JPH02263153A - Electrophoretic device - Google Patents

Abstract

PURPOSE:To migrate and develop plural samples at one time under a same electrical field condition by electrically communicating the ends on respective one side of plural migrating and developing parts with a 1st common electrode chamber and electrically communicating the ends on the other side with a 2nd common electrode chamber. CONSTITUTION:Plural pieces of tubes 21, 21' for migration housing migration media are provided and buffer soln. flow passages in contact with both ends of these tubes 21, 21' for migration and ion exchange tubes 12a, 13a formed in a part thereof are provided in plural sets as well. The tubes 21, 21' for migration are so constituted that electric fields are impressed thereto by coming into contact with buffer solns. 8, 8', 9, 9' of the same or different set. The respective ion exchange tubes 12a, 13a are electrically communicated with electrolytic cells, 2, 3 which are the common electrode chambers. The electric fields are simultaneously impressed to the plural tubes 21, 21' for migration through the respectively corresponding buffer soln. flow passages. When a voltage is changed between the electrodes 6, 7.

Description

DETAILED DESCRIPTION OF THE INVENTION [Industrial Field of Application] The present invention relates to an electrophoresis device, and more particularly to an electrophoresis device including a plurality of electrophoresis developing sections each having a migration medium.

[Conventional technology]

A typical conventional electrophoresis apparatus is constructed as follows. That is, a suitable electrophoresis medium (support) such as cellulose acetate membrane, agarose gel, polyacrylamide gel, etc.
A slit-shaped hole is made near one end of the sample solution, and the sample solution is injected into the electrophoresis medium using a pipette. Electrolyte baths are connected to both ends of the electrophoresis medium through filter paper or sponge containing an electrolyte solution as a buffer. Electrophoresis is performed by placing electrodes in electrolyte baths provided at both ends of these electrophoresis media and applying a voltage so that one of these electrodes becomes positive and the other becomes negative.

[Problem to be solved by the invention]

In conventional electrophoresis devices, a single electrophoresis development section is provided within a pair of electrode chambers, and an electric field is applied to this migration development section, so that only one sample can be electrophoretically developed at a time. could not.

An object of the present invention is to provide an electrophoresis device that is capable of electrophoretically developing a plurality of samples at once under the same electric field conditions and making it possible to compare the results of electrophoresis developed by a plurality of electrophoresis developing units. It's about doing.

[Means to solve the problem]

The present invention provides a plurality of electrophoresis developing sections in association with a pair of electrode chambers, and one end of each of these electrophoretic developing sections is connected to a first electrode chamber.
electrically connected to a common electrode chamber, and the other end of each electrophoresis developing section electrically connected to a second common electrode chamber, so that an electric field can be simultaneously applied to a plurality of electrophoresis developing sections. It is characterized by what it did.

[Effect]

When a pair of common electrode chambers are provided, each common electrode chamber and a plurality of electrophoresis development sections are electrically connected, and a voltage is applied between the pair of common electrode chambers, an electric field of the same magnitude is applied to the plurality of electrophoresis development sections. Ru. When each sample from the sample introduction section connected to each electrophoresis developing section is brought into contact with each electrophoretic developing section and a voltage is applied, electrophoretic development starts for each sample at the same time. By supplying different samples in association with each electrophoresis developing section and supplying the same type of buffer solution, electrophoresis development operation with high throughput can be performed. Further, by supplying a small portion of the same sample to each electrophoresis developing section and supplying different types of buffer solutions to each electrophoretic developing section, it becomes possible to obtain development results that correspond to the components.

〔Example〕

Embodiments of the present invention will be described with reference to FIGS. 1 to 3. FIG. 3 is a diagram showing the main part configuration of an embodiment to which the present invention is applied, FIG. 1 is a diagram showing the configuration of one series of flow paths, and FIG. FIG.

In the embodiment shown in FIG. 3, there are a plurality of electrophoresis tubes 21 and 21' containing electrophoresis media, and a buffer flow path that contacts both ends of these electrophoresis tubes and an ion exchange tube formed in a part of the buffer flow path. Similarly, there are a plurality of sets of electrophoresis tubes 21 and 13a, and each of the electrophoresis tubes 21 and 21' is constructed so that an electric field is applied to it when it comes into contact with a buffer solution 8 degrees 8'9.9' of the same or different composition. ing. Each ion exchange tube is electrically connected to electrolytic cells 2 and 3 that serve as a common electrode chamber. When a voltage is applied between electrodes 6 and 7,
An electric field is simultaneously applied to the plurality of electrophoresis tubes 21, 21' through their corresponding buffer flow paths.

Next, one of the channel systems corresponding to the plurality of electrophoresis developing sections will be explained with reference to FIGS. 1 and 2. The remaining channel systems are similarly configured, and samples are supplied to each of them. In FIGS. 1 and 2, a migration medium 1 is housed in a migration tube 21 constituting one of the plurality of migration development sections 20. As shown in FIG. As the electrophoresis medium, many commonly used electrophoresis supports such as polyacrylamide gel and agarose gel can be used, but here we used polyacrylamide gel containing a buffer solution of tris-hydroxymethylaminomethane and boric acid. are doing.

A first common electrolyte tank 2 and a second common electrolyte tank 3 are provided on the left and right sides of the migration tube 21.

These electrolyte baths 2 and 3 form electrode chambers in which the electrolyte comes into contact with the electrodes. Electrolytes 4 and 5 are contained in the common electrolyte tank 2.3, and platinum electrodes 6 and 7 are partially immersed in these electrolytes. The electrodes 6 and 7 are connected to a high voltage application section (not shown), and a high voltage is applied between these electrodes.

One end and the other end of the electrophoresis tube 21 are connected to respective tube members 22 and 23 constituting a buffer flow path, as shown in FIG. 2. The liquid feeding tube 12.13 and the ion exchange tubes 12a, 13a are connected to the corresponding tube member 22.2.
3 to form a buffer flow path. Buffer solutions 8.9 each consisting of an electrolyte-containing solution are housed in replaceable containers.

The buffer solutions 8 and 9 are each sent through the liquid sending tubes 12.13 by pumps 10.11, and are discharged from the drain ports 14 and 15, respectively, in contact with the ends of the electrophoresis development section 20. There is. Liquid feeding tube 12.13 and ion exchange tube 12a.

The buffer solution flow paths having 13a are arranged to pass through the electrolyte solution tanks 2 and 3, respectively, and at least a portion of the portion of each buffer solution flow path in the corresponding electrolyte solution tanks 2 and 3 is Each ion exchange tube (e.g. DuPont Na
tion tube) 12a, 13a.

The ion exchange tubes 12a, 13a are connected to the electrolytes 4, 5 in the common electrolyte baths 2, 3 in which the electrodes 6°7 are arranged,
It functions to prevent free flow of the buffer solution flowing in the buffer solution channel. However, the buffer solutions 8, 9 in the buffer solution flow path and the electrolytes 4, 5 in the common electrolyte tanks 2, 3 are electrically connected via ion exchange tubes 12a, 13a, respectively.

By providing an isolation part such as an ion exchange tube that can electrically communicate both liquids, when a voltage is applied between the electrodes 6 and 7, the electrolytes 4 and 5, the ion exchange tube 12a,
13a and the buffer solutions 8 and 9 in the buffer flow path, a voltage is applied to both ends of the electrophoresis developing section 20.

Next, a method for adding a sample will be explained. A sample injection part 24, 25, such as a sampling valve with a sample measuring tube used in a liquid chromatograph, etc., is connected to a liquid sending pump 10.
．． 11 and the common liquid tanks 2, 3, the liquid feeding tube 12,1.
3, and a detector is installed in the flow path on the drain port 14.15 side. The sample injection section 24, 25 and the detector may be provided in only one of the two buffer flow paths, or may be provided in both. When a sample is introduced into each buffer channel from one of the sample injection sections 24 or 25, the sample band flows through the buffer channel while being sandwiched by the buffer solution and is carried toward the migration development section 20.

As shown in FIG. 2, when the sample band is moved so as to come into contact with one end of the electrophoresis developing section 20, the liquid feeding operation of the liquid feeding pump 10 or 11 is stopped, and the flow of the buffer solution is stopped. In this state, a constant electric field is applied to the entire electrophoresis development area using electrodes 6 and 7. Each sample 16 that was in contact with the end of the electrophoresis development section
A part of the sample component is taken into each corresponding electrophoretic development section 20 by applying an electric field, and the sample components start electrophoretic development within the electrophoresis medium 1. After a certain period of time has passed since the application of the constant electric field, the liquid feeding operation of the liquid feeding pump 10 or 11 is restarted, and the remaining sample 16 is transferred by the buffer solution and discharged from the liquid drain port 14 or 15. By supplying the buffer solution in this way, one end of the electrophoresis developing section 20 is cleaned, so that preparations are made for introducing the next sample. Such a method facilitates sampling automation.

〔Effect of the invention〕

According to the present invention, it is possible to perform electrophoresis separation at the same time in a plurality of electrophoresis development sections, and it is possible to perform multicomponent separation in which the same sample is performed in multiple electrophoresis development sections, or to perform simultaneous electrophoresis development sections for multiple different samples in an efficient manner. Electrophoretic separation becomes possible.

[Brief explanation of drawings]

FIG. 1 is a schematic configuration diagram for explaining one series in one embodiment of the present invention shown in FIG. FIG. 3 is a longitudinal cross-sectional view showing the configuration of essential parts of an electrophoresis device that is an embodiment of the present invention.

Claims (1)

[Claims] 1. In an electrophoresis apparatus that performs electrophoretic development of a sample by applying an electric field to a migration development section having a migration medium, a plurality of migration development sections are provided, and one end of each of the plurality of migration development sections is connected to the electrophoresis device. electrically connected to one common electrode chamber, the other end of each of the plurality of electrophoresis developing sections electrically connected to a second common electrode chamber, and applying an electric field to the plurality of electrophoresis developing sections simultaneously. An electrophoresis device characterized in that it is configured to allow