JPH0315692B2 - - Google Patents
Info
- Publication number
- JPH0315692B2 JPH0315692B2 JP57090669A JP9066982A JPH0315692B2 JP H0315692 B2 JPH0315692 B2 JP H0315692B2 JP 57090669 A JP57090669 A JP 57090669A JP 9066982 A JP9066982 A JP 9066982A JP H0315692 B2 JPH0315692 B2 JP H0315692B2
- Authority
- JP
- Japan
- Prior art keywords
- membrane
- enzyme
- substrate
- membrane substrate
- electrode
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 239000012528 membrane Substances 0.000 claims description 88
- 108090000790 Enzymes Proteins 0.000 claims description 72
- 102000004190 Enzymes Human genes 0.000 claims description 72
- 239000000758 substrate Substances 0.000 claims description 53
- 239000000126 substance Substances 0.000 claims description 28
- VVJKKWFAADXIJK-UHFFFAOYSA-N Allylamine Chemical compound NCC=C VVJKKWFAADXIJK-UHFFFAOYSA-N 0.000 claims description 20
- 238000004519 manufacturing process Methods 0.000 claims description 17
- 239000011148 porous material Substances 0.000 claims description 17
- 239000000178 monomer Substances 0.000 claims description 8
- 230000002452 interceptive effect Effects 0.000 claims description 6
- 239000003795 chemical substances by application Substances 0.000 claims description 5
- 125000003277 amino group Chemical group 0.000 claims description 4
- 239000003431 cross linking reagent Substances 0.000 claims description 4
- 230000005684 electric field Effects 0.000 claims description 3
- 230000015572 biosynthetic process Effects 0.000 claims 1
- 230000005494 condensation Effects 0.000 claims 1
- 238000009833 condensation Methods 0.000 claims 1
- 229940088598 enzyme Drugs 0.000 description 51
- 239000010408 film Substances 0.000 description 11
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 10
- 238000000034 method Methods 0.000 description 9
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 6
- 235000010323 ascorbic acid Nutrition 0.000 description 5
- 229960005070 ascorbic acid Drugs 0.000 description 5
- 239000011668 ascorbic acid Substances 0.000 description 5
- 238000006116 polymerization reaction Methods 0.000 description 5
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 4
- 108010015776 Glucose oxidase Proteins 0.000 description 4
- 239000004366 Glucose oxidase Substances 0.000 description 4
- 239000008280 blood Substances 0.000 description 4
- 210000004369 blood Anatomy 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- 239000008103 glucose Substances 0.000 description 4
- 229940116332 glucose oxidase Drugs 0.000 description 4
- 235000019420 glucose oxidase Nutrition 0.000 description 4
- 239000002904 solvent Substances 0.000 description 4
- QOSSAOTZNIDXMA-UHFFFAOYSA-N Dicylcohexylcarbodiimide Chemical compound C1CCCCC1N=C=NC1CCCCC1 QOSSAOTZNIDXMA-UHFFFAOYSA-N 0.000 description 3
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 239000010409 thin film Substances 0.000 description 3
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- WABPQHHGFIMREM-UHFFFAOYSA-N lead(0) Chemical compound [Pb] WABPQHHGFIMREM-UHFFFAOYSA-N 0.000 description 2
- BASFCYQUMIYNBI-UHFFFAOYSA-N platinum Chemical compound [Pt] BASFCYQUMIYNBI-UHFFFAOYSA-N 0.000 description 2
- 229920000515 polycarbonate Polymers 0.000 description 2
- 239000004417 polycarbonate Substances 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- PWGJDPKCLMLPJW-UHFFFAOYSA-N 1,8-diaminooctane Chemical compound NCCCCCCCCN PWGJDPKCLMLPJW-UHFFFAOYSA-N 0.000 description 1
- 229920002134 Carboxymethyl cellulose Polymers 0.000 description 1
- 239000004743 Polypropylene Substances 0.000 description 1
- 108010092464 Urate Oxidase Proteins 0.000 description 1
- VJHCJDRQFCCTHL-UHFFFAOYSA-N acetic acid 2,3,4,5,6-pentahydroxyhexanal Chemical compound CC(O)=O.OCC(O)C(O)C(O)C(O)C=O VJHCJDRQFCCTHL-UHFFFAOYSA-N 0.000 description 1
- 239000007767 bonding agent Substances 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 239000003792 electrolyte Substances 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- 230000003100 immobilizing effect Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 210000004492 nuclear pore Anatomy 0.000 description 1
- 229920000620 organic polymer Polymers 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 239000012466 permeate Substances 0.000 description 1
- 229910052697 platinum Inorganic materials 0.000 description 1
- 229920006254 polymer film Polymers 0.000 description 1
- 230000000379 polymerizing effect Effects 0.000 description 1
- -1 polypropylene Polymers 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 239000004065 semiconductor Substances 0.000 description 1
- 229910052709 silver Inorganic materials 0.000 description 1
- 239000004332 silver Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/001—Enzyme electrodes
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP57090669A JPS58206960A (ja) | 1982-05-28 | 1982-05-28 | 酵素電極用酵素膜およびその製造方法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP57090669A JPS58206960A (ja) | 1982-05-28 | 1982-05-28 | 酵素電極用酵素膜およびその製造方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS58206960A JPS58206960A (ja) | 1983-12-02 |
| JPH0315692B2 true JPH0315692B2 (fr) | 1991-03-01 |
Family
ID=14004938
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP57090669A Granted JPS58206960A (ja) | 1982-05-28 | 1982-05-28 | 酵素電極用酵素膜およびその製造方法 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPS58206960A (fr) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2590802B2 (ja) * | 1984-10-12 | 1997-03-12 | 松下電器産業株式会社 | バイオセンサ |
| JP2590803B2 (ja) * | 1984-10-12 | 1997-03-12 | 松下電器産業株式会社 | バイオセンサ |
-
1982
- 1982-05-28 JP JP57090669A patent/JPS58206960A/ja active Granted
Also Published As
| Publication number | Publication date |
|---|---|
| JPS58206960A (ja) | 1983-12-02 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US7455874B2 (en) | Method for the fabrication of a biosensor comprising an enzyme electrode arrangement | |
| Scouten et al. | Enzyme or protein immobilization techniques for applications in biosensor design | |
| Tamiya et al. | Micro glucose using electron mediators immobilized on a polypyrrole-modified electrode | |
| US5326449A (en) | Composite membrane | |
| US4431507A (en) | Enzyme electrode | |
| EP0561966B1 (fr) | Electrode pourvue d'un revetement polymere auquel est liee une enzyme redox | |
| JP4790612B2 (ja) | 留置型分析センサ | |
| JP4055912B2 (ja) | グルコースセンサー | |
| EP0363504A1 (fr) | Procédé pour réaliser une couche comprenant un hydrogel à base de polyvinyle et une substance à activité biochimique sur un substrat | |
| Shi et al. | Site-selective lateral multilayer assembly of bienzyme with polyelectrolyte on ITO electrode based on electric field-induced directly layer-by-layer deposition | |
| JPS6212847A (ja) | 酵素/電極式中センサー | |
| WO2000063685A1 (fr) | Biocapteur | |
| JPH0328752A (ja) | 酵素固定化電極およびその製造方法 | |
| JPH0210902B2 (fr) | ||
| WO2006132250A1 (fr) | Biocapteur et cellule à biocapteur | |
| Strike et al. | Electrochemical techniques for the modification of microelectrodes | |
| JPS63182559A (ja) | 酵素電極の製造方法 | |
| Treloar et al. | Engineering the right membranes for electrodes at the biological interface; solvent cast and electropolymerised | |
| JP3122459B2 (ja) | 有機巨大分子またはバイオポリマーを重合体質の膜に固定する方法 | |
| JPH0315692B2 (fr) | ||
| JP3178429B2 (ja) | 尿糖計用センサ | |
| JPS59164953A (ja) | 固定化酵素膜およびその製造方法 | |
| JPS61274253A (ja) | 酵素膜およびその製造方法 | |
| Bridge et al. | Polyacrylonitrile Thin‐Film Composite Membranes for the Optimization of a Whole Blood Glucose Sensor | |
| JPS58211646A (ja) | 膜状酵素電極の製造法 |