaw et al.	1980	A Critical Evaluation of a Serum Seromucoid Assay and its Replacement by a Serum α1 Acid Glycoprotein Assay
Dunn	1996	Detection of proteins in polyacrylamide gels by silver staining