JPH04503118A - 蛍光偏光の測定のための光学機器および方法 - Google Patents
蛍光偏光の測定のための光学機器および方法Info
- Publication number
- JPH04503118A JPH04503118A JP50109290A JP50109290A JPH04503118A JP H04503118 A JPH04503118 A JP H04503118A JP 50109290 A JP50109290 A JP 50109290A JP 50109290 A JP50109290 A JP 50109290A JP H04503118 A JPH04503118 A JP H04503118A
- Authority
- JP
- Japan
- Prior art keywords
- light
- source
- polarization
- polarized
- fluorophore
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000005259 measurement Methods 0.000 title claims description 27
- 238000002875 fluorescence polarization Methods 0.000 title claims description 15
- 238000000034 method Methods 0.000 title claims description 14
- 230000003287 optical effect Effects 0.000 title description 10
- 230000010287 polarization Effects 0.000 claims description 51
- 230000004907 flux Effects 0.000 claims description 23
- 230000005855 radiation Effects 0.000 claims description 2
- 238000012544 monitoring process Methods 0.000 claims 1
- 238000002834 transmittance Methods 0.000 description 6
- 238000001514 detection method Methods 0.000 description 5
- 230000005540 biological transmission Effects 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 230000005284 excitation Effects 0.000 description 3
- 230000005281 excited state Effects 0.000 description 3
- 230000007423 decrease Effects 0.000 description 2
- 238000009792 diffusion process Methods 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 229920001296 polysiloxane Polymers 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 210000002784 stomach Anatomy 0.000 description 2
- 241000282345 Meles Species 0.000 description 1
- 230000003750 conditioning effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 229910052736 halogen Inorganic materials 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 229910052721 tungsten Inorganic materials 0.000 description 1
- 239000010937 tungsten Substances 0.000 description 1
- -1 tungsten halogen Chemical class 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6445—Measuring fluorescence polarisation
Landscapes
- Health & Medical Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Physics & Mathematics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US43979589A | 1989-11-21 | 1989-11-21 | |
| US439,795 | 1989-11-21 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| JPH04503118A true JPH04503118A (ja) | 1992-06-04 |
Family
ID=23746168
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP50109290A Pending JPH04503118A (ja) | 1989-11-21 | 1990-11-21 | 蛍光偏光の測定のための光学機器および方法 |
Country Status (3)
| Country | Link |
|---|---|
| EP (1) | EP0454834A1 (fr) |
| JP (1) | JPH04503118A (fr) |
| WO (1) | WO1991007652A1 (fr) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2007505307A (ja) * | 2003-09-10 | 2007-03-08 | サーモ エレクトロン オイ | 偏光蛍光計 |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS55109949A (en) * | 1979-02-16 | 1980-08-23 | Hitachi Ltd | Spectro-fluorophotometer |
| CA1165585A (fr) * | 1981-01-09 | 1984-04-17 | Susan R. Popelka | Appareil optique pour instrument de polarisation par fluorescence |
-
1990
- 1990-11-21 JP JP50109290A patent/JPH04503118A/ja active Pending
- 1990-11-21 WO PCT/US1990/006864 patent/WO1991007652A1/fr not_active Ceased
- 1990-11-21 EP EP19910900498 patent/EP0454834A1/fr not_active Withdrawn
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2007505307A (ja) * | 2003-09-10 | 2007-03-08 | サーモ エレクトロン オイ | 偏光蛍光計 |
Also Published As
| Publication number | Publication date |
|---|---|
| WO1991007652A1 (fr) | 1991-05-30 |
| EP0454834A1 (fr) | 1991-11-06 |
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