JPH0363292A - 電荷粒子の分離方法 - Google Patents

電荷粒子の分離方法

Info

Publication number
JPH0363292A
JPH0363292A JP1280033A JP28003389A JPH0363292A JP H0363292 A JPH0363292 A JP H0363292A JP 1280033 A JP1280033 A JP 1280033A JP 28003389 A JP28003389 A JP 28003389A JP H0363292 A JPH0363292 A JP H0363292A
Authority
JP
Japan
Prior art keywords
molecules
ion exchange
exchange material
column
separated
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP1280033A
Other languages
English (en)
Japanese (ja)
Inventor
Anthony R Torres
アンソニイ　アール　トレス
Elbert A Peterson
エルバート　エイ　ピーターソン
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of JPH0363292A publication Critical patent/JPH0363292A/ja
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J39/00—Cation exchange; Use of material as cation exchangers; Treatment of material for improving the cation exchange properties
    • B01J39/26—Cation exchangers for chromatographic processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Peptides Or Proteins (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP1280033A 1988-10-28 1989-10-30 電荷粒子の分離方法 Pending JPH0363292A (ja)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US263737 1988-10-28
US07/263,737 US5028696A (en) 1988-10-28 1988-10-28 Ion exchange and separation method

Publications (1)

Publication Number Publication Date
JPH0363292A true JPH0363292A (ja) 1991-03-19

Family

ID=23003035

Family Applications (1)

Application Number Title Priority Date Filing Date
JP1280033A Pending JPH0363292A (ja) 1988-10-28 1989-10-30 電荷粒子の分離方法

Country Status (5)

Country Link
US (1) US5028696A (de)
EP (1) EP0368092B1 (de)
JP (1) JPH0363292A (de)
AT (1) ATE85242T1 (de)
DE (1) DE68904732T2 (de)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5130027A (en) * 1989-09-19 1992-07-14 The University Of Colorado Foundation, Inc. Stationary magnetically stabilized fluidized bed for protein separation and purification
US5253981A (en) * 1992-03-05 1993-10-19 Frank Ji-Ann Fu Yang Multichannel pump apparatus with microflow rate capability
JP2669255B2 (ja) * 1992-04-01 1997-10-27 株式会社日立製作所 ヘモグロビン類の分析方法，分析装置およびそれに用いるカラム劣化抑制液
US6274348B1 (en) 1992-05-19 2001-08-14 Xoma Corporation Methods for the preparation of positively charged proteins
JP2773569B2 (ja) * 1992-09-17 1998-07-09 株式会社日立製作所 分離カラム、及び分離カラムの劣化防止方法，運搬方法，取扱方法そして分離カラム用封入液
US5545328A (en) * 1993-09-21 1996-08-13 Hemosol Inc. Purification of hemoglobin by displacement chromatography
CA2106612C (en) * 1993-09-21 2001-02-06 Diana Pliura Displacement chromatography process
US5478924A (en) * 1994-02-16 1995-12-26 Cramer; Steven M. Displacement chromatography of proteins using low molecular weight displacers
US5427686A (en) * 1994-04-05 1995-06-27 Sri International Process for separating material from mixture using displacement chromatography
GB9425138D0 (en) 1994-12-12 1995-02-08 Dynal As Isolation of nucleic acid
EP1147226B1 (de) * 1999-01-27 2013-01-23 Folim G. Halaka Materialien und verfahren zur reinigung von polyelektrolyten
CN1617938A (zh) 2002-01-16 2005-05-18 戴诺生物技术有限公司 从单个样品中分离核酸和蛋白质的方法
HUE033623T2 (en) 2002-09-11 2017-12-28 Genentech Inc protein purification
WO2004072647A1 (en) * 2003-02-07 2004-08-26 Beyond Genomics Removal of proteins from a sample
GB2407783A (en) * 2003-11-07 2005-05-11 Novexin Ltd Chromatographic separation
WO2006127973A2 (en) * 2005-05-24 2006-11-30 Waters Inverstments Limited Methods for separating and analyzing anionic compounds
KR101408026B1 (ko) * 2005-12-02 2014-06-20 사켐,인코포레이티드 음이온-교환 치환 크로마토그래피 공정 및 음이온-교환치환 크로마토그래피 공정에서 디스플레이서 화합물들로서사용하기 위한 음이온성 유기 화합물들
US8304248B2 (en) * 2009-11-16 2012-11-06 Torres Anthony R Protein separation via ion-exchange chromatography and associated methods, systems, and devices

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2694681A (en) * 1952-03-07 1954-11-16 Univ Idaho Res Foundation Inc Recycling the complexing agent in the separation of ions by ion exchange
GB862688A (en) * 1957-09-02 1961-03-15 Pawel Krumholz A process for separating ions by ion-exchange
GB1173996A (en) * 1967-05-10 1969-12-10 Hitachi Ltd Automatic Liquid Chromatography and Chromatograph
US4493907A (en) * 1981-06-22 1985-01-15 American Petro Mart, Inc. Method of using higher concentration sulfuric acid for stripping and precipitation of adsorbed magnesium
US5179199A (en) * 1986-10-20 1993-01-12 Genzyme Corporation Protein purification
US5118796A (en) * 1987-12-09 1992-06-02 Centocor, Incorporated Efficient large-scale purification of immunoglobulins and derivatives

Also Published As

Publication number Publication date
DE68904732T2 (de) 1993-08-12
US5028696A (en) 1991-07-02
ATE85242T1 (de) 1993-02-15
EP0368092B1 (de) 1993-02-03
EP0368092A1 (de) 1990-05-16
DE68904732D1 (de) 1993-03-18

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