JPH0465675A - Hemolysis tester - Google Patents
Hemolysis testerInfo
- Publication number
- JPH0465675A JPH0465675A JP17739290A JP17739290A JPH0465675A JP H0465675 A JPH0465675 A JP H0465675A JP 17739290 A JP17739290 A JP 17739290A JP 17739290 A JP17739290 A JP 17739290A JP H0465675 A JPH0465675 A JP H0465675A
- Authority
- JP
- Japan
- Prior art keywords
- container
- liquid
- elastic membrane
- blood
- sealed container
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
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- Investigating Or Analysing Biological Materials (AREA)
Abstract
(57)【要約】本公報は電子出願前の出願データであるた
め要約のデータは記録されません。(57) [Summary] This bulletin contains application data before electronic filing, so abstract data is not recorded.
Description
【発明の詳細な説明】
〔産業上の利用分野〕
本発明は、心臓手術などで体外循環又は補助循環装置を
必要とする場合において、手術前になるべく簡便な方法
で患者血液を検査し、該血液の機械的損傷に対する赤血
球の被溶血性をテストし、その結果を基に補助循環ポン
プの操作法を決定するなどの用途に用いる。なお、上記
「溶血」とは、血色素の放出を伴う赤血球の溶解又は破
壊をいう。[Detailed Description of the Invention] [Industrial Application Field] The present invention, in cases where extracorporeal circulation or auxiliary circulation equipment is required in cardiac surgery, etc., tests the patient's blood in the simplest possible way before the surgery, and This test tests the susceptibility of red blood cells to hemolysis due to mechanical damage, and the results are used for purposes such as deciding how to operate an auxiliary circulation pump. Note that the above-mentioned "hemolysis" refers to the lysis or destruction of red blood cells accompanied by the release of hemoglobin.
〔従来の技術及び発明が解決しようとする課題〕従来、
血球の破壊に関する試験は、殆んど化学的環境変化によ
るものであり、化学的要因に基づく血球の破壊を調べる
装置しかなく、本発明装置のような方法で機械的侵襲（
ｉｎｖａｓｉｏｎ、侵入）による赤血球の被溶血性を試
験できる装置、つまり血球の機械的破壊を調べる装置は
なかった。[Problems to be solved by conventional techniques and inventions] Conventionally,
Most tests for the destruction of blood cells are based on changes in the chemical environment, and there are only devices that examine the destruction of blood cells based on chemical factors.
There was no device capable of testing the susceptibility of red blood cells to hemolysis due to invasion (invasion), that is, a device for examining mechanical destruction of blood cells.
本発明は、ベツドサイドにおいてなるべく簡便な装置に
よって、溶血性を判断する溶血試験装置を提供すること
を目的としており、試験環境温度、平均圧力及び圧力振
幅の最大値・最小値、振動数などを任意に設定できると
共に、同時に複数の検体について試験できる溶血試験装
置を提供することを技術的課題としている。The purpose of the present invention is to provide a hemolysis test device for determining hemolysis using a device as simple as possible at the bedside. Our technical objective is to provide a hemolysis test device that can be set to 100% and simultaneously test multiple specimens.
上記の技術的課題を解決するために、本発明は、密閉容
器の一部、通常底部を弾性膜によって構成し、該弾性膜
を加振棒を介して外部から加振できるようにし、該密閉
容器内に、弾性チューブ等の弾性体で作った密閉可能な
血液検体容器を入れ、且つ該密閉容器“内に水などの液
体を満たす構造にして、試験環境温度、平均圧力、圧力
振幅の最大値・最小値及び振動数などを任意に設定でき
るようにしたことを特徴としている。 、また、液
体を満たすようにした上記密閉容器内に熱交換器を設置
して、該密閉容器内の液温を調節できるようにしたこと
を特徴としている。In order to solve the above-mentioned technical problem, the present invention comprises a part of the sealed container, usually the bottom part, of an elastic membrane, and makes it possible to vibrate the elastic membrane from the outside via a vibrating rod, so that the sealed container is sealed. A sealable blood sample container made of an elastic material such as an elastic tube is placed inside the container, and the container is structured to be filled with a liquid such as water to maintain the test environment temperature, average pressure, and maximum pressure amplitude. It is characterized in that the value, minimum value, vibration frequency, etc. can be set arbitrarily.In addition, a heat exchanger is installed in the above-mentioned sealed container filled with liquid, and the liquid in the sealed container is The feature is that the temperature can be adjusted.
本発明は上記のように構成されているので、弾性膜を加
振棒を介して動電形加振器などの加振手段を用いて外部
から正弦波加振すると、密閉容器内の液体に、加振力に
応じた振動圧力が発生する。Since the present invention is configured as described above, when the elastic membrane is externally vibrated in a sine wave using a vibrating means such as an electrodynamic vibrator via a vibrating rod, the liquid in the closed container is , vibration pressure is generated according to the excitation force.
該密閉容器内に、血液を満たした弾−性体の検体容器を
設置すると、上記加振により発生した該密閉容器内の液
圧が検体容器に加わり、該検体容器内の血液にも、液圧
に応じた圧力変動が生じる。When an elastic specimen container filled with blood is placed in the sealed container, the fluid pressure in the sealed container generated by the above vibration is applied to the specimen container, causing the blood in the specimen container to be Pressure fluctuations occur depending on the pressure.
上記検査血液に加わる圧力の振幅及び振動数は、加振器
の入力を制御することによって容易に変えることができ
、また、圧力の最大値と最小値は、圧力振幅の平均値（
静水圧）を次のようにして変化させることにより、容易
に設定可能である。即ち、該密閉容器は壁面の一部が弾
性膜でてきているので、該密閉容器内に密閉される液体
の量を加減することにより、弾性膜の張力で静水圧を容
易に変化させることが可能である。The amplitude and frequency of the pressure applied to the test blood can be easily changed by controlling the input of the vibrator, and the maximum and minimum values of the pressure can be set to the average value of the pressure amplitude (
It can be easily set by changing the hydrostatic pressure in the following manner. That is, since a part of the wall surface of the sealed container has an elastic membrane, it is possible to easily change the hydrostatic pressure by adjusting the amount of liquid sealed in the sealed container using the tension of the elastic membrane. It is possible.
更に、検体容器は液体中に設置されてい・るので、該密
閉容器内の水温を熱交換器等により制御することによっ
て、試験環境温度を一定に保つ恒温槽の役目も兼ね備え
ることができる。また、密閉容器内に同じ検体容器を複
数個設置すれば、同一条件での同時試験が可能である。Furthermore, since the sample container is placed in the liquid, by controlling the water temperature in the sealed container with a heat exchanger or the like, it can also serve as a constant temperature bath to keep the test environment temperature constant. Furthermore, if multiple identical sample containers are installed in a sealed container, simultaneous testing under the same conditions is possible.
次に、本発明の実施例を、図面と共に説明する。 Next, embodiments of the present invention will be described with reference to the drawings.
第１図は、本発明の一実施例を示す縦断面図である。FIG. 1 is a longitudinal sectional view showing one embodiment of the present invention.
図において、ｌは密閉容器で、該密閉容器ｌの下端には
弾性膜２が取付けられており、該弾性膜２は、加振棒３
を介して、下方より動電形加振器４によって加振できる
ようになっている。In the figure, l is a closed container, and an elastic membrane 2 is attached to the lower end of the closed container l.
Vibration can be applied from below by an electrodynamic vibrator 4 via the .
上記密閉容器ｌの中には、弾性チューブで作られた検体
容器５が設置できるようになっており、該検体容器５は
、上部の活栓’６ ’＋−開いて血液を流入した□す、
該血液の圧力をモニターすることができるようになって
いる。A sample container 5 made of an elastic tube can be installed in the airtight container 1, and the sample container 5 is opened to allow blood to flow in by opening the stopcock '6' at the top of the sample container 5.
The pressure of the blood can be monitored.
また、上記密閉容器ｌの頂部及び下部側方部には、注入
用バルブ７及び排出用バルブ８がそれぞれ設けられてお
り、これらのバルブ７及び８の開閉により、該密閉容器
ｌ内の水量を調節して、容器１内部の静水圧を変化させ
ることができるようになっている。また、上記弾性チュ
ーブで作られｄ振”動□数は、当該装置の外部に設置さ
れ且つ加振器４に接続されたコントローラ９によって、
調節される。なお、図中、１０は、熱交換器で、該密閉
容器ｌ内の液体温度を調節するものである。Further, an injection valve 7 and a discharge valve 8 are provided at the top and lower side parts of the sealed container l, respectively, and by opening and closing these valves 7 and 8, the amount of water in the sealed container l is controlled. It is possible to adjust the hydrostatic pressure inside the container 1 to change it. In addition, the d vibration frequency made by the elastic tube is controlled by a controller 9 installed outside the device and connected to the vibrator 4.
adjusted. In addition, in the figure, 10 is a heat exchanger that adjusts the temperature of the liquid in the closed container l.
上ｉ己のような構成により、血□ｉを満たした検体容器
５′を内部゛に設置した密閉容器ｌ内に″、液体として
水を充満し、弾性膜２を動電形加振器４によって正弦波
加振すると、容器１内の液体に加振力に応した振動圧力
が発生し、弾性体からなる血液検査容器５内の血液にも
、上記液圧に応じた圧力変動を生じる。With the configuration shown above, water is filled as a liquid in the sealed container l in which the sample container 5' filled with blood i is placed inside, and the elastic membrane 2 is connected to the electrodynamic vibrator 4. When a sinusoidal wave is excited, a vibration pressure corresponding to the excitation force is generated in the liquid in the container 1, and pressure fluctuations corresponding to the liquid pressure are also generated in the blood in the blood test container 5 made of an elastic body.
この際、該検査血液に加わる圧力の振幅及び振動数は、
加振器・４の入力をコントローラ９により制御すること
により、容易に変化でき、また、圧力の最大値と最小値
は、該密閉容器ｌ内の水量をバルブ７．８を藺閉して加
減することにより弾性膜２の張力で静水圧を変化させて
圧力振幅の平均値を変化させることにより、容易に設定
することができる。At this time, the amplitude and frequency of the pressure applied to the test blood are:
The input to the vibrator 4 can be easily changed by controlling the controller 9, and the maximum and minimum pressure values can be adjusted by adjusting the amount of water in the sealed container by closing the valve 7.8. This allows easy setting by changing the hydrostatic pressure using the tension of the elastic membrane 2 and changing the average value of the pressure amplitude.
更に、検体容器５は水中に設置されているので、該密閉
容器１内の水温を熱交換器１０によ′つて制御すること
により、試験環境温度を一定に保つ恒温槽の゛役目をも
゛兼ね備えている。Furthermore, since the sample container 5 is placed underwater, by controlling the water temperature in the sealed container 1 using the heat exchanger 10, it also functions as a constant temperature bath to keep the test environment temperature constant. It has both.
上記した実施例において、密閉容器ｌ内に充満する液体
として水を用いた例について説明したが、水辺外の例え
ば、グリセリン溶液やシリコン油のような液体でもよい
。In the above-mentioned embodiment, an example was explained in which water was used as the liquid filling the closed container 1, but it may also be a liquid outside the water, such as a glycerin solution or silicone oil.
また、弾性膜２は、密閉容器ｌの底部以外の側壁の一部
に設けてもよい。Further, the elastic membrane 2 may be provided on a part of the side wall of the closed container l other than the bottom part.
また、検体容器５を密閉容器１内に１個設置した構造に
ついて説明したが、複数個設置することが可能で、この
場合、同一条件での同時試験が可能である。Further, although the structure in which one specimen container 5 is installed in the closed container 1 has been described, it is possible to install a plurality of specimen containers 5, and in this case, simultaneous testing under the same conditions is possible.
以上説明したように、本発明によれば、弾性体からなる
密閉可能な血液検体容器を、壁面の一部を弾性膜によっ
て構成し内部に液体を満たすようにした密閉容器内に設
置し、上記弾性膜を外部から加振できるようにした、こ
とにより、上記密閉容器内の液体を介して、血液検体容
器内の血液に任意の振動数と振幅の圧力変動を加えるこ
とができ、これにより、簡単な装置によって、機械的侵
襲による赤血球の被溶血性を試験することができる。As explained above, according to the present invention, a sealable blood sample container made of an elastic body is installed in a sealed container whose wall surface is partially composed of an elastic membrane and the inside is filled with liquid. The elastic membrane can be vibrated from the outside, thereby making it possible to apply pressure fluctuations of arbitrary frequency and amplitude to the blood in the blood sample container via the liquid in the sealed container. A simple device allows testing the susceptibility of red blood cells to hemolysis by mechanical attack.
従って、ベツドサイドにおいて簡便な当該溶血試験装置
によって、溶血性を判断することができるばかりでなく
、同時に複数の検体について試験することができる。Therefore, by using the hemolysis test device, which is simple and convenient at the bedside, not only hemolysis can be determined, but also a plurality of specimens can be tested at the same time.
また、密閉容器内に熱交換器を設置することにより、該
密閉容器内の液温を制御して、試験環境温度を一定に保
つ恒温槽の役目をも兼備させることができる。Furthermore, by installing a heat exchanger inside the closed container, the temperature of the liquid inside the closed container can be controlled and the test environment temperature can be kept constant so that the heat exchanger can also function as a constant temperature bath.
第１図は本発明の一実施例を示す溶血試験装置の縦断面
図である。
ｌ−・・密閉容器、２・・・弾性膜、３・・・加振棒、
４・・・加振器、５・・・検体容器、７，８・・・バル
ブ、９・・・コントローラ、ｌＯ・・・熱交換器。
二６４４−FIG. 1 is a longitudinal sectional view of a hemolysis test device showing an embodiment of the present invention. l-... airtight container, 2... elastic membrane, 3... vibrating rod,
4... Exciter, 5... Sample container, 7, 8... Valve, 9... Controller, lO... Heat exchanger. 2644-
Claims (1)
一部を弾性膜によって構成し内部に液体を満たすように
した密閉容器内に設置し、上記弾性膜を外部から加振で
きるようにし、該密閉容器内に液体を充満し上記弾性膜
を外部から加振することにより、該密閉容器内の液体を
介して血液検体容器内の血液に任意の振動数と振幅の圧
力変動を加えるようにしたことを特徴とする溶血試験装
置。 ２、密閉容器内に熱交換器を設けたことを特徴とする請
求項１記載の溶血試験装置。[Scope of Claims] 1. A sealable blood sample container made of an elastic material is installed in a sealed container whose wall is partially made up of an elastic membrane and the inside is filled with liquid, and the elastic membrane is placed outside the container. By filling the sealed container with liquid and exciting the elastic membrane from the outside, the blood in the blood sample container can be vibrated at any frequency and amplitude through the liquid in the sealed container. A hemolysis test device characterized in that it applies pressure fluctuations of . 2. The hemolysis test device according to claim 1, further comprising a heat exchanger provided within the closed container.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP17739290A JPH0652267B2 (en) | 1990-07-06 | 1990-07-06 | Hemolysis test equipment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP17739290A JPH0652267B2 (en) | 1990-07-06 | 1990-07-06 | Hemolysis test equipment |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH0465675A true JPH0465675A (en) | 1992-03-02 |
| JPH0652267B2 JPH0652267B2 (en) | 1994-07-06 |
Family
ID=16030137
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP17739290A Expired - Fee Related JPH0652267B2 (en) | 1990-07-06 | 1990-07-06 | Hemolysis test equipment |
Country Status (1)
| Country | Link |
|---|---|
| JP (1) | JPH0652267B2 (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2758497A1 (en) * | 1976-12-31 | 1978-07-13 | Alkor Gmbh | THERMOPLASTIC MOLDING COMPOUNDS, THEIR USE AND FILMS FROM THE MOLDING COMPOUND |
| JP2010117355A (en) * | 2008-11-12 | 2010-05-27 | F Hoffmann La Roche Ag | Hemolyzer |
-
1990
- 1990-07-06 JP JP17739290A patent/JPH0652267B2/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2758497A1 (en) * | 1976-12-31 | 1978-07-13 | Alkor Gmbh | THERMOPLASTIC MOLDING COMPOUNDS, THEIR USE AND FILMS FROM THE MOLDING COMPOUND |
| JP2010117355A (en) * | 2008-11-12 | 2010-05-27 | F Hoffmann La Roche Ag | Hemolyzer |
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0652267B2 (en) | 1994-07-06 |
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