JPH0416126A - Vessel culture of fomes japonicus and vessel therefor - Google Patents

Abstract

PURPOSE:To improve yield by laying down a vessel for culture of Fomes japonicus put on with a cap having a seeding opening and culturing a germ of Fomes japonicus using water stored in the cap. CONSTITUTION:A vessel for culture of Fomes japonicus is prepared by covering an opening part 4 of a cup-shaped vessel body (A) containing a culture medium 1 with a film 5 having a germ opening 2 smaller than the opening part 4, putting a cap (B) having a seeding opening 3 at the position corresponding to the germ opening 4 on the opening part 4 and putting a plug (C) in the seeding opening 3. An inoculum of Fomes japonicus is then seeded on the seeding opening 3 of the vessel and cultured to spread mycellia in the culture medium 1. The vessel is subsequently laid down and water is stored in the cap (B). A germ of Fomes japonicus coming out from the germ opening 2 is cultured under a suitable humidity condition using the above-mentioned stored water.

Description

DETAILED DESCRIPTION OF THE INVENTION [Industrial Field of Application] The present invention relates to a method for cultivating a stone mushroom container and a container for culturing stone mushrooms.

More specifically, the present invention makes it possible to obtain a high yield of Cinderella mushrooms without suffering any growth inhibition due to drying when the young shoots of C. monocytogenes sprout from a medium infested with hyphae. The present invention relates to a method for cultivating a stone mushroom container and a culture container used for the container culture method.

[Conventional technology and its issues]

The present inventor has completed the following invention and has previously filed a patent application ( Special application 1986-
144437), the container is constructed by fitting a cap C into the inoculation opening 3 of the container body A and sealing the mushroom opening 2 provided on the bottom wall of the container body A with a lid 13. Mushroom mycelium is spread in medium 1 in main body A, and then,
The container main body A is inverted or tilted so that the mushroom opening 2 is at a higher position than the inoculation opening 3, the inoculation opening 3 faces the water storage surface, and the lid material 13 is removed. This is a method for culturing mushrooms in containers (see FIGS. 5 and 6), which involves the technical means of growing mushrooms (hereinafter simply referred to as the "inverted method").

[Problem to be solved by the invention]

Since the young buds of Cinnamon mushroom are sensitive to dryness, if the young buds of Cinnamon mushroom are left in a dry state, growth is often inhibited, and as a result, it is not possible to obtain a good yield of Cinnamon mushroom. Therefore, in order to prevent such a result from occurring, it is necessary to perform watering treatment at the stage when the young buds of the stone mushroom sprout.

By the way, in the case of cultivating Cinderella mushrooms using the above-mentioned inverted method, the water storage surface will be located on the opposite side from the mushroom opening 2, so in order to prevent the young shoots of Clover mushrooms from becoming dry, it is necessary to , This inverted method also requires watering treatment.

However, since medium 1, which is infested with mycelia, rarely absorbs the water that is sprinkled on it, if a large amount of water is applied at once,
Water will accumulate at the top of medium 1. As a result, young mushroom shoots sometimes rot.

Therefore, in the above-mentioned watering treatment, it was necessary to strictly determine the amount and time of watering so that the young shoots of the mushrooms would not become dry or soaked in water.

[Means to solve problems]

As a result of various studies aimed at solving the problems with the above-mentioned inverted method, the present inventor has completed a method for cultivating C. aureus in a container that solves the problems, and a container for cultivating C. aureus that is suitable for this method. be.

That is, the method for cultivating a rock mushroom container according to the present invention can be called a horizontal method when compared to the above-mentioned inverted method, in which the container body A filled with the culture medium 1 is placed on the surface forming the mushroom opening 2. A container for cultivating stone mushrooms fitted with a cap B having an inoculation port 3 consisting of a partial opening at a position corresponding to the port 2 is laid down on its side, water is stored in the cap B, and this stored water is used to incubate the mushrooms. This device is characterized in that the young shoots of the stone mushroom emerging from the mouth 2 are cultured under suitable humidity conditions.

The method for cultivating stone mushrooms in containers according to the present invention will be explained in detail.

When carrying out the method for cultivating stone mushrooms in a container according to the present invention, first, the container body A is filled with the medium 1. Medium 1
As a medium, it is possible to use a medium used in conventional stone mushroom container culture methods, such as a sawdust/rice bran medium or a medium in which wood chips are mixed therein.

Next, a cap B having an inoculation opening 3 consisting of a partial opening at a position corresponding to the mushroom opening 2 is fitted to the surface forming the mushroom opening 2 of the container main body A filled with the medium 1 for culturing the mountain ganja mushroom. Before turning the container for culture on its side, it is sterilized according to a conventional method, and the inoculum of C. chinensis is inoculated through the inoculation opening 3. Thereafter, a sterile ventilation cap C, which will be described later, is fitted into the inoculation port 3, and the inoculum is cultured according to a conventional method, and the hyphae are spread in the medium 1. Usually, the container for cultivating C. chinensis is kept upright until this stage, and then it is laid down, but it is also possible to keep the container for cultivating C. chinensis on its side after inoculation with the inoculum.

When the mycelium reaches the stage where it has spread, after removing the palate C,
Water is stored in cap B. If the mushrooms are cultured in this state for about 7 days, young sprouts of the rock mushroom will emerge from the mushroom opening 2.

The young shoots of this mushroom grow under suitable humidity conditions, using stored water.

Next, the structure of the container for cultivating C. chinensis according to the present invention will be explained in detail based on the drawings.

The first aspect of the container for cultivating C. chinensis according to the present invention is as follows:
An opening 4 of a cup-shaped container main body A is covered with a film 5 which is smaller than the opening 4 and has a mushroom opening 2, and has a partial opening at a position corresponding to the mushroom opening 2. A cap B having an inoculation opening 3 is fitted into the opening 4 of the container body A with the end of the film 5 interposed therebetween, and the inoculation opening 3 is inserted into the opening 4 of the container body A.
It is characterized in that a palate C having aseptic breathability is fitted into the mouth.

The container main body A has a cup shape, and the culture medium 1 is filled therein. Then, the opening 4 of the container body A is covered with the film 5. This film 5 is provided with a mushroom opening 2 that is smaller than the opening 4. The reason why the mushroom opening 2 is smaller than the opening 4 is to prevent the inoculum inoculated into the mushroom opening 2. This is to spread the virus continuously in a radial manner from that point.

The diameter of the mushroom opening 2 is preferably about 14 to 18 inches. The mushroom opening 2 may be provided in the film 5 before or after the opening 4 is covered with the film 5.

In the latter case, for example, using a conical plastic aid (not shown) with a hole leading from the tip to the back end,
The mushroom opening 2 may be formed by piercing the film 5. If this auxiliary tool is left in place until the time of inoculation with the inoculum, this auxiliary tool can also function as a guide tube for the inoculum.

The material of the container body A is not particularly limited, and may be made of plastic or glass as long as it can withstand heat treatment during sterilization. In view of ease of handling, plastic, particularly polypropylene, is preferred. Further, the material of the film 5 may be plastic or nonwoven paper.

The cap B is provided with an inoculation port 3 consisting of a partial opening at a position corresponding to the mushroom inoculation port 2 provided in the film 5. The reason why the inoculation port 3 is not completely opened but is partially opened is to prevent bacterial contamination and to form a water storage part within the cap B. The cap B is fitted into the opening 4 of the container body A via the film 5. Therefore, the end of the film 5 will be sandwiched between the container body A and the cap B. In the drawing, the shape of the cap B is funnel-shaped, but it is not necessarily limited to this shape, but it must not have a shape that causes bacterial contamination. You can select and use it according to the main body A.

A palate C having sterile air permeability is fitted into the inoculation port 3 of the cap B from inoculation with the inoculum until the hyphae spread. This palate C has the function of preventing bacterial contamination from the inoculation port 3 and providing ventilation to the culture medium 1, and is fitted on the outside or inside of the inoculation port 3. An example of the former is shown in FIG. 3, while a cotton plug conventionally used in container culture methods for mushrooms is suitable as an example of the latter (see FIG. 1).

Next, in the second aspect of the container for cultivating stone mushrooms according to the present invention, the opening 4 of the cup-shaped container main body A is
A plate 5'' provided with a smaller mushroom opening 2 is fitted inside, and a cap B having an inoculation opening 3 consisting of a partial opening at a position corresponding to the mushroom opening 2 is fitted on the outside, 3 is characterized by a palate C having aseptic ventilation fitted therein.

The difference from the first embodiment is that a plate 5' is used instead of the film 5. Mushroom opening 2 in the center of board 5'
is provided. The outer circumference of the plate 5'' has approximately the same diameter as the opening 4 of the container body A, and the plate 5° has the same diameter as the opening 4 of the container body A.
mated to. In the drawing, this plate 5 is the cap B.
It is in contact with the lower end of a protrusion 6 provided on the inner wall of. This is because the internal pressure of medium 1 increases due to the spread of hyphae, and plate 5
It acts to suppress the force that lifts the temperature. In addition, board 5
Multiple small holes 7 (about 3 in diameter
If a pore (about the size of a trough) is provided, the medium 1 in which hyphae are spread will exchange gas through these small holes 7, so the growth power of the hyphae will increase, and it is possible to obtain a higher yield of stone mushrooms. Even if water is stored in the cap B, since the medium I is already infested with mycelia, water will not leak into the medium 1 through the small holes 7. 8 in the figure indicates a wall provided between the mushroom opening 2 and the small hole 7. This wall 8 has the function of preventing the inoculum from scattering when inoculating the inoculum of Cinnamon mushroom from the inoculation port 3.

In order to ensure that the container body A and cap B fit together,
In the embodiment shown in FIG. 3, a retaining projection 9 is provided on the outside of the container body A, and an engaging recess 10 is provided on the inside of the cap B. Aspects other than this, for example, container body A
and the cap B may be engaged with each other by screwing.

The palate C is partially constituted by a molded body having a gap 11. This MC is provided with aseptic ventilation through the gap 11.

Next, the third aspect of the container for culturing mushrooms according to the present invention is that a flexible container body A provided with a mushroom opening 2 is provided with a partial opening at a position corresponding to the mushroom opening 2. The device is characterized in that a cap B having an inoculation opening 3 of the type shown in FIG.

The feature of this embodiment is that the flexible container body A
The point is to use After sterilizing the flexible container main body A, ie, the bag, filled with the medium 1, the inoculum is inoculated through the opening 12, the opening 12 is closed, the inoculum is cultured, and the medium 1 is spread with hyphae. Thereafter, the cap B is fitted onto the bottom side of the bag, and a hole is made in the bottom of the bag to form the mushroom opening 2 using the aforementioned auxiliary tool (not shown). In addition, since mycelia are spread inside the bag and the bag retains its shape, water will not leak even if water is stored in the cap B after the cap B is fitted onto the bottom side of the bag.

〔Example〕

Next, one embodiment of the present invention will be described.

Example 1 50 polypropylene culture containers for rock mushrooms having the structure shown in FIG. .

Next, after covering the opening 4 of the container body A with a polypropylene film, the cap B was fitted so as to sandwich the ends of the polypropylene film.

Next, the polypropylene film was pierced using a conical polypropylene auxiliary tool having a hole extending from the tip to the rear end to form the mushroom opening 2. After sterilizing each container for cultivating C. chinensis, each cap B
The mycelium of C. chinensis was inoculated through inoculation port 3 and cultured for 25 days. After waiting for the mycelium to spread into the medium 1, each container for cultivating C. chinensis was turned on its side, and about 60 d of water was stored in each cap B (see FIG. 2). When the mushrooms were cultured in this state, young buds of the rock mushroom sprouted at the mushroom opening 2 on the 7th day. Subsequently, the culture was continued for 25 days, and the mushrooms were generated from the mushroom opening 2.

The dry weight of the obtained Cinderella mushrooms was 30 g on average per container for cultivating C. monocytogenes.

Example 2 The same procedure as in Example 1 was carried out except that a polypropylene mushroom cultivation container having the structure shown in FIG. 3 was used.

The average dry weight of the obtained Hemlock mushrooms was 32 g per container for culturing Hemlock mushrooms.

Comparative Example 1 The procedure of Example 1 was followed except that a polypropylene mushroom culture container having the structure shown in FIG. 5 was used and an inverted method was used (see FIG. 6). occurred.

The average dry weight of the obtained stone mushrooms was 10 g per mushroom culture container.

[Effects] According to the method for cultivating a stone mushroom container and the container for culturing stone mushrooms according to the present invention, since the water storage portion is located near the location where young shoots of stone mushrooms are generated, the young shoots of stone mushrooms do not fall into a dry state.

Furthermore, since a large amount of water is constantly emitted from the water storage section, there is no need to perform water sprinkling treatment that takes into account the amount and time of watering. moreover,
Since it is possible to culture the Stone Mountains by turning the vessels for culturing the Mountains' Mountains sideways, it is possible to stack the vessels for culturing the Mountains' Mountains, and it is possible to increase the yield of Stone Mountains per unit area. Therefore, according to the method for cultivating a stonecrop in a container and the container for culturing the stonecrop according to the present invention, the stonecrop can be obtained in good yield.

[Brief explanation of the drawing]

FIG. 1 is a perspective view of a center longitudinal cross-section of an embodiment of the container for cultivating C. chinensis according to the present invention, FIG. FIG. 4 is a central vertical cross-sectional perspective view of another embodiment of the stone mushroom culture container according to the present invention, and FIG. The figure shows a central vertical sectional view of one embodiment of the mushroom cultivation container used in the inverted method, and FIG. 6 shows a central vertical sectional view of the mushroom cultivation container of FIG. 5 in a used state. In the figure A-1 main body, B, - cap, C- palate, ■
- trail, 2- mushroom opening, 3- inoculation opening, 4- opening, 5- film, 5''- plate.

Claims (4)

[Claims] (1) A stone mushroom in which a cap B having an inoculation port 3 consisting of a partial opening at a position corresponding to the mushroom opening 2 is fitted to the surface forming the mushroom opening 2 of a container body A filled with a culture medium 1. The cultivation container is laid down on its side, water is stored in the cap B, and the stored water is used to cultivate young sprouts of the mountain rock mushroom emerging from the mushroom opening 2 under suitable humidity conditions. Container culture method. (2) At the opening 4 of the cup-shaped container body A, the opening 4
A film 5 provided with a smaller mushroom opening 2 is placed over the film 5, and a cap B having an inoculation opening 3 consisting of a partial opening at a position corresponding to the mushroom opening 2 is placed between the ends of the film 5. 1. A container for cultivating C. chinensis, characterized in that it is fitted into an opening 4 of a container body A, and a palate C having sterile air permeability is fitted into the inoculation port 3. (3) At the opening 4 of the cup-shaped container body A, the opening 4
A plate 5' provided with a smaller mushroom opening 2 is fitted inside, and a cap B having an inoculation opening 3 consisting of a partial opening at a position corresponding to the mushroom opening 2 is fitted on the outside, 3. A container for cultivating C. chinensis, characterized in that a palate C having aseptic ventilation is fitted into the container. (4) A cap B having an inoculation port 3 consisting of a partial opening at a position corresponding to the mushroom inoculation port 2 is fitted onto a flexible container body A provided with the mushroom inoculation port 2, and the inoculation port 2 is 1. A container for cultivating C. chinensis, characterized in that a palate C having sterile air permeability is fitted into the opening 3.