JPH0353677Y2 - - Google Patents

Description

[Detailed description of the invention] [Industrial application field] The present invention is a method for controlling the temperature inside the culture tank to a predetermined temperature in a culture tank used for culturing animal and plant cells and other microorganisms. This invention relates to a temperature control device for a culture tank.

[Prior art and its problems] Surrounding the outer periphery of the culture tank body with a jacket,
A culture tank in which a heat medium supply port and a heat medium discharge port are formed in this jacket, and a supply pipe line is connected to these supply ports and a discharge pipe line is connected to the discharge port has been known for a long time. In conventional culture tanks, in order to control the temperature inside the culture tank, a constant temperature tank with a considerable capacity is provided separately from the culture tank, and the heat medium adjusted to a predetermined temperature in this constant temperature tank is passed through the supply pipe. The heat medium was supplied into the culture tank from the pipe, and the excess heat medium was discharged from the discharge pipe.

In such conventional culture tanks, the outer periphery of the culture tank body is surrounded by a jacket, so the heat exchange efficiency with the culture tank body is good, but it is difficult to control the temperature of the culture solution inside the culture tank body. , it is necessary to install an external thermostatic chamber, which is not only uneconomical as the amount of heat medium to be circulated is large, but also because the temperature control is indirect control via the thermostatic chamber, the temperature change of the thermal medium is slow. However, depending on the type of cells to be cultured, there were problems such as not being able to perform the desired culture.

[Purpose and outline of the invention] The present invention solves the problems with the conventional ones, and by directly controlling the temperature of the heat medium inside the jacket, it is possible to control the temperature according to the culture conditions of the type of cells, etc. The purpose of this is to speed up the process, and by making the temperature control device into a unit, it is possible to use a single unit regardless of the size of the culture tank or jacket, thereby providing versatility. , the outer surface of the culture tank body is surrounded by a jacket, and this jacket has
It is characterized in that a temperature control unit equipped with a heater for heating the heat medium in the jacket, a cooling pipe for cooling the heat medium, and an air supply pipe for stirring the heat medium is installed in a detachable manner.

[Example] Hereinafter, an example of the temperature control device for a culture tank according to the present invention will be described based on the drawings.

A glass culture tank main body 2 is attached to one side of the glass jacket 1 having a substantially elliptical shape in the major axis direction.
The outer surface thereof is integrally formed with the inner surface of the jacket 1 with an appropriate distance therebetween.

On the flange 2a of the culture tank body 2, a lid body 4 having a stirring blade 3 in the center for stirring the culture solution a in the culture tank body 2 and a temperature sensor 10 on the side is attached to a clip 5. It is attached by.

On the other side of the jacket 1 in the longitudinal direction, there are a heater 8 for heating the heat medium b such as water in the jacket 1, and a cooling pipe 7 for cooling the heat medium b.
and a temperature control unit c to which air supply pipes 9 for stirring and temperature-uniforming the heat medium b are attached are removably installed.

In this example, the configuration described above is shown, but the number and capacity of the heaters 8, the size of the cooling pipe 7, etc. may be changed depending on the purpose of culturing or the type of cells, etc. It is obvious that it can also be changed.

[Function] Next, the function of the temperature control device of the above-described embodiment will be explained.

In order to raise the temperature of the culture solution a in the culture tank body 2, the heater 8 is operated.

Then, the heat medium b in the jacket 1 is heated by the heater 8, and the heat medium b in the jacket 1 and the culture solution a in the culture tank body 2 are heated through the large area of the outer peripheral surface of the culture tank body 2. Heat exchange is performed between them, and the temperature of the culture solution a is raised.

At this time, since the heater 8 directly heats only the heat medium b in the jacket 1, not only no extra heat capacity is required, but also the temperature of the culture solution a in the culture tank body 2 is rapidly raised.

During this time, by operating the air supply pipe 9, the heat medium b within the jacket 1 is maintained at a uniform temperature.

Further, when the culture solution a is heated to a predetermined temperature or higher, the temperature sensor 10 senses this, and an automatic control device (not shown) stops the operation of the heater 8, and cooling water is continuously supplied to the cooling pipe 7. As a result, the heat medium b in the jacket 1 is cooled, and the temperature of the culture solution a is rapidly lowered through the culture tank body 2.

When the temperature of the culture solution a is lowered to a predetermined temperature and the temperature sensor 10 senses this, the supply of cooling water to the cooling pipe 7 is stopped by the automatic control device.

Hereinafter, depending on the temperature change of the culture solution a, the heater 8
The operation and stopping of the cooling pipe 7 are repeated, and the culture solution a is maintained within a predetermined temperature range.

[Effects of the invention] As described above, the temperature control device for a culture tank according to the invention includes a heater that heats the heat medium in the jacket, a cooling pipe that cools the heat medium, and an air supply pipe that stirs the heat medium. Since it is configured as one temperature control unit and is detachably installed in the jacket, the heating medium inside the jacket is directly and uniformly heated and cooled, allowing for culturing without wasting heat. Not only can the temperature be controlled quickly according to the culture conditions of each cell, etc., but the equipment can be made smaller compared to those that require a constant temperature bath, and it can be used regardless of the size of the culture tank or jacket. First, it is possible to control the temperature of the culture solution by simply installing the temperature control unit in the jacket of the desired culture tank, making it convenient to handle and highly versatile. have

[Brief explanation of the drawing]

The drawings show an embodiment of the temperature control device for a culture tank according to the present invention, and FIG. 1 is an overall sectional view, and FIG. 2 is a perspective view of the temperature control unit. 1... Jacket, 2... Culture tank body, 7...
Cooling pipe, 8... Heater, 9... Air supply pipe, a
...Culture solution, b...heat medium, c...temperature control unit.

Claims (1)

[Scope of utility model registration request] The outer surface of the culture tank body is surrounded by a jacket, and the jacket includes a heater that heats the heat medium in the jacket, a cooling pipe that cools the heat medium,
1. A temperature control device for a culture tank, characterized in that a temperature control unit equipped with an air supply pipe for stirring a heat medium is detachably installed.