JPH03228621A - Culture of spawn piece and culture device therefor - Google Patents

Abstract

PURPOSE:To secure production of Lentinus edodes by molding and culturing a mixture of sawdust and spawn packed into hole parts of a molded plate made of foamed synthetic resin to give spawn pieces, taking out the bottom of the hole part as a cover, inoculating the spawn on log for Lentinus edodes and obtaining the spawn pieces in high yield. CONSTITUTION:A mixture of sawdust and spawn in a loosened state is packed into a great number of bottomed hole parts 1 bored through a molded plate 5 made of foamed synthetic resin, molded and culture to produce a spawn pieces. Then the spawn pieces are taken out in an integrated state with bottom 2 of the hole part, the bottom part is immediately released from the molded plate, the spawn pieces with the bottom part 2 as a cover are embedded in log for Lentinus edodes such as pieces of wood and the spawn pieces are cultured. The above-mentioned method has excellent workability because the spawn pieces can be simply molded and cultured and covering operation of spawn cover is not required. The molded plate 5 and the cover have excellent heat retaining properties because of use of foamed synthetic resin and excellent air permeability because the spawn pieces are not wrapped with a cover during culture. Divided channels 8 are shown in the figure.

Description

[Detailed Description of the Invention] [Industrial Application Field] The present invention relates to a method and a culturing device for cultivating seed fungi used for cultivating mushrooms, and in particular to so-called sawdust seed fungi in which seed fungi such as shiitake mushrooms are mixed with sawdust. A culture method in which sawdust seed fungi are cultured and molded into hook-shaped seed fungi pieces to effectively culture them in a state with high planting efficiency so that they can be easily inoculated into inoculation holes dug in hodaki etc. This invention relates to a culture device.

[Conventional technology]

Conventionally, in order to culture sawdust seed fungi used to produce shiitake mushrooms in the form of seed pieces, a plastic mold side frame is placed on a substrate, the sawdust seed fungus is pressed, the lid is placed on the mold, and after culturing, the mold is removed from the substrate into a mold with a lid. Inoculate the removed wood as it is, or culture and grow the sawdust starter by inserting and molding the sawdust starter into a form board with a tapered hole with a bottom, covering it with a lid, and fitting it into the hole. The pieces were pushed out from the bottom, taken out, and the seed pieces were planted on the tree with the lid attached. (Equity FIfAss
-41811) Here, the inoculum piece in the present invention is a state in which the inoculum prepared by mixing bacteria such as shiitake mushrooms with sawdust is loosened into -H pieces, then filled into a mold, press-molded, cultured, and formed into a hook shape. This refers to the sawdust seed fungus.

[Problem to be solved by the invention]

Among the conventional methods for producing shiitake mushrooms, a method in which a hole is directly drilled in logs such as bare wood, the hole is pre-impregnated with Shiitake fungus, and Kuki plugs (Mikoma fungus) are inoculated. Rounded edges, poor workability during inoculation, and if inoculated onto wood, etc., the growth of the fungus may be slow or the inoculation status between the inoculation holes may vary, resulting in poor yield. There were problems such as low rates. In addition, the special public corporation Showa 5
8-41811 has the advantage that when the cultured seed bacteria pieces are inoculated into tree trunks, etc., the inoculation work is easy and the bacteria grow faster than the conventional method. As with the conventional method, there are many work steps for molding the inoculation pieces, such as the need to fit the inoculation lid on, and the workability is poor, and it is difficult to release the mold from the form board or veneer, etc., making it difficult to mold. There were problems such as the molded seed mold pieces becoming stuck, and the lid that was placed on the molded seed mold pieces falling off from the seed mold after being released from the mold, which deteriorated the yield of the seed mold pieces and the workability of inoculation.

The present invention has been developed in view of the various problems with the conventional culture method for culturing seed pieces such as shiitake mushrooms, etc., and has excellent workability, such as easily forming and culturing seed pieces, and not requiring the work of fitting an inoculation lid. Moreover, since it uses a foamed synthetic resin molded body, it has good heat retention.
In addition, since it is not covered with a lid, breathability is also good.

Therefore, the seed bacteria pieces can be stably cultured with little variation in culture between seed pieces, and the so-called bacteria breeding period can be shortened. It is possible to add a means to easily release the mold from the molded body, and in addition, there is very little separation of the lid from the seed mold, unlike in the conventional method, and the yield of the seed mold is improved. It is an object of the present invention to provide a method and a culturing device for culturing seed mushrooms, which can ensure a higher production amount of shiitake mushrooms than ever before because the workability of inoculation is improved.

[Means to solve the problem]

In order to achieve the above object, the present invention disintegrates and fills the holes of a foamed synthetic resin plate-shaped molded article with a large number of bottomed holes with sawdust starter, which is a mixture of starter and sawdust. After molding and culturing to produce a seed mold piece, when releasing the seed piece from the mold, the seed piece is taken out with the bottom of the hole attached to the seed piece, and is directly placed on raw wood such as oak. This is a method of cultivating a seed seed piece that can be inoculated using the bottom as a lid.The culture device used in the method of culturing the seed seed piece described above is a culture device in which the bottom is inserted into a through-hole portion of a plate-shaped molded product made of foamed synthetic resin. A seed culture device with a bottomed hole.

(b) Fit into the through-holes of a foamed synthetic resin plate-shaped molded body that has a number of through-holes without bottoms, and place it on the foamed synthetic resin plate so that it becomes the bottom of the hole. After fitting the plurality of protrusions provided into the through holes of the plate-shaped molded body, the protrusions fitted into the holes of the plate-shaped molded body were fitted into the holes of the plate-shaped molded body. A seed culture device that cuts the plate while leaving it intact.

(c) A plate-shaped molded product made of foamed synthetic resin having a large number of bottomed holes, and a V-shaped groove deeper than the bottom surface of the holes is provided along the side wall of the holes. . A seed culture device in which the plate-shaped molded body is cut in a direction perpendicular to the thickness direction at a level close to the deepest part of the cut groove. (b) or (c) above
In this case, the cutting is performed by hot-melting cutting using a heated nichrome wire or the like, so that the bottomed hole wall of the foamed synthetic resin plate-shaped molding and the hole bottom are fused by the foamed synthetic resin molten film layer formed at the time of cutting. A culturing device for seed bacteria pieces. (E) While the molded body with multiple grooves provided along the side wall of the hole is in a temperature range where it can be deformed, the deepest part of the V-shaped groove is pressed against the bottom surface of the molded body. A seed culture piece culture device arranged near the bottom surface of a shaped molded body. (f) Cut grooves along the outer and/or inner surfaces of the bottoms of the plurality of bottomed holes provided in the plate-shaped molded product made of foamed synthetic resin along the side walls of the holes are cut into holes. @Sl
A seed culture device in which the wall and the bottom are connected by a remaining thin film. (g) In the holes of the foamed synthetic resin plate-shaped molded product, cut grooves in an almost vertical direction from above the holes to reach the bottom of the holes, so as to cross and/or extend vertically and/or diagonally across the plurality of holes. Seed culture device set up by cutting. (→It also relates to a seed culture device in which a protruding body protrudes from the bottom of a hole of a bottomed foamed synthetic l11 resin plate-shaped molded body or an uneven portion is provided on the bottom of the hole.

The base material of the foamed synthetic resin molded body and the bottom of the seed culture device of the present invention is optimally foamed polystyrene, but any foamed polyolefin such as foamed polyethylene or foamed polypropylene or foamed polyurethane may be used. There is no problem in using foamed synthetic resin.

The seed culture device of the present invention is provided with protrusions or irregularities protruding inward from the bottom of the hole of the bottomed foamed synthetic resin molded body as shown in Figure 6 and @. This is to increase the contact surface area between the seed seed piece and the hole, increase the fixing strength due to the anchor effect of the seed piece, and at the same time reduce the amount of sawdust seed used per hole.

〔Example〕

Example 1 For example, about 400 bottomless through holes with an outer diameter of 13.5 fl are bored in a plate-shaped molded product made of foamed polystyrene, and grooves reaching the bottom are provided in the through holes, and then foaming is performed. The convex part of a plate-shaped body provided with a convex part that fits into the through-hole part made of polystyrene is fitted into the hole part of the plate-shaped molded product made of expanded polystyrene to form the bottom of the through-hole part, After filling the holes with sawdust starter and culturing it for 10 to 20 days after molding, the plate-shaped molded body was broken or deformed using the splitting groove, and the seed starter piece was taken out with the bottom still attached. . This inoculum piece with a bottom part could be easily embedded in a hole drilled in a wood with the bottom part as a lid, and the inoculum piece could be inoculated without losing its shape.

After molding and culturing, the inoculum pieces are integrated with the bottom of the plate-shaped molded body made of foamed synthetic wood Ql, but the amount of sawdust bacteria that can be packed into the holes of the plate-shaped molded body is to prevent separation of the inoculum pieces and the bottom. In order to reduce this, a convex body or a concavo-convex structure (FIG. 6, [F]) may be provided on the bottom of the hole toward the inside of the hole.

Next, the present invention will be explained with reference to the drawings.

FIG. 1 is a perspective view showing an entire embodiment of the culture device of the present invention in which grooves are cut in the lateral direction.

FIG. 2 shows a partial cross-sectional view of an embodiment of a sawdust seed culture device in which a bottomed hole is formed by back-fitting a bottom portion (2) into a through-hole portion 11) of a plate-shaped molded body made of foamed synthetic resin. bottom(
The plate-shaped molded body portion around 2) is omitted from the drawing.

Fig. 2 shows the through hole portions o>Gc of a plate-shaped molded product made of foamed synthetic W, which has a large number of through hole portions +1) without a bottom.
A plurality of protrusions (
2) into the through-hole (l) of the plate-shaped molded body to form the bottom (2) of the hole, and fit into the through-hole with the protrusion (2) remaining from the plate (3). This is a partial cross-sectional view showing an intermediate stage of an embodiment of a seed culture device in which a bottomed hole is formed by cutting the boundary between the combined plate (3) and the protruding object. To cut the protrusion (2) from the body (3) leaving it as the bottom of the through hole (1); 2), cut with a cutting tool such as a saw blade or knife blade, or by heating a nichrome wire or the like. The cutting may be performed by thermal melting or other means. Figure 4 shows a foamed synthetic resin plate-shaped molded product (5) with a large number of bottomed holes, and a 7-shaped or U-shaped cut groove (hereinafter simply a V-shaped cut groove) deeper than the bottom of the bottomed holes. Groove (4
)] is provided along the side wall of the hole, and the plate-shaped molded body (
6) is shown in a partial cross-sectional view showing the state before cutting of an example of the seed culture device for culturing the seed pieces created by cutting in the direction perpendicular to the thickness direction.

In this case too, foam synthesis is used! Any cutting method may be used to cut so as to form the bottom near the deepest part of the V-shaped groove provided along the side wall of the hole of the bottomed hole part made of II. However, when heated and cut with a nichrome wire cutter, the foamed synthetic resin melts and adheres or stays on the side wall and bottom of the hole near the V-shaped cut groove, and this molten resin adheres or fuses the image area. However, at the final stage of culture, the bottom part remains attached to the sawdust starter until the starter is removed.

FIG. 5 shows a foamed synthetic resin molded body (6) K provided with a plurality of nine bottomed holes, each of which has grooves (6) along the outer or inner surface of the bottom (7) along the circumference of the hole. (6) and the bottom surface (7) are shown in a partial sectional view of an embodiment of the seed culture device in which the bottom surface (7) is connected by the remaining thin film +6Y.

The thickness of this thin film (6Y) is such that the bottom part does not separate during inserting, molding, and culturing of the sawdust starter, and that any remaining particles attached to the starter pieces can be easily separated when deformed during mold release or when cut with the split groove. Figure 5 shows the case where the cut groove (6) is provided on the outer surface of the bottom, but even if the cut groove (6) is provided on the inner surface of the bottom, the cut can be made from both the outer and inner surfaces. The same effect can be achieved even if the groove (6) is provided.

Figure 6 shows a part of an example of a seed culture device in which a vertical groove (8) reaching the bottom is provided in the hole (1) of a plate-shaped molded body made of foamed synthetic W resin. Showing the front view, the sixth
The drawing shows a cross-sectional view thereof.

The split grooves (8) may be provided by cutting across and/or longitudinally and/or obliquely through the plurality of holes in the plate-shaped molded product made of foamed synthetic resin, or may be provided irregularly.

9747 (2), @ is the bottom surface (2) of the hole (5) of the bottomed foamed synthetic resin molded body (2) the protruding body (9) protruding inward from the K hole [Figure 7 (2) ] or uneven part 001 [Fig. 7 ■]
It has been established.

〔Effect of the invention〕

The effects of the present invention can be summarized as follows.

When growing seedlings such as shiitake mushrooms, the culture device of the present invention is made entirely of foamed synthetic resin, including the bottom, has a round shape, has good heat retention, and allows for good growth of seedlings, making it possible to shorten the cultivation period. . After culturing the seed seed pieces, the hole and bottom of the culturing device can be used as a cover when inoculating Hoda trees, so when culturing,
Compared to the conventional method that requires a lid to be fitted, the work process is omitted and the workability is good.Furthermore, if the culture device of the present invention is provided with a slot (8), the inoculum after cultivation can be easily removed from the culture device. When taking it out with the bottom still attached, the splitting grooves can be used as appropriate to break or deform it, and the effect is great. In addition, the structure of the side and bottom surfaces of the hole for culturing the seed culture pieces can be changed in various ways by having a bottom so that the seed pieces can be easily taken out. The anchoring effect strengthens the adhesion between the seeding piece and the bottom surface, and it has many effects such as being able to reduce the amount of sawdust seeding used in one hole.

[Brief explanation of drawings]

Fig. 1 is a partial cross-sectional view of an embodiment of a sawdust seed culture device in which a bottomed hole is formed by a culture bag of the present invention having a horizontal slot and a fitting K, and Fig. 3 shows a bottomed hole. Another foamed synthetic m8 is fitted into the through-holes of the plate-shaped molded product made of foamed synthetic resin, which has a large number of through-holes, and forms the bottom of the through-holes.
A seed culture piece culture device that is formed by fitting a plurality of protrusions provided on a plate made of m into a through hole of a plate-shaped molded body to form the bottom of the hole, and then cutting the plate part. FIG. 4 is a partial sectional view before cutting of one example, in which a V-shaped cut groove deep from the bottom of the bottomed hole is formed in a plate-shaped molded product made of foamed synthetic resin in which a number of bottomed holes are bored. The plate body is cut in the thickness direction at a level close to the deepest part of the V-shaped groove provided along the side wall of the hole, and a hole side wall is formed on the outer or inner surface of the bottom of the hole provided in the body. Figure 6 shows a partial cross-section of an embodiment of a seed culture device in which a cut groove is provided in the hole and the bottom is connected by a remaining thin film. At the bottom of the hole of the inoculum theater-shaped hole, which is provided with a groove that reaches almost the bottom through the hole, there is a protrusion projecting inward from the hole [Figure 7 (
4)] or an uneven portion [FIG. 7]] is shown. A partial sectional view of an embodiment of the seed culture device of the present invention is shown. 1. A through-hole portion of a plate-shaped molded product made of foamed synthetic resin 2, a bottom portion 3, a plate made of foamed synthetic resin provided with protrusions 4, a V-shaped cut groove 5, and a foamed synthetic resin plate with a bottomed hole. Resin plate-shaped molded body 6
, cut groove 6', thin film 7, bottom 81-divided groove 9, protrusion to, uneven part

Claims (1)

[Scope of Claims] 1. Sawdust starter, which is a mixture of starter and sawdust, is broken up and filled into the holes of a foamed synthetic resin plate molded product having a large number of bottomed holes, and then molded and cultured. After producing a seed mold piece, when releasing the seed piece from the mold, take out the seed piece while keeping the bottom of the hole attached to the seed piece, and place the bottom part as a lid on logs such as hodaki. 1. A method for cultivating seed germs, which is characterized in that they can be inoculated. 2. An apparatus for cultivating seed bacteria pieces, which is used in the method for culturing seed bacteria pieces according to claim 1, wherein the bottom part is backfitted into the through-hole part of the plate-shaped molded product made of foamed synthetic resin to form a bottomed hole part. 3. On another foamed synthetic resin plate so as to fit into the through-holes of the foamed synthetic resin plate-shaped molded body having a large number of through holes without bottoms so as to form the bottoms of the through-holes. After fitting the plurality of protrusions provided in the through-holes into the through-holes of the foamed synthetic resin plate-shaped molded body, the protrusions fitted into the holes of the lid plate-shaped molded body are 2. An apparatus for cultivating seed bacteria pieces, characterized in that it is used in the method for cultivating seed bacteria pieces according to claim 1, wherein said plate is cut while leaving an object fitted as the bottom of a hole with a through hole. 4. A plate-shaped molded product made of foamed synthetic resin that has a number of bottomed holes, and a V-shaped groove deeper than the bottom of the hole is provided along the side wall of the hole. , the seed culture used in the method for culturing seed culture pieces according to claim 1, which is obtained by cutting the plate-shaped molded body in a direction substantially perpendicular to the thickness direction at a level adjacent to the deepest part of the cut groove. Piece culture device. 5. The seed mold piece according to claim 3 or 4, wherein the cutting is carried out by hot melt cutting using a heated nichrome wire or the like, and the hole wall and the bottom are fused together by a foamed synthetic resin melt film layer formed at the time of cutting. Culture device. 6. By making a plate-shaped molded product made of foamed synthetic resin with a large number of bottomed holes, and by providing a V-shaped cut groove deeper than the bottom surface of the holes along the side wall of the holes. By pressing the bottom surface while the molded foamed synthetic resin plate-like molded product is in a deformable temperature range, the deepest part of the V-shaped kerf becomes the shape of the foamed synthetic resin plate-shaped molded product. A seed culture piece culturing device, characterized in that it is used in the method for culturing seed culture pieces according to claim 1, wherein the seed culture piece is arranged near the bottom surface. 7. Cut grooves along the side walls of the holes from the outer and/or inner surfaces of the bottoms of the plurality of bottomed holes provided in the plate-shaped molded product made of foamed synthetic resin, so that the side walls of the holes and the bottoms are aligned. A seed culture piece culture device characterized in that it is used in the seed culture piece culture method according to claim 1, which is connected by a remaining thin film. 8. In the holes of the foamed synthetic resin plate-shaped molded product, cut grooves in a substantially vertical direction from above the holes to reach the bottom of the holes, crossing and/or longitudinally and/or diagonally across the plurality of holes. 8. The seed culture device according to claim 2, 3, 4, 5, 6, or 7. 9. Claim 2 or 3 or 4 or 5 or 6 or 7 or 8, wherein the bottomed foamed synthetic resin plate-shaped molded body is provided with a protrusion or an uneven part that projects inward from the bottom surface of the hole. Seed culture device.