JPH0478139B2 - - Google Patents

Info

Publication number: JPH0478139B2
Authority: JP; Japan
Prior art keywords: alginate; acid; gel; agarose; group
Prior art date: 1984-03-12
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired

Application number

JP60501380A

Other languages

English (en)

Japanese (ja)

Other versions

JPS61500810A (ja

Inventor

Kyashii Jei Urufuerudaa

Uiriamu Ee Gaasuku

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

SmithKline Beecham Corp

Original Assignee

SmithKline Beecham Corp

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1984-03-12

Filing date

1985-03-12

Publication date

1992-12-10

1985-03-12 Application filed by SmithKline Beecham Corp filed Critical SmithKline Beecham Corp

1986-04-24 Publication of JPS61500810A publication Critical patent/JPS61500810A/ja

1992-12-10 Publication of JPH0478139B2 publication Critical patent/JPH0478139B2/ja

Status Granted legal-status Critical Current

Links

239000000499 gel Substances 0.000 claims description 72
238000001962 electrophoresis Methods 0.000 claims description 32
229920000936 Agarose Polymers 0.000 claims description 26
235000010443 alginic acid Nutrition 0.000 claims description 26
229920000615 alginic acid Polymers 0.000 claims description 26
229940072056 alginate Drugs 0.000 claims description 21
FHVDTGUDJYJELY-UHFFFAOYSA-N 6-{[2-carboxy-4,5-dihydroxy-6-(phosphanyloxy)oxan-3-yl]oxy}-4,5-dihydroxy-3-phosphanyloxane-2-carboxylic acid Chemical class O1C(C(O)=O)C(P)C(O)C(O)C1OC1C(C(O)=O)OC(OP)C(O)C1O FHVDTGUDJYJELY-UHFFFAOYSA-N 0.000 claims description 20
238000000034 method Methods 0.000 claims description 19
-1 alginate ester Chemical class 0.000 claims description 14
150000004676 glycans Chemical class 0.000 claims description 13
229920001282 polysaccharide Polymers 0.000 claims description 13
239000005017 polysaccharide Substances 0.000 claims description 13
150000001875 compounds Chemical class 0.000 claims description 12
DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 claims description 11
OFOBLEOULBTSOW-UHFFFAOYSA-N Propanedioic acid Natural products OC(=O)CC(O)=O OFOBLEOULBTSOW-UHFFFAOYSA-N 0.000 claims description 10
239000000872 buffer Substances 0.000 claims description 9
KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 9
IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims description 8
239000000661 sodium alginate Substances 0.000 claims description 8
235000010413 sodium alginate Nutrition 0.000 claims description 8
229940005550 sodium alginate Drugs 0.000 claims description 8
229920001817 Agar Polymers 0.000 claims description 7
239000008272 agar Substances 0.000 claims description 7
229920005862 polyol Polymers 0.000 claims description 7
239000003755 preservative agent Substances 0.000 claims description 7
WNLRTRBMVRJNCN-UHFFFAOYSA-N adipic acid Chemical compound OC(=O)CCCCC(O)=O WNLRTRBMVRJNCN-UHFFFAOYSA-N 0.000 claims description 6
239000000203 mixture Substances 0.000 claims description 6
KQTIIICEAUMSDG-UHFFFAOYSA-N tricarballylic acid Chemical compound OC(=O)CC(C(O)=O)CC(O)=O KQTIIICEAUMSDG-UHFFFAOYSA-N 0.000 claims description 6
KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims description 5
WHXSMMKQMYFTQS-UHFFFAOYSA-N Lithium Chemical group [Li] WHXSMMKQMYFTQS-UHFFFAOYSA-N 0.000 claims description 5
ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 5
150000003863 ammonium salts Chemical class 0.000 claims description 5
125000004432 carbon atom Chemical group C* 0.000 claims description 5
230000002335 preservative effect Effects 0.000 claims description 5
KDYFGRWQOYBRFD-UHFFFAOYSA-N succinic acid Chemical compound OC(=O)CCC(O)=O KDYFGRWQOYBRFD-UHFFFAOYSA-N 0.000 claims description 5
125000002887 hydroxy group Chemical group [H]O* 0.000 claims description 4
VZCYOOQTPOCHFL-UPHRSURJSA-N maleic acid Chemical compound OC(=O)\C=C/C(O)=O VZCYOOQTPOCHFL-UPHRSURJSA-N 0.000 claims description 4
239000011976 maleic acid Substances 0.000 claims description 4
159000000000 sodium salts Chemical class 0.000 claims description 4
VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 claims description 4
BJEPYKJPYRNKOW-REOHCLBHSA-N (S)-malic acid Chemical compound OC(=O)[C@@H](O)CC(O)=O BJEPYKJPYRNKOW-REOHCLBHSA-N 0.000 claims description 3
RTBFRGCFXZNCOE-UHFFFAOYSA-N 1-methylsulfonylpiperidin-4-one Chemical compound CS(=O)(=O)N1CCC(=O)CC1 RTBFRGCFXZNCOE-UHFFFAOYSA-N 0.000 claims description 3
JAHNSTQSQJOJLO-UHFFFAOYSA-N 2-(3-fluorophenyl)-1h-imidazole Chemical compound FC1=CC=CC(C=2NC=CN=2)=C1 JAHNSTQSQJOJLO-UHFFFAOYSA-N 0.000 claims description 3
239000001361 adipic acid Substances 0.000 claims description 3
235000011037 adipic acid Nutrition 0.000 claims description 3
BJEPYKJPYRNKOW-UHFFFAOYSA-N alpha-hydroxysuccinic acid Natural products OC(=O)C(O)CC(O)=O BJEPYKJPYRNKOW-UHFFFAOYSA-N 0.000 claims description 3
JFCQEDHGNNZCLN-UHFFFAOYSA-N anhydrous glutaric acid Natural products OC(=O)CCCC(O)=O JFCQEDHGNNZCLN-UHFFFAOYSA-N 0.000 claims description 3
239000001630 malic acid Substances 0.000 claims description 3
235000011090 malic acid Nutrition 0.000 claims description 3
LVHBHZANLOWSRM-UHFFFAOYSA-N methylenebutanedioic acid Natural products OC(=O)CC(=C)C(O)=O LVHBHZANLOWSRM-UHFFFAOYSA-N 0.000 claims description 3
JDVPQXZIJDEHAN-UHFFFAOYSA-N succinamic acid Chemical compound NC(=O)CCC(O)=O JDVPQXZIJDEHAN-UHFFFAOYSA-N 0.000 claims description 3
238000001035 drying Methods 0.000 claims description 2
230000005012 migration Effects 0.000 claims description 2
238000013508 migration Methods 0.000 claims description 2
229920001451 polypropylene glycol Polymers 0.000 claims description 2
DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims 4
229910052744 lithium Inorganic materials 0.000 claims 4
229910052700 potassium Inorganic materials 0.000 claims 4
239000011591 potassium Substances 0.000 claims 4
229910052708 sodium Inorganic materials 0.000 claims 4
239000011734 sodium Substances 0.000 claims 4
239000001384 succinic acid Substances 0.000 claims 2
239000007853 buffer solution Substances 0.000 claims 1
239000003792 electrolyte Substances 0.000 claims 1
102000001554 Hemoglobins Human genes 0.000 description 14
108010054147 Hemoglobins Proteins 0.000 description 14
KRKNYBCHXYNGOX-UHFFFAOYSA-K Citrate Chemical compound [O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O KRKNYBCHXYNGOX-UHFFFAOYSA-K 0.000 description 6
QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 5
230000015572 biosynthetic process Effects 0.000 description 4
LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 3
PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
210000004027 cell Anatomy 0.000 description 3
DNIAPMSPPWPWGF-GSVOUGTGSA-N (R)-(-)-Propylene glycol Chemical compound C[C@@H](O)CO DNIAPMSPPWPWGF-GSVOUGTGSA-N 0.000 description 2
LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 2
RTAQQCXQSZGOHL-UHFFFAOYSA-N Titanium Chemical compound [Ti] RTAQQCXQSZGOHL-UHFFFAOYSA-N 0.000 description 2
229960000583 acetic acid Drugs 0.000 description 2
239000000975 dye Substances 0.000 description 2
239000000834 fixative Substances 0.000 description 2
235000013772 propylene glycol Nutrition 0.000 description 2
239000000243 solution Substances 0.000 description 2
KEAYESYHFKHZAL-UHFFFAOYSA-N Sodium Chemical compound [Na] KEAYESYHFKHZAL-UHFFFAOYSA-N 0.000 description 1
239000002250 absorbent Substances 0.000 description 1
230000002745 absorbent Effects 0.000 description 1
239000003242 anti bacterial agent Substances 0.000 description 1
229940088710 antibiotic agent Drugs 0.000 description 1
239000001045 blue dye Substances 0.000 description 1
CDQSJQSWAWPGKG-UHFFFAOYSA-N butane-1,1-diol Chemical compound CCCC(O)O CDQSJQSWAWPGKG-UHFFFAOYSA-N 0.000 description 1
KDYFGRWQOYBRFD-NUQCWPJISA-N butanedioic acid Chemical compound O[14C](=O)CC[14C](O)=O KDYFGRWQOYBRFD-NUQCWPJISA-N 0.000 description 1
159000000007 calcium salts Chemical class 0.000 description 1
229910052799 carbon Inorganic materials 0.000 description 1
238000005266 casting Methods 0.000 description 1
238000004587 chromatography analysis Methods 0.000 description 1
239000007979 citrate buffer Substances 0.000 description 1
239000008367 deionised water Substances 0.000 description 1
229910021641 deionized water Inorganic materials 0.000 description 1
MSJMDZAOKORVFC-UAIGNFCESA-L disodium maleate Chemical compound [Na+].[Na+].[O-]C(=O)\C=C/C([O-])=O MSJMDZAOKORVFC-UAIGNFCESA-L 0.000 description 1
230000000694 effects Effects 0.000 description 1
210000003743 erythrocyte Anatomy 0.000 description 1
HXQVQGWHFRNKMS-UHFFFAOYSA-M ethylmercurithiosalicylic acid Chemical compound CC[Hg]SC1=CC=CC=C1C(O)=O HXQVQGWHFRNKMS-UHFFFAOYSA-M 0.000 description 1
229940005660 ethylmercurithiosalicylic acid Drugs 0.000 description 1
239000012362 glacial acetic acid Substances 0.000 description 1
238000010438 heat treatment Methods 0.000 description 1
150000002484 inorganic compounds Chemical class 0.000 description 1
229910010272 inorganic material Inorganic materials 0.000 description 1
238000000465 moulding Methods 0.000 description 1
150000002896 organic halogen compounds Chemical class 0.000 description 1
UWJJYHHHVWZFEP-UHFFFAOYSA-N pentane-1,1-diol Chemical compound CCCCC(O)O UWJJYHHHVWZFEP-UHFFFAOYSA-N 0.000 description 1
239000004033 plastic Substances 0.000 description 1
ULWHHBHJGPPBCO-UHFFFAOYSA-N propane-1,1-diol Chemical compound CCC(O)O ULWHHBHJGPPBCO-UHFFFAOYSA-N 0.000 description 1
238000000926 separation method Methods 0.000 description 1
239000003381 stabilizer Substances 0.000 description 1
NBOMNTLFRHMDEZ-UHFFFAOYSA-N thiosalicylic acid Chemical compound OC(=O)C1=CC=CC=C1S NBOMNTLFRHMDEZ-UHFFFAOYSA-N 0.000 description 1
229940103494 thiosalicylic acid Drugs 0.000 description 1
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1

Classifications

- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44704—Details; Accessories
- G01N27/44747—Composition of gel or of carrier mixture

Landscapes

Chemical & Material Sciences (AREA)
Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Molecular Biology (AREA)
Analytical Chemistry (AREA)
Chemical Kinetics & Catalysis (AREA)
Electrochemistry (AREA)
Physics & Mathematics (AREA)
Dispersion Chemistry (AREA)
Biochemistry (AREA)
General Health & Medical Sciences (AREA)
General Physics & Mathematics (AREA)
Immunology (AREA)
Pathology (AREA)
Medicinal Preparation (AREA)
Investigating Or Analysing Biological Materials (AREA)

JP60501380A 1984-03-12 1985-03-12 ヘモグロビン変異体の分離のための電気泳動方法およびそれに使用する改良電気泳動ゲル Granted JPS61500810A (ja)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
US58885084A	1984-03-12	1984-03-12
US588850		1984-03-12

Publications (2)

Publication Number	Publication Date
JPS61500810A JPS61500810A (ja)	1986-04-24
JPH0478139B2 true JPH0478139B2 (it)	1992-12-10

Family

ID=24355558

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
JP60501380A Granted JPS61500810A (ja)	1984-03-12	1985-03-12	ヘモグロビン変異体の分離のための電気泳動方法およびそれに使用する改良電気泳動ゲル

Country Status (3)

Country	Link
EP (1)	EP0183711A1 (it)
JP (1)	JPS61500810A (it)
WO (1)	WO1985004251A1 (it)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US5171410A (en) *	1989-10-20	1992-12-15	Helena Laboratories Corporation	Method and formulation for creating kinase isoform separation
WO1991019489A1 (en) *	1990-06-08	1991-12-26	The Upjohn Company	METHOD FOR REGULATING RUMINAL pH
EP2343544B1 (en) *	2009-12-25	2015-03-11	Arkray, Inc.	Method for analyzing hemoglobin by electrophoresis

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
DE2826895A1 (de) *	1978-06-19	1980-01-03	Sebia Sa	Platten fuer die elektrophoretische trennung von lipoproteinen und verfahren zu ihrer herstellung
US4209372A (en) *	1979-06-01	1980-06-24	Corning Glass Works	Alkaline agarosse gel electrophoresis of hemoglobins
US4222836A (en) *	1979-06-01	1980-09-16	Corning Glass Works	Acidic agar gel electrochromatography of glycohemoglobins
US4319976A (en) *	1981-01-19	1982-03-16	Beckman Instruments, Inc.	Electrophoretic technique for separating serum proteins and improved electrophoretic gel for use therein
US4321121A (en) *	1981-01-19	1982-03-23	Beckman Instruments, Inc.	Electrophoretic technique for separating lactate dehydrogenase isoenzymes and improved electrophoretic gel for use therein

1985
- 1985-03-12 WO PCT/US1985/000407 patent/WO1985004251A1/en not_active Ceased
- 1985-03-12 JP JP60501380A patent/JPS61500810A/ja active Granted
- 1985-03-12 EP EP19850901737 patent/EP0183711A1/en not_active Withdrawn

Also Published As

Publication number	Publication date
JPS61500810A (ja)	1986-04-24
EP0183711A1 (en)	1986-06-11
WO1985004251A1 (en)	1985-09-26

Publication	Publication Date	Title
Rawjee et al.	1994	A Peak Resolution Model for the Capillary Electrophoretic Separation of the Enantiomers of Weak Acids with Hydroxypropyl. beta.-Cyclodextrin-Containing Background Electrolytes
Wrigley	1971	[38] Gel electrofocusing
US4292154A (en)	1981-09-29	Buffer composition and method for the electrophoretic separation of proteins
EP0155833A2 (en)	1985-09-25	Element for electrophoresis
JP2004517339A (ja)	2004-06-10	キャピラリー電気泳動によるタンパク質の分離法およびキャピラリー電気泳動用の緩衝組成物
US4321121A (en)	1982-03-23	Electrophoretic technique for separating lactate dehydrogenase isoenzymes and improved electrophoretic gel for use therein
US4319976A (en)	1982-03-16	Electrophoretic technique for separating serum proteins and improved electrophoretic gel for use therein
JPH0478139B2 (it)	1992-12-10
Skude et al.	1972	Thin layer electrofocusing followed by electrophoresis in antibody containing gel
WO1995007458A1 (en)	1995-03-16	Utilization of additives and defined storage systems to increase the stability and performance of electrophoresis media
EP0115436A2 (en)	1984-08-08	Medium for electrophoresis
Merz et al.	1972	Segregation of membrane components using isoelectric focusing in polyacrylamide gels
US4696958A (en)	1987-09-29	Electrophoretic technique for separation of lipoproteins and electrophoretic gel for use therein
JPS6352341B2 (it)	1988-10-18
US4808288A (en)	1989-02-28	Electrophoretic gel for separating hemoglobin variants
Ambler	1978	Isoelectric focussing of proteins on cellulose acetate gel membranes
EP0189422B1 (en)	1989-08-16	Electrophoretic technique for separating hemoglobin variants and electrophoretic gel for use therein
US4321119A (en)	1982-03-23	Buffer composition and method for the electrophoretic separation of proteins
EP0423953B1 (en)	1996-11-20	Method for creative kinase isoform separation
JP2004517338A (ja)	2004-06-10	アルカリ性ｐＨを有する自由溶液におけるキャピラリー電気泳動法
Pflug	1985	Wedge‐Shaped ultrathin polyacrylamide and agarose gels for isoelectric focusing: A new method for typing phosphoglucomutase (PGM1) in semen stains and vaginal swabs
Acevedo et al.	1995	Electrophoretic size separation of proteins treated with sodium dodecyl sulfate in 1% agarose gels
Janssen et al.	1988	Haptoglobin Subtyping in Bloodstains
Lobert et al.	1994	Method for rapid electrophoretic transfer of isoelectric focusing gels to polyvinylidene difluoride
SU1681224A1 (ru)	1991-09-30	Состав мембраны твердофазного ионоселективного электрода дл определени содержани ионов ртути (II)