JPH03981Y2 - - Google Patents

Description

[Detailed Description of the Invention] [Industrial Application Field] The present invention relates to a plate for cleaning microscopic bodies, and more particularly to a plate for cleaning microscopic bodies such as animal eggs.

[Conventional technology]

Generally, in experiments and research involving mammalian eggs, or in livestock fertilized egg transplantation projects, washing the eggs is essential.

Conventionally, such washing uses a plate in which a plurality of wells with a flat bottom are formed, each well is filled with a culture solution in advance, eggs are placed in the first well, and then eggs are placed in the first well. The eggs were sucked up with a Pasteur pipette and transferred to a second well, and the transfer operation was repeated successively to other wells to dilute impurities.

[Problem that the invention attempts to solve]

However, since the recesses of conventional plates had a flat bottom structure as described above, the eggs were scattered on the bottom surface, and when the Pasteur pipette was used to draw up and transfer the liquid, the liquid in the recesses that was being drawn up and transferred at the same time was As a result, there was a drawback that the dilution efficiency was poor.

[Means for solving problems]

Therefore, the present inventor solved this conventional drawback by making the bottom of the recess a concave shape, and the present invention is characterized by providing a plurality of recesses each having a concave bottom. This is a microbody cleaning plate.

〔Example〕

The present invention will be further explained below with reference to drawings showing one embodiment thereof.

Reference numeral 1 denotes a plate body in which a plurality of recesses 3 each having a concave bottom portion 2 are provided. The material of this plate body 1 does not matter, but
For example, synthetic resins such as polystyrene and polyvinyl chloride are preferred in terms of moldability and strength, and it is advantageous for the plate body 1 itself to be transparent for observation using the microscope 8. It is preferable that the bottom part 2 has a concave shape such that the central part thereof is the deepest part, since this allows the microscopic objects 5 such as eggs to gather in the central part. The specific size of the recess 3 is appropriately selected depending on the type of microscopic object, its purpose, etc., but it is usually 20 to 40 mm in diameter.
mm, and the deepest part is about 5 to 10 mm, and the number of them is also selected depending on the purpose and the number of times of washing, but usually about 2 to 12 is suitable.

In addition, 4 is a lid body which is placed on the plate body 1. By covering the lid body 4, it is possible to prevent the liquid 6 containing the microscopic objects 5 such as eggs from drying out, so that better results can be obtained.

[Effect]

Since the present invention is constructed as described above, when the microscopic objects 5 to be cleaned, such as eggs, are placed in the recess 3, they naturally gather in one place, so that the suction and transfer operation using the Pasteur pipette 7 is extremely easy. be able to.

Incidentally, if the cleaning method using the plate of the present invention is explained according to FIG. Place an appropriate number of microscopic objects 5 such as eggs to be washed into the recess 3a, (3) aggregate the microscopic objects 5 in the center of the field of view under the microscope 8, and (4) collect as few of the aggregated microscopic objects 5 as possible. The liquid 6 is sucked up with the Pasteur pipette 7 and transferred to the adjacent second recess 3b. Afterwards, by repeating this transfer operation three to four times using other recesses 3 in sequence, the microscopic particles are removed. Each time the particles 5 are transferred to the next recess 3, the impurities transferred together with the particles 5 are sequentially diluted and the particles 5 are cleaned.

[Effect of idea]

Therefore, according to the present invention, the microscopic objects 5 to be cleaned can be collected in one place in the recess 3, so that when the Pasteur pipette 7 performs a suction transfer operation, the microscopic objects 5 are sucked up and transferred together with the microscopic objects 5. The amount of liquid (impurities) used can be reduced to an extremely small amount, and an extremely efficient dilution cleaning effect can be obtained.

[Brief explanation of the drawing]

The drawings show an embodiment of the present invention. Figure 1 is an exploded perspective view of the lid and the plate body, Figure 2 is a cross-sectional explanatory view of the plate body, and Figures 3 (1) to (4) show the cleaning process. It is an explanatory diagram.

Claims (1)

[Scope of utility model registration request] A microbody cleaning plate characterized by having a plurality of recesses each having a concave bottom.