JPH0249710A - Cosmetic - Google Patents

Abstract

PURPOSE:To obtain a cosmetic having excellently beautifying effects on skin, wetting effects on skin, activating effects on cells and antioxidation effects by blending a cosmetic composition with one or more extracts extracted from each fruit body of specific six kinds of fruit bodies with water or/and an organic solvent. CONSTITUTION:A cosmetic composition is blended with preferably 0.5-10wt.% based on total amount of cosmetic composition of one or more extracts extracted from Polyporus confluens Pr., Rozites caperata, Lyophyllum shimeji, Russula lilacea Quel, Ramaria fumigata and Grifola frondosa with water or/and an organic solvent as an active ingredient to give a cosmetic having beautifying effects on skin resulting from inhibitory action on tyrosinase activity, wetting effects on skin resulting from moisture retaining effects on skin of viscous substance, beautifying effects on skin and synergistic effects of prevention and removal of wrinkles of skin resulting from activating action on cells, preventing effects on change of quality of cosmetic and improving effects on stability of cosmetic resulting from antioxidation effects and extremely low irritation and toxicity.

Description

DETAILED DESCRIPTION OF THE INVENTION <Industrial Application Field> The present invention relates to a novel cosmetic, particularly a cosmetic having an excellent skin whitening effect or a skin whitening effect.

The present invention relates to a cosmetic having one or more effects selected from among the following effects: skin moisturizing effect, cell activation effect, and antioxidant effect. More specifically, various substances contained in the extract extracted from the various mushrooms (fruiting bodies) with water and/or organic solvents, that is, (a) in addition to substances having a skin whitening effect based on the tyrosinase activity inhibiting effect; ,(
b) Substances that have a skin moisturizing effect based on skin moisturizing effect (
Viscous substance) (C) A substance that has a skin whitening effect based on cell activation and a synergistic effect of preventing and removing skin wrinkles, (
d) It relates to cosmetics that contain substances as active ingredients that have the effect of preventing deterioration and improving the stability of cosmetics based on antioxidant effects, and which have extremely low irritation and toxicity.

<Prior Art and Background of the Invention> It is believed that the pigment melanin is deeply involved in the darkening of the skin. That is, it is thought that melanin is produced in the skin tissues of the skin in response to external stimuli such as ultraviolet rays, which promotes the darkening of the skin and causes symptoms such as age spots, freckles, and dark skin.

There are several mechanisms of action for skin whitening, one of which is proposed to be inhibition of the activation of tyrosinase (enzyme) involved in melanin production, and other mechanisms of action for skin whitening have been proposed. By activating the metabolism of skin cells, it promotes the turnover of melanin pigments deposited on the skin, thereby eliminating symptoms such as age spots, freckles, and dark skin.

Conventionally, glutathione has been considered to be effective as a known substance capable of suppressing the activity of tyrosinase. However, when cosmetics containing glutathione are moist, glutathione is easily oxidized and easily causes discoloration and odor, which poses a fatal problem, especially for cosmetics. Furthermore, it is known that there are substances in crude drugs (natural products) that have the effect of inhibiting tyrosinase activity. All of the known tyrosinase activity inhibitors present in these natural products have the advantage of being less irritating, but on the other hand, they have the problem of a low tyrosinase activity inhibition rate. Therefore, it is a substance that exists in natural products and has a high inhibition rate of tyrosinase activity.
Moreover, there is a desire to discover and develop chemically stable, preservative, and safe substances suitable for cosmetics.

In addition, conventional products derived from natural products that have whitening effects include Kafu, Hamakaze, and Tsukuritake (Agaricus).
It has been reported that the extract of P. bisporous contains a substance that has a skin-whitening effect. (Special Publication No. 59-48804) On the other hand, research on discovering and searching for substances contained in natural products that activate cell activating effects in skin, etc. is an undeveloped field, and especially natural products that are safe and stable. Alternatively, it is desired to search for cell activating substances derived from natural products.

By the way, from the above-mentioned safety perspective, it is essential for cosmetics to prevent deterioration and ensure stability, and oxidation prevention is a typical measure to prevent deterioration of cosmetics. Can be mentioned. Among the antioxidants that can be used in cosmetics, chemically synthesized substances have many side effects and safety issues, so it is desirable to use natural products or substances derived from natural products as much as possible. ing. Ascorbic acid and tocopherol are known as typical antioxidants conventionally used in cosmetics.

However, while ascorbic acid is easily soluble in water, it is unstable in a water system. On the other hand, tocopherols have problems such as being difficult to dissolve in water, which limits the range of cosmetics that can be used for them. Therefore, it is desired to develop an antioxidant that is a natural product or a substance derived from a natural product, is non-irritating, safe, easily dissolves in water, and is stable even in an aqueous system.

Problems to be Solved> The present invention aims to solve the above-mentioned problems and meet the above-mentioned demands.

That is, the inventors searched for and developed new substances that are natural products or substances derived from natural products, are safe and stable with little irritation, and are useful as additives for cosmetics. Among various natural products, especially various mushrooms (fruiting bodies)
was selected as a raw material, and various extracts of various mushrooms (fruiting bodies) with water and/or organic solvents were searched for the presence or absence of tyrosinase activity inhibitors, measurement of tyrosinase activity inhibition rate, and presence or absence of skin moisturizing substances (viscous substances). search, cell proliferation effect, antioxidant effect, etc.

We searched for various new substances that have properties useful as additives for cosmetics, and conducted various tests to measure their cell proliferation effect (cell activation effect) and antioxidant effect (potency). As a result, ■Ningyotake [Polypor
us confluencesPr, ], Shogenji [Rozites caperata (Pers
, ex Pr,) Karst,), Honshimeji [Lyophyllum shimeji (
Kawam,) Hong.

], Usumurasakihara Ru5sula 1il
[:R
amaria fumigata K, Yokoyam
a ] and maitake [Grifola fron
dosa (Dicks, ax Fr.

) Extracted from each fruiting body of S, F, and Gray'll (fruiting bodies of each of the six strains mentioned above) with a single solvent selected from the solvent group consisting of organic solvents, glycols, and water, or a mixed solvent of multiple thereof. In particular, the mushroom extract of each of the six strains of the present invention contains a substance that has a skin whitening effect based on its excellent tyrosinase activity inhibiting effect.
Extracts extracted from fruiting bodies) with water alone, or extracts extracted with a mixture of water and an organic solvent,
Regarding any of these extracts, in addition to the skin whitening effect based on the above-mentioned tyrosinase activity inhibiting effect, it also contains a new substance (viscous substance) that has a skin moisturizing effect based on the excellent skin moisturizing effect. Regarding extracts extracted from the substance with a single solvent selected from the group of organic solvents, glycols, and water solvents, or a mixed solvent of multiple of these solvents, in addition to the various effects mentioned above, there is a novel compound that has a remarkable antioxidant effect. (2) In particular, regarding the aqueous extract of the fruiting body of N. gyotake (Aqueous extract of N. g. , etc. were discovered, and this invention was completed based on the above discoveries.

This invention provides a cosmetic containing one or more extracts selected from the group of extracts extracted from each of the above-mentioned seed bodies (all six types of fruiting bodies) with water and/or an organic solvent according to the present invention. In terms of cosmetics, the objective is to provide cosmetics that are highly safe and stable, have little irritation, and have a skin whitening effect based on an excellent tyrosinase activity inhibiting effect, and a water extract extracted with water alone. Regarding cosmetics containing various extracts extracted with a mixture of organic solvents or water and organic solvents, cosmetics that have both the skin whitening effect and the excellent skin moisturizing effect based on the moisturizing effect of viscous substances are used. The primary purpose is to provide.

In addition, the present invention provides an extract extracted from N. chinensis using a single organic solvent or a mixture of a plurality of organic solvents, and an extract extracted from N. chinensis with a mixture of one or more organic solvents and water. Regarding cosmetics containing one or more extracts selected from various extract groups, in addition to the above-mentioned tyrosinase activity suppressing effect, or both the above-mentioned tyrosinase activity suppressing effect and skin moisturizing effect, it has an antioxidant effect. The second objective is to provide a cosmetic with improved stability and safety.

In particular, cosmetics containing the aqueous extract of Ningyotake extracted from Ningyotake with water alone have a skin whitening effect based on the above-mentioned tyrosinase activity inhibiting effect, a skin moisturizing effect due to the viscous substance, and an antioxidant effect. In addition to the effect of improving the stability and safety of cosmetics, it has a cell activating effect, resulting in a synergistic effect of skin whitening effect and skin wrinkle prevention and removal effect based on the above-mentioned tyrosinase activity suppressing effect and cell activating effect. The third objective is to provide a smooth cosmetic that has both the effect of improving the stability and safety of cosmetics based on the antioxidant effect of the water extract of Ningyotake mushroom, and the skin moisturizing effect based on a viscous substance. This is the purpose.

<Means for Solving the Problems> In order to achieve the above object, the present invention uses Polyporus confluences Fr.
] (hereinafter simply referred to as "Rozites cape"), Rozites cape of the Fusentake family.
rata (Pers, exFr,) Karst
, ] (hereinafter simply referred to as "Shogenji"), Lyophyllum shimeji (Kaw.
am, ) Hongo ] (hereinafter simply referred to as "Hon-shimeji"), Ru5s
ula lilacea Qugl] (hereinafter simply referred to as "Usmurasakihara"), Ramaria fumig of the Ramaria family.
ata K, Yokoyama] (hereinafter simply referred to as "Usmurasakiboukitake") and Maitake [Grifolafrondos] of the Monkey family.
a (n1cks, ex Fr, ) S, F,
Gray] (hereinafter simply referred to as "Maitake") A cosmetic characterized by containing one or more extracts selected from the group of extracts extracted from each fruiting body of Maitake mushrooms with water and/or an organic solvent. It was decided that this would constitute a fee.

First, we will explain in more detail the method for preparing various extracts from the six types of mushrooms (fruiting bodies).As raw materials for extraction, fresh or dried mushrooms (fruiting bodies) can be used. , drying the fruiting body in the sun for about 2 to 5 days,
It is preferable to use one that has been prevented from rotting or the like.

Then, appropriate amounts of the various mushrooms (fruiting bodies) used in the present invention are taken out and various effective substances are extracted as extracts using various solvents described below. As the solvent used to extract various effective substances from each of the seed bodies, a single solution selected from the group consisting of various organic solvents and water, or a mixture of a plurality of these solvents can be used.

Examples of the organic solvents used in the present invention to extract various effective substances from the seed bodies include methanol, ethanol, propatool isopropyl alcohol, n-butanol, lauryl alcohol, cetanol, stearyl alcohol, and oleyl alcohol. A single solvent of one or both of linear monohydric alcohols having 1 to 22 carbon atoms such as alcohol and behenyl alcohol and side chain monohydric alcohols and/or a mixture of a plurality of solvents can be used. In addition, various ketones such as acetone, ethyl methyl ketone, and isopropyl methyl ketone, various esters such as ethyl acetate and ethyl ester of fatty acids, halogenated alkanes (mainly chlorides) such as dichloromethane, chloroform, and dichloroethane, diethyl ether, Various ethers such as ethyl cellosolve, other aromatic hydrocarbons such as benzene and toluene, etc., various polar organic solvents and various non-polar organic solvents, one or both of which are used alone and/or in a mixture of multiple solvents are used. Available. Further, the above-mentioned various organic solvents can be used alone, or a mixed solution of a plurality of various organic solvents mixed at an appropriate blending ratio can also be used. Of course, it is also possible to use a heated molten one or a mixed molten one of two or more kinds of solvents, etc., as needed. Lower alcohols with 1 to 5 carbon atoms such as tool and lobetanol, ethers such as diethyl ether, ketones such as acetone, ethyl methyl ketone, and isopropyl methyl ketone, esters such as ethyl acetate, and halogenated alkanes such as dichloromethane , chloroform, dichloroethane, and aromatic hydrocarbons, organic solvents with high to medium polarity such as benzene are particularly suitable.

Examples of the glycols used for extracting various effective substances from each seed body according to the present invention include:
Polyethylene glycol, propylene glycol, 1
．． 3-butylene glycol (hereinafter simply referred to as r1.3-BG)
Various glycols such as J) can be used. In addition, polyhydric alcohols such as glycerin, diglycerin, and triglycerin can be used. Among the glycols,
Among them, 1,3-butylene glycol (1,3-BG)
is particularly suitable.

Further, as the solvent used to extract the effective substance from each of the seed bodies according to the present invention, water alone or a mixture of water and the organic solvent (mixture of water and organic solvent) may be used. be able to.

Furthermore, in order to extract the effective substance from each of the seed bodies according to the present invention, a single solution of various solvents selected from the solvent group consisting of the various organic solvents, glycols, and water can be used; A mixture of two or more of these solvents (for example, 30-50% aqueous ethanol, benzene:ethyl acetate (4:1 mixture), 1,3-butylene glycol aqueous solution, etc.) can be used, and furthermore, Of course, a mixture of one or more of the organic solvents and one or more of the glycols, or a mixture of these various organic solvents, glycols, and water can be used.

By the way, among the various extraction solvents that can be used to extract various effective substances from each seed entity according to the present invention, "
As mentioned above, a mixture of water and an organic solvent can be used. The "mixture of water and organic solvent" (hereinafter referred to as "water/organic solvent mixture") referred to here means 1
It refers to a mixed liquid in which water is actually contained or mixed in a certain proportion (compounding ratio) in a species or two or more organic solvents. Furthermore, the organic solvent referred to herein basically refers to the various organic solvents that can be used to extract various effective substances from the seed bodies as described in detail above. In reality, as the organic solvent used in the water/organic solvent mixture, it is best to use an organic solvent that is easily soluble in water or an organic solvent that can dissolve water to some extent.

When "water alone" or the above-mentioned "water/organic solvent mixture" is used as a suitable extraction solvent, (a) skin whitening based on the tyrosinase activity inhibiting effect from each seed body (six types) according to the present invention; or (b) from one of the six seed bodies mentioned above, This is a case of extracting a group of effective substances that have three effects: tyrosinase activity suppressing effect, skin moisturizing effect, and antioxidant effect. In addition, in particular, when only "water alone" is used, (C) a group of effective substances that have a cell activating effect in addition to the tyrosinase activity inhibiting effect, skin moisturizing effect, and antioxidant effect are extracted from Nymph mushroom. This is the case.

Next, specific extraction operations for extracting various effective substances and/or groups of effective substances from each of the seed bodies using the various extraction solvents (water and/or organic solvents) will be described.

An extraction solvent such as water or ethanol (1 to 100 parts by weight or 1 to 100 parts by weight) is added to each of the dried (including air-dried and sun-dried) or fresh seed bodies (1 part by weight or 1 part by volume). After adding 100 parts by volume, preferably 1 to 50 parts by weight or 1 to 50 parts by volume, extraction is performed once to three times on a boiling water bath and under reflux heating conditions for about 1 hour each time, followed by solid-liquid separation ( centrifugation, filtration, etc.) to obtain an extract (in the above example, an aqueous extract or an ethanol extract). This extract is prepared by distilling off the solvent, measuring the yield of various extracts such as viscous substances containing fluorescein, storing it, diluting it to an appropriate concentration if necessary, and then inhibiting the tyrosinase activity below. It is used as a sample for measurement of rate, cell activation effect, antioxidant effect (potency), etc.

In addition to the above-mentioned examples of water and ethanol, the extraction solvent may include a single solution of various desired organic solvents (e.g., glycol, dichloromethane, etc.) or a mixture of two or more selected from the solvent group consisting of various organic solvents and water. A liquid can be used, and an extract of each of the seed bodies can be similarly obtained by the above-mentioned extraction operation. Each extract of each seed body thus obtained is used as a sample for the various measurements described above, such as the measurement of the inhibition rate of tyrosinase activity.

Each of the above-mentioned extracts (e.g., ethanol extract, dichloromethane extract, distilled water extract, etc.) of each seed body prepared in this way is used for various purposes such as measurement of tyrosinase activity inhibition rate, etc. used to measure the characteristics of As a result, each extract of each of the seed bodies had the following characteristics.

(2) A remarkable tyrosinase activity inhibiting effect is observed in each of the extracts extracted from the fruiting bodies of the six types of bacterial strains according to the present invention with water and/or an organic solvent.

■The extract extracted from each fruiting body of the six types of bacterial strains according to the present invention with water or a water/organic solvent mixture has an excellent skin moisturizing effect in addition to the remarkable tyrosinase activity inhibiting effect described in (■) above. The presence of a viscous substance is observed.

■ A single organic solvent solution, a mixed solution of multiple organic solvents, a mixed solution of a single organic solvent and water, or a mixed solution of multiple organic solvents and water from N. chinensis, which is one of the six types of strains according to the present invention. The extract extracted with the mixed solution has the above-mentioned tyrosinase activity inhibiting effect, or the above-mentioned tyrosinase activity inhibiting effect and skin moisturizing effect.

In addition, significant antioxidant effects are observed. Moreover, this “
"Organic solvent extract of Ningyotake mushroom" and "Water/organic solvent mixture extract of Ningyotake mushroom" both have the excellent property of being well soluble in water.

■ The aqueous extract of N. gyotake, which is one of the six types of strains according to the present invention, is extracted with water alone, and has the effect of inhibiting the tyrosinase activity described in (2) above, and the skin moisturizing effect of the viscous substance described in (2). , In addition to the antioxidant effects described in (1) above, a remarkable cell activation effect is observed.

The inhibition rate of the tyrosinase activity of each extract of each seed body is measured by the following method. That is, each extract of each of the seed bodies may be used as needed, e.g.
%1.3-butylene glycol aqueous solution (hereinafter simply "30
% 1.3-Bo solution (hereinafter referred to as "bo solution"), etc., to an appropriate concentration (for example, about 0.1 to 10%, etc., in a range where the absorbance at 475 nm shows an appropriate value). The diluted solution of each extract of each of the seed bodies of Chinoku chinensis (concentration) was used as the "test solution for measurement".

Add one tyrosine solution (concentration = 0.3 mg) to each test tube.
/all 1ml and pine kilvain buffer (Mc
llvain Buffer 5 solution
) (pH 6,8) 1mf, and in each of these test tubes were added the diluted solution of each extract of each seed body (the "test solution for measurement") and 30% 1.3 for blank test. - Add 0.9 ml of each Bo solution and incubate for 10 minutes in a constant temperature water bath at 37°C.

Add 0.1- of tyrosinase solution (concentration: 1 mg/-・pine kilvain buffer) to the above-incubated material.
In addition, after stirring thoroughly, each reaction solution was immediately set in a spectrophotometer and the absorbance at 475 nm was measured over time. (
As the absorbance value at each measurement time point, the absorbance value immediately after the addition of the tyrosinase solution is indicated with a suffix ◎, and the absorbance value after X minutes of incubation after addition is indicated with the suffix X.) Each absorbance value The tyrosinase activity inhibition rate is calculated by substituting 0 into the following 0 formula. In addition, in calculating the tyrosinase activity inhibition rate of this invention, the absorbance value 10 minutes after the addition of the reaction solution is used.

Tyrosinase activity inhibition rate Bo Absorbance value of Ni blank solution after 0 minutes Bx Absorbance value of Ni blank solution at X minute diameter Ao : Absorbance value of test solution after 0 minutes Ax : Absorbance value of test solution at X minute diameter The value is dopachrome (
It is measured by the amount of melanin (precursor of melanin) produced.

As is clear from the extraction process, the extracts extracted from each seed body of the raw material according to the present invention with water and/or an organic solvent can be extracted using various organic solvents (polar solvents,
Excellent solubility in non-polar solvents), glycols, water, etc. In particular, it has excellent solubility and miscibility in various bases and various organic solvents used in cosmetics. In other words, organic solvents commonly used in cosmetics include ethanol, isopropyl alcohol, lauryl alcohol, cetanol, stearyl alcohol, oleyl alcohol, behenyl alcohol, propylene glycol, 1,3-butylene glycol, dipropylene glycol, isopropyl myristate, palmitin. It has excellent solubility in isopropyl acid, various other animal oils, vegetable oils, etc.

The above-mentioned extracts extracted from the seed bodies using the above-mentioned solvents and methods can be blended into various known cosmetic bases, etc. to create creams, milky lotions, lotions, packs, etc.
It can be widely applied to various basic cosmetics such as facial cleansers, foundations, various makeup products such as white powder, soaps, shampoos, conditioners, perfumes, colognes, and other cosmetics.

In addition, the shapes of the various cosmetics include solutions, emulsions,
ointment, oil, wax, gel, sol.

It can be applied in various forms such as powder (powder) and spray (aerosol).

Similarly, the extracts extracted from the seed bodies using the solvents and extraction methods described above are non-toxic and non-irritating to the skin (based on patch tests, etc.), and are highly stable against heat and light. In addition, various cosmetic bases, various cosmetic additives (for example, various surfactants,
Solvent 2 dye. It has outstanding properties such as high affinity and stability for fragrances, preservatives, antioxidants, humectants, vitamins, animal and plant extracts, and other various additives, and is used as a base for various cosmetics and cosmetics. It can also be used in combination with additives.

The amount of the extract extracted from each of the seed bodies contained in various cosmetics is not particularly limited, as it can be selected and varied depending on the form of use. In principle,
It is sufficient if it is present in an effective amount, but - for boat fishing, it should be present in an amount of 0.01 to 20% by weight (w) based on the total weight of the cosmetic composition.
t%), preferably about 0.5 to 10% by weight (wt%).

(1) The cosmetic according to the present invention is made from the raw materials used in the present invention, that is, from each of the six types of mushrooms (fruiting bodies): Prunus chinensis, Shougenji, Honshimeji, Usmurasakiha, Usmurasakiboukitake, and Maitake. Cosmetics that contain one or more extracts selected from the extract groups extracted with various organic solvents, glycols, and/or water must have the tyrosinase activity-inhibiting effect of the extracts involved in the formulation. Based on this, it exhibits excellent skin whitening effects.

Furthermore, the cosmetic according to the present invention contains one or more extracts selected from the group of extracts extracted from each of the six types of fruiting bodies with water or a water/organic solvent mixture. In addition to the skin whitening effect based on the properties of the aqueous extract and water/organic solvent mixture extract, that is, the tyrosinase activity inhibiting effect, the cosmetic contains a viscous substance, so the viscous substance has a skin moisturizing effect. The skin moisturizing effect based on the skin moisturizing effect also has the remarkable effect of imparting a moist feel and smoothness to the skin, thereby exhibiting a synergistic effect between the skin moisturizing effect and the skin whitening effect.

(2) Contains one or more extracts selected from the group of extracts extracted from Ningyotake, which is one of the raw materials according to this invention, with an organic solvent or a water/organic solvent mixture. In addition to the skin whitening effect, skin moisturizing effect, etc. mentioned in (1) above, cosmetics can also be used based on the properties of the organic solvent extract of the Ningyotake mushroom or the water/organic solvent mixture extract of the Ningyotake mushroom, that is, the antioxidant effect. It can prevent deterioration of cosmetics, improve the stability and safety of cosmetics, and exhibit a synergistic effect of skin whitening or skin whitening/skin moisturizing effects as well as antioxidant effects.

(3) A cosmetic containing a water extract of Nymph mushroom, which is one of the raw materials according to the present invention, has the skin whitening effect and skin moisturizing effect as described in (1) above.

In addition to the antioxidant effect described in (2) above, based on the properties of the water extract of Prunus japonica, that is, the cell activating effect and the antioxidant effect, the synergistic effect of the tyrosinase activity suppressing effect and the cell activating effect makes it even more outstanding. It has a skin whitening effect and a skin wrinkle prevention/removal effect due to its cell activation effect, and it also provides a smooth and moist feeling to the skin, and has a remarkable anti-deterioration effect and cosmetic effect based on its antioxidant effect. It is possible to improve the stability and safety of the food.

<Example> Next, the results of the tyrosinase activity inhibitory effect test and the content of viscous substances of various extracts extracted from each of the seed bodies with water and/or organic solvent, Example results of tests to measure the antioxidant effect (potency) and the cell activation effect of the water extract of Ningyotake, as well as examples of application formulations of each extract of each seed body according to the present invention to cosmetics, etc. However, it goes without saying that the invention is not limited to the embodiments described herein.

■] Test results for the inhibitory effect on tyrosinase activity of extracts extracted from each seed body with water and/or organic solvent, and investigation status of viscous substances.

(1) Fruiting bodies as raw materials: Each of the above-mentioned seed bodies according to the present invention, that is, Ningyoutake, Shougenji, Honshimeji, and Usmurasaki Yatsu. Both Boukitake mushrooms and Maitake mushrooms are widely used as food and are known to have extremely low toxicity. Many of these fruiting bodies were collected from plants that were growing naturally in nature. In other words, Ningyoutake, Honshimeji and Usmurasakihokitake are common in Yamagata District, Hiroshima Prefecture, and Shogenji,
and Usmurasakihara were collected in Ina City, Nagano Prefecture. In addition, commercially available maitake mushrooms are used.

(2) Pre-treatment of raw materials: Fruiting bodies other than dried maitake mushrooms are collected from the natural world.These fruiting bodies collected from the natural world are dried in the sun for about 3 days mainly from the viewpoint of improving preservation. Use the one you made. Then, the various dried fruit bodies (all six types of fruit bodies) according to the present invention are crushed and used for extract preparation. (3) Preparation of various extracts from the fruit bodies: 6 types) Extraction solvent 5 for 1g of each
0- is added and heated under reflux for about 1 hour for extraction on a boiling water bath.

After cooling, a first solid-liquid separation is performed by centrifugation (3,00 rpm, 10 minutes) or the like. A supernatant liquid is taken, and this supernatant liquid is further filtered using a filter paper to obtain a first extract liquid. on the other hand,
The residue after the first solid-liquid separation was subjected to operations such as reflux heating extraction for about 1 hour on a boiling water bath and solid-liquid separation/filtration twice using the extraction solvent 25- as in the previous time. All of the extracts (filtrates) from each round are combined and the various extraction solvents are distilled off under reduced pressure to obtain extracts from each seed body with the various solvents. In this example, Table 1 shows examples of results when water, 100% ethanol, and dichloromethane were used as the extraction solvents.

(4) Preparation of test solution of each seed body extract for tyrosinase activity inhibition effect measurement test: Add 30% to each extract of each seed body prepared in (3) above.
1.3-BG solution is added, heated under reflux on a boiling water bath for about 30 minutes, and then cooled. After cooling, it is filtered, and the filtrate is subjected to a test to measure the inhibitory effect on tyrosinase activity. In addition,
When conducting a tyrosinase activity inhibition test, 475n
m1. The concentration of the extract was adjusted to 30% in order to adjust the absorbance value at 1.3-B to a value suitable for measurement.
It is important to dilute and adjust the concentration to an appropriate level using G solution.

(5) Experiment for measuring the tyrosinase activity inhibition rate of each extract of each of the seed bodies according to the present invention: The tyrosinase activity inhibiting effect of each of the extracts of each of the seed bodies thus obtained was determined by the method described above. Measure. Each extract was added to a 30% 1.3-BG solution at a given concentration (
When measuring the inhibitory effect on tyrosinase activity, it is diluted to a concentration suitable for showing an appropriate absorbance value at 475 nm and used for measurement.

To measure the tyrosinase activity inhibition rate of each extract of each seed body, each absorbance was measured after 10 minutes, and the tyrosinase activity inhibition rate (%) of each extract was calculated using the above formula 0. The results of the tyrosinase activity inhibition rate of each extract are shown in Table 1.

Tyrosinase activity inhibition rate Bo Absorbance value of blank solution after 0 minutes Boo
Absorbance value Ao of the blank solution after 10 minutes: Absorbance value Ago of the test solution after 0 minutes: 1 of the test solution
Absorbance value after 0 minutes (6) Preparation method of viscous substance from each seed body: 1 g of each seed body according to the present invention (
Add 50 ml of ethyl acetate (dry weight) and heat to reflux on a boiling water bath for about 2 hours. After cooling, ethyl acetate is filtered off and solid-liquid separation is performed. Then, the residue on the filter paper is transferred to a flask with 50-g of ethanol, heated under reflux for about 1 hour on a boiling water bath, and then the ethanol is filtered off. In this way, the oily substance in the fruiting body is extracted and removed into the ethyl acetate layer and the ethanol layer. Furthermore, the residue on the filter paper is transferred to a flask, and the procedure of refluxing, heating and extracting in a boiling water bath for about 1 hour, followed by filtering is repeated twice to extract the viscous substances from each seed body. The above-mentioned viscous substance extracts and washing liquids were combined, concentrated to about 20 mL, 80 ml of ethanol was added, stirred well, the precipitate was collected, washed twice with a small amount of ethanol, and then vacuumed. Dry at 50-60°C for 1 hour) and determine the dry weight of the viscous substance extracted from the fruiting body. In addition, among the seed bodies (six types) according to the present invention, Ningyotake contains a particularly large amount (approximately 6% of the dry weight) of the viscous substance,
It has been found that in the test fruiting bodies other than Ningyotake, the viscous substance is contained in an amount of about 0.1 to several percent based on the dry fruiting body.

Table 1 shows (a) the yield of each extract (dry weight) relative to the dry fruiting body (unit double % = wt% or less) of each extract of each seed body according to the present invention; Example of results in which the concentration (wt%) of the extract at the time of the tyrosinase activity inhibition test measurement of each extract, and (C) the tyrosinase activity inhibition rate (%) at the concentration measured in the tyrosinase activity inhibition test on each extraction solvent side. and a table showing a comparative example of the content of viscous substances.

The results in Table 1 suggest the following.

■Each extract extracted from the above-mentioned seed bodies (all six types) according to the present invention with water and/or an organic solvent,
It has an excellent tyrosinase activity inhibitory effect. Each of the extracts extracted from the above-mentioned seed bodies (all 6 types) with water or a water/organic solvent mixture contains a viscous substance that has a skin moisturizing effect.

■When water is used as an extraction solvent from each of the seed bodies (all six types) according to the present invention, a large amount of the extract itself is extracted compared to other methods, and in terms of the yield of the extract. The order is Honshimeji mushroom, Murasakihara mushroom, Maitake mushroom, Ningyotake mushroom, Shougenji mushroom, and Usmurasakiboukitake mushroom, and about 50% to 29% of the dry weight of the fruiting body is extracted.

■When ethanol is used as an extraction solvent from the above-mentioned seed bodies (all 6 types) according to this invention, the yield of the extract decreases slightly, but the ethanol extract exhibits excellent tyrosinase activity inhibition at low concentrations. showing the rate.

etc. are suggested in Table 1.

In addition, the inventors used aqueous ethanol (for example, 30%
It has been found that even when an aqueous solution of ~50% ethanol, etc.) is used as an extraction solvent, the yield of each extract and the inhibitory effect on tyrosinase activity can be shown to be equivalent to or higher than that of water or 100% ethanol.

(Margins below this page) ■] Example of a measurement test for the cell activation effect of a water extract of N. japonica.

The cell activating effect of the water extract of Ningyotake is tested by measuring the cell proliferation effect of the extract.

(1) Preparation of aqueous extract of Ningyotake: After washing the fruiting body (dried product) Ig of Ningyotake three times with 30 μm of methanol, add 100 μl of water, and perform the reflux heating extraction operation on a boiling water bath for about 1 hour. Repeat times. After extraction, the aqueous extract is combined and the water is distilled off or evaporated to dryness to obtain 0.42 g of dry matter of the aqueous extract of N. chinensis.

Then, phosphate buffer aqueous solution (NaC1:8.Og, K
CI: 0.2g, NaJPO4: 1.15g, KHa
POn: 0.2g was dissolved in distilled water to make 11. Prepare pH 7.4).

On the other hand, a Ningyotake sample solution is prepared by dissolving the Ningyotake aqueous extract prepared as described above in the phosphate buffer aqueous solution. The concentration of the N. gyotake sample solution for preparation was 0% (blank 9 without addition: control) 10%, 2
Measurements were compared by setting six concentrations: 0%, 30%, 40%, and 50%.

(2) Preparation of cultured cells: Fibroblasts induced from the back skin of guinea pigs by a conventional method are used as cultured cells.

(3) Cell culture method: Eagle's MEM medium containing 2% tallow serum is used as the basic medium. 4 or 5 cuts containing 1rnl of the above basal medium
8. Place the cultured cells described in (2) above in a plastic petri dish. OXIO' cells are sown, and 1-1 of the N. japonica sample solution described in (1) above is added, followed by culturing for 6 days at 37 tons under 5% carbon dioxide gas conditions.

(4) Measurement of proliferation of cultured cells: The cell proliferation effect (cell activation effect) is measured by measuring the proliferation of cells after culture.

As a specific method, after culturing the cells described in (3) above, the cells are fixed with a 10% formalin solution, and then fixed with 0.05% naphthol blue black (9% acetic acid, 0.1M sodium acetate solution) for 30 minutes. Stain the cells.

After that, the plate was thoroughly washed with water, and after drying, 0.058-N
The dye is eluted with an aOH aqueous solution, the absorbance at 630 nm is measured, and the number of proliferating cells is determined from a quantitative curve showing the correlation between the absorbance measured in advance and the number of cells.

An example of the measurement results of the cell activation effect test of the aqueous extract of Ningyotake obtained by the method described above is as shown in FIG. FIG. 1 is a diagram illustrating the cell activating effect of each final concentration of the Ningyotake water extract, with the cell activating effect of the blank (no additives: control) taken as 100. This figure 1 suggests that any of the sections to which N. gyotake water extract is added has a cell activating effect, especially the 5% added portion (final concentration) and the 10% added portion of N. gyotake aqueous extract. (final concentration), it was observed that the cell activation effect was approximately 3.0 to 3.1 times as significant as that of the blank area (no addition: control).

① Example of a test for measuring the antioxidant effect (antioxidant potency) of an extract extracted from Ningyotake with water and/or an organic solvent.

(1) Preparation of Ningyotake extract: Crush Ningyotake fruiting body (dry product) Ig, add 100 ml of extraction solvent, and repeat the reflux heating extraction operation for about 1 hour on a hot water bath three times. After the extraction, the extraction solvent of each extract is distilled off under normal pressure or reduced pressure, or evaporated to dryness to obtain dried products of various extracts of N. chinensis. In this example, water, ethanol, and ethyl acetate were used as extraction solvents. When water, methanol, and ethyl acetate were used as the extraction solvents, the yield of each extract in this example was approximately 30% ~45%, methanol extract ca.
3-33%, ethyl acetate extract about 15-23%.

(2) Preparation of sample solution for measurement: All of the extracts of Ningyoutaga prepared in (1) above are highly soluble in water. Therefore, each of the extracts of N. chinensis prepared in (1) above was dissolved in distilled water to a final concentration of 0.2% to prepare respective solutions and used as sample solutions for measurement. In this example, regarding the sample solutions for each measurement of each extract of Ningyotake, a 0.2% aqueous solution of water extract is hereinafter referred to as "Ningyotake water extract", and a 0.2% aqueous solution of methanol extract is hereinafter referred to as "Ningyotake". A 0.2% aqueous solution of ethyl acetate extract is hereinafter referred to as ``Ningyotaga ethyl acetate extract''.

In addition, as a negative control, no additives (blank: distilled water) were used.
A 0.2% aqueous solution of scorbic acid is used as a positive control.

(3) Method for measuring antioxidant effect (antioxidant potency): The antioxidant effect (antioxidant potency) is measured based on the known rhodan iron property.

This example uses linoleic acid as the standard oxidized sample. Then, ammonium thiocyanate reagent, oxidized linoleic acid, and ferrous chloride reagent are reacted to produce red-colored rhodanic iron, and absorbance at 500 nm is measured to determine the amount of rhodanic iron produced (red pigment quantity). Therefore, the more linoleic acid is oxidized, the higher the absorbance is, and when the oxidation of the standard sample to be oxidized (linoleic acid) is suppressed based on the antioxidant effect of the various extracts of N. chinensis described in (2) above, the oxidation of the standard sample (linoleic acid) is suppressed. The absorbance due to iron will show a low value.

In this example, 50
Measure the absorbance at 0 nm. Thereafter, in order to measure the antioxidant effect (potency) of each sample solution over time, each measurement sample solution was kept at 38°C and heated at a wavelength of 500°C every 24 hours.
The absorbance at nm is measured over time. Further, as a negative control (blank: control), distilled water is used as a measurement sample, and the absorbance is similarly measured over time.

(4) Examples of measurement results of the antioxidant effects (antioxidant potency) of various extracts of N. chinensis based on rhodan iron properties.

FIG. 2 is a diagram showing the results of measuring the antioxidant effect (antioxidant potency) of various extracts of Ningyotake, which were measured based on the above-mentioned rhodan iron properties.

FIG. 2 suggests that all of the various extracts of Ningyotake have an antioxidant effect that is approximately equal to or greater than that of ascorbic acid, a known antioxidant. In particular, the results suggest that the antioxidative effect of the ethyl acetate extract of N. gyotake is outstanding.

In addition, extracts from Ningyotake using organic solvents are
It is easily soluble in water and is suitable for a wide range of applications as a compounding agent in cosmetics.

■] The side of cosmetics containing extracts of each seed entity.

(1) Cosmetic cream <composition> (wt%)・Extract of fruiting body −・ −・～・−−5.0・Beeswax・−・
...
−−−−・・・−−・・・ 8.0・Squalane・
・ -----・ ・・10.0・Self-emulsifying glyceryl monostearate ・°゛ ・ 3.0・Polyoxyethylene cetyl ether (20B, 0) −・ −・・−1,0・
Propylene glycol °−−5.0・Potassium hydroxide−・・−−10.3・Fragrance−・−・・−・
・・−・ Appropriate amount/preservative/antioxidant ・−・−・
・Appropriate amount・Purified water・・−−・・・−・・・
−・ Remainder (2) Emulsion composition> (% by weight)・Extract of fruiting body・・・・−・−・・−−5,0・Squalane・・・・ −・ −・−・・... 8.0
・Vaseline・・・・・・・・・・・・・・・−・・−
・・・−−−−−・ 2.0・Beeswax・−・・・−
・・−−−・−・−・・−−−0,5・Sorbitan sesquioleate °゛0.8・Polyoxyethylene oleyl ether (20B, 0) ・Pa・・“°1.2・
Carboxyvinyl polymer −0,2・propylene glycol − ・−・5,0・potassium hydroxide −
・・ −・・ −0,Toethanol・−−,,−,,
,,,,,-,-7,0・Fragrance-----・--・・-
−Appropriate amount・Preservative・Antioxidant・°・Par・″ −Appropriate amount・Purified water・・−・−−−・・・・−−−One remainder (3
) Lotion composition> - Extract of fruiting body - Glycerin (% by weight) - 5,0 5.0 - Polyoxyethylene sorbitan monolaurate (20B, 0) - 1.
5.Ethanol-・-・----・10,0・
Fragrance・−・−・・−・−−−−1 −・−−−・
Appropriate amount/Preservative/Antioxidant −・ Appropriate amount/Legal pigment −・ Appropriate amount/Purified water ・ ・−
−−・−・−Remaining part (4) Composition of pack agent> (weight %)・Extract of fruiting body −・−・・−・5.0‐Vinyl acetate resin emulsion −15,0・Holyhinyl alcohol −
~ 10.0・Olive oil ・−・−−−・−−
-3,0・Glycerin・・−・−・−5,
0.Titanium oxide-・・-・・・-・-8,0・Kaolin −・・−・−・−7.0・Ethanol・−・・−−−−1・・5.0・Fragrance−1・・・
・・ ・ ---Appropriate amount/preservative/antioxidant-
・・・・Appropriate amount・Remaining purified water〈Effect of the invention〉 (1) Water or /
Each extract extracted with an organic solvent contains a substance that is safe and exhibits an excellent tyrosinase activity inhibiting effect.

Furthermore, the extract extracted from each of the seed bodies (all 6 types) with water or a water/organic solvent mixture contains the substance that exhibits the above-mentioned tyrosinase activity inhibiting effect, as well as the skin moisturizing effect. A viscous substance with a wetting effect is also included.

Therefore, cosmetics containing one or more extracts selected from the various extract groups extracted from each of the seed bodies (all six types) with water and/or organic solvents are excellent ( The present invention has remarkable effects in achieving the objects of the invention, such as providing an extremely excellent cosmetic having a) skin whitening effect or (b) skin whitening effect and skin moisturizing effect.

(2) In addition to the tyrosinase activity-inhibiting effect and skin moisturizing effect described in (1), the extract extracted from Ningyotake with water and/or an organic solvent has an excellent antioxidant effect.

Therefore, among the various extracts extracted from N. chinensis using a single organic solvent or a mixture of multiple organic solvents, the organic solvent extracts of N. chinensis include the above (1).
One of the organic solvent extracts selected from this group of Ningyotake mushroom organic solvent extracts because it has an excellent tyrosinase activity inhibiting effect, has excellent antioxidant effects, and is easily soluble in water and has an extremely wide range of applications in cosmetics. Cosmetics containing the above extracts not only have a skin whitening effect, but also prevent oxidation and deterioration of cosmetics, thereby improving the stability and safety of cosmetics. The present invention has a remarkable effect of achieving the object of the invention, such as providing a cosmetic having such effects.

Furthermore, the various extracts extracted from N. chinensis using water/organic solvent mixture extracts (N. It has both effects,
Moreover, it has an excellent antioxidant effect, is easily soluble in water, and has an extremely wide range of applications in cosmetics. Therefore, one or more extracts selected from the group of extracts extracted from Ningyotake using a mixture of water and organic solvents. Cosmetics that have been blended with cosmetics have a skin whitening effect and a skin moisturizing effect that makes the skin feel smooth and moist. It has remarkable effects in achieving the purpose of the invention, such as providing cosmetics that have various effects, such as preventing oxidation and deterioration, and improving the stability and safety of cosmetics. .

(3) In addition to the above-mentioned effects (1) and (2), the water extract of Ningyotake has an excellent cell activating effect.

Therefore, the water extract of Ningyotake contains the above-mentioned (1).
It has the tyrosinase activity inhibiting effect and the skin moisturizing effect, as well as the antioxidant effect mentioned in (2) above, and also has an excellent cell activating effect. Promotes the turnover of melanin pigments etc. deposited on the skin, and
Coupled with the tyrosinase activity inhibiting effect of the viscous substance, it has a particularly outstanding skin whitening effect, and also has an excellent effect on preventing and removing wrinkles on the skin based on the cell activation effect. It has a skin moisturizing effect that gives the skin a smooth and moist feel based on the skin moisturizing effect of
In addition, we provide cosmetics that have a variety of excellent effects, such as an outstanding effect on improving the stability and safety of cosmetics, such as preventing oxidation and preventing deterioration, based on antioxidant effects.
It has a remarkable effect of achieving the purpose of the invention.

[Brief explanation of the drawing]

FIG. 1 is a graph showing an example of test results for the cell proliferation effect (cell activating effect) of the water extract of Ningyotake. FIG. 2 is a graph showing an example of the results of a test on the antioxidant effect (antioxidant potency) of various extracts of Ningyotake. Patent Applicant Naris Cosmetics Co., Ltd. Agent Patent Attorney Takashi Komatsu Figure 1: Cell proliferation effect of water extract of Ningyotake mushroom Namicho test result example C@1210 Coefficient of comparison with <control> as 100 - D prison style) □ Reaction time (unit: M

Claims (1)

[Claims] (1) Polyporus conf
Luens Fr. ], Shogenji [Rozites]
caperata (Pers. ex Fr.)K
arst. ], Honshimeji [Lyophyllum s
himeji (Kawam.) Hongo], Russula lilacea Qu
'el], Ramaria
fumigata K. Yokoyama] and maitake [Grifola frondosa (Dic
ks. ex Fr. )S. F. 1. A cosmetic containing one or more extracts selected from the group of extracts extracted from each fruiting body of A. Gray with water and/or an organic solvent.