JPH044994Y2 - - Google Patents
Info
- Publication number
- JPH044994Y2 JPH044994Y2 JP224286U JP224286U JPH044994Y2 JP H044994 Y2 JPH044994 Y2 JP H044994Y2 JP 224286 U JP224286 U JP 224286U JP 224286 U JP224286 U JP 224286U JP H044994 Y2 JPH044994 Y2 JP H044994Y2
- Authority
- JP
- Japan
- Prior art keywords
- cylinder
- centrifuge tube
- tube
- holder
- screw
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 239000007788 liquid Substances 0.000 claims description 13
- 238000000926 separation method Methods 0.000 claims description 8
- 238000005119 centrifugation Methods 0.000 claims description 3
- 238000000432 density-gradient centrifugation Methods 0.000 claims description 2
- 238000012805 post-processing Methods 0.000 claims 1
- 238000005194 fractionation Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 1
- 239000012780 transparent material Substances 0.000 description 1
Landscapes
- Sampling And Sample Adjustment (AREA)
- Centrifugal Separators (AREA)
Description
【考案の詳細な説明】
〔考案の利用分野〕
本考案は密度勾配遠心分離後の遠心管より分離
層を分取する装置に関するものである。[Detailed Description of the Invention] [Field of Application of the Invention] The present invention relates to an apparatus for separating a separated layer from a centrifuge tube after density gradient centrifugation.
従来の分取法は遠心分離後、遠心管の底部に穴
を明けて滴下する液を分取するか、遠心管に細管
を挿入しポンプなどにより吸取る方法などが主で
あつた。前者は遠心管に穴を明けてしまうため再
使用ができない欠点があつた。また後者の方法は
ポンプを用いるため駆動用の電源などを用いる不
便さがあつた。
Conventional preparative methods have mainly involved making a hole at the bottom of a centrifuge tube and collecting the dripping liquid after centrifugation, or inserting a thin tube into the centrifuge tube and sucking it up with a pump or the like. The former had the disadvantage that it could not be reused because it created a hole in the centrifuge tube. Furthermore, since the latter method uses a pump, it is inconvenient to require a power source for driving.
本考案は電源を用いず、手動式により分離層を
乱すことなく確実に分取できるようにしたことで
ある。
The present invention enables reliable separation without disturbing the separation layer using a manual method without using a power source.
本考案は遠心分離後の遠心管を透明のホルダー
で保持し、上部からシリンダーを挿入するように
し、シリンダーの先端部にＯリングを設け遠心管
内液がスキマより吐出しないようにし、シリンダ
先端部に細管を設け、細管を通つてシリンダー内
に液が留まるようにした。遠心管は底部に設けて
あるスクリユーを回転することにより上方に移動
する。遠心管内液はシリンダーの細管を通つて上
方に移動する。遠心管内液は細管部だけからしか
移動しないため分離層を乱すことはない。シリン
ダ内の液は細管を介して上方にあるため下層とは
混合しないように工夫した。
In this invention, the centrifuge tube after centrifugation is held in a transparent holder, a cylinder is inserted from the top, and an O-ring is installed at the tip of the cylinder to prevent the liquid in the centrifuge tube from being discharged from the gap. A capillary was provided so that the liquid remained in the cylinder through the capillary. The centrifuge tube is moved upward by rotating a screw provided at the bottom. The liquid in the centrifugal tube moves upward through the capillary of the cylinder. Since the liquid in the centrifugal tube moves only from the thin tube portion, it does not disturb the separation layer. Since the liquid inside the cylinder is located above through a thin tube, we have devised a way to prevent it from mixing with the lower layer.
本考案の実施例を図面により説明する。ベース
１には主栓２と主栓３、ネジ栓４が固定されてい
る。ネジ栓４には回転できるスクリユー５が取付
けてある。主栓２、栓３はベース６に固定されて
いる。ベース６には遠心管のサイズに合せ交換で
きるようにしたホルダー７が取付けてある。ホル
ダー７の底部の内側にはスクリユー５の上部に取
付けたチユーブホルダー９が設けてある。チユー
ブホルダー９はスリツプリング８を介して止めて
ある。スクリユー５は回転してもチユーブホルダ
ーは回転しないようにしてある。主栓２にはアツ
パーホルダー１０が止ネジ１２で固定されてい
る。アツパーホルダ１０にはシリンダー１５が固
定できるようにしてある。シリンダー１５にはO〓
リング１７と細管１６が設けてあり、遠心管１３
の上部に入るようにしてある。分離後の遠心管１
３をホルダー７に挿入し、シリンダー１５を上部
より遠心管１３の中に入れ、アツパープレート１
０で固定する。スクリユー５を回転することによ
り分離液１４はシリンダー１５の細管１６を通つ
てシリンダー内に分取液１８として留る。分取液
１８はアツパーホルダー１０の穴１１よりピペツ
トなどにより分取される。スクリユー５を順々に
回転することにより、断続的に分取ができる。
Embodiments of the present invention will be described with reference to the drawings. A main stopper 2, a main stopper 3, and a screw stopper 4 are fixed to the base 1. A rotatable screw 5 is attached to the screw plug 4. The main stopper 2 and stopper 3 are fixed to a base 6. A holder 7 is attached to the base 6 and is replaceable according to the size of the centrifuge tube. A tube holder 9 attached to the top of the screw 5 is provided inside the bottom of the holder 7. The tube holder 9 is fixed via a slip ring 8. Even if the screw 5 rotates, the tube holder does not rotate. An upper holder 10 is fixed to the main stopper 2 with a set screw 12. A cylinder 15 can be fixed to the upper holder 10. O for cylinder 15
A ring 17 and a thin tube 16 are provided, and a centrifugal tube 13
It is designed to fit at the top of the Centrifuge tube 1 after separation
3 into the holder 7, put the cylinder 15 into the centrifuge tube 13 from the top, and insert the upper plate 1 into the centrifuge tube 13.
Fixed at 0. By rotating the screw 5, the separated liquid 14 passes through the capillary tube 16 of the cylinder 15 and remains in the cylinder as a fractionated liquid 18. The fractionated liquid 18 is fractionated from the hole 11 of the upper holder 10 using a pipette or the like. By sequentially rotating the screw 5, it is possible to perform fractionation intermittently.
本考案は遠心管を上方に移動するようにしたも
のであるが、シリンダーを下方に移動するように
しても同一効果をもつ。 In the present invention, the centrifuge tube is moved upward, but the same effect can be obtained by moving the cylinder downward.
また、遠心管を移動する際、移動距離を知るた
めホルダーを透明な材料で製作し、スケールなど
を設けて分取精度を向上させることもできる。 Furthermore, when moving the centrifuge tube, the holder can be made of a transparent material and a scale etc. can be installed to improve separation accuracy in order to know the distance traveled.
本考案によれば電動式のポンプなどを用いるこ
となく分取することができ、どんな場所でも操作
できる特長がある。また、機構が簡単なため安値
に製作できる。
According to the present invention, it is possible to perform fractionation without using an electric pump, etc., and it has the advantage that it can be operated at any location. Moreover, since the mechanism is simple, it can be manufactured at low cost.
第１図は本考案による実施例を示す断面図であ
る。
図において、１はベース、２は主栓、３は栓、
４はネジ栓、５はスクリユー、６はベース、７は
ホルダー、８はスリツプリング、９はチユーブホ
ルダー、１０はアツパーホルダー、１１は穴、１
２は止ネジ、１３は遠心管、１４は分離液、１５
はシリンダー、１６は細管、１７はＯリング、１
８は分取液である。
FIG. 1 is a sectional view showing an embodiment of the present invention. In the figure, 1 is the base, 2 is the main stopper, 3 is the stopper,
4 is a screw plug, 5 is a screw, 6 is a base, 7 is a holder, 8 is a slip ring, 9 is a tube holder, 10 is an upper holder, 11 is a hole, 1
2 is a set screw, 13 is a centrifuge tube, 14 is a separation liquid, 15
is a cylinder, 16 is a thin tube, 17 is an O-ring, 1
8 is a fractionated liquid.
Claims (1)
として遠心管の分離層を分取する装置において、
分離層を乱すことなく遠心管上部にシリンダーを
挿入し、遠心管底部のスクリユーを回転すること
により、分離液をシリンダーに留め、順々にシリ
ンダー内より注射器などにより分取できるように
した遠心分離液分取装置。 In a device that separates the separated layer of a centrifuge tube as a post-processing after density gradient centrifugation using a centrifuge,
Centrifugation in which a cylinder is inserted into the top of the centrifuge tube without disturbing the separation layer, and by rotating the screw at the bottom of the centrifuge tube, the separated liquid is held in the cylinder and can be fractionated one by one from inside the cylinder using a syringe, etc. Liquid separation device.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP224286U JPH044994Y2 (en) | 1986-01-10 | 1986-01-10 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP224286U JPH044994Y2 (en) | 1986-01-10 | 1986-01-10 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPS62114341U JPS62114341U (en) | 1987-07-21 |
| JPH044994Y2 true JPH044994Y2 (en) | 1992-02-13 |
Family
ID=30780898
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP224286U Expired JPH044994Y2 (en) | 1986-01-10 | 1986-01-10 |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH044994Y2 (en) |
-
1986
- 1986-01-10 JP JP224286U patent/JPH044994Y2/ja not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| JPS62114341U (en) | 1987-07-21 |
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