JPH0438466A - Apparatus for measuring blood coagulation - Google Patents

Abstract

PURPOSE:To shorten the time required in the analysis of blood coagulation by moving at least one of a specimen cassette and luminous flux in the series arranging direction of blood specimens. CONSTITUTION:A drive apparatus 28 moves at least one of a specimen cassette 20 and an optical mechanism constituted of a light emitting element 11 and a photodetector 12 in the series arranging directions of blood specimens under the control of a microprocessor 26 to successively measure the changes of the absorbancies of the blood specimens arranged in series. The luminous flux of the light emitting element 11 successively transmits through the blood specimens to be inputted to a luminous intensity calculation apparatus 27 and the absorbancies of the blood specimens are successively recorded at every specimens 13 - 19 in which various reagents are distributed to be stored in a memory 36 through a register 35.

Description

[Detailed Description of the Invention] [Industrial Application Field] The present invention relates to a blood coagulation measuring device that analyzes the blood coagulation process by measuring the amount of change in absorbance of a blood sample, and particularly relates to a blood coagulation measuring device that analyzes the blood coagulation process by measuring the amount of change in absorbance of a blood sample. The present invention relates to a blood coagulation measuring device that can be used to measure blood coagulation.

(Technical background) It is well known that when blood exits blood vessels, it forms a blood clot and coagulates, and that this process takes different forms and times depending on various diseases.

For example, fifurinogensis does not allow fitulin threads to form, and thrombocytopenia does not allow fitulin threads to bind to the clusters. In addition, platelets contain many factors related to blood clotting, and clotting time is influenced by these factors.
Even with the same blood sample, it is necessary to perform measurements using multiple types of reagents in order to analyze the blood flow process in detail.

[Prior art]

Conventional blood coagulation measurement devices that analyze the process of blood coagulation include those that insert a detector into a blood sample to detect signals, and those that measure changes in fluidity by rotating a sample container. However, the problem is that signs of coagulation occur earlier than the actual coagulation time due to the contact effects of blood coagulation factors present in the blood, and the measurement results obtained by highly skilled techniques are not correlated. Therefore, there has been a strong demand for a device that allows blood samples to stand still to eliminate the effects of contact with coagulation factors.

Fig. 583 shows a conventional blood coagulation measuring device of the type in which such a blood sample is left still.
It is equipped with a light emitting device 2 that emits a luminous flux 1 (nm) and a light receiving element 3 such as a phototire autograph that receives this luminous flux 1, and a sample container 4 containing blood to be analyzed is placed on the luminous flux 1 for measurement. is configured to do so. In addition, the code 5 in the figure is A
6 is a microprocessor that measures the amount of change in absorbance of the blood sample based on the output of the A/D converter, 7 is a display device that displays the measured values, and 8 is a printer device that records the measurement results. It is.

Figure 4 shows an example of the amount of change in absorbance of a blood sample.
The measurement starts when the reagent is added, and after the time tol'j (
For example, absorbance A after 5 seconds). or measured. moreover,
Measurement is continued until the absorbance reaches rAo+ΔAJ (ΔA is the value input from the keyboard, 1, for example, ΔA=9.100), and the time t (seconds) at this time is measured. Then, the concentration C is calculated from the standard curve C=f (t) from this t value.
Calculated based on.

Furthermore, the absorbance A1 is measured after a pre-specified time 1+ (for example, 90 seconds), and the fibrinoken concentration is determined from the calculated absorbance difference F A I - Ao J. PT value measurement (value, normal value, abnormally high value) - General -C.

[Invention or problem to be solved]

By the way, as mentioned above, the blood coagulation process exhibits various aspects depending on the medical condition and the like, and therefore, it is usually necessary to analyze multiple items even for human blood.

However, in such conventional blood coagulation measuring devices, the sample container must be left standing until the measurement of the container-item is completed, and it takes a long time to obtain analysis results for multiple items. I need. In order to solve this problem, the only way to solve this problem is to shorten the time by lining up multiple identical measurement devices or installing multiple optical devices in parallel, which would be costly. The problem is that the size of the device is enormous, and the installation space is also large.

The present invention was devised in view of the current situation, and its purpose is to reduce the cost and amount of loaded M'lFt as much as possible, and to reduce the time required for blood coagulation analysis involving multiple items. The purpose of the present invention is to provide a blood coagulation measuring device that can significantly shorten the time required.

[Means to solve the problem]

In order to achieve the above object, the present invention captures light irradiated from a light emitting element through a blood sample with a light receiving element, and measures the change in absorbance of the blood sample to determine the coagulation state of the blood sample. A blood coagulation measuring device configured to measure blood coagulation is provided, a sample cassette is provided in which a plurality of blood samples are arranged in series, and at least one of the sample cassette and the light beam is directed in the direction in which the blood samples are arranged in series. This feature is characterized in that it is configured by providing a relative moving means for moving.

[Effect]

Therefore, in the blood coagulation measuring device according to the present invention,
Multiple blood samples are sequentially irradiated with a light beam, and the light falling on each blood sample is sequentially captured by a light receiving element and flows through the absorbance changing lid, significantly reducing the time required for blood coagulation analysis involving multiple items. It is something that we are trying to change.

(Embodiment) Hereinafter, the present invention will be described in detail based on an embodiment shown in the accompanying drawings.

10th shows a blood coagulation measuring device 10 according to this embodiment.

This blood coagulation measuring device 10 includes a light emitting element 11 that generates light of a specific wavelength, a light receiving element 12 that receives the light and generates an electrical signal, and a plurality of (for example, seven) light receiving elements containing a patient's blood sample. ) and a sample force set 20 for arranging the plurality of sample containers 13 to 19 in series.

For example, as shown in FIG. 2, this sample cassette 20 has a storage hole 21 that can store a plurality of transparent sample containers 13 in series. Round holes 22 penetrating in the direction are respectively opened, and the light beam irradiated from one light-emitting element 11 passes through the round holes 22, the transparent sample container 13, and the blood sample mixed with the reagent, and reaches the light-receiving element 12. is input.

Further, reference number 25 in FIG. 1 is an A/D converter that amplifies and digitally converts the output of the light receiving element 12, 26 is a microprocessor device, and 27 is a device that measures the amount of change in absorbance based on the signal from the light receiving element 12. The figure shows a luminous intensity calculation device for.

On the other hand, the reference numeral 28 in the figure is a drive device that can highly control the amount of drive, such as a stepping motor.

This drive device 28 starts a gear mechanism or the like to move at least one of the sample cassette 20 or the optical mechanism composed of the light emitting element 11 and the light receiving element 12 in the direction shown by the arrow in the figure (the direction in which the blood samples are arranged in series). It is something that makes you

In addition, since this invention sequentially measures the absorbance changes of blood samples arranged in series, the sample cassette 2
The change in absorbance may be sequentially measured by moving the optical system 30, or the change in absorbance may be sequentially measured by moving the optical system 30.

In this case, since the reaction between the reagent and the blood sample is progressing in the sample containers 13 to 19, the reagent and blood sample in the sample containers 13 to 19 should be moved more quietly so that the reagent and blood sample in the sample containers 13 to 19 are not agitated by vibration due to the above-mentioned movement. Moreover, it is important to move at a slow speed, and from this point of view, a method of moving the optical system 30 is more suitable.

This movement of the specimen force 20 or the optical system 30 is measured via the sensor 29. Although this sensor 29 is shown placed on the right side of the optical system 30 in FIG. 1, it is actually placed at the same position as the optical system 30, and is configured to obtain a synchronization signal. .

Further, reference numeral 31 in the figure denotes an A/D converter 32 that amplifies and digitally converts the output signal from this sensor 29.
33 is a D/A converter that converts the output of the drive control device 32 into analog, and 34 is the drive control device that calculates the operating amount of the drive amount M28 based on the output of the sensor 29; Specimen containers 13 to 1 during processing
a number identification device for identifying a unique designation number (ID) of 9;
5 is the output data of the number identification device 34 and the luminous intensity calculation device 1
27 is a register that sequentially takes in the absorbance of the blood sample numbered based on the output, and 36 is a memory device 2 that sequentially stores the register output. Note that 37 is a display device that outputs the absorbance data stored in the memory device 36;
8 is a printer device that prints out the absorbance data.

By the way, the blood coagulation measuring device 1O according to this embodiment is one that moves the sample cassette 20 or the optical system 30, and directs light generated from the light emitting element 11 to the sample cassette 2.
0 round hole 22 for a certain period of time (for example, 0.1 to 1 sec)
It is important to control the positioning of the sample cassette 20 or the optical system 30 because it is necessary to measure the absorbance through the sensor 29. uses an encoder (regardless of incremental or absolute), and the drive amount W1
It is possible to use a stepping motor for 28. In this case, the drive control device 32 that calculates the drive amount,
The output signal from the sensor 29 is output to the drive device 112B.

On the other hand, the drive amount $28 by which the signal is input moves the sample cassette 20 or the optical system 30 at a constant speed.

As a result, the light flux (broken line arrow in the figure) passes through the blood sample sequentially and is input to the light intensity calculation device 27, and the absorbance of each sample 13 to 19 into which various reagents have been dispensed is sequentially recorded and transmitted via the register 35. It is stored in the memory device I36.

Then, when the light beam reaches the position of the last blood sample 19,
After the absorbance has been measured, the drive device 28 is driven in reverse to measure the absorbance of the samples 19, 18, 17, . . . 13 in the order of 13, and the absorbance of each blood sample is recorded in a line graph .

Note that the wavelength of the light beam generated by the light emitting element 11 is not particularly limited to a certain wavelength (for example, 340 nm),
Light of other wavelengths can be irradiated depending on the measurement item. In this case, if a multi-wavelength photometer using a grating mirror or the like is used as a light receiving device, even more precise analytical results can be obtained.

(Effects of the Invention) As explained above, according to the blood coagulation measuring device according to the present invention, means for moving at least one of the sample cassette and the optical system device in the direction in which the blood samples are arranged in series is provided. Therefore, even when measuring multiple items on the same sample, the measurement time can be significantly shortened compared to conventional techniques, and the cost and equipment capacity are also significantly lower than conventional devices. This has the effect of reducing the

[Brief explanation of the drawing]

112i1 is a diagram showing a blood coagulation measuring device according to an embodiment of the present invention, FIG. 2 is a perspective view of a sample cassette used in the same blood coagulation measuring device, and FIG. 3 is a conventional blood coagulation measuring device. FIG. 4 is a block diagram showing an example, and a graph diagram showing an example of a gradual change in absorbance of a blood sample. [Explanation of symbols] 1O...Blood coagulation measuring device 11-...Light emitting element 12...Light receiving elements 13 to 19...Sample container 20...Sample cassette 26...Microbrossel...
Sensor 27...Light intensity calculation device 28...Drive device 29
...Sensor device 30...Optical system device 32...
Drive control device jJl 34...Number identification device Patent applicant Nitteku Co., Ltd.

Claims (1)

[Claims] In a blood coagulation measuring device configured to capture light emitted from a light-emitting element through a blood sample with a light-receiving element, and measure the coagulation state of the blood sample by measuring changes in absorbance of the blood sample. A blood coagulation measurement characterized in that a sample cassette for arranging a plurality of blood samples in series is provided, and a relative moving means is provided for moving at least one of the sample cassette and the light beam in the direction in which the blood samples are arranged in series. Device.