JPH0444080Y2

JPH0444080Y2 -

Info

Publication number: JPH0444080Y2
Application number: JP818986U
Authority: JP
Priority date: 1986-01-22
Filing date: 1986-01-22
Publication date: 1992-10-16
Also published as: JPS62153900U

Description

【考案の詳細な説明】（産業上の利用分野）本考案は細胞を培養するときに使用する細胞培
養液吐出デイスペンサーに関するものである。[Detailed Description of the Invention] (Industrial Application Field) The present invention relates to a cell culture fluid discharge dispenser used when culturing cells.

一般に細胞を培養する器として、複数個のウエ
ルを有したトレイを利用している。ウエル内での
細胞培養を正常に継続するためには、適切な時期
に培養液を交換しなければならない。この交換作
業に用いるデイスペンサーに関するものである。 Generally, a tray having a plurality of wells is used as a container for culturing cells. In order to continue cell culture in the wells normally, the culture medium must be replaced at an appropriate time. This article relates to a dispenser used for this replacement work.

（従来の技術）従来の培養液の分注作業においては、実験者自
身がピペツトを用いてウエル内の培養液の吸引お
よび新しい培養液の吐出作業を行つていた。この
際吐出作業によつてウエル内の細胞が移動しない
ように、培養液の吐出速度を遅くしたり、あるい
はウエルの壁に吐出口を接触させて吐出作業を行
つていた。またポンプを有するデイスペンサーシ
ステムで分注する場合でも、吐出口の径を小さく
したり、あるいは吐出速度小さくしたりしてい
た。しかし上記の方法においては、吐出速度を極
端に遅くしないと細胞が移動してしまう。(Prior Art) In a conventional culture solution dispensing operation, the experimenter himself used a pipette to aspirate the culture solution in the well and discharge new culture solution. At this time, in order to prevent the cells in the well from moving during the discharging operation, the discharging speed of the culture solution is slowed down, or the discharging operation is performed with the discharging port in contact with the wall of the well. Furthermore, even when dispensing with a dispenser system having a pump, the diameter of the discharge port is made small or the discharge speed is made small. However, in the above method, cells will move unless the ejection speed is extremely slowed down.

（考案が解決しようとする問題点）従来の方法で細胞が移動しないように分注する
場合、吐出速度を極端に遅くしなければならない
し、また細胞が移動して一箇所に偏つてしまうと
細胞の成長に悪影響を与える等の問題点がある。(Problems that the invention aims to solve) When dispensing to prevent cells from moving using the conventional method, the dispensing speed must be extremely slow, and if the cells move and become concentrated in one place. There are problems such as adverse effects on cell growth.

（問題点を解決するための手段）第１図は上記問題点を解決するためになされた
本考案の細胞培養液吐出デイスペンサーの正面図
で、チユーブ５の先端に培養液の吐出チツプ４を
有するデイスペンサーにおいて、吐出チツプ４か
ら下方に延びた支持部材３の先端に、円錐形部品
２が、該円錐形部品２の先端が前記吐出チツプ４
の先端に対向するように支持されている。この円
錐形部品２は、例えば、ステンレス等の金属でで
きている。同図において１は容器である。(Means for Solving the Problems) FIG. 1 is a front view of the cell culture fluid dispensing dispenser of the present invention, which was made to solve the above problems. In the dispenser, a conical part 2 is disposed at the distal end of the support member 3 extending downward from the dispensing tip 4, and the distal end of the conical part 2 is connected to the dispensing tip 4.
It is supported so as to face the tip of the This conical part 2 is made of metal such as stainless steel, for example. In the figure, 1 is a container.

第２図は本考案のデイスペンサーの斜視図であ
る。 FIG. 2 is a perspective view of the dispenser of the present invention.

（作用）培養液の吐出速度は速くても、培養液は容器へ
注入される前に円錐形部品に当たり、細胞に直接
当たらないため、ウエル底にある細胞も偏らな
い。また、円錐形部品の先端に当たつた培養液は
円錐形の底辺に向かつて円錐面に沿つて分散しな
がら拡がるため、円錐形部品の底辺から容器に落
ちる培養液は、自然落下速度で水滴となつて、し
かも容器の一箇所に偏らずに供給される。(Function) Even if the discharge speed of the culture solution is high, the culture solution hits the conical part before being injected into the container and does not directly hit the cells, so the cells at the bottom of the well are not biased. In addition, the culture solution that hits the tip of the cone-shaped part spreads toward the bottom of the cone while being dispersed along the conical surface, so the culture solution that falls from the bottom of the cone-shaped part into the container drops into water droplets at the rate of natural fall. Moreover, it is supplied evenly to one part of the container.

（実施例）円錐形部品２として、ステンレス製のものを用
い、第１図（または第２図）に示すように、吐出
チツプ４とチユーブ５の境界付近に挿入されたリ
ング状部材７に、下方に延びる２本の支持部材３
を少し開くように取り付けてなるデイスペンサー
を用い、第３図に示す様に、チユーブ５にポンプ
６をつなぎ、培養液の吸引、吐出をポンプ６で行
つた。なお、１１はウエルである。(Example) The conical part 2 is made of stainless steel, and as shown in FIG. 1 (or FIG. 2), a ring-shaped member 7 inserted near the boundary between the discharge tip 4 and the tube 5 is Two supporting members 3 extending downward
As shown in FIG. 3, a pump 6 was connected to the tube 5 using a dispenser which was attached so as to open slightly, and the pump 6 sucked in and discharged the culture solution. Note that 11 is a well.

（考案の効果）上述のとおり、本考案のデイスペンサーは極め
て簡易な構造で、特に円錐形部品を用いたことに
より培養液の吐出速度を落とすことなく、従つて
培養液の交換作業も短時間で行うことができ、し
かも細胞がウエル内で移動して偏ることがなく、
細胞の培養状態に悪影響を与えないのが大きな効
果である。(Effects of the invention) As mentioned above, the dispenser of the present invention has an extremely simple structure, and in particular, by using conical parts, the dispensing speed of the culture solution is not reduced, and therefore, the work of replacing the culture solution can be done in a short time. It can be carried out with
A major effect is that it does not adversely affect the culture state of cells.

[Brief explanation of the drawing]

第１図、第２図はそれぞれ本考案のデイスペン
サーの正面図、斜視図、第３図は本考案の実施例
の説明図である。１……容器、２……円錐形部品、３……支持部
材、４……吐出チツプ、５……チユーブ、６……
ポンプ、７……リング状部材、１１……ウエル。 1 and 2 are respectively a front view and a perspective view of the dispenser of the present invention, and FIG. 3 is an explanatory diagram of an embodiment of the present invention. DESCRIPTION OF SYMBOLS 1... Container, 2... Conical part, 3... Support member, 4... Discharge tip, 5... Tube, 6...
Pump, 7...ring-shaped member, 11...well.

Claims

[Scope of utility model registration request]

In a dispenser having a culture solution dispensing tip at the tip of a tube, a conical part is disposed at the distal end of a support member extending downward from the dispensing tip, such that the distal end of the conical part faces the distal end of the dispensing tip. A cell culture fluid discharge dispenser characterized by being supported.