JPH0255034B2 - - Google Patents

Info

Publication number: JPH0255034B2
Authority: JP; Japan
Prior art keywords: enzyme; activity; substrate; casein; optimal
Prior art date: 1985-12-20
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired

Application number

JP28694485A

Other languages

English (en)

Japanese (ja)

Other versions

JPS62146594A (ja

Inventor

Keiji Takeuchi

Takashi Nishino

Hisao Shimogaki

Tahee Negi

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Lion Corp

Original Assignee

Lion Corp

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1985-12-20

Filing date

1985-12-20

Publication date

1990-11-26

1985-12-20 Application filed by Lion Corp filed Critical Lion Corp

1985-12-20 Priority to JP28694485A priority Critical patent/JPS62146594A/ja

1986-06-03 Priority to US06/870,018 priority patent/US4797362A/en

1986-06-05 Priority to CA000510910A priority patent/CA1297821C/en

1986-06-06 Priority to EP86107708A priority patent/EP0204342B1/de

1986-06-06 Priority to DK268686A priority patent/DK268686A/da

1986-06-06 Priority to DE8686107708T priority patent/DE3683802D1/de

1987-06-30 Publication of JPS62146594A publication Critical patent/JPS62146594A/ja

1990-11-26 Publication of JPH0255034B2 publication Critical patent/JPH0255034B2/ja

Status Granted legal-status Critical Current

Links

230000000694 effects Effects 0.000 claims description 49
239000000872 buffer Substances 0.000 claims description 30
108091005658 Basic proteases Proteins 0.000 claims description 21
239000000758 substrate Substances 0.000 claims description 13
QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 claims description 12
238000000034 method Methods 0.000 claims description 12
229910001424 calcium ion Inorganic materials 0.000 claims description 11
235000018102 proteins Nutrition 0.000 claims description 11
102000004169 proteins and genes Human genes 0.000 claims description 11
108090000623 proteins and genes Proteins 0.000 claims description 11
239000005018 casein Substances 0.000 claims description 8
BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 claims description 8
235000021240 caseins Nutrition 0.000 claims description 8
YBYRMVIVWMBXKQ-UHFFFAOYSA-N phenylmethanesulfonyl fluoride Chemical compound FS(=O)(=O)CC1=CC=CC=C1 YBYRMVIVWMBXKQ-UHFFFAOYSA-N 0.000 claims description 6
239000004094 surface-active agent Substances 0.000 claims description 5
238000000862 absorption spectrum Methods 0.000 claims description 4
239000003112 inhibitor Substances 0.000 claims description 4
QCVGEOXPDFCNHA-UHFFFAOYSA-N 5,5-dimethyl-2,4-dioxo-1,3-oxazolidine-3-carboxamide Chemical compound CC1(C)OC(=O)N(C(N)=O)C1=O QCVGEOXPDFCNHA-UHFFFAOYSA-N 0.000 claims description 3
KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims description 3
108010000912 Egg Proteins Proteins 0.000 claims description 3
102000002322 Egg Proteins Human genes 0.000 claims description 3
235000014103 egg white Nutrition 0.000 claims description 3
210000000969 egg white Anatomy 0.000 claims description 3
150000002500 ions Chemical class 0.000 claims description 3
YFZOUMNUDGGHIW-UHFFFAOYSA-M p-chloromercuribenzoic acid Chemical compound OC(=O)C1=CC=C([Hg]Cl)C=C1 YFZOUMNUDGGHIW-UHFFFAOYSA-M 0.000 claims description 3
239000000126 substance Substances 0.000 claims description 3
238000010521 absorption reaction Methods 0.000 claims description 2
238000001155 isoelectric focusing Methods 0.000 claims description 2
229910021645 metal ion Inorganic materials 0.000 claims description 2
MUCZHBLJLSDCSD-UHFFFAOYSA-N diisopropyl fluorophosphate Chemical compound CC(C)OP(F)(=O)OC(C)C MUCZHBLJLSDCSD-UHFFFAOYSA-N 0.000 claims 2
229960005051 fluostigmine Drugs 0.000 claims 2
238000002523 gelfiltration Methods 0.000 claims 1
102000004190 Enzymes Human genes 0.000 description 102
108090000790 Enzymes Proteins 0.000 description 102
229940088598 enzyme Drugs 0.000 description 102
239000000243 solution Substances 0.000 description 19
HEMHJVSKTPXQMS-UHFFFAOYSA-M sodium hydroxide Inorganic materials [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 16
239000003599 detergent Substances 0.000 description 13
239000000203 mixture Substances 0.000 description 9
239000007788 liquid Substances 0.000 description 7
DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 6
BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical class N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 6
239000002244 precipitate Substances 0.000 description 6
241000193830 Bacillus <bacterium> Species 0.000 description 5
241000194110 Bacillus sp. (in: Bacteria) Species 0.000 description 5
229910052921 ammonium sulfate Inorganic materials 0.000 description 5
235000011130 ammonium sulphate Nutrition 0.000 description 5
238000004140 cleaning Methods 0.000 description 5
238000010828 elution Methods 0.000 description 5
238000001641 gel filtration chromatography Methods 0.000 description 5
238000000746 purification Methods 0.000 description 5
238000005185 salting out Methods 0.000 description 5
LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
108091005804 Peptidases Proteins 0.000 description 4
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
241000894006 Bacteria Species 0.000 description 3
239000004471 Glycine Substances 0.000 description 3
102000011782 Keratins Human genes 0.000 description 3
108010076876 Keratins Proteins 0.000 description 3
239000004365 Protease Substances 0.000 description 3
235000001014 amino acid Nutrition 0.000 description 3
150000001413 amino acids Chemical class 0.000 description 3
239000001913 cellulose Substances 0.000 description 3
229920002678 cellulose Polymers 0.000 description 3
238000005119 centrifugation Methods 0.000 description 3
KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
239000004744 fabric Substances 0.000 description 3
239000008363 phosphate buffer Substances 0.000 description 3
241000894007 species Species 0.000 description 3
239000006228 supernatant Substances 0.000 description 3
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
229920000936 Agarose Polymers 0.000 description 2
VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 2
CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
102000035195 Peptidases Human genes 0.000 description 2
NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 2
CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 2
108010056079 Subtilisins Proteins 0.000 description 2
102000005158 Subtilisins Human genes 0.000 description 2
QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 2
238000001042 affinity chromatography Methods 0.000 description 2
238000005571 anion exchange chromatography Methods 0.000 description 2
239000007853 buffer solution Substances 0.000 description 2
238000006243 chemical reaction Methods 0.000 description 2
239000012459 cleaning agent Substances 0.000 description 2
238000001816 cooling Methods 0.000 description 2
238000000354 decomposition reaction Methods 0.000 description 2
230000000593 degrading effect Effects 0.000 description 2
238000000502 dialysis Methods 0.000 description 2
239000002609 medium Substances 0.000 description 2
238000011218 seed culture Methods 0.000 description 2
SQGYOTSLMSWVJD-UHFFFAOYSA-N silver(1+) nitrate Chemical compound [Ag+].[O-]N(=O)=O SQGYOTSLMSWVJD-UHFFFAOYSA-N 0.000 description 2
239000011780 sodium chloride Substances 0.000 description 2
239000011550 stock solution Substances 0.000 description 2
MRXDGVXSWIXTQL-HYHFHBMOSA-N (2s)-2-[[(1s)-1-(2-amino-1,4,5,6-tetrahydropyrimidin-6-yl)-2-[[(2s)-4-methyl-1-oxo-1-[[(2s)-1-oxo-3-phenylpropan-2-yl]amino]pentan-2-yl]amino]-2-oxoethyl]carbamoylamino]-3-phenylpropanoic acid Chemical compound C([C@H](NC(=O)N[C@H](C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC=1C=CC=CC=1)C=O)C1NC(N)=NCC1)C(O)=O)C1=CC=CC=C1 MRXDGVXSWIXTQL-HYHFHBMOSA-N 0.000 description 1
LJGHYPLBDBRCRZ-UHFFFAOYSA-N 3-(3-aminophenyl)sulfonylaniline Chemical compound NC1=CC=CC(S(=O)(=O)C=2C=C(N)C=CC=2)=C1 LJGHYPLBDBRCRZ-UHFFFAOYSA-N 0.000 description 1
UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 description 1
239000004382 Amylase Substances 0.000 description 1
102000013142 Amylases Human genes 0.000 description 1
108010065511 Amylases Proteins 0.000 description 1
108010087765 Antipain Proteins 0.000 description 1
239000004475 Arginine Substances 0.000 description 1
241000228212 Aspergillus Species 0.000 description 1
241000193375 Bacillus alcalophilus Species 0.000 description 1
241000194108 Bacillus licheniformis Species 0.000 description 1
244000063299 Bacillus subtilis Species 0.000 description 1
235000014469 Bacillus subtilis Nutrition 0.000 description 1
BTBUEUYNUDRHOZ-UHFFFAOYSA-N Borate Chemical compound [O-]B([O-])[O-] BTBUEUYNUDRHOZ-UHFFFAOYSA-N 0.000 description 1
108010059892 Cellulase Proteins 0.000 description 1
OLVPQBGMUGIKIW-UHFFFAOYSA-N Chymostatin Natural products C=1C=CC=CC=1CC(C=O)NC(=O)C(C(C)CC)NC(=O)C(C1NC(N)=NCC1)NC(=O)NC(C(O)=O)CC1=CC=CC=C1 OLVPQBGMUGIKIW-UHFFFAOYSA-N 0.000 description 1
108010038061 Chymotrypsinogen Proteins 0.000 description 1
102000018832 Cytochromes Human genes 0.000 description 1
108010052832 Cytochromes Proteins 0.000 description 1
241000223218 Fusarium Species 0.000 description 1
102000001554 Hemoglobins Human genes 0.000 description 1
108010054147 Hemoglobins Proteins 0.000 description 1
QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 1
ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 description 1
CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 1
HNDVDQJCIGZPNO-YFKPBYRVSA-N L-histidine Chemical compound OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 description 1
OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 1
102000004882 Lipase Human genes 0.000 description 1
108090001060 Lipase Proteins 0.000 description 1
239000004367 Lipase Substances 0.000 description 1
102000036675 Myoglobin Human genes 0.000 description 1
108010062374 Myoglobin Proteins 0.000 description 1
108010058846 Ovalbumin Proteins 0.000 description 1
240000004808 Saccharomyces cerevisiae Species 0.000 description 1
MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 1
229920002472 Starch Polymers 0.000 description 1
241000187747 Streptomyces Species 0.000 description 1
108090000787 Subtilisin Proteins 0.000 description 1
QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
108090001109 Thermolysin Proteins 0.000 description 1
MZVQCMJNVPIDEA-UHFFFAOYSA-N [CH2]CN(CC)CC Chemical group [CH2]CN(CC)CC MZVQCMJNVPIDEA-UHFFFAOYSA-N 0.000 description 1
238000002835 absorbance Methods 0.000 description 1
238000005273 aeration Methods 0.000 description 1
235000004279 alanine Nutrition 0.000 description 1
239000003513 alkali Substances 0.000 description 1
229910000147 aluminium phosphate Inorganic materials 0.000 description 1
235000019418 amylase Nutrition 0.000 description 1
SDNYTAYICBFYFH-TUFLPTIASA-N antipain Chemical compound NC(N)=NCCC[C@@H](C=O)NC(=O)[C@H](C(C)C)NC(=O)[C@H](CCCN=C(N)N)NC(=O)N[C@H](C(O)=O)CC1=CC=CC=C1 SDNYTAYICBFYFH-TUFLPTIASA-N 0.000 description 1
ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 1
235000003704 aspartic acid Nutrition 0.000 description 1
230000001580 bacterial effect Effects 0.000 description 1
OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 description 1
230000008033 biological extinction Effects 0.000 description 1
KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 description 1
239000004327 boric acid Substances 0.000 description 1
HITBOAGYESUOFH-UHFFFAOYSA-N boric acid hydrochloride Chemical compound Cl.OB(O)O HITBOAGYESUOFH-UHFFFAOYSA-N 0.000 description 1
238000011088 calibration curve Methods 0.000 description 1
229940106157 cellulase Drugs 0.000 description 1
238000004587 chromatography analysis Methods 0.000 description 1
108010086192 chymostatin Proteins 0.000 description 1
229910000365 copper sulfate Inorganic materials 0.000 description 1
ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 description 1
239000012228 culture supernatant Substances 0.000 description 1
238000012258 culturing Methods 0.000 description 1
235000018417 cysteine Nutrition 0.000 description 1
XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 1
ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 1
238000000921 elemental analysis Methods 0.000 description 1
239000003480 eluent Substances 0.000 description 1
238000011156 evaluation Methods 0.000 description 1
238000000855 fermentation Methods 0.000 description 1
230000004151 fermentation Effects 0.000 description 1
239000012530 fluid Substances 0.000 description 1
238000009472 formulation Methods 0.000 description 1
239000001963 growth medium Substances 0.000 description 1
238000010438 heat treatment Methods 0.000 description 1
HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 1
230000036571 hydration Effects 0.000 description 1
238000006703 hydration reaction Methods 0.000 description 1
238000011534 incubation Methods 0.000 description 1
239000004615 ingredient Substances 0.000 description 1
235000019421 lipase Nutrition 0.000 description 1
229910052943 magnesium sulfate Inorganic materials 0.000 description 1
235000019341 magnesium sulphate Nutrition 0.000 description 1
239000000463 material Substances 0.000 description 1
238000005259 measurement Methods 0.000 description 1
239000012528 membrane Substances 0.000 description 1
229960002523 mercuric chloride Drugs 0.000 description 1
LWJROJCJINYWOX-UHFFFAOYSA-L mercury dichloride Chemical compound Cl[Hg]Cl LWJROJCJINYWOX-UHFFFAOYSA-L 0.000 description 1
229910052751 metal Inorganic materials 0.000 description 1
239000002184 metal Substances 0.000 description 1
238000009629 microbiological culture Methods 0.000 description 1
244000005700 microbiome Species 0.000 description 1
229940092253 ovalbumin Drugs 0.000 description 1
230000003647 oxidation Effects 0.000 description 1
238000007254 oxidation reaction Methods 0.000 description 1
238000012856 packing Methods 0.000 description 1
235000019419 proteases Nutrition 0.000 description 1
230000007065 protein hydrolysis Effects 0.000 description 1
230000002797 proteolythic effect Effects 0.000 description 1
229940024999 proteolytic enzymes for treatment of wounds and ulcers Drugs 0.000 description 1
230000035484 reaction time Effects 0.000 description 1
238000011084 recovery Methods 0.000 description 1
150000003839 salts Chemical class 0.000 description 1
210000002374 sebum Anatomy 0.000 description 1
229910001961 silver nitrate Inorganic materials 0.000 description 1
229910052708 sodium Inorganic materials 0.000 description 1
239000011734 sodium Substances 0.000 description 1
229910000029 sodium carbonate Inorganic materials 0.000 description 1
CIJQGPVMMRXSQW-UHFFFAOYSA-M sodium;2-aminoacetic acid;hydroxide Chemical compound O.[Na+].NCC([O-])=O CIJQGPVMMRXSQW-UHFFFAOYSA-M 0.000 description 1
KVCGISUBCHHTDD-UHFFFAOYSA-M sodium;4-methylbenzenesulfonate Chemical compound [Na+].CC1=CC=C(S([O-])(=O)=O)C=C1 KVCGISUBCHHTDD-UHFFFAOYSA-M 0.000 description 1
239000008107 starch Substances 0.000 description 1
235000019698 starch Nutrition 0.000 description 1
230000001954 sterilising effect Effects 0.000 description 1
238000003756 stirring Methods 0.000 description 1
238000012360 testing method Methods 0.000 description 1
OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
229910052727 yttrium Inorganic materials 0.000 description 1

Landscapes

Detergent Compositions (AREA)
Enzymes And Modification Thereof (AREA)

JP28694485A 1985-06-06 1985-12-20 アルカリプロテア−ゼ Granted JPS62146594A (ja)

Priority Applications (6)

Application Number	Priority Date	Filing Date	Title
JP28694485A JPS62146594A (ja)	1985-12-20	1985-12-20	アルカリプロテア−ゼ
US06/870,018 US4797362A (en)	1985-06-06	1986-06-03	Alkaline proteases and microorganisms producing same
CA000510910A CA1297821C (en)	1985-06-06	1986-06-05	Alkaline proteases, microorganisms producing same and detergents
EP86107708A EP0204342B1 (de)	1985-06-06	1986-06-06	Alkalische Proteasen, diese produzierende Mikroorganismen und Reinigungsmittel
DK268686A DK268686A (da)	1985-06-06	1986-06-06	Alkalisk protease, mikroorganisme og fremgangsmaade til fremstilling af samme, samt detergent indeholdende proteaser
DE8686107708T DE3683802D1 (de)	1985-06-06	1986-06-06	Alkalische proteasen, diese produzierende mikroorganismen und reinigungsmittel.

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
JP28694485A JPS62146594A (ja)	1985-12-20	1985-12-20	アルカリプロテア−ゼ

Publications (2)

Publication Number	Publication Date
JPS62146594A JPS62146594A (ja)	1987-06-30
JPH0255034B2 true JPH0255034B2 (de)	1990-11-26

Family

ID=17710974

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
JP28694485A Granted JPS62146594A (ja)	1985-06-06	1985-12-20	アルカリプロテア−ゼ

Country Status (1)

Country	Link
JP (1)	JPS62146594A (de)

1985
- 1985-12-20 JP JP28694485A patent/JPS62146594A/ja active Granted

Also Published As

Publication number	Publication date
JPS62146594A (ja)	1987-06-30

Publication	Publication Date	Title
US4797362A (en)	1989-01-10	Alkaline proteases and microorganisms producing same
US5275945A (en)	1994-01-04	Alkaline proteases stable in heavy-duty detergent liquids
EP1050579B1 (de)	2006-04-26	Reinigungsmittelzusammensetzung
Dudhagara et al.	2014	Isolation, characterization and investing the industrial applications of thermostable and solvent tolerant serine protease from hot spring isolated thermophililic Bacillus licheniformis U1
US5474700A (en)	1995-12-12	Industrial alkaline protease from shipworm bacterium
JP2750789B2 (ja)	1998-05-13	洗浄剤組成物
JPH0255034B2 (de)	1990-11-26
JPH03997B2 (de)	1991-01-09
JPH01101884A (ja)	1989-04-19	新規アルカリプロテアーゼｌおよびその製造法
US20060142171A1 (en)	2006-06-29	Novel alkaline protease
Koike et al.	1996	NADP-specific glutamate dehydrogenase from alkaliphilic Bacillus sp. KSM-635: purification and enzymatic properties
JPH09299084A (ja)	1997-11-25	低温アルカリプロテアーゼ、それを生産する微生物、その製造法、並びに当該酵素を含有する洗浄剤組成物及び食品加工用酵素製剤
JPH0763366B2 (ja)	1995-07-12	新規プロテアーゼ
JPH0630578B2 (ja)	1994-04-27	アルカリセルラ−ゼｋ
JP3026111B2 (ja)	2000-03-27	新規アルカリプロテアーゼ及びその製造方法
JPH09299082A (ja)	1997-11-25	低温アルカリプロテアーゼｘ、それを生産する微生物、その製造法、並びに当該酵素を含有する洗浄剤組成物及び食品加工用酵素製剤
JP2509540B2 (ja)	1996-06-19	新規プロテア―ゼを産生する新規微生物
JPS63101493A (ja)	1988-05-06	アルカリプロテア−ゼ含有洗浄剤組成物
JPS63101495A (ja)	1988-05-06	アルカリプロテアーゼ含有洗浄剤組成物
JPS63101491A (ja)	1988-05-06	アルカリプロテア−ゼ含有洗浄剤組成物
JPS63101494A (ja)	1988-05-06	酵素含有洗浄剤組成物
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