JPH03227935A - Production of powdery extract of medical ginseng - Google Patents

Abstract

PURPOSE:To effectively obtain the subject high-purity powder having a low moisture absorption by extracting a medical ginseng or its tissue culture material using water and/or a water-soluble organic solvent, concentrating the resultant extract to a specified solid matter concentration, subsequently treating the concentrated extract with a membrane having a specified differential molecular weight and carrying out spray drying. CONSTITUTION:A medical ginseng or its tissue culture material is extracted using water and/or a water soluble organic solvent such as ethanol and the resultant extract is concentrated to 5-50wt.% solid matter concentration. The concentrated extract is then treated with a membrane such as a reverse osmosis membrane (RO membrane) or an ultrafiltration membrane (UF membrane) having 10-10000, preferably 10-1000 differential molecular weight in order to remove low-molecular components. The resultant treated solution is subsequently spray dried, thus economically obtaining the objective medical ginseng extract powder of high purity useful as a food additive or a raw material of health foods and medicines, having a very low moisture absorption and capable of blending in a desired ratio for each purpose.

Description

[Detailed description of the invention] (Industrial application field) The present invention relates to a method for producing medicinal ginseng extract powder.

BACKGROUND OF THE INVENTION Medicinal ginseng, especially Panax ginseng (Panaxj. C, A, Meyer), is widely used in Chinese medicine. In ancient times, it was known for its medicinal properties such as tonicity and longevity, but now it is used to treat gastrointestinal disorders and diabetes.

It is used for stress etc. Medicinal ginseng extracts have been known for obtaining various medicinal effects of medicinal ginseng. From the form of the extract, there are two types: liquid extract (50% ethanol solvent) and soft extract (solid content 60-80 wt/wt%).
It is broadly divided into. Furthermore, extract powder is prepared because it is easy to handle and drink. However, even if the solvent in the extract is removed and powdered, the components contained in it are highly hygroscopic, so it cannot be easily powdered.
The yield of the powdered extract is extremely poor and the cost is high. Furthermore, even if an extract powder is obtained at a high yield by, for example, freeze-drying, the obtained powder is highly hygroscopic and easily absorbs moisture in the air and becomes starch syrup-like. Various efforts have been made to solve these problems. For example, there is a method of adding lactose or the like to medicinal carrot extract and drying it to obtain extract powder. The hygroscopicity of the extract powder obtained by adding this lactose is relatively low, but in order to store the extract powder for a long period of time without absorbing moisture, the extract content in the extract powder must be about 10% by weight or less. be. Therefore, highly pure medicinal ginseng extract powder cannot be obtained.

JP-A No. 54-80463 discloses a medicinal carrot extract powder obtained by adding 0.5 to 15% by weight of cyclodextrin to medicated carrot extract and drying it by aeration or under reduced pressure. . Because it contains cyclodextrin, this extract powder masks the bitterness of medicinal ginseng, and at the same time, as with lactose, a slight improvement in hygroscopicity is observed. However, its hygroscopicity is still insufficient, and when exposed to the air, it absorbs moisture over time and changes into a starch syrup-like appearance. As described above, medicinal ginseng powder with high purity and no hygroscopicity has not been obtained.

(Problem to be solved by the invention) The present invention solves the above-mentioned conventional drawbacks.

The purpose is to provide a method for producing a medicinal carrot extract powder that has extremely low hygroscopicity, high purity, and can contain medicinal carrot extract in a desired proportion depending on the purpose. Another object of the present invention is to provide a method for producing medicinal ginseng extract powder having the above-mentioned properties at low cost.

(Failure to solve the problem) The method for producing medicinal ginseng extract powder of the present invention is as follows.

A step of extracting medicinal ginseng or a tissue culture of medicinal ginseng with water and/or a water-soluble organic solvent, a step of concentrating the obtained extract into an extract with a solid content concentration of 5 to 50% w/w; The extract has a molecular weight cutoff of 10 to 10,
000 membrane to remove low-molecular components, and spray-drying the resulting treatment solution, thereby achieving the above object.

Types of medicinal ginseng used in the present invention include Panax ginseng, Panax ginseng, American ginseng, Panax ginseng, and Siberian ginseng. In order to obtain extracts from these carrots, for example, the raw carrot roots or dried white or red ginseng products are extracted using an appropriate solvent as described below. Here, white ginseng refers to raw medicinal carrots that are peeled and dried under the sun or heat, and red ginseng is raw medicinal carrots that are peeled and dried in the sun or heat after being treated with boiling water or steam and then dried in the sun or heat. This is what I did.

Instead of the above-mentioned medicinal carrot roots, a cell mass (callus) obtained by tissue culturing them may be used. To perform tissue culture of medicinal carrots, first, the tissue of the medicinal carrots is cut out and a callus is generated using a normal plant tissue culture method. next.

The obtained callus is subjected to static culture or liquid culture and allowed to proliferate. Liquid culture is particularly suitable. This callus can be grown indefinitely in culture.

Culture conditions do not need to be particularly special. As a medium,
Murashigeskoog (Mu) commonly used for plant tissue culture
rashige-3koog) medium, white (Wh
ite) medium, O, L, Ganpog (0,L.

Amborg) B5 medium, Nitsch
), Healer's medium, Morel (M
orel) culture medium can be used. If necessary, casein degrading enzyme, soybean flour, cornstarch liquor, vitamins, etc. may be added to this.

Callus (cell group or cell mass) obtained by liquid culture is collected by means such as filtration. These cells exist from single cells as the smallest unit to cell clusters as the largest unit. The cell mass does not become extremely large due to shaking or stirring in liquid culture, and the maximum diameter is about 15 mm at most. Callus obtained by static culture or liquid culture is dried as necessary. Normally, dry callus (granules with a water content of 10% by weight or less) is obtained by gently drying raw callus in the sun or under warm air of 30°C or higher, preferably 50 to 80°C. .

The medicinal ginseng extract powder of the present invention is prepared using the medicinal ginseng or its tissue culture. First, extraction is performed by adding the following extraction solvent to the medicinal carrot root or callus (both fresh or dried).

In order to perform extraction efficiently, it is preferable to use the carrot root or callus after crushing it. As the extraction solvent, water and/or a water-soluble organic solvent is used. Examples of water-soluble organic solvents include ethanol, methanol, and acetone. The obtained medicinal ginseng extract powder is used as an extraction solvent because it is applied to the human body as a raw material for pharmaceuticals and cosmetics.

Particularly preferred is non-toxic ethanol. The concentration of the water-soluble organic solvent may be in the range of 0 to 100%. The amount of extraction solvent is not particularly limited, but medicinal carrot roots or callus used for extraction (both fresh or dried)
It is usually 3 to 200 parts by volume per part by weight. The extraction temperature is usually 5 to 70°C, preferably 30 to 50°C. Extraction is possible even at temperatures lower than 5°C, although the extraction time will be longer.

If the extraction temperature is extremely high, there is a risk that the contained ginsessides will decompose. It is well known that when the extraction temperature increases by 10°C, the extraction rate approximately doubles. In order to perform extraction, in addition to the above-described method of leaving the mixture still, a method of stirring or shaking the extraction solvent may be adopted as necessary.

The components of the extracted carrot extract vary depending on the type of extraction solvent used. For example, when the extraction solvent is a 0 to 50% ethanol aqueous solution, ginsside is extracted in a constant amount regardless of the ethanol concentration. When the ethanol concentration exceeds 50%, the amount of extracted ginsside decreases, and the amount of extracted steroid-like substance, which is thought to be sapogenin, increases. When water is used as an extraction solvent, steroid-like substances are not extracted, but B group vitamins are extracted. In this way, an extract containing medicinal ginseng extract is obtained.

The extract of medicinal ginseng thus obtained is concentrated if necessary, and preferably adjusted to have a solid content concentration of 5 to 50% by weight/weight (W/W%). For example, medicinal ginseng extract is concentrated at 60-70°C under reduced pressure. If the solid content concentration is less than 5 W/W%, a long time is required in the membrane treatment step and the spray drying step, which will be described later, resulting in poor two-way workability.

If it exceeds 50 W/W%, the viscosity will increase, resulting in poor workability in the above steps, or.

Membrane treatment and drying become impossible.

The extract obtained by adjusting the solid content concentration in this way has a molecular weight cut-off of 2 from 10 to 10,000. Preferably 10~
1,000 to remove low molecular weight components. As the membrane, a reverse osmosis membrane (ROM'J), an ultrafiltration membrane (IF membrane), etc. are used. Molecular weight cut off is 1
When it is less than 0, many low-molecular components remain in the extract powder, so the medicinal carrot extract obtained has high hygroscopicity. On the other hand, if it exceeds 1.0.000, even the active ingredients in the medicinal ginseng extract will be removed, making it impossible to obtain excellent medicinal efficacy. for example.

When the extract is treated with a membrane module having a membrane with a molecular weight cutoff of 500, low molecular weight components with a molecular weight of 500 or less are removed from the extract.

The treatment liquid obtained by membrane treatment is powdered by spray drying. The temperature during spray drying is preferably 150 to 200'C. The yield of the medicinal ginseng extract obtained as a powder by spray drying (recovery rate based on the solid content in the above-mentioned treatment liquid subjected to spray drying) is 80% or more. The obtained medicinal carrot extract powder has extremely low hygroscopicity because the low molecular weight components have been removed.

It can be stored for a long time.

As described above, according to the method of the present invention, medicinal ginseng extract powder of stable quality and extremely low hygroscopicity can be obtained in high yield with high efficiency in one operation. The obtained medicated ginseng extract powder does not contain additives such as lactose and cyclodextrin and is highly pure. Therefore, the medicinal effects of medicinal ginseng can be obtained with just one small amount. The unique flavor of medicinal carrots is also retained. This powder is mixed with other excipients depending on the purpose.
It can be prepared into a powder with the desired extract content.

The obtained medicinal carrot extract powder can be added to various cooked foods, powdered drinks, etc. as a food additive, or can be widely used as a raw material for health foods and medicines.

(Example) The invention is illustrated by the following examples.

Implementation ↓ Grind the ancestor of Korean carrots (dried product) and make this pulverized product 1
50% ethanol was added at a ratio of 20 parts by volume per part by weight, and the mixture was allowed to stand at 40°C for 24 hours for extraction. The obtained extract was concentrated using an evaporator at 60°C to obtain an extract having a solid content concentration of 20 W/W%. The obtained extract was filtered using a filter paper (manufactured by Toyo Atvanchik Co., Ltd., No. 2). Next.

The filtrate was passed through a reverse osmosis membrane (manufactured by Nitto Denko Corporation, NTR75911R).
, molecular weight cut off: 500) to remove low molecular components to obtain a treated solution. The solid content concentration of the obtained treatment liquid was 4
It was 0%. This treatment liquid was spray-dried at 180°C to obtain a medicinal carrot extract powder. The yield (%) of the obtained powder was determined using a quadratic equation. The results are shown in the table below.

From the root of Panax ginseng, Murashige Skoog (Mur)
Callus was induced and cultured using a solid medium of ashige-5koog under normal conditions. next.

Murashige-Skoog's liquid culture medium 100ml in a liquid culture tank (capacity 150f)! was prepared and sterilized under high pressure.

1. Place the above callus on the medium of 2. Okg was inoculated, and agitation culture was performed with aeration for 2 weeks. The obtained cell mass was rotated at 60°.
It was dried with hot air at C. Using this dried Panax ginseng, an extract was extracted in the same manner as in Example 1 to obtain an extract powder. The yield of extract powder of the obtained culture was determined in the same manner as in Example 1.

The results are shown in the table below.

The procedure was the same as in Example 1 except that the treatment step using the Hokkai flame reverse osmosis membrane was not performed. The yield of the obtained extract powder is shown in the table below.

Comparison (2) The same as Example 2 except that the treatment step using a reverse osmosis membrane was not performed. The yield of the obtained extract powder is shown in the table below.

(Hereinafter in the margins) (Effects of the Invention) According to the present invention, as described above, a medicinal ginseng extract powder with high purity and low hygroscopicity is effectively prepared. The medicated ginseng extract powder obtained by the method of the present invention can be added to various cooked foods, powdered drinks, etc. as a food additive, or can be widely used as a raw material for health foods and medicines.

that's all

Claims (1)

[Claims] 1. A step of extracting medicinal ginseng or a tissue culture of medicinal ginseng with water and/or a water-soluble organic solvent;
A medicinal carrot extract comprising: concentrating the extract into an extract; treating the extract with a membrane having a molecular weight cutoff of 10 to 10,000 to remove low-molecular components; and spray-drying the obtained treatment liquid. Method of manufacturing powder.