JPH0288069A - Antibacterial deodorant - Google Patents

Abstract

(57) [Abstract] This bulletin contains application data before electronic filing, so abstract data is not recorded.

Description

[Detailed description of the invention] (Industrial application field) The present invention relates to a deodorizing agent having an antibacterial effect.

(Prior art) Currently known deodorizing methods include using air fresheners, activated carbon, and
Masking with zeolite, cyclodextrin, etc.
There are physical deodorizing methods that involve adsorption and inclusion, and chemical deodorizing methods that involve reacting with potassium dichromate, potassium permanganate, sulfuric acid, chlorine dioxide, chlorophyll, metal porphyrin, metal porphyrazine, etc. Furthermore, when microorganisms are involved in the generation of bad odors, a mixture of a deodorizer and an antibacterial agent is often used.

In general, deodorizers using chemical deodorizing methods are considered to be superior to physical deodorizing methods in terms of deodorizing efficiency, deodorizing sustainability, etc.; Metal porphyrin and metal porphyrazine having redox ability (JP-A-53-16384, JP-A-53-9
4293. Japanese Patent Publication No. 53-133590. Japanese Patent Publication No. 56-6
3355° JP-A-62142565) and chlorophyll are considered to be excellent in practical terms.

It is said that chlorophyllin, which has a porphyrin skeleton, has a bacteriostatic effect, but its effect is extremely weak; for example, the minimum inhibitory concentration against Staphylococcus is 4000.
p! /1ttl (T', A, Nevina
nd B, G, Bibby, J, Dental
Research, 33.

571-579, 1654).

On the other hand, although hematin is known as a biological component, its application has not been studied much, and Lanokas et al. investigated the possibility of hematin copper complex as an enzyme for the model reaction of cytochrome C oxidase. (
E., Lukas et., J.

Chem, Soc, Dalton Trans,
, 1982, p. 1035).

(Problems to be Solved by the Invention) As mentioned above, even metal porphyrins, etc., which are conventionally thought to have excellent deodorizing effects, do not work against bad odors caused by microorganisms, and it is necessary to separately add an antibacterial agent. be.

For example, a deodorizer with the ability to decompose foot odor has been created by adding silver ions, which have a bactericidal effect, to an iron phthalocyanine derivative that has a deodorizing effect ("Decomposing foot odor with enzymes", Nikkan Kogyo Shimbun, 1988 Article dated July 2). In any case, with the recent changes in the environment, there is a strong demand for deodorizers that are effective against all kinds of bad odors, and for this purpose, there is a need for deodorizers that have antibacterial effects and are also effective against bad odors caused by microorganisms. It becomes. However, at present, no chemical deodorizer that is safe and long-lasting and has excellent antibacterial activity has yet been found.

(Means for Solving the Problems) In order to find a new antibacterial deodorizer that is safe and long-lasting, the present inventors first focused on biological components from the viewpoint of safety, and focused on hematin, whose deodorizing effect has not yet been investigated. As a result of conducting various studies on and its metal salts, it was surprisingly discovered that it has an excellent deodorizing effect as well as a strong antibacterial effect.

As shown in Table 1, hematin or its metal complex exhibited a strong deodorizing effect against typical malodorous molecules such as ammonia, trimethylamine, hydrogen sulfide, and methyl mercaptan. The deodorizing effect of hematin and hematin metal complexes is thought to be due to the fact that they act as strong oxidation catalysts and chemically oxidize and remove malodorous molecules.

Furthermore, the present inventors have confirmed for the first time that hematin or its metal complex has a strong antibacterial effect. That is, as shown in Table 2, they were found to inhibit the growth of Staphylococcus aureus and Bacillus subtilis at a low concentration of 12.5ti97ml.

The present invention is based on this new knowledge, and is an antibacterial deodorizing agent containing hematin or a metal complex thereof.

Hematin is found in large quantities in human feces, especially when meat is eaten or gastric bleeding occurs, and it normally exists naturally in the form of a complex with proteins. Normally, hematin can be obtained by dissolving hemin in a dilute alkali, acidifying it with acetic acid, etc., and recrystallizing the precipitated crude hematin from pyridine or the like. Alternatively, the metal complex can be obtained by adding a solution of a metal salt corresponding to a dilute alkali of hematin, SumH.

Commonly used metal salts include copper sulfate, zinc sulfate, aluminum sulfate, antimony sulfate, cobalt sulfate, tin sulfate, lead sulfate, platinum sulfate, nickel sulfate, bismuth sulfate,
Magnesium sulfate, manganese sulfate, copper nitrate, zinc nitrate,
Magnesium nitrate, ferric chloride, copper phosphate, zinc acetate, copper acetate, magnesium acetate, calcium lactate, nickel lactate, etc. can be mentioned, but copper, iron, manganese, Nickel, cobalt, magnesium, tin, aluminum, or metal hydroxides or metal halides thereof are preferably used from the viewpoint of economy, safety, and the like. However, without being limited to these metals, any metal salt that forms a complex with hematin may be used.

The antibacterial deodorizer of the present invention is not particularly limited in its form in use, and may be in any form as long as its effects are exhibited. For example, it may be used as is, but
Water, activated carbon, zeolite, starch, sawdust, cyclodextrin, styrofoam, foamed concrete, foamed resin, pumice, various fibers, rubber, activated alumina, silica gel, diatomaceous earth, kaolin, molecular sieve, bentonite, dioxide as required. Dissolved, adsorbed, attached, impregnated or chemically applied to titanium, magnesium carbonate, magnesium oxide, adhesives, cellulose, gelatin, casein, gums, films, pulp, paper, leather, cloth, wood, minerals, cellophane, ceramics, plastics, etc. Can be used in combination.

Antibacterial deodorizers that are adsorbed, attached, impregnated, or chemically bonded to materials such as carbon fibers are used in a wide variety of applications depending on the purpose. For example, clothing supplies, various filters, disposable diapers, masks, various bats, underwear, bedding, socks, packaging, air purifiers, interior materials, various plastic bags, toiletry supplies, oral supplies, pet supplies, refrigerator supplies, soap. , detergents, slippers, sanitary products, carpets, toiletries, military supplies, artificial flowers, tobacco pipes, toothpaste, cosmetics, patient sheets, cotton,
Used in ink, paint, various sheets, mats, tissues, wet handkerchiefs, freshness-preserving paper, curtains, bran, shoji screens, fragrances, etc.

The deodorizing agent of the present invention may contain, if necessary, auxiliary additive components such as fillers, binders, auxiliary agents, and coloring agents that are commonly used in deodorizing agents for dishes.

(Example) Next, in order to specifically explain the present invention, examples will be given to show the effects, but the present invention is not limited to these examples in any way.

Example 1 1 g of hematin was dissolved in 100 ytt of chloroform,
This was adsorbed at 1% on 10 g of paper (Toyo Roshi Arashi 2), and 1y was taken from the dried product and used as a deodorizing agent sample.

Example 2 After dissolving hematin in 0.5 yxl, add 100 tons of pure water to dilute the solution. Separately, add a solution of copper nitrate trihydrate 751F in 25 ml of water and mix thoroughly. Adjust the pH to 6. with nitric acid.
9-70, remove the precipitated hematin copper complex, disperse it in washing machine water, and apply it to 2g of nonwoven fabric at 1% after drying.
11 was taken from the dried product and used as a deodorizing agent sample.

Example 3 5 ml of 1N caustic soda aqueous solution of hematin 200~
To the diluted solution by adding 100 ml of pure water, add a solution of 85 #Ig of ferric chloride hexahydrate dissolved in 25 ml of water and mix well, then adjust the pH to 5 with dilute nitric acid. ．．
8-5. 9, the precipitated hematin iron complex was taken out, dispersed in washing machine water, adsorbed on cotton 2y to a concentration of 1% after drying, and 1g was taken from the dried material and used as a deodorizer sample. .

Example 4 After dissolving hematin 200 M9 in 5 ml of 1N caustic soda aqueous solution, 100 ml of pure water was added to dilute the solution, and 75 ml of cobalt chloride hexahydrate was separately added to 25 ml of water.
After adding the solution containing 100 mg of nitric acid and mixing thoroughly, p
i-I 5. 9-6. 0, the precipitated hematin cobalt complex was taken out, dispersed in washing machine water, adsorbed on activated carbon 2y to a concentration of 1% after drying, and 1 g was taken from the dried product and used as a deodorizer sample. .

Example 5 Hematin 200 ~ 1 N-caustic soda aqueous solution 5 m
1, add 100 rnl of pure water to dilute the solution, and separately add 79N of manganese nitrate tetrahydrate to 25ml of water.
After adding saliva and mixing thoroughly, adjust the pH with dilute nitric acid.
6. 9-7. 0, the hematin manganese complex precipitated was removed, dispersed in washing machine water, adsorbed on silica gel 2y to a concentration of 1% after drying, and 1f/ was taken from the dried product and used as a deodorizer sample. did.

Deodorization test 1 g of the deodorizer sample prepared in Examples 1 to 5 was placed in a 2e volume Tetra bag, and after sealing, malodorous gases (ammonia, trimethylamine, hydrogen sulfide, methyl mercaptan) adjusted to a specified severity were added. After passing through 1e, the concentration of malodorous gas was measured over time using a gas detection tube (manufactured by Gastic Co., Ltd.). The measurement results are summarized in Table 1.

As is clear from these results, it was confirmed that the deodorizing agent of the present invention has a strong deodorizing effect against any malodorous gas and in any dosage form.

The following margin anti-14 test (1) Minimum growth inhibition C interlayer (MIC) measurement test EflO
Omq is commonly understood as 10ml of dimethyl sulfoxide.
0.000ti! A sample stock solution of J/shoulder l was prepared. This stock solution was diluted with dimethyl sulfoxide and s, o o
o, +, 000.500.250.125.100.
A dilution series of 50.25.125.1 μl/ml was prepared and used in the test. Aseptically take 1 ml of each sample stock solution and dilution series into a Petri dish, and add 9 ml of sensitive agar medium.
A plate medium was prepared by mixing with

The bacterial solution for testing is MIC specified by the Japanese Society of Chemotherapy.
It was prepared based on the measurement method (1980). The bacterial solution was applied one platinum loop at a time to the above-mentioned plate medium and cultured at 37°C for 24 hours, and the lowest temperature of 0°C at which no bacterial growth was observed was defined as the MIC. The test results are summarized in Table 2.

(2) Measurement Examples 1 to 5 by shake flask method
After sterilizing the deodorant sample prepared in step 1, a suspension buffer of the test bacteria was added, and the number of viable bacteria was counted after shaking at 150 times/min for 1 hour in a closed container. The reduction rate for the number of bacteria was determined. The test results are summarized in Table 3.

From the above results, it was found that the deodorizing agent of the present invention has excellent antibacterial action, and it was also confirmed that it is effective against bad odors caused by microorganisms.

Margin below (Effects of the invention) According to the present invention, it has a strong antibacterial effect, so it is effective against bad odors caused by microorganisms, and has a safe and long-lasting chemical deodorizing effect, making it extremely practical. A highly antibacterial deodorizer is provided.

Procedural Amendment (Voluntary) 1999' et al. 7th

Claims (1)

[Claims] An antibacterial deodorizer containing hematin or its metal complex.