JPH0459450U - - Google Patents

Info

Publication number
JPH0459450U
JPH0459450U JP1990102710U JP10271090U JPH0459450U JP H0459450 U JPH0459450 U JP H0459450U JP 1990102710 U JP1990102710 U JP 1990102710U JP 10271090 U JP10271090 U JP 10271090U JP H0459450 U JPH0459450 U JP H0459450U
Authority
JP
Japan
Prior art keywords
liquid
measurement passage
sample liquid
particle analyzer
introduction passages
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP1990102710U
Other languages
Japanese (ja)
Other versions
JPH0738838Y2 (en
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed filed Critical
Priority to JP1990102710U priority Critical patent/JPH0738838Y2/en
Priority to US07/733,612 priority patent/US5173740A/en
Priority to CA002049498A priority patent/CA2049498A1/en
Priority to DE69115415T priority patent/DE69115415T2/en
Priority to EP91308932A priority patent/EP0478392B1/en
Publication of JPH0459450U publication Critical patent/JPH0459450U/ja
Application granted granted Critical
Publication of JPH0738838Y2 publication Critical patent/JPH0738838Y2/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1404—Handling flow, e.g. hydrodynamic focusing

Landscapes

  • Chemical & Material Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Optical Measuring Cells (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Description

【図面の簡単な説明】[Brief explanation of drawings]

第１図は本考案の粒子分析装置用フローセルの
一実施例を示す断面説明図、第２図は第１図にお
ける測定用通路部分の斜視図、第３図及び第４図
は第１図におけるフローセルの液の流れを説明す
るための図、第５図及び第６図は別の流れの説明
をするための図である（第３図、第５図は第１の
状態を示し、第４図、第６図は第２の状態を示し
ている）。第７図は本考案の粒子分析装置用フロ
ーセルの他の実施例を示す説明図、第８図は第１
図〜第６図に示すフローセルを用いた粒子分析装
置の流体回路図、第９図は第７図に示すフローセ
ルを用いた粒子分析装置の流体回路図である。 １０……フローセル、１２……測定用通路、１
４，１６……導入用通路、１８，２０……試料液
用ノズル、２２，２６……シース液流入口、２４
，２８……廃液流出口、２９……発光素子、３１
……受光素子、３０，３２，３４，３６……試料
液、４０，４２……外筒、４４……定電流源、４
６……検出手段、Ｖ１〜Ｖ１４……弁、Ｓ１，Ｓ
２……シース液チヤンバ、Ｓ３，Ｓ４……試料液
チヤンバ、Ｓ５……シース液タンク、Ｗ１，Ｗ２
……廃液チヤンバ、Ｃ１，Ｃ２……シリンジ。 FIG. 1 is a cross-sectional explanatory diagram showing one embodiment of the flow cell for a particle analyzer of the present invention, FIG. 2 is a perspective view of the measurement passage in FIG. 1, and FIGS. 3 and 4 are the same as in FIG. Figures 5 and 6 are diagrams for explaining the flow of liquid in the flow cell, and Figures 5 and 6 are diagrams for explaining other flows (Figures 3 and 5 show the first state; 6 shows the second state). FIG. 7 is an explanatory diagram showing another embodiment of the flow cell for a particle analyzer according to the present invention, and FIG.
9 is a fluid circuit diagram of a particle analyzer using the flow cell shown in FIG. 7. FIG. 9 is a fluid circuit diagram of a particle analyzer using the flow cell shown in FIG. 10...Flow cell, 12...Measurement passage, 1
4, 16...Introduction passage, 18, 20...Sample liquid nozzle, 22, 26...Sheath liquid inlet, 24
, 28... Waste liquid outlet, 29... Light emitting element, 31
... Light receiving element, 30, 32, 34, 36 ... Sample liquid, 40, 42 ... Outer cylinder, 44 ... Constant current source, 4
6...Detection means, V1 to V14...Valve, S1, S
2... Sheath liquid chamber, S3, S4... Sample liquid chamber, S5... Sheath liquid tank, W1, W2
...Waste liquid chamber, C1, C2...Syringe.

Claims (1)

【実用新案登録請求の範囲】 １ 粒子を含む試料液の周囲にシース液を流し、
細流にされた試料液に光を照射し、粒子からの光
を個々に検出する粒子分析装置において、 幅の狭い測定用通路１２と、 この測定用通路１２に連なり測定用通路１２に
液を導入するための導入用通路１４，１６と、 測定用通路１２を挟んで先端が対向するように
導入用通路１４，１６内にそれぞれ配置・保持さ
れた試料液用ノズル１８，２０と、 導入用通路１４，１６にそれぞれ設けられたシ
ース液流入口２２，２６及び廃液流出口２４，２
８とを包含したフローセル１０を備えたことを特
徴とする粒子分析装置。 ２ 幅の狭い測定用通路１２と、 この測定用通路１２に連なり測定用通路１２に
液を導入するための導入用通路１４，１６と、 測定用通路１２を挟んで先端が対向するように
導入用通路１４，１６内にそれぞれ配置・保持さ
れた試料液用ノズル１８，２０と、 導入用通路１４，１６にそれぞれ設けられたシ
ース液流入口２２，２６及び廃液流出口２４，２
８とを包含することを特徴とする粒子分析装置用
フローセル。 ３ 試料液用ノズル１８，２０をそれぞれ囲むよ
うに外筒４０，４２を設け、シース液流入口２２
，２６をそれぞれ外筒４０，４２の外側に、廃液
流出口２４，２８をそれぞれ外筒４０，４２の内
側に設けたことを特徴とする請求項２記載の粒子
分析装置用フローセル。 ４ 試料液用ノズル１８，２０を導電性材料で形
成したことを特徴とする請求項３記載の粒子分析
装置用フローセル。[Claims for Utility Model Registration] 1. Pour a sheath liquid around a sample liquid containing particles,
In a particle analyzer that irradiates a sample liquid in a trickle with light and detects light from each particle individually, a liquid is introduced into a narrow measurement passage 12 and a measurement passage 12 connected to this measurement passage 12. Sample liquid nozzles 18 and 20 are arranged and held in the introduction passages 14 and 16, respectively, with their tips facing each other across the measurement passage 12, and the introduction passage. Sheath liquid inlets 22, 26 and waste liquid outlets 24, 2 provided in 14, 16, respectively
8. A particle analyzer characterized by comprising a flow cell 10 including 8. 2. A narrow measurement passage 12, introduction passages 14 and 16 that are connected to this measurement passage 12 and for introducing liquid into the measurement passage 12, and introduction passages 14 and 16 with their tips facing each other with the measurement passage 12 in between. Sample liquid nozzles 18, 20 arranged and held in the introduction passages 14, 16, respectively, and sheath liquid inlets 22, 26 and waste liquid outlets 24, 2 provided in the introduction passages 14, 16, respectively.
8. A flow cell for a particle analyzer, comprising: 3 Outer cylinders 40 and 42 are provided to surround the sample liquid nozzles 18 and 20, respectively, and the sheath liquid inlet 22
, 26 are provided on the outside of the outer cylinders 40, 42, respectively, and the waste liquid outlet ports 24, 28 are provided on the inside of the outer cylinders 40, 42, respectively. 4. The flow cell for a particle analyzer according to claim 3, wherein the sample liquid nozzles 18 and 20 are made of a conductive material.
JP1990102710U 1990-09-28 1990-09-28 Particle analysis device and flow cell used in the device Expired - Fee Related JPH0738838Y2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
JP1990102710U JPH0738838Y2 (en) 1990-09-28 1990-09-28 Particle analysis device and flow cell used in the device
US07/733,612 US5173740A (en) 1990-09-28 1991-07-22 Liquid particle analyzer and flow cell therefor
CA002049498A CA2049498A1 (en) 1990-09-28 1991-08-19 Particle analyzer and flow cell therefor
DE69115415T DE69115415T2 (en) 1990-09-28 1991-09-30 Particle analyzer and flow cell therefor
EP91308932A EP0478392B1 (en) 1990-09-28 1991-09-30 Particle analyzer and flow cell therefor

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
JP1990102710U JPH0738838Y2 (en) 1990-09-28 1990-09-28 Particle analysis device and flow cell used in the device

Publications (2)

Publication Number Publication Date
JPH0459450U true JPH0459450U (en) 1992-05-21
JPH0738838Y2 JPH0738838Y2 (en) 1995-09-06

Family

ID=14334830

Family Applications (1)

Application Number Title Priority Date Filing Date
JP1990102710U Expired - Fee Related JPH0738838Y2 (en) 1990-09-28 1990-09-28 Particle analysis device and flow cell used in the device

Country Status (5)

Country Link
US (1) US5173740A (en)
EP (1) EP0478392B1 (en)
JP (1) JPH0738838Y2 (en)
CA (1) CA2049498A1 (en)
DE (1) DE69115415T2 (en)

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2000214070A (en) * 1999-01-21 2000-08-04 Sysmex Corp See-through cell and blood analyzer using the same
JP2014081338A (en) * 2012-10-18 2014-05-08 Azbil Corp Method and device for discriminating air-borne/liquid-borne particles
JP2014149240A (en) * 2013-02-01 2014-08-21 Azbil Corp Particle detection system and particle detection method
JP2014153258A (en) * 2013-02-12 2014-08-25 Azbil Corp Particle detection system and particle detection method
WO2020039540A1 (en) * 2018-08-23 2020-02-27 アライドフロー株式会社 Flow cell, flow chamber, particle sorting device, and cartridge for particle sorting device
CN111526943A (en) * 2017-12-22 2020-08-11 Imec 非营利协会 Microfluidic routing
CN114632758A (en) * 2020-12-16 2022-06-17 深圳市帝迈生物技术有限公司 Cleaning device, liquid path cleaning method and sample analyzer

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5412466A (en) * 1991-07-26 1995-05-02 Toa Medical Electronics Co., Ltd. Apparatus for forming flattened sample flow for analyzing particles
US5395588A (en) * 1992-12-14 1995-03-07 Becton Dickinson And Company Control of flow cytometer having vacuum fluidics
JP3052665B2 (en) * 1993-01-26 2000-06-19 株式会社日立製作所 Flow cell device
JPH07120375A (en) * 1993-10-21 1995-05-12 Hitachi Ltd Flow type particle image analysis method and apparatus
US5895764A (en) * 1997-11-24 1999-04-20 University Of New Mexico Controlled sheath flow injection cytometry
ES2607527T3 (en) 2004-07-27 2017-03-31 Beckman Coulter, Inc. Discrimination improvement in flow cytometry with geometric transformation implemented by computer
US8062609B2 (en) 2006-11-10 2011-11-22 Luminex Corporation Flow cytometer and fluidic line assembly with multiple injection needles
JP6688089B2 (en) * 2016-01-22 2020-04-28 日本光電工業株式会社 Flow cell and particle analyzer
WO2019127563A1 (en) * 2017-12-30 2019-07-04 深圳迈瑞生物医疗电子股份有限公司 Particle analyzer using sheath flow impedance and measurement method therefor
CN113640198B (en) * 2021-07-26 2024-03-08 中国计量科学研究院 A method and system for single cell counting

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4515274A (en) * 1981-12-02 1985-05-07 Coulter Corporation Particle analyzing and sorting apparatus
US4790653A (en) * 1986-05-22 1988-12-13 Becton Dickinson And Company Housing for a flow cytometry apparatus with particle unclogging feature

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2000214070A (en) * 1999-01-21 2000-08-04 Sysmex Corp See-through cell and blood analyzer using the same
JP2014081338A (en) * 2012-10-18 2014-05-08 Azbil Corp Method and device for discriminating air-borne/liquid-borne particles
JP2014149240A (en) * 2013-02-01 2014-08-21 Azbil Corp Particle detection system and particle detection method
JP2014153258A (en) * 2013-02-12 2014-08-25 Azbil Corp Particle detection system and particle detection method
CN111526943A (en) * 2017-12-22 2020-08-11 Imec 非营利协会 Microfluidic routing
WO2020039540A1 (en) * 2018-08-23 2020-02-27 アライドフロー株式会社 Flow cell, flow chamber, particle sorting device, and cartridge for particle sorting device
JPWO2020039540A1 (en) * 2018-08-23 2021-08-10 アライドフロー株式会社 Flow cell, flow chamber, particle separator and cartridge for particle separator
US11169072B1 (en) 2018-08-23 2021-11-09 Allied Flow Inc. Flow cell, flow chamber, particle sorting apparatus, and particle sorting apparatus cartridge
CN114632758A (en) * 2020-12-16 2022-06-17 深圳市帝迈生物技术有限公司 Cleaning device, liquid path cleaning method and sample analyzer
CN114632758B (en) * 2020-12-16 2024-04-09 深圳市帝迈生物技术有限公司 Cleaning device, liquid path cleaning method and sample analyzer

Also Published As

Publication number Publication date
EP0478392A2 (en) 1992-04-01
EP0478392A3 (en) 1992-10-21
DE69115415T2 (en) 1996-05-02
CA2049498A1 (en) 1992-03-29
JPH0738838Y2 (en) 1995-09-06
US5173740A (en) 1992-12-22
EP0478392B1 (en) 1995-12-13
DE69115415D1 (en) 1996-01-25

Similar Documents

Publication Publication Date Title
US6919046B2 (en) Microfluidic analytical devices and methods
US5141719A (en) Multi-sample filtration plate assembly
US6582576B1 (en) Controller/detector interfaces for microfluidic systems
US5681438A (en) Membrane module assembly
JPH0580032A (en) Device for preparing sample, particularly for analytical purpose
US20020125139A1 (en) Methods and devices for high throughput fluid delivery
US4863696A (en) Apparatus for the percutaneous absorption of fluids
IL43405A (en) Enclosed filtering units for liquids or gases
WO1996012547B1 (en) Fluid processing apparatus
US5401637A (en) Device for conducting diagnostic assays
JPH0738838Y2 (en) Particle analysis device and flow cell used in the device
JPS59203653A (en) Particle sorting apparatus and method by gas generating action
DE3066583D1 (en) Apparatus for counting particles suspended in electrically conductive sample liquids
DE68920752D1 (en) VALVE FOR TRANSFER AND DOSING OF LIQUIDS.
JP2003066005A (en) Cell electrophoresis method and apparatus
CN115975782A (en) A reagent cartridge for genetic testing
JPS54116997A (en) Analyzer
JPH11183359A5 (en)
JPS6140541A (en) Measuring device for predetermined characteristic of suspended particle in carrying medium
JPH10318906A (en) Sheath flow type particle analyzer
JPH062680U (en) Semiconductor manufacturing equipment
JPH0539663Y2 (en)
JP2520493Y2 (en) Switching valve
WO2002058845A3 (en) Reaction vessel and system incorporating same
CN220990749U (en) Microchannel reactor

Legal Events

Date Code Title Description
LAPS Cancellation because of no payment of annual fees