JPH03167132A - Composition for buccal cavity - Google Patents
Composition for buccal cavityInfo
- Publication number
- JPH03167132A JPH03167132A JP1306571A JP30657189A JPH03167132A JP H03167132 A JPH03167132 A JP H03167132A JP 1306571 A JP1306571 A JP 1306571A JP 30657189 A JP30657189 A JP 30657189A JP H03167132 A JPH03167132 A JP H03167132A
- Authority
- JP
- Japan
- Prior art keywords
- present
- composition
- extract
- bacteria
- cuckoo
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000000203 mixture Substances 0.000 title claims abstract description 22
- 208000028169 periodontal disease Diseases 0.000 claims abstract description 19
- 239000004480 active ingredient Substances 0.000 claims description 15
- 239000000126 substance Substances 0.000 claims description 8
- 238000001256 steam distillation Methods 0.000 claims description 7
- 206010065716 Pharyngeal inflammation Diseases 0.000 claims 1
- 241000894006 Bacteria Species 0.000 abstract description 12
- 201000007100 Pharyngitis Diseases 0.000 abstract description 12
- 230000000694 effects Effects 0.000 abstract description 11
- 239000000284 extract Substances 0.000 abstract description 11
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 abstract description 9
- 238000000034 method Methods 0.000 abstract description 7
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 abstract description 6
- 210000003800 pharynx Anatomy 0.000 abstract description 6
- 235000015218 chewing gum Nutrition 0.000 abstract description 3
- 229940112822 chewing gum Drugs 0.000 abstract description 3
- 235000009508 confectionery Nutrition 0.000 abstract description 3
- 229940034610 toothpaste Drugs 0.000 abstract description 3
- 239000000606 toothpaste Substances 0.000 abstract description 3
- 125000003118 aryl group Chemical group 0.000 abstract description 2
- 201000006549 dyspepsia Diseases 0.000 abstract description 2
- 239000003208 petroleum Substances 0.000 abstract description 2
- 206010019345 Heat stroke Diseases 0.000 abstract 1
- 208000007180 Sunstroke Diseases 0.000 abstract 1
- 230000002265 prevention Effects 0.000 abstract 1
- 241000544061 Cuculus canorus Species 0.000 description 17
- 230000003239 periodontal effect Effects 0.000 description 10
- 241000193996 Streptococcus pyogenes Species 0.000 description 9
- 210000000214 mouth Anatomy 0.000 description 9
- 244000052769 pathogen Species 0.000 description 9
- 230000000844 anti-bacterial effect Effects 0.000 description 8
- 238000012360 testing method Methods 0.000 description 8
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 6
- 239000008280 blood Substances 0.000 description 6
- 210000004369 blood Anatomy 0.000 description 6
- 239000002609 medium Substances 0.000 description 6
- 241000196324 Embryophyta Species 0.000 description 5
- 230000002401 inhibitory effect Effects 0.000 description 5
- 235000013305 food Nutrition 0.000 description 4
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 3
- 241000606125 Bacteroides Species 0.000 description 3
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 3
- 239000002253 acid Substances 0.000 description 3
- 239000013543 active substance Substances 0.000 description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- WJLVQTJZDCGNJN-UHFFFAOYSA-N Chlorhexidine hydrochloride Chemical compound Cl.Cl.C=1C=C(Cl)C=CC=1NC(N)=NC(N)=NCCCCCCN=C(N)N=C(N)NC1=CC=C(Cl)C=C1 WJLVQTJZDCGNJN-UHFFFAOYSA-N 0.000 description 2
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 2
- 241000588877 Eikenella Species 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 206010061218 Inflammation Diseases 0.000 description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 2
- 229920002472 Starch Polymers 0.000 description 2
- JVOGSHDZLOJKKR-MXFMKSRJSA-I [Na+].[Na+].[Na+].[Mg++].CCc1c(C)c2cc3[n-]c(c(C)c3C=C)c(C)c3nc(C[C@H]3CCC([O-])=O)c(CC([O-])=O)c3[n-]c(cc1n2)c(C)c3C([O-])=O Chemical compound [Na+].[Na+].[Na+].[Mg++].CCc1c(C)c2cc3[n-]c(c(C)c3C=C)c(C)c3nc(C[C@H]3CCC([O-])=O)c(CC([O-])=O)c3[n-]c(cc1n2)c(C)c3C([O-])=O JVOGSHDZLOJKKR-MXFMKSRJSA-I 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 229960004504 chlorhexidine hydrochloride Drugs 0.000 description 2
- 229910052802 copper Inorganic materials 0.000 description 2
- 239000010949 copper Substances 0.000 description 2
- 208000002925 dental caries Diseases 0.000 description 2
- 229940088598 enzyme Drugs 0.000 description 2
- 239000000835 fiber Substances 0.000 description 2
- 239000003205 fragrance Substances 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 230000004054 inflammatory process Effects 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 229930014626 natural product Natural products 0.000 description 2
- 229940081974 saccharin Drugs 0.000 description 2
- 235000019204 saccharin Nutrition 0.000 description 2
- 239000000901 saccharin and its Na,K and Ca salt Substances 0.000 description 2
- 239000002904 solvent Substances 0.000 description 2
- 238000000638 solvent extraction Methods 0.000 description 2
- 239000008107 starch Substances 0.000 description 2
- 235000019698 starch Nutrition 0.000 description 2
- 239000006188 syrup Substances 0.000 description 2
- 235000020357 syrup Nutrition 0.000 description 2
- 239000003053 toxin Substances 0.000 description 2
- 231100000765 toxin Toxicity 0.000 description 2
- 108700012359 toxins Proteins 0.000 description 2
- 241000304886 Bacilli Species 0.000 description 1
- 241000485660 Capnocytophaga gingivalis ATCC 33624 Species 0.000 description 1
- 229920002134 Carboxymethyl cellulose Polymers 0.000 description 1
- 208000035473 Communicable disease Diseases 0.000 description 1
- 241000272177 Cuculiformes Species 0.000 description 1
- 108020004414 DNA Proteins 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241000605909 Fusobacterium Species 0.000 description 1
- 101710179002 Hemolytic toxin Proteins 0.000 description 1
- 241000207923 Lamiaceae Species 0.000 description 1
- QZXSMBBFBXPQHI-UHFFFAOYSA-N N-(dodecanoyl)ethanolamine Chemical compound CCCCCCCCCCCC(=O)NCCO QZXSMBBFBXPQHI-UHFFFAOYSA-N 0.000 description 1
- 206010048685 Oral infection Diseases 0.000 description 1
- 241001135209 Prevotella denticola Species 0.000 description 1
- 241000269821 Scombridae Species 0.000 description 1
- 241000194017 Streptococcus Species 0.000 description 1
- 101000684258 Streptococcus pyogenes Arginine deiminase Proteins 0.000 description 1
- 108010023197 Streptokinase Proteins 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- KGUHOFWIXKIURA-VQXBOQCVSA-N [(2r,3s,4s,5r,6r)-6-[(2s,3s,4s,5r)-3,4-dihydroxy-2,5-bis(hydroxymethyl)oxolan-2-yl]oxy-3,4,5-trihydroxyoxan-2-yl]methyl dodecanoate Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](COC(=O)CCCCCCCCCCC)O[C@@H]1O[C@@]1(CO)[C@@H](O)[C@H](O)[C@@H](CO)O1 KGUHOFWIXKIURA-VQXBOQCVSA-N 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 230000004596 appetite loss Effects 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- UOAITRWPAYGGAM-UHFFFAOYSA-L calcium;propane-1,2,3-triol;carbonate Chemical compound [Ca+2].[O-]C([O-])=O.OCC(O)CO UOAITRWPAYGGAM-UHFFFAOYSA-L 0.000 description 1
- 239000001768 carboxy methyl cellulose Substances 0.000 description 1
- 235000010948 carboxy methyl cellulose Nutrition 0.000 description 1
- 239000008112 carboxymethyl-cellulose Substances 0.000 description 1
- 239000000679 carrageenan Substances 0.000 description 1
- 235000010418 carrageenan Nutrition 0.000 description 1
- 229920001525 carrageenan Polymers 0.000 description 1
- 229940113118 carrageenan Drugs 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000004821 distillation Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000005194 fractionation Methods 0.000 description 1
- -1 glucose Sucrose fatty acid ester Chemical class 0.000 description 1
- 241000411851 herbal medicine Species 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- 235000021266 loss of appetite Nutrition 0.000 description 1
- 208000019017 loss of appetite Diseases 0.000 description 1
- 235000020640 mackerel Nutrition 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 235000019462 natural additive Nutrition 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 239000000419 plant extract Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- CVHZOJJKTDOEJC-UHFFFAOYSA-N saccharin Chemical compound C1=CC=C2C(=O)NS(=O)(=O)C2=C1 CVHZOJJKTDOEJC-UHFFFAOYSA-N 0.000 description 1
- 238000007127 saponification reaction Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 229960005202 streptokinase Drugs 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 229940032085 sucrose monolaurate Drugs 0.000 description 1
- 229940124597 therapeutic agent Drugs 0.000 description 1
- 239000001974 tryptic soy broth Substances 0.000 description 1
- 239000006150 trypticase soy agar Substances 0.000 description 1
- 108010050327 trypticase-soy broth Proteins 0.000 description 1
- UHVMMEOXYDMDKI-JKYCWFKZSA-L zinc;1-(5-cyanopyridin-2-yl)-3-[(1s,2s)-2-(6-fluoro-2-hydroxy-3-propanoylphenyl)cyclopropyl]urea;diacetate Chemical compound [Zn+2].CC([O-])=O.CC([O-])=O.CCC(=O)C1=CC=C(F)C([C@H]2[C@H](C2)NC(=O)NC=2N=CC(=CC=2)C#N)=C1O UHVMMEOXYDMDKI-JKYCWFKZSA-L 0.000 description 1
Landscapes
- Confectionery (AREA)
- Cosmetics (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
Description
【発明の詳細な説明】
［産業上の利用分野］
本発明は、天然植物起源の有効成分を含有する口腔用組
成物に関し、更に詳しくは、口腔内に存在する歯周病原
菌や咽頭内に存在する化膿レンサ球菌に作用し、それら
の細菌の生育を抑制して歯周病および咽の炎症の予防を
図る口腔用組成物に関する。Detailed Description of the Invention [Industrial Application Field] The present invention relates to an oral composition containing an active ingredient derived from natural plants, and more specifically, to a composition for oral cavity containing active ingredients derived from natural plants, and more specifically to periodontal pathogens present in the oral cavity and oral cavity compositions. The present invention relates to an oral composition that acts on Streptococcus pyogenes and inhibits the growth of these bacteria, thereby preventing periodontal disease and throat inflammation.
［従来の技術］ 關蝕と歯周病は口腔内の２大感染症である。[Conventional technology] Dental caries and periodontal disease are two major oral infections.
鯖蝕発生においては、５ｔｒｅ ｔＯｃＯｃｃｕｓ ｎ
ｕｔａｎｓが重要な役割を果たしていることが知られて
いる。一方、歯周病は、歯周病原性細菌の増加、細菌の
組繊内侵入、それらの感染に対する宿主応答の低下等が
その要因となり得ると考えられているが、正確な病因は
完全には把握されていない、しかし、歯周病患者の病巣
ム陰性桿菌の増加が認められ、歯周病は、それらの感染
症であることが示されつつある。In the occurrence of mackerel caries, 5tre tOcOccus n
It is known that utans play an important role. On the other hand, the causes of periodontal disease are thought to be an increase in periodontal pathogenic bacteria, the invasion of bacteria into tissue fibers, and a decrease in the host response to these infections, but the exact etiology is not completely clear. However, an increase in the number of focal negative bacilli in patients with periodontal disease has been observed, and periodontal disease is being shown to be an infectious disease for these patients.
咽の炎症に関しては、各種のレンサ球菌が関与し、中で
も、５ｔｒｅ ｔＯｃＯｃｃｕｓ ＯｅｎｅＳは、溶
血毒、デイック毒素、ストレプトキナーゼ、Ｄ Ｎ Ａ
分解酵素等の毒素および酵素を産生することが知られて
おり、各種の組繊に化膿性炎症を引き起こす。Regarding inflammation of the throat, various streptococci are involved, and among them, 5tre tOcOccus OeneS causes hemolytic toxin, Dick toxin, streptokinase, DNA
It is known to produce toxins and enzymes such as degrading enzymes, causing purulent inflammation in various tissue fibers.
したがって、歯周病や咽の炎症を予防・改善するために
は、これらの原因菌の生育を抑えることが重要であり、
有効な抗菌活性物質の開発・応用が望まれる。また、そ
のような抗菌活性物質は口腔内で使用されることから、
比較的安全性が高いと考えられる天然物であることが望
ましく、しがも原料となる天然物の品質が一定であるこ
とが望ましい。Therefore, in order to prevent and improve periodontal disease and throat inflammation, it is important to suppress the growth of these causative bacteria.
The development and application of effective antibacterial active substances is desired. In addition, since such antibacterial active substances are used in the oral cavity,
It is desirable that the natural product is considered to be relatively safe, and it is desirable that the quality of the natural product used as a raw material is constant.
これらの条件を踏まえ、鋭意研究を行った結果、ある種
の植物抽出物が、歯周病原菌および咽頭内に存在する化
膿レンサ球閑に対してその生育を抑制する抗菌活性を示
し、歯周病に有効であることを突き止め、本発明を完成
するに至った。Based on these conditions, we conducted extensive research and found that certain plant extracts have shown antibacterial activity to suppress the growth of periodontal pathogens and Streptococcus pyogenes present in the pharynx. The present invention has been completed based on the discovery that the method is effective.
［発明が解決しようとする課題］
本発明は、天然植物起源の有効成分を含有する口腔用組
成物であって、口腔内に存在する歯周病原菌や咽頭内に
存在する化膿レンサ球菌に作用し、それらの細菌の生育
を抑制して歯周病および咽の炎症の予防を図る口腔用組
成物を提供することを目的とする。[Problems to be Solved by the Invention] The present invention is an oral composition containing active ingredients derived from natural plants, which acts on periodontal pathogens present in the oral cavity and Streptococcus pyogenes present in the pharynx. An object of the present invention is to provide an oral cavity composition that suppresses the growth of these bacteria and prevents periodontal disease and throat inflammation.
［課題を解決するための手段］
本発明によれば、歯周病および咽の炎症予防に有効な口
腔用組成物であって、カッコウ（Ｐｏ ｏｓｔｅｎ＋ｏ
ｎ ｃａｂｌｉｎ Ｂｃｎｔｈａｍの地上部）から水蒸
気蒸留により抽出した低極性物質を有効成分として含有
することを特徴とする口腔用組成物が提供される。[Means for Solving the Problems] According to the present invention, there is provided an oral cavity composition effective for preventing periodontal disease and throat inflammation.
Provided is an oral composition characterized in that it contains as an active ingredient a low polar substance extracted by steam distillation from the above-ground part of N. cablin Bcntham.
カッコウは、Ｐａ ｏｓｔｅｌｌｏｎ ｃａｂｌｉｎ
Ｂｅｎｔｈａｌｌの地上部であり、日本薬局方外生薬規
格集（厚生省薬務局審査課監修）にも掲載されている安
全性の高い生薬である。主な用途としては、芳香性健胃
薬として、食欲不振、消化不良、暑気あたり等に効果が
あるとされている。The cuckoo is Pa ostellon cablin
It is the above-ground part of Benthall, and is a highly safe herbal medicine listed in the Non-Japanese Pharmacopoeia Standards for Crude Drugs (supervised by the Pharmaceutical Affairs Bureau, Ministry of Health and Welfare). Its main use is as an aromatic stomachic medicine, which is said to be effective against loss of appetite, indigestion, and hot weather.
本発明による口腔用組成物は好適には水蒸気蒸留により
抽出するが、低極性物質を効率よく抽出する他の方法も
用いることができ、例えば溶剤抽出法等によることもで
きる。The oral composition according to the invention is preferably extracted by steam distillation, but other methods for efficiently extracting low polar substances can also be used, such as by solvent extraction.
水蒸気蒸留法においては、通常の蒸留装置を用いて水蒸
気蒸留を行い、蒸留液を、ヘキサン、石油エーテル等で
抽出することにより、本発明による有効成分を得ること
ができる。In the steam distillation method, the active ingredient according to the present invention can be obtained by performing steam distillation using a conventional distillation apparatus and extracting the distillate with hexane, petroleum ether, or the like.
溶剤抽出法における溶剤としては、ヘキサン、エーテル
、酢酸エチル、アセトン、エタノール等の低極性物質を
溶解可能な溶剤を使用することができる。その他の抽出
方法においては、通常用いられるいずれの方法を用いて
もよい。As a solvent in the solvent extraction method, a solvent capable of dissolving a low polar substance such as hexane, ether, ethyl acetate, acetone, and ethanol can be used. As for other extraction methods, any commonly used method may be used.
例えば、カッコウ２Ｋ（＋を用いて水蒸気蒸留を行うと
、本発明による有効成分が２３ｇ程度得られ、これは次
のような性状を有する。For example, when steam distillation is performed using Cuckoo 2K(+), about 23 g of the active ingredient according to the present invention is obtained, which has the following properties.
外１ｉｊｌ：保かに黄味を帯びた粘稠性の液体比ｕ ：
ｄ ：：＝０．９５２６
屈折率： ｎ ’？）” ＝ １．５０７２比旋光度［
αコ己’＝−６．６７
酸価： ２１．７６９
ケン化価： ５２．６２３
水分： ０．１６％
本発明による有効成分を、チューインガム、キャンデイ
−錠菓、練りハミガキ、含喉刑等に配合することにより
、歯周病および咽炎症予防効果を有する食品を提供する
ことができる。Outside 1ijl: Slightly yellowish viscous liquid Ratio:
d::=0.9526 Refractive index: n'? )” = 1.5072 specific optical rotation [
αCo' = -6.67 Acid value: 21.769 Saponification value: 52.623 Moisture content: 0.16% The active ingredient according to the present invention is used in chewing gum, candy, tablets, toothpaste, etc. By incorporating these ingredients, it is possible to provide a food product that has the effect of preventing periodontal disease and pharyngitis.
本発明による有効成分を食品に添加する場合、最終濃度
がｏ、 ｏｏｏｉ〜１．０重量％となるように添加すれ
ば好適である。この範囲より低い添加量で添加すると効
果が不十分であり、この範囲より高い添加量で添加する
と味覚上不適切となる。When the active ingredient according to the present invention is added to foods, it is preferable to add it to a final concentration of o,oooi to 1.0% by weight. If added in an amount lower than this range, the effect will be insufficient, and if added in an amount higher than this range, the taste will be inappropriate.
［作用］
歯周病や咽の炎症の予防に有効な物質は既に幾つか知ら
れているが、口腔用組成物としてこの種の物質を開発す
るに際しては、特に安全性の観点から厳格な制限を考慮
する必要がある０本発明の有効成分の起源を天然植物で
あるカッコウに求めたのはこの理由による。[Effect] Several substances are already known to be effective in preventing periodontal disease and throat inflammation, but there are strict restrictions when developing these types of substances as oral compositions, especially from a safety perspective. It is for this reason that we sought the origin of the active ingredient of the present invention from the natural plant cuckoo.
カッコウはシソ科の植物であり、着香の目的で食品に添
加する天然添加物として公的に使用が許可されている。Cuckoo is a member of the Lamiaceae family, and is officially allowed to be used as a natural additive in food for flavoring purposes.
本出願人は、既にカッコウから所定の方法により抽出し
た抽出物が、カビおよび酵母を含む微生物に対して抗菌
性を示すことを突き止め、抗菌性組成物としてこれに関
する特許出願を行っているが（特願昭６３−１１８１９
８号）、同様のカッコウ抽出物が、歯周病や咽の炎症の
予防に有効であることを突き止め、本発明を完成するに
至った。この効果は、本出願により始めて開示されたも
のである。The applicant has already discovered that an extract extracted from cuckoos using a prescribed method exhibits antibacterial properties against microorganisms including mold and yeast, and has filed a patent application regarding this as an antibacterial composition. Patent application 1986-11819
No. 8), it was discovered that a similar cuckoo extract is effective in preventing periodontal disease and throat inflammation, leading to the completion of the present invention. This effect is first disclosed by this application.
カッコウを水抽出しても所定の効果を与える有効成分は
抽出されず、また特に水蒸気蒸留物が強い効果を有する
ことから（水蒸気蒸留では、低分子、低極性の物質しか
抽出されない）、低極性物質中に活性物質が存在すると
考えられる。また、前記特願昭６３−１１８１９８号に
おいて開示したように、カッコウ抽出物においては、酸
、塩基による分画により真菌であるカビおよび酵母に対
する抗菌性は向上するが、本発明が意図する細菌に対し
てはそのような効果は認められないことから、口腔用組
成物としてのカッコウ抽出物については、酸・塩基によ
る区分を考慮する必要はなく、この点を参酌すると、カ
ッコウ抽出物を抗菌性組成物として用いる場合と口腔用
組成物として用いる場合とでは、その作用は異なると推
定される。Extracting cuckoo with water does not extract the active ingredients that give the desired effect, and steam distillates have particularly strong effects (steam distillation extracts only low-molecular, low-polar substances). It is believed that an active substance is present in the substance. Furthermore, as disclosed in the above-mentioned Japanese Patent Application No. 118198/1983, cuckoo extract has improved antibacterial properties against fungi such as mold and yeast by fractionation with acids and bases; Since no such effect has been observed for cuckoo extracts as oral compositions, there is no need to consider the acid/base classification of cuckoo extracts. It is presumed that the effect is different when used as a composition and when used as an oral composition.
［発明の効果］
本発明によれば、天然植物起源の有効成分を含有する口
腔用組成物であって、口腔内に存在する歯周病原菌や咽
頭内に存在する化膿レンサ球閑に作用し、それらの細菌
の生育を抑制して歯周病および咽の炎症の予防を図る口
腔用組成物が提供される。[Effects of the Invention] According to the present invention, an oral composition containing active ingredients derived from natural plants acts on periodontal pathogens present in the oral cavity and streptococcus pyogenes present in the pharynx, An oral cavity composition is provided that suppresses the growth of these bacteria and prevents periodontal disease and throat inflammation.
［実施例］
以下に実施例により本発明を更に詳細に説明するが、本
発明は以下の実施例にのみ限定されるものではない。[Examples] The present invention will be explained in more detail with reference to Examples below, but the present invention is not limited only to the following Examples.
まず、本発明による有効成分（カッコウ水蒸気蒸留物）
の歯周病原菌および化膿レンサ球菌に対する抗菌活性を
調べた。First, the active ingredient according to the present invention (cuckoo steam distillate)
We investigated its antibacterial activity against periodontal pathogens and Streptococcus pyogenes.
試験培地として、次の２つの培地を用いた。The following two media were used as test media.
１丘玉亘韮１
Ｔｒｙｐｔｉｃａｓｅ ｓｏｙ ａｇａｒ （ＢＢＬ）
４０ ｇＨｅｎｉｎ （Ｓｉｇｌａ）
５ ｍｇＨｅｎａｄｉｏｎｅ （和光紬薬
） ０．Ｓｉｇ馬脱Ａｌｌ！１血？７Ｎ（−７
−５；ン） １００ ｌｌｌ蒸水水
９００１
°ヂ烏 ゛ ｌ立工
Ｔｒｙｐｔｉｃａｓｅ ｓｏｙ ｂｒｏｔｈ （ＢＢＬ
） ３０ ｇｔｌｅｎ＋ｉｎ （Ｓｉｇｔａ）
５ ｎｇＨｅｎａｄｉｏｎｅ （和
光紬薬） ０．５１ｇ蒸溜水
１０００ ｎ１本発明による口腔用組成物の抗菌
性試験は次のようにして行った。1 Trypticase soy agar (BBL)
40 g Henin (Sigla)
5 mg Henadione (Wako Tsumugi Pharmaceutical) 0. Sig horse escape All! 1 blood? 7N(-7
-5;n) 100 lll steam water
9001 ° も ゛ ゛ ゛ Trypticase soy broth (BBL
) 30 gtlen+in (Sigta)
5 ngHenadione (Wako Tsumugi Pharmaceutical) 0.51g distilled water
1000 n1 The antibacterial property test of the oral composition according to the present invention was conducted as follows.
常法により抽出したカッコウ水蒸気蒸留抽出物のろ過滅
菌（ミリポアフィルタ、
旧ＬＬＩＰＯＲＥ、０．２２１１ｍ）を行った後、２倍
希釈系列で前記血液平板培地に添加し、試験用血液平板
培地を作成した。細菌の前培養として血液平板培地に供
試菌を接種し、３〜４日間嫌気培養した。生育した各々
の細菌を少量の菌体懸濁用液体培地に懸濁し、菌体接種
用マルチイノキュレータの小試験管に移した。マルチイ
ノキュレータにより試験用血液平板培地に菌体を接種し
、４日間嫌気培養した。各濃度の試験用血液平板培地に
おけるそれぞれの菌体の発育の有無を判定し、最少発育
阻止濃度（Ｍ Ｉ Ｃ）を求めた。嫌気培養には嫌気ボ
ックスを用い、混合ガス（ＣＯ２；Ｉｔ□：Ｎ２＝１：
１：８）を注入し、３７゛Ｃで培養した。試験結果を、
歯周病原菌および化膿レンサ球菌（咽炎症菌）に対する
最少発育阻止濃度（ＭＩＣ）として第１表に示す。After performing filtration sterilization (Millipore filter, former LLIPORE, 0.2211m) of the cuckoo steam distilled extract extracted by a conventional method, it was added to the blood plate medium in a 2-fold dilution series to create a test blood plate medium. . As a preculture of bacteria, test bacteria were inoculated into a blood plate medium and cultured anaerobically for 3 to 4 days. Each of the grown bacteria was suspended in a small amount of liquid medium for cell suspension and transferred to a small test tube of a multi-inoculator for cell inoculation. The bacterial cells were inoculated into a test blood plate medium using a multi-inoculator and cultured anaerobically for 4 days. The presence or absence of growth of each bacterial cell in the test blood plate medium at each concentration was determined, and the minimum inhibitory concentration (MIC) was determined. An anaerobic box is used for anaerobic culture, and a mixed gas (CO2; It□:N2=1:
1:8) and cultured at 37°C. The test results,
Table 1 shows the minimum inhibitory concentration (MIC) against periodontal pathogens and Streptococcus pyogenes (pharyngitis).
匪上茎
菌株 Ｍ Ｉ Ｃ（μｓ／ｌｆ）歯周病関
連細菌
ＡＣｔｉｎＯｌｙＣｅＳ ｖｉｓｃｏｓｕｓ
３１３ＡＴＣＣ１５９８７
八ｃｔｉｎｏｎ＋ｙｃｅｓ ｎａｅｓｌｕｎｄｉｉ
６２５ＡＴＣＣ１２１０４
＾ｃｔｉｎｏｉｙｃｅｓ １ｓｒａｅｌｉｉ
６２５ＡＴＣＣ１２１０２
Ｐｒｏｐｉｏｎｉｂａｃｔｅｒｉｕｌｌａｃｎｅｓ
３ｉ３ＡｒＣＣ１１８２７
ｃａｐｎｏｃｙｔｏｐｈａｇａ ｇｉｎｇｉｖａｌｉｓ
ＡＴＣＣ３３６２４
Ｆｕｓｏｂａｃｔｅｒｉｕｎ ｎｕｃｌｅａｔｕｎ＾
ＴＣＣ２５５８６
Ｅｉｋｅｎｅｌｌａ ｃｏｒｒｏｄｃｎｓＦＤＣ３７
５
Ｅｉｋｅｎｅｌｌａ ｃｏｒｒｏｄｅｎｓＦＤＣ１０
７３
ＢａＣ１ｅｒＯｉｄｅＳ Ｑｉｒ＋ｇｉＶａｌｉＳＦＤ
Ｃ３８１
Ｂａｃｔｅｒｏｉｄｅｓ １ｎｔｅｒｎ＋ｅｄｉｕｓ
＾ＴＣＣ２５６１１
Ｂａｃｔｅｒｏｉｄｅｓ １ｎｔｅｒＰｅｄｉｌＪｓ
ＡＴＣＣ３３５６３
Ｂａｃｔｅｒｏｉｄｅｓ ｌ１ｅｌａｎｉｎＱ（ｌｅ４
１ｉｃＵｓＡＴＣＣ２５８４５
Ｂａｃｔｅｒｏｉｄｅｓ ｄｅｎｔｉｃｏｌａＡＴＣＣ
３３１８５
化膿レンサ球菌（咽炎症菌）
ｓｔｒｅｐｔｏｃｏｃｃｕｓ ｐｙｏｇｅｎｅｓ２５
１３
８
２５
５６
５６
５６
８
５６
２５
ＡＴＣＣ１０３９８
ｓｔｒｅｐｔｏｃｏｃｃｕｓ ｐｙｏｇｅｎｅｓ ＡＤ
Ｐ ６２５第１表に示したように、本発明によ
る有効成分（カッコウ水蒸気蒸留物）は、歯周病原菌お
よび化膿レンサ球菌に対して抑制作用を有し、特に歯周
病原菌として最も有力視されているＢａｃｔｅｒｏｉｄ
ｅｓ菌群に対して強い抑制作用を有することから、歯周
病治療剤として有用であることが分る。Stigmoid strain M I C (μs/lf) Periodontal disease-related bacteria ACtinOlyCeS viscosus
313ATCC15987 octinon+yces naeslundii
625ATCC12104 ＾ctinoiyces 1sraelii
625ATCC12102 Propionibacteriullacnes
3i3ArCC11827 capnocytophaga gingivalis
ATCC33624 Fusobacterium nucleatun＾
TCC25586 Eikenella corrodcnsFDC37
5 Eikenella corrodensFDC10
73 BaC1erOideS Qir+giValiSFD
C381 Bacteroides 1ntern+edius
^TCC25611 Bacteroides 1nterPedilJs
ATCC33563 Bacteroides l1elaninQ (le4
1icUsATCC25845 Bacteroides denticola ATCC
33185 Streptococcus pyogenes (pharyngitis) streptococcus pyogenes25 13 8 25 56 56 56 8 56 25 ATCC10398 streptococcus pyogenes AD
As shown in Table 1 of P625, the active ingredient (Cuckoo steam distillate) according to the present invention has an inhibitory effect on periodontal pathogens and Streptococcus pyogenes, and in particular, it has an inhibitory effect on periodontal pathogens and Streptococcus pyogenes, which is considered to be the most likely periodontal pathogen. Bacteroids
Since it has a strong inhibitory effect on the es bacteria group, it is found to be useful as a therapeutic agent for periodontal disease.
本発明による有効成分を、以下の実施例１〜５に示すよ
うにチューインガム、キャンデイ−１含喉剤、練り歯磨
、並びに錠菓に配合することにより、歯周病および咽炎
症予防効果を有する食品を製造した。Foods that have the effect of preventing periodontal disease and pharyngitis by incorporating the active ingredient according to the present invention into chewing gum, Candy-1 throat preparation, toothpaste, and tablet confectionery as shown in Examples 1 to 5 below. was manufactured.
ガムベース 砂糖 グルコース 水飴 ２０．０％ ５５．０ １５．０ ９．３ 香料 カッコウ ｌタ ４≦ 砂糖 水飴 クエン酸 カッコウ水蒸気蒸留物 エタノール 香料 銅クロロフイリンナトリウム サッカリン 塩酸クロルヘキシジン カッコウ水蒸気蒸留物 ０．５ ０．２ １００．０％ ５０．０％ ３４．０ １．０ Ｏ１２ １４，８ １００，０％ ３０．０％ １゜０ ０．１ ０．０５ ０．０１ ０．２ ６８．６４ １００．０％ 炭酸カルシウム グリセリン カラゲーナン カルボキシメチルセルロース ラウリルエタノールアマイド ショ糖モノラウレート カッコウ水蒸気蒸留物 銅クロロフイリンナトリウム 塩酸クロルヘキシジン サッカリン ５０゜０％ ２０．０ ０．５ １．０ １．０ ２．０ ０．２ ０．１ ０．０１ ０．１ ２５．０９ １００．０％ 砂糖 グルコース ショ糖脂肪酸エステル ７６．４％ １９、Ｏ Ｏ２１５ カッコウ水蒸気蒸留物 ０゜１ ｉｏｏ、ｏ％gum base sugar glucose starch syrup 20.0% 55.0 15.0 9.3 fragrance cuckoo lta 4≦ sugar starch syrup citric acid cuckoo steam distillate ethanol fragrance copper chlorophyllin sodium saccharin Chlorhexidine hydrochloride cuckoo steam distillate 0.5 0.2 100.0% 50.0% 34.0 1.0 O12 14,8 100,0% 30.0% 1゜0 0.1 0.05 0.01 0.2 68.64 100.0% calcium carbonate glycerin carrageenan carboxymethylcellulose lauryl ethanolamide sucrose monolaurate cuckoo steam distillate copper chlorophyllin sodium Chlorhexidine hydrochloride saccharin 50゜0% 20.0 0.5 1.0 1.0 2.0 0.2 0.1 0.01 0.1 25.09 100.0% sugar glucose Sucrose fatty acid ester 76.4% 19, O O215 cuckoo steam distillate 0゜1 ioo, o%
Claims (1)
であつて、カッコウ（￥Ｐｏｇｏｓｔｅｍｏｎ￥￥ｃａ
ｂｌｉｎＢｅｎｔｈａｍ￥の地上部）から水蒸気蒸留に
より抽出した低極性物質を有効成分として含有すること
を特徴とする口腔用組成物。(1) An oral composition effective for preventing periodontal disease and pharyngeal inflammation.
An oral composition comprising, as an active ingredient, a low polar substance extracted by steam distillation from the aerial part of Blin Bentham.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1306571A JPH03167132A (en) | 1989-11-28 | 1989-11-28 | Composition for buccal cavity |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP1306571A JPH03167132A (en) | 1989-11-28 | 1989-11-28 | Composition for buccal cavity |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH03167132A true JPH03167132A (en) | 1991-07-19 |
Family
ID=17958661
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP1306571A Pending JPH03167132A (en) | 1989-11-28 | 1989-11-28 | Composition for buccal cavity |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH03167132A (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5776462A (en) * | 1996-12-10 | 1998-07-07 | Sage R&D, A Partnership | Pogostemon cablin extract for inhibiting H. influenzae adhesion and treating otitis media or sore throat |
| WO2004110153A1 (en) * | 2003-06-17 | 2004-12-23 | Henkel Kommanditgesellschaft Auf Aktien | Agents against microorganisms, containing patchouli oil, patchouli alcohol and/or the derivatives thereof |
| US7557145B2 (en) | 2003-06-17 | 2009-07-07 | Henkel Kommanditgesellschaft Auf Aktien (Henkel Kgaa) | Inhibition of the asexual reproduction of fungi by eugenol and/or derivatives thereof |
| CN104116946A (en) * | 2014-07-03 | 2014-10-29 | 陈光秋 | Collateral-dredging sinew-relaxing liquid for external use |
| CN107126504A (en) * | 2017-05-17 | 2017-09-05 | 上海海虹实业(集团)巢湖今辰药业有限公司 | A kind of drop pill for pharynx health and preparation method thereof |
| CN107184925A (en) * | 2017-05-08 | 2017-09-22 | 蒋文 | Treat the Chinese medicine composition of chronic pharyngitis |
| CN108186947A (en) * | 2018-03-07 | 2018-06-22 | 李世辉 | A kind of Chinese medicine composition and its preparation method and application |
| CN108815498A (en) * | 2018-09-14 | 2018-11-16 | 中商国能孵化器集团有限公司 | A kind of pharmaceutical composition and the preparation method and application thereof for treating pharyngitis |
-
1989
- 1989-11-28 JP JP1306571A patent/JPH03167132A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5776462A (en) * | 1996-12-10 | 1998-07-07 | Sage R&D, A Partnership | Pogostemon cablin extract for inhibiting H. influenzae adhesion and treating otitis media or sore throat |
| WO2004110153A1 (en) * | 2003-06-17 | 2004-12-23 | Henkel Kommanditgesellschaft Auf Aktien | Agents against microorganisms, containing patchouli oil, patchouli alcohol and/or the derivatives thereof |
| US7557145B2 (en) | 2003-06-17 | 2009-07-07 | Henkel Kommanditgesellschaft Auf Aktien (Henkel Kgaa) | Inhibition of the asexual reproduction of fungi by eugenol and/or derivatives thereof |
| CN104116946A (en) * | 2014-07-03 | 2014-10-29 | 陈光秋 | Collateral-dredging sinew-relaxing liquid for external use |
| CN107184925A (en) * | 2017-05-08 | 2017-09-22 | 蒋文 | Treat the Chinese medicine composition of chronic pharyngitis |
| CN107126504A (en) * | 2017-05-17 | 2017-09-05 | 上海海虹实业(集团)巢湖今辰药业有限公司 | A kind of drop pill for pharynx health and preparation method thereof |
| CN108186947A (en) * | 2018-03-07 | 2018-06-22 | 李世辉 | A kind of Chinese medicine composition and its preparation method and application |
| CN108815498A (en) * | 2018-09-14 | 2018-11-16 | 中商国能孵化器集团有限公司 | A kind of pharmaceutical composition and the preparation method and application thereof for treating pharyngitis |
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