JPH04200534A - Instrument for measuring physiological function of lung - Google Patents

Abstract

PURPOSE:To eliminate the fluctuations in experimental data by measuring persons and to obtain the exact data by automaticall controlling the degrees of the pressurization and pressure reduction in an animal box and the time required for the same as well as the time before an atm. pressure is restored from a reduced pressure state by a sequencer. CONSTITUTION:A 1st solenoid valve 20 is opened by the instruction of a sequencer 24 and a pressure reducing pump 17 is operated to reduce the pressure in the animal box 2. A 2nd solenoid valve 21 and a 3rd solenoid valve 22 are held closed and a pressurizing pump 18 is held stopped at this time. The atm. pressure is then restored in the animal box 2 by closing the 1st solenoid valve 20, stopping the pressure reducing pump 17 and opening a 3rd solenoid valve 22. The inside of the animal box 2 is pressurized by closing the 3rd solenoid valve 22, opening the 2nd solenoid valve 21 and operating the pressurizing pump 18. A dosing drug is evaluated by determining the above-mentioned operations as one cycle. Finally, the atm. pressure is restored in the animal box 2 by closing the 2nd solenoid valve 21, stopping the pressurizing pump 18 and opening the 3rd solenoid valve 22 and, thereafter, the 3rd solenoid valve 22 is closed to return to the first.

Description

DETAILED DESCRIPTION OF THE INVENTION [Industrial Field of Application] This invention relates to an apparatus for determining pulmonary physiological function used in animal experiments necessary in the development of pharmaceutical products.

[Prior Art] When developing a therapeutic drug for pulmonary emphysema, it is necessary to conduct experiments using experimental animals such as hamsters using lung physiological function as an index to evaluate the drug's efficacy.

In this experiment, an apparatus as shown in FIG. 2 was conventionally used. The conventional lung physiological function measuring device (1') is provided with an animal box (2) for containing the experimental animal (A), and the experimental animal (A) is placed inside the animal box (2).
A tracheal catheter (3) for insertion into the trachea of the animal and an esophageal catheter (4) for insertion into the esophagus are each airtightly inserted from outside the animal box (2).

An airflow resistance tube (5) is connected to the tracheal catheter (3), and the airflow resistance tube (5) is connected to the first differential pressure detector (6).
, the recorder (9) via the amplifier (7) and the integrator (8)
It is connected to the.

In addition, the tracheal catheter (3) is branched to detect a second differential pressure!
! (10) is also connected.

The air flow resistance tube (5) converts the air flow rate into a pressure difference, the first differential pressure detector (6) converts the pressure difference into an electrical signal, and the integrator (8) converts the air flow into a pressure difference. It converts the flow rate into air volume by integrating it.

The esophageal catheter (4) is connected to a second differential pressure detector (10) and connected to a recorder (9) via an amplifier (11).

The second differential pressure detector (10) converts the pressure difference between the esophagus and the trachea (hereinafter referred to as esophageal pressure) into an electrical signal.

A tube (12) is airtightly connected to the animal box (2), and the tube (12) is connected to an aspirator (14) and a Douglas bag (15) through a three-way cock (13). ing. The aspirator (14) is used to reduce the pressure inside the animal container, and the Douglas bag (15) is a rubber bag that contains air. This is to pressurize the inside of the box (2).It is also used to pressurize the inside of the box (2).
A pressure gauge (16) for measuring the internal pressure is airtightly connected to the .

In this experiment, after administering the drug to be evaluated, the experimental animal (A), which has been rendered asphyxic by anesthesia and unable to breathe spontaneously, is placed in an animal box (2), and the trachea and esophagus of the experimental animal (A) are removed. Insert the tracheal catheter (3) and esophageal catheter (4) into
4), reduce the pressure inside the animal box (2) to the specified pressure while watching the pressure gauge (16), then release the reduced pressure, and when the inside of the animal box (2) returns to normal pressure, Operate the three-way cock (13) and press the Douglas bag (15) to pressurize the inside of the animal box (2) to a predetermined pressure.

The above operation is considered as one cycle, and the esophageal pressure and air volume of the experimental animal are represented in a graph on the recorder (9).

When the pressure inside the animal box (2) is reduced, the airflow resistance tube (5)
> through the tracheal catheter (3), the lungs of the experimental animal (A) expand. When the reduced pressure is released and the pressure is returned to normal, the lungs of the experimental animal (A) return to their original state.

When the inside of the animal box (2) is pressurized, air flows out through the airflow resistance tube (5), causing the lungs of the experimental animal (A>) to contract and collapse.

In this experiment, animals with emphysema lose their lung elasticity, so the change in air volume during pressurization and depressurization is greater than in normal animals. Therefore, in this experiment, using experimental animals in which emphysema was prevented by administering elastase directly into the trachea, it is possible to evaluate therapeutic agents for pulmonary emphysema using changes in air volume during pressurization and depressurization as an indicator.

[Problems to be solved by the invention] In conventional devices, the pressure inside the animal box was adjusted by the operator manually switching the three-way cock while looking at the pressure gauge. Not only that, but there were also cases where variations occurred in the experimental data even for the same measurement person.

[Means for Solving the Problems] The present invention has been developed in view of the above-mentioned points, and it is an object of the present invention to provide a lung physiological function measuring device that does not cause variations in experimental data depending on the measurer.

In the pulmonary physiological function measuring device of the present invention, a catheter to be inserted into the trachea of a laboratory animal and a catheter to be inserted into the esophagus are each airtightly connected in an animal box, and an airflow resistance tube is connected to the tracheal catheter. 1 fc is out,
The airflow resistance tube is connected to a recorder via a first differential pressure detector and an integrator, and the tracheal catheter is also branched and connected to a second differential pressure detector, and the tracheal catheter is connected to a second differential pressure detector via a first differential pressure detector and an integrator. In the pulmonary physiological function measurement apparatus, the catheter is connected to the second differential pressure detector, and the second differential pressure detector is connected to the recorder. The pressurizing pump is airtightly connected to the second IE magnet, and the displacement adjustment device is airtightly connected to the third electromagnetic valve via the first, second, and third electromagnetic valves. The opening and closing of the pump and the operation and stopping of the decompression pump and pressurization pump are regulated by a sequencer.

[Example 1] FIG. 1 shows a schematic configuration diagram of a pulmonary physiological function measuring device (1) of the present invention.

The pulmonary physiological function measuring device <1) of the present invention includes an animal box (2), a tracheal catheter (3), an esophageal catheter (4), an airflow resistance tube <5), a first differential pressure detector (6),
The second differential pressure detector (10), amplifiers (7) (11), integrator (8), recorder (9) and pressure gauge (16) are the same as those of the conventional ones.

In the pulmonary physiological function measuring device (1) of the present invention, the decompression pump (17) connects the first tm valve (20) with the pressurization pump (18).
) connects the second solenoid valve (21>) to the displacement adjustment device (19).
are connected to the pipes (23) through the third solenoid valves (22), respectively.
through which it is hermetically connected to the animal box (2).

The displacement adjustment device (19) is used to return the pressure inside the animal box (2) to normal pressure by opening the third solenoid valve (22>), and the speed at which the pressure is returned to normal pressure is controlled by a needle valve (not shown). regulated by a first solenoid valve (20);
The opening and closing of the second solenoid valve (21) and the third solenoid valve (22>) are each regulated by a sequencer (24).The sequencer also operates and stops the pressure reduction pump (17) and pressure pump (18). (24).The broken lines in Fig. 1 indicate the flow from the sequencer (24) to the magnetic valves (20) (21) (22) and the pressure reducing pump (17).
and electrical wiring to the pressure pump (18).

Next, the operation of the lung physiology and function measuring device (1) of the present invention will be explained.

As with the conventional device, after administering the drug to be evaluated, the anesthetized experimental animal (A>) is placed in the animal box (2
) and insert the tracheal catheter (3) and esophageal catheter (4) into the experimental animal (A). Tracheal catheter (
3) is connected to the air flow resistance tube (5), and the air flow resistance tube (5) is connected to the first differential pressure detector (6) and the amplifier (7).
> and an integrator (8>) to the recorder (9).The tracheal catheter (3) is also branched off and connected to a second differential pressure detector (10).The esophageal catheter (4
) can be connected to the second differential pressure detector (10), and the amplifier (
11) and is connected to the recorder (9). These are the same as conventional devices.

To change the pressure inside the animal box (2), first turn on the first solenoid valve (20) according to the instructions from the controller (24).
) to enable the decompression pump (17) to operate, reducing the pressure inside the animal box (2).At this time, open the second solenoid valve (2).
1) and the third solenoid valve (22) are closed, and the pressurizing pump (18) is stopped.

Next, close the first solenoid valve (20) and close the pressure reducing pump (17).
and open the third solenoid valve (22) to return the inside of the animal pond (2) to normal pressure.

Next, the third solenoid valve (22) is closed, and the second solenoid valve (22) is closed.
21), enable the pressurization pump (18) to operate, and pressurize the inside of the animal box (2). The above operations are considered as one cycle. The esophageal pressure and air volume of the experimental animal (A>) are measured, and the recorder (9) is opened. ) to evaluate the administered drug.

Finally, close the second solenoid valve (21) and pressurize the pump (1).
8), open the third t'ai valve (22) to return the inside of the animal box (2) to normal pressure, close the third 'I solenoid valve (22), and then open the third t'ai valve (22) to return the inside of the animal box (2) to normal pressure. Repeat the experiment using The degree of pressurization and depressurization, the time required for it, and the time it takes to return to normal pressure from a depressurized state are determined by the controller (
24) can be arbitrarily set.

[Effects] According to the pulmonary physiological function measuring device of the present invention, the degree of pressurization and depressurization in the animal box, the time required for it, and the time from the depressurized state to the normal pressure can be automatically adjusted by the sequencer. Therefore, there is no variation in experimental data depending on the measurer, and accurate data can be obtained.

[Brief explanation of the drawing]

FIG. 1 is a schematic diagram of the pulmonary physiological function measuring device of the present invention;
The second section shows a schematic diagram of a conventional pulmonary physiological function measuring device. (1) Pulmonary physiological function measuring device (2) Animal box (3) Tracheal catheter (4) Esophageal catheter (5) Airflow resistance tube (6) First differential pressure detector (9> Recorder (10) )...Second differential pressure detector (1
7)...Reduction pump (18)...Pressure bomb (19)
... Displacement adjustment device (20) ... First solenoid valve (2
1)...Second solenoid valve (22)...Third solenoid valve <24)...Sequencer! ! pH chart

Claims (1)

[Claims] A catheter for insertion into the trachea and a catheter for insertion into the esophagus of the experimental animal are each airtightly connected in the animal box, and an airflow resistance tube is connected to the tracheal catheter, and the airflow resistance tube is connected to a first The tracheal catheter is connected to a recorder via a differential pressure detector and an integrator, and the tracheal catheter is also branched and connected to a second differential pressure detector, and the esophageal catheter is connected to the second differential pressure detector. In a pulmonary physiological function measuring device connected to a detector, and the second differential pressure detector is connected to the recorder, a decompression pump is connected to the animal box via a first electromagnetic valve, and a pressurization pump is connected to the animal box. Through the second solenoid valve, the displacement adjustment device is connected airtightly through the third solenoid valve, and the opening and closing of the first, second and third solenoid valves, and the pressure reduction pump and pressurization are controlled. A pulmonary physiological function measuring device characterized in that activation and stopping of a pump is regulated by a sequencer.