JPH0477722B2 - - Google Patents

Abstract

A process for combating Varoa Jacobsoni on bees, comprising applying to the bees or their habitat an amount effective therefor of 0,0-diethyl 0-(3-chloro-4-methyl-7-coumarinyl) thionophosphate.

Description

DETAILED DESCRIPTION OF THE INVENTION The present invention provides a novel method for controlling Varoa Jacobsoni, a mite that attaches to honeybees, and a method for controlling 0,0-diethyl 0-(3-chloro-4-
Varoatosis agents containing methyl-7-coumarinyl) thiophosphate (Coumaphos). Varroa disease is caused by a species of mite, Varoa Jacobsoni. In its adult state, this mite parasitizes worker bees, male bees, and queen bees, and before it becomes an adult, it parasitizes worker bees, male bee larvae, and pupae. In addition to the direct damage caused by the mites, this weakens the pups and makes them more susceptible to viral and bacterial diseases. This parasitism is new to the beekeeping world, and is already widespread in the animal kingdom. Varroa disease has a devastating impact on beekeeping by rapidly weakening bee populations and killing many of them. Furthermore, this allows beekeeping products such as honey,
Beeswax, royal jelly, and bee poison are directly affected. In addition, pollination of insect-pollinated crops is reduced, which has a negative impact on the indirect use of bees. This indirect use is a substitute for direct production by bees.
Reaching 10-15 times more. Previously known methods of controlling Varroa disease involve smoking the bee population or spraying active compounds, which inevitably have negative effects. Methods of heat treatment and mechanical removal of male wasp larvae by opening the lid of their nests are also known. These known methods have the disadvantage that they require a large amount of input to carry out and therefore cause havoc in the population. The present invention provides 10 to 50 mg of 0,0-diethyl 0-(3
-Chloro-4-methyl-7-coumarinyl) thiophosphate (Coumaphos) with the same active compound 100 ~
The present invention relates to a method for controlling Varroa disease, which comprises irrigating, dropping, or spraying the aqueous suspension or emulsion containing 1000 ppm. The treatment method of the present invention is carried out 2 to 3 times at intervals of 4 to 20 days, preferably 1 to 2 weeks, during periods when spawning and rearing are slow, such as during wintering or resting periods. For each treatment, 30-60 ml of a diluted coumaphos aqueous suspension or emulsion is applied dropwise to the bee population. 50ml is preferred. The application is
This can be done by irrigation, dripping or spraying. This treatment results in the use of 10-50 mg coumaphos per population, preferably 15-30 mg. The concentration of active compound in dilute aqueous suspensions or emulsions is between 100 and 1000 ppm. Preferably 200~
700 ppm, particularly preferably about 500 ppm. Active compound concentration 0.1-20%, preferably 10-20
%, particularly preferably 16%, to prepare ready-to-use application forms. These dosage forms are prepared in known manner, for example by mixing the active compound with fillers, ie liquid solvents, and if appropriate using surfactants, ie emulsifiers and/or dispersants. When using water as extender, it is also possible, for example, to use organic solvents as co-solvents. Suitable liquid solvents include, for example, aromatic compounds such as xylene, toluene or alkylnaphthalenes, chlorinated aromatic or chlorinated aliphatic hydrocarbons such as chlorobenzene, chloroethylene or methylene chloride, aliphatic hydrocarbons such as e.g. Cyclohexane or paraffins such as petroleum distillates, alcohols such as butanol or glycols and their ethers and esters, ketones such as acetone, methyl ethyl ketone, methyl isobutyl ketone or cyclohexanone, highly polar solvents such as dimethylformamide, and dimethyl sulfoxide and water. be. Suitable emulsifiers include, for example, nonionic or anionic emulsifiers, such as alkylaryl polyglycol ethers, alkyl sulfonates, alkyl sulfates, arylsulfonates, and protein hydrolysates; dispersants include, for example, lignin sulfites. There is waste liquid and methylcellulose. atomizing, sprayig,
Alternatively, by fumigation, it is not necessary to wet each bee and the entire nest with a solution of the active compound. Surprisingly, coumaphos has systemic effects on both adult and larval bees. That is, the compound is absorbed into the body of the bee and transferred to the tick that sucks its body fluid. This way it works, there is no need to wet each bee and bring it into contact with the mites, which are sucking body fluids. This systemic effect in bees was surprisingly absent when coumaphos was used to control ectoparasites in warm-blooded animals. The composition of the stock solution for dilution with water to give an application form solution is shown below. Example 1 Coumaphos 20g Emulsifier Toximui (a mixture of calcium alkylbenzenesulfonate, nonionic emulsifier and methanol, HLB = hydrophilic/lipophilic balance): 10 = 7g Emulsifier Toximui S (calcium alkylbenzenesulfonate, nonionic emulsifier) , mixture of methanol, HLB: 10) 5 g Solvesso 200 (mixture of alkylnaphthalenes from high-boiling petroleum fractions)
100ml or less Example 2 Coumaphos 16g Emulsifier 368 Alkylaryl polyglycol ether (molecular weight 1165) 9g Emulsifier NP10 Nonylphenyl polyglycol ether 9g Dimethylformamide 10g Solvesso 200 100ml or less Example 3 Coumaphos 5g Emulsifier Atlox (mixture of polyoxyethylene ether, polyoxyglyceride, alkylaryl sulfonate - readily water soluble) 2g Emulsifier Atlox 3409 (mixture of nonionic and anionic emulsifier - water soluble) 4g Solvent PC2 (high boiling aromatic petroleum fraction )
100ml or less Example 4 Coumaphos 1g Emulsifier 368 30g Dowanol DPM (dipropylene glycol methyl ether) 100ml or less Example 5 Coumaphos 2.5g Emulsifier 368 10g Emulsifier NP10 10g Solvesso 200 20g Dowanol DPM 100 ml or less carried out Example 6 Coumaphos 2g Emulsifier 368 9g Emulsifier NP10 9g Solvesso 200 16g Dowanol DPM 45g Water 100ml or less Example 7 Coumaphos 1g Emulsifier 368 10g Emulsifier NP10 10g Solvesso 200 10 g Dowanol DPM 100ml or less Example 8 coumaphos 1g Emulsifier Tween80 (sorbitan monooleate HLB: 4.3) 8g emulsifier Span (sorbitan monooleate
HLB: 15) 4g N-methylpyrrolidone 100ml or less Example 9 coumaphos 1g cremophor HS15 (polyoxyethylene 600 hydroxystearate)
20g Chlorobenzene 100ml or less Example 10 Coumaphos 1g Emulsifier W (alkylaryl polyglycol ether, molecular weight approximately 853) 90g Dimethylisosorbitol 100ml or less It is clear from the following experiment that the treatment method of the present invention can exhibit excellent effects. It is. EXAMPLE A population of honey bees of the specified number was treated with 50 ml of coumaphos aqueous emulsion dropwise.
5ml of emulsion was dropped into each hole in the nest. The emulsion concentration in this experiment was 30ppm, 60ppm or
It is 90ppm. Treatments were performed three times at 5-day intervals. After each treatment, the number of dead mites and bees was counted. Once the process was completed, the bee group was killed. I counted the number of bees and washed the mites that were still attached to them from the bees. This allowed us to obtain the total number of mites that were initially attached. Bee and mite mortality rates were then calculated after each treatment. The table below shows the experimental results compared with commercially available agents. The commercially available product has the trade name FolbexVA and was used as a fumigation agent in the experiment according to the instructions. The active agent included in the commercial formulation is isopropyl 4,4'-dibromobendiatate. 【table】

Claims (1)

[Claims] 1. 10 to 50 mg of 0,0-diethyl 0-(3-chloro-4-methyl-7-coumarinyl)thionophosphate (Coumaphos) per treatment and per bee population. A method for controlling the honey bee mite, Varroa jacobsoni, characterized in that it is dipped, dripped or sprayed in the form of an aqueous suspension or emulsion containing 100 to 1000 ppm of the active compound.